55 results about "Folsomia candida" patented technology

The present invention relates to biocatalysts that are cells, optimally of the Crabtree-negative phenotype, comprising expression vectors encoding genes heterologous to the cell that enable increased production of organic products. More specifically, the invention relates to genetically modified Candida cells, methods for making the Candida cells, and their use in production of organic products, particularly lactic acid.

The invention relates to a herbal antiseptic containing chitosan. The antiseptic comprises the following components in percentage by weight: 0.05% of essence, 0.5% of CO-400, 3% of kathon, 0.02% of chitosan, 0.3% of critic acid, 0.5% of sodium citrate, 25% of deloncide, 0.5% of honeysuckle flower extract, 0.5% of sophora flavescens extract, 1.5% of glycerin, and the balance being purified water, and the antiseptic is prepared by evenly mixing the components mentioned above. The herbal antiseptic has the advantages that: (1) the antiseptic solution has a weak acidic property, and the pH value of the solution is in a range of 4 to 7; (2) the antiseptic has a prominent and long-lasting inhibiting effect on bacteria and fungi, the candida albicans inhibiting rate is more than or equal to 90%, the staphylococcus aureus inhibiting rate is more than or equal to 90%, and the colibacillus inhibiting rate is more than or equal to 90%; (3) the antiseptic has a stable property, and does not disintegrate or change color.

The invention relates to a Chinese herbal medicinal wet wipe for cleaning and disinfection of the skin, which relates to the technical field of daily necessities. The wet wipe comprises a wet wipe solution and a carrier for the wet wipe solution. The invention is characterized in that raw materials for preparation of the wet wipe solution comprise, by weight, 0.5 to 1% of lavender essential oil, 0.4 to 0.8% of cassia twig essential oil, 0.3 to 1% of cablin patchouli herb essential oil, 0.01 to 0.1% of menthol, 0.01 to 0.2% of paeonol, 0.3 to 1% of a coptis extract, 0.2 to 1% of a witch hazel extract, 0.5 to 2% of hydrogenated castor oil and 5 to 10 % of ethanol, with the balance being water. According to the invention, a non-woven fabric is used as the carrier; the wet wipe is convenient to use and can kill common pathogenic bacteria like escherichia coli, staphylococcus aureus, bacillus subtilis, aspergillus flavus, Gram-positive cocci, shigella dysenteriae, helicobacter pylori and Candida albicans; the wet wipe is mild-natured, natural and safe because of utilization of a Chinese herbal medicine formula and can overcome the problems of irritation and corrosivity of currently-used chemical disinfection products.

The invention discloses a special probe and a gene chip for identifying pathogenic candida. The special probe for identifying the pathogenic candida consists of at least one of the following 24 DNA fragments: 12 DNA fragments of which the nucleotide sequences are respectively sequence 1 to sequence 12 in a sequence table, and 12 DNA fragments of which the nucleotide sequences are respectively reverse complement sequences of sequence 1 to sequence 12 in a sequence table. The invention further provides the DNA chip containing the special probe for identifying the pathogenic candida. The specialprobe, the DNA chip or a device all can be applied to identifying the pathogenic candida. The identification procedure comprises the following steps: enveloping the species-specific probe on a low-density gene chip membrane, carrying out flow-through hybridization and color development on an ITS area of rDNA of the candida to be tested and the species-specific probe on the low-density gene chip membrane by a test platform based on a flow-through hybridization technology, and finally identifying the candida to be tested. The special probe and the gene chip have the characteristics of strong andfast specificity, and fast and specific positive effect in the case of detection of the common pathogenic candida in a clinical specimen and identification of a candida strain.

The invention provides an antibiotic fiber. The antibiotic fiber comprises, by weight, 20-30% of chitosan, 3-5% of a dispersant, 5-10% of a fusogen, 2-5% of a grafting modifier, and the balance PET. The invention also provides a preparation method of the antibiotic fiber, and a pillow core and a quilt core containing the antibiotic fiber as a filling material. Present antibiotic products have safety performance problems and defects which are not adapted to industrial production, so the antibiotic fiber of the invention which can highly effectively inhibit more than twenty harmful bacteria of escherichia coli, staphylococcus aureus, Candida albicans and the like with an average bacteriostasis rate of equal to or more than 98%, and can not generate side effects to beneficial flora in human bodies has the effect of ecological bacteriostasis, and is adapted to industrial production.

The invention discloses a biological disinfectant with safety, high efficiency and low irritation. One milliliter of the biological disinfectant comprises the following components: 0.5 to 10 milligrams of epsilon-polylysine, 1 to 20 milligrams of glycerol, 0.8 to 16 milligrams of dodecyl dimethyl betaine and the balance of buffer solution with the pH value of 6.0 t