163 results about "Plant Immunity" patented technology

The invention discloses a eugenol biological pesticide which comprises the following ingredients in percentage by weight: 0.1% to 70% of eugenol, 6% to 20% of surfactant, 0.01% to 0.5% of plant immunity regulator, 10% to 50% of synergist, and raw materials of alcohol and turpentine for the rest part. With the advantage of reasonable formula, the eugenol biological pesticide is characterized in that the active volatility of the active ingredient eugenol is improved, the pesticide effect of the eugenol is enhanced, pesticide doses are reduced, and the pesticide cost for farmers is reduced; pureplant solvents are used in the eugenol biological pesticide which belongs to low-carbon and environment-friendly pesticide, so pollution and harm to the environment are reduced, and the eugenol biological pesticide is safe and environment friendly for use. The eugenol biological pesticide is used for preventing and treating powdery mildew, botrytis and downy mildew and regulating plant immunity, and has the effects of synergy, simultaneous treatment, resistance prevention, resistance delay, etc.

The invention discloses a nutritional-type fertilizer. The fertilizer is prepared from following raw materials in parts by weight: 35-40 of gypsum, 25-30 of bentonite, 13-16 of diammonium phosphate, 10-13 of potassium fulvate, 1-2 of ammonium molybdate, 1-3 of copper sulfate, 1-2 of ferrous sulfate, 3-4 of boric acid, 2-3 of polyvinyl alcohol, 4-6 of chitosan, 3-7 of sodium alginate, 8-12 of a soil conditioner and a proper amount of water. The fertilizer adopts the potassium fulvate and plant essential nutrient elements as components. The ratio of the plant nutrients is reasonable. The fertilizer has characteristics of capability of allowing premature ripening and preventing premature senility for grain crops, vegetables, fruits and economic crops. The fertilizer prepared from the chitosan and the boric acid not only is capable of killing pests, preventing diseases for plants and functioning as a fertilizer, but also is capable of decomposing animal and plant residual bodies and trace metal elements, thus converting the residual bodies and the trace metal elements into nutrients for the plants, enhancing plant immunity and promoting plant health. The bentonite has strong moisture adsorption capability, and can absorb water having a volume of 8-20 times of the volume of the bentonite itself and swell to 30 times in volume. The bentonite has certain glutinousness, thixotropy and lubricity. The blend of the bentonite and silt has plasticity and caking property, and has strong cation exchange capability and adsorption capability.

The invention discloses a plant immune activator protein FoPII1 secreted by fusarium oxysporum and its application. The protein is capable of inducing plant resistance and can be applied as a plant immune activator to the field of plant disease control. An amino acid sequence of the protein is shown in SEQ ID NO: 2. The high-concentration protein can be obtained by using engineering strain expression, which can be used to improve the disease resistance of tobacco and soybean, and the involved plant diseases are tobacco mosaic disease and soybean root rot. The plant immune activator protein notonly can significantly improve plant disease resistance, but also has the advantages of low required concentration, quick effect and long acting time. The FoPII1 provides a novel way to improve plantdisease resistance and control plant diseases.

The invention provides a plant protective method and application of inducing immune system of plant by 4-methyl-1,2,3-thiadiazolyl-5-formic acid (NK-F001), sodium 4-methyl-1,2,3-thiadiazolyl-5-formate (NK-F002), 4-methyl-1,2,3-thiadiazolyl-5-ethyl formate (NK-F003) and methenaimde (SZG-7) to generate resistance against the plant virus. The invention also provides an application for improving the plant immunity by combination of two or three of 4-methyl-1,2,3-thiadiazolyl-5-formic acid (NK-F001), sodium 4-methyl-1,2,3-thiadiazolyl-5-formate (NK-F002), 4-methyl-1,2,3-thiadiazolyl-5-ethyl formate (NK-F003), methenaimde (SZG-7), benzthiadiazole (activated ester, BTH), tiadinil (TDL), DL-Beta-aminobutyric acid, virazole, ningnanmycin, antofine, Bingduxing No.1, Bingduxing No.2, XY-13 and XY-30 so as to induce the plant to generate antiviral activity, processing dosage forms of single and mixture preparations, and compositions and contents of effective components in the dosage forms. The invention discloses a plant inducing range and plant antiviral inducing range of compounds and compositions thereof, and application of plant protection in the fields of agriculture and horticulture thereof.

The invention discloses an Erwinia carotovora pv. Carotovora strain Ecc 36 with the preservation number of CGMCC No. 11751; plant immune activator protein HrpNEccPR secreted by the Erwinia carotovora pv. Carotovora strain Ecc 36 has an effect of preventing and controlling plant soft rot. The invention also discloses a protein sequence of the plant immune activator protein HrpNEccPR and a gene sequence that expresses the protein, and further provides a method for obtaining the plant immune activating protein. According to the invention, an activator protein crude product generated by the selected strain with the preservation number of CGMCC No. 11751 has a good antagonistic effect for plants, especially for Chinese cabbage soft rot, and the activator protein crude product can effectively prevent and control the occurrence of the Chinese cabbage soft rot.

The invention relates to penicillium protein elicitor EPTP and application thereof in improving plant disease resistance. The protein sequence of the EPTP protein elicitor is shown as SEQ ID NO.2, and the molecular weight is 19247 Da. EPTP induces significant increase of activity of phenylalanine ammonialyase, peroxidase and polyphenol oxidase, and when the elicitor induces tobacco anaphylactic reaction, an anaphylactic reaction marker gene hin1 is induced to express; it is indicated through semiquantitative RT-PCR results that EPTP can induce tobacco to obtain systemic acquired resistance, cause up-regulation of the tobacco blade resistance gene transcription level, and induce expression of resistance gene related to the salicylic acid path and the jasmonic acid path. After being applied, EPTP can improve resistance to viruses and pathogenic bacteria by plants, takes effects efficiently, does not pollute environment, reduces dosage of chemical pesticide and has wide application prospects as a plant immunity activator.

The invention discloses a functional healthy medical fertilizer for activating plant immunization, facilitating growth, obstructing virus transmission by insects, which essentially consists of 1-18 percent of sodium alginate, 0.5-12ppm of salicylic acid, 0.5-12ppm of lactone of zinc sulfate and 1-10 percent of brassin according to quality percentage. When applied to transplanted seedlings for root soaking and leaves surface spraying, the solution of medical combination of the invention shows remarkable biological activities: (1) being able to facilitate development of a root system and inhibiting infection to the root system from soil borne diseases, (2) obstructing the virus transmission of insect vectors, being able to replace or remarkably reduce the use of chemical insecticide on the field and presenting huge economic, environmental and social benefits, (3)inhibiting the infection of the virus, (4) activating functions of an immune system of solanaceae plants, presenting curative effects for virus diseases and securing vigorous growth of plants.

The invention discloses a microorganism fertilizer for promoting crop growth and a preparation method therefor. The microorganism fertilizer comprises the components in parts by weight: 100 parts of plant stalks, 0.08-0.16 part of 5A-hydroxyl laxogenin, 0.04-0.10 part of theobromine, 60-80 parts of chitosan hydrochloride, 0.8-1.2 parts of bacillus subtilis bacterium, 6-10 parts of lactobacillus powder, 4-8 parts of yeast powder, 8-20 parts of potassium dihydrogen phosphate, 6-10 parts of ferrous sulfate, 10-15 parts of calcium carbonate, 1-3 parts of magnesium chloride, 1-3 parts of molybdate, 1-3 parts of zinc sulfate, 1-3 parts of manganese sulfate and 1-3 parts of boric acid. The microorganism fertilizer disclosed by the invention comprises 5A-hydroxyl laxogenin and theobromine, the two components have mechanisms that promote growth, and compound use of the two components can further play synergy, so that the crop growth is significantly improved. In addition, after the chitosan hydrochloride is dissolved into soil, animal and plant residues and trace metal elements are decomposed, thereby transforming into nutrients of plants, improving plant immunity, and promoting plant health.

The invention discloses a verticillium dahlia secreting type protein elicitor VdPEL1 and application thereof. The protein can improve the plant disease resistance and can be used as an inductor for inducing the plant immunity and can be applied to the field of plant disease prevention and treatment. The amino acid sequence of the protein is shown as SEQ ID NO:2. The engineering strain expression is used for obtaining high small-to-big protein, so that the disease resistance of tobaccos and cotton can be improved; the related plant diseases include tobacco mosaic, gray mold and cotton verticillium wilt. The protein elicitor can obviously improve the plant disease resistance; the effect is fast; the action time is long. The VdPEL1 provides a new path for improving the plant disease resistance.

The invention discloses a composition comprising lentinan and ribavirin for resisting plant virus diseases. The composition comprises the following active ingredients in percentage by weight: 0.5 to 5 weight percent of lentinan and 0.5 to 5 weight percent of ribavirin. The invention also discloses application of the composition to prevention and cure of tomato virus diseases or tobacco virus diseases. The composition of the invention has good effect of preventing and curing the plant virus diseases, can induce and increase plant immunity so as to inhibit virus infection to achieve effects of resisting virus and increasing yield, can be prepared into an aqua preparation, soluble powder and wettable powder, is convenient to spray and has convenient use. The effect is more obvious by adopting the method of the invention to prevent and cure the plant virus diseases.

The invention provides a composition for preventing and treating plant virus diseases as well as a preparation method and application thereof. The composition comprises the following raw materials: aresistance inducer; a surfactant; sodium alginate and a brassinolide compound; wherein the resistance inducer is at least one of lentinan, chitosan and amino-oligosaccharin; wherein the surfactant isat least one of perfluorohexylethyl methyl dimethoxy silane, (3, 3, 3-trifluoropropyl) dichloromethylsilane and trifluoropropyl methyl cyclotrisiloxane. The composition provided by the invention can induce and enhance plant immunity so as to inhibit virus infection; meanwhile, the surfactant has good compatibility and binding capacity with other raw materials; when the composition is sprayed to plants, a film can be rapidly formed on the surfaces of the plants, the firmness of the film can be improved through mutual combination of the sodium alginate and the surfactant, and even if it rains after spraying, the composition cannot be completely scoured by rainwater, so that the pesticide effect retention time is long, and the pesticide application frequency can be reduced.

The invention discloses a method for preparing a plant growth regulator capable of resisting frost and preventing disease, and relates to the technical field of the plant growth regulator. The preparing method comprises the following steps: dissolving wood vinegar and 10 percent of chitosan in water with weight 10 to 15 times of the wood vinegar and the chitosan; and then stirring and dissolving at 25 to 45 DEG C; finally, adding monopotassium phosphate with weight 0.2 to 0.3 times of the chitosan; the production process of the method is very simple and convenient, and easy for industrial production; the plant growth regulator prepared by the method has obvious effects on prompting plant growth and increasing production, and is capable of activating plant cell, improving self-defense capability, being absorbed by the plant, improving the immunity of the plant, observably enhancing the effects of frost resisting and disease preventing, and improving the production and quality of grains, fruits and vegetables, etc.

The agricultural composition for preventing and controlling nematode contains nematicide and chitin. It has the double effects of killing nematode and raising plant immunity and thus has obvious effect of preventing and controlling nematode. The present invention also relates to agricultural composition comprising the nematicide composition and fertilizer with the functions of purifying soil, raising plant resistance, providing plant with nutrients and preventing and controlling nematode and diseases.

The invention provides a method and a reagent set for preventing citrusyellowshoot, and belongs to the field of agricultural plant cultivation. The technical scheme is that the method for preventing citrusyellowshoot comprises the following steps: A, applying quick lime to soil of a citrus orchard according to usage amount of 50-150 kg / mu; B, applying organic fertilizer containing microorganism bacteria to the soil of the citrus orchard according to usage amount of 100-250 kg / mu; C, every 30-40 days, spraying a plant immunity inducer on citrus plants or irrigating the plant immunity inducer on roots, usage dosage being 100-150 g / mu, and continuously using 3-5 times. The quick lime is used to adjust soil acidity, and an organic biological bacterial fertilizer is used in a cooperated manner, to improve soil structure and quality. The biological bacteria is cooperated with the plant immunity inducer to promote root activity of the citrus plants, improve citrus disease resistance, so as to efficiently prevent citrusyellowshoot in a green manner, and promote healthy growth of the citrus plants.

The invention provides a plant immune resistance inducer containing amino-oligosaccharin, which is prepared from the following raw materials in parts by weight: 1-3 parts of amino-oligosaccharin, 0.8-3.5 parts of brassinolide, 0.01-0.1 part of sodium monofluorophosphate, 0.9-2 part of 3-(difluoromethyl)-1-methyl-N-(3, 4, 5-trifluorobiphenyl-2-yl) pyrazole-4-formamide, linear polysaccharide beta-1,0.1-0.8 part of 4-xylan, 0.5-1.5 parts of a trace metal ion compound, 10-20 parts of a nutrient activator and 0.8-3.0 parts of a synergist. The composition is reasonable in formula, easy to prepare,high in storage stability and low in effective component content loss rate; the plant immune resistance inducer containing amino-oligosaccharin is applied to tobacco damping-off, can promote crop health or growth and development, has a relatively strong prevention and treatment effect on tobacco damping-off, and has an inhibition rate of 97.99.6%.

The new type of environment protecting biochemical fertilizer can raise plant's immunity, promote plant growth, improve soil and reduce pollution to soil. The environment protecting biochemical fertilizer contains diatomite, microbe colony, one or several azophoska components, and plant immunity promoter. The fertilizer may be compounded based on different soil environment and different plant growth condition, so as to make full use of the fertilizer.

The invention relates to the technical field of plant genetic engineering, and specifically relates to application of a gene GmLMM1 related to soybean phytophthora resistance. According to the presentinvention, the gene GmLMM1 involved in the regulation of soybean cell death, immune response and phytophthora resistance is cloned in soybean. PTI immune response and phytophthora resistance of plants can be negatively regulated by the GmLMM1 gene; and by reducing the expression of the GmLMM1 gene, PTI immune response of plant can be effectively enhanced, and phytophthora resistance of plants canbe increased. The clone and functional discovery of the GmLMM1 gene provide important gene foundations and theoretical support for the related mechanisms research of phytophthora resistance, providevaluable gene resources for advancing the research and application of plant defense systems and for breeding new soybean varieties with high disease resistance, and has important application value ingenetic engineering breeding of soybean disease resistance.

A recombinant vector which can transform potato plants so as to confer upon the potato plant immunity against potato virus Y necrosis line is disclosed. The recombinant vector according to the present invention comprises a promoter which functions in a potato plant cell; an operably linked leader sequence of RNA4 of cucumber mosaic virus, which is located downstream of the promoter; and an operably linked sequence which encodes a coat protein of potato virus Y necrosis line, which is located downstream of said leader sequence; and the recombinant vector is capable of transforming potato plants.

The invention discloses plant immune induced resistance protein FgPII1 secreted by fusarium graminearum and an application of the plant immune induced resistance protein FgPII1. The protein can activate a plant immune reaction, and can be used as a plant immune inducing factor to be applied to plant disease prevention and control. The amino acid sequence of the protein is as shown in SEQID NO:2. Through excessive expression of engineering bacteria, high-concentration protein can be obtained and can be used for activating a tobacco immune system, so that the disease resistance of soybeans can be improved, and related plant diseases are root rot of the soybeans. The plant immune inducing protein can significantly activate plant immunity and improve the disease resistance of the plants, and is high in speed of taking effect and low in concentration. The FgPII1 provides a new way for improving the disease resistance of the plants and preventing and treating the plant diseases.

The invention provides a plant-immunity resistance inducer and application thereof. The active ingredients of the plant-immunity resistance inducer contain 2-amino-3,5-dichlorobenzoic acid, prinzone and chitosan. In this way, after the plant-immunity resistance inducer is used in harvested citrus, occurrence of harvested citrus immune penicilliosis can be induced, the damage effect of the penicilliosis on the quality of the harvested citrus can be effectively inhibited, and the preservative and fresh-keeping time of the harvested citrus can be prolonged. The plant-immunity resistance inducer is free of toxicity and pollution, capable of directly acting on the harvested citrus, and no pesticide residues are generated; the plant-immunity resistance inducer can effectively prevent anthracnoseof harvested mangoes.

The invention discloses botrytis cinerea secreted protein elicitor BcXyl1 and application thereof. The protein can increase plant disease resistance and can be used as the elicitor for inducing plantimmunity to apply to the prevention and treatment field of plant diseases. The amino acid sequence of the protein is as shown in SEQ ID No. 2. High-concentration purified protein can be obtained by using engineering strain expression. The BcXyl1 can be used for increasing the disease resistance of tobacco and tomatoes, and the related diseases are tobacco mosaic virus, tobacco gray mold and tomatogray mold. The protein elicitor is capable of evidently increasing the disease resistance of plants, quick in action and lasting in effect. The BcXyl1 provides a new approach for increasing the disease resistance of plants.

The invention relates to a pesticide composition capable of preventing and treating plant virus diseases and application of the pesticide composition, belongs to the pesticide compositions, and aims at providing a pesticide composition capable of preventing and treating plant virus diseases. The active components of the pesticide composition capable of preventing and treating plant virus diseases comprises lentinan and emodin monomethyl ether according to a weight ratio of (1-100) to (0.1-500). Furthermore, the invention also discloses application of the pesticide composition capable of preventing and treating plant virus diseases in prevention and treatment of plant virus diseases. According to the pesticide composition capable of preventing and treating plant virus diseases, lentinan and emodin monomethyl ether are compounded and used as active components; the components are environmentally friendly; the pollution to the environment is avoided; meanwhile, lentinan and emodin monomethyl ether have synergetic effects, have a good effect of preventing and treating the plant virus diseases and are capable of inducing and enhancing plant immunity, so that the infection of the virus can be inhibited; the effects of resisting virus and increasing yield can be achieved.

The invention discloses a plant immunity resistance inducer prepared by using shrimp and crab shells and a preparation method thereof. The plant immunity resistance inducer comprises, by weight, 3-8 parts of modified chitin, 0.5-1 part of traditional Chinese medicine extracts, 0.1-0.5 part of phosphate, 0.1-0.5 part of organic acid and 50-150 parts of water. The preparation method comprises the steps of mixing the modified chitin, the traditional Chinese medicine extracts, the phosphate, the organic acid and the water to homogeneity based on the weight ratio, conducting spray drying on the mixed solution, and collecting the dry material which is the plant immunity resistance inducer. The the chitin originating from the shrimp and crab shells is subjected to structural modification to produce a modified chitin with long distance signal conducting capability and high resistance to inducing plants. The plant immunity resistance inducer using the chitin as the main material possesses a positive immunity effect to the infection of multiple pathogens, particularly to the root rot fungus and phytophthora of tomatoes, and the disease incidence rate is lower than 5%.

A biological pesticide formula is disclosed. The formula comprises following raw materials by weight: 8-12 parts of a java brucea fruit extract product, 1-3 parts of a plant immunity regulator, 6-8 parts of oxymycin, 15-25 parts of mint, 6-10 parts of ethanol, 6-8 parts of polyoxyethylene, 4-6 parts of 1-octanol, 8-12 parts of alum and 6-8 parts of xylene. The pesticide is used for treating and preventing powdery mildew, gray mold and downy blight and regulating plant immunity, and achieves effects of increasing the efficiency, treating a plurality of diseases at the same time, resisting drug resistance, delaying drug resistance, and the like.

The invention relates to an antiviral polypeptide and a preparation method and application of the antiviral polypeptide. The antiviral polypeptide contains any one amino acid sequence shown in SEQ IDNO.1-SEQ ID NO.8, the antiviral polypeptide gene is transformed into a plant and has a remarkable antiviral effect, and the antiviral polypeptide gene has a relatively strong antiviral effect on various viruses such as cucumber mosaic virus and barley stripe mosaic virus. The antiviral polypeptide is prepared by adopting a yeast expression system, the expression polypeptide is sprayed to a surfaceof the plant, and the virus infection amount of the plant is remarkably reduced. The antiviral polypeptide provided by the invention can be used for preventing and treating various virus infection ofplants; plant protection is carried out in production by utilizing a biological agent, and effective plant immune defense is realized, so that the use amount of pesticides is reduced, the plant quality is effectively improved, the yield of the plant is effectively increased, and the economic and social effects of crops are improved.

The present invention is the preparation process of plant growth regulator capable of promoting crop's growth and raise crop's yield. The preparation process includes dissolving organic acid or acid anhydride in water of 50-500 times via stirring, dissolving chitosan in the same amount as the organic acid or acid anhydride via stirring at 15-55 deg.c, dissolving urea and potassium dihydrogen phosphate in 0.2-0.5 time chitosan weight, adding ethanol in 30-50 times the chitosan weight and adding small amount of levulose acid. The plant growth regulator is easily to be absorbed by plant to promote plant growth via activating plant cell, raising plant resistance and raising plant immunity.

The invention discloses a wild grape planting method which includes the steps: (1) seedling bed arrangement: leveling gullies on quasi-viscous and quasi-sandy soil, stacking a layer of 15-20cm high base fertilizers, backfilling the soil and finally stacking a seedling bed at the gullies; (2) interplanting management: shearing wild grape shoots and honeysuckle shoots into 4-12cm long cuttings, soaking the cuttings in growth conditioners for 12-24 hours, and cutting the cuttings into the seedling bed; (3) flower and fruit management: respectively spraying plant immunity resistance inducers to wild grapes 10-11 days before flowering and 3-4 days after flowering, thinning fruits after fruit setting and bagging the fruits for 30-45 days when the diameter of each fruit is 0.2-0.4cm. Seedlings are raised by the planting method of mixed cuttage of the wild grapes and honeysuckle, the occurrence rate of diseases and pests in the growth process of the wild grapes can be obviously reduced, usage of pesticides is decreased, and the yield of the wild grapes is improved.

The invention discloses a botanical fungicide for preventing and controlling tobacco mosaic virus. The botanical fungicide is prepared from the whole-plant mixed extracts of four plants, namely, herba asari, syzygium aromaticum, veratrum nigrum and stellera chamaejasme, as well as an additive and a solvent. Tests show that the botanical fungicide has a good inhibiting effect for the tobacco mosaic virus; either root-irrigation or spraying is adopted, indoor box plant tests show that the protection function is all 43% or above, and the inhibition rate in the therapeutical effect is all 73% or above; field tests show that the control efficiency is 68% or above, and is 20 percentage points higher than that of a chemical disinfectant. The raw materials adopted by the botanical fungicide are all natural plants which can be degraded in nature, no residue is left, and environmental harmony is achieved; besides antimicrobial active ingredients, the plant extracts also include many other ingredients which have the advantages and characteristics of being capable of promoting the plant growth, improving the plant immunity, increasing the disease resistance and the like, and have great significance in reducing the use of chemical pesticides, realizing environmental protection, and producing green and safe agricultural products.

The invention discloses a high-potassium type macroelement water-soluble fertilizer and a manufacture method thereof. The high-potassium type macroelement water-soluble fertilizer is prepared from the following compositions in parts by weight: 2-3 parts of lysozyme, 2-3 parts of lywallzyme, 5-6 parts of brown sugar, 3-5 parts of chitosan, 2-3 parts of plant vinegar, 3-5 parts of citric acid, 1-2 parts of oxalic acid, 0.01-0.05 part of a surfactant, 10-15 parts of polyvinyl alcohol, 20-30 parts of sodium nitrate, 40-50 parts of potassium sulfate, 30-35 parts of potassium dihydrogen phosphate, 3-5 parts of magnesium sulfate, 2-3 parts of ferrous sulphate, and 210-270 parts of water. The high-potassium type macroelement water-soluble fertilizer is complement in nutrition elements, slowly releases nutrition elements after being used, realizes long-acting providing of nutrients for plants, also possesses bacteriostasis and is capable of improving plant immunity.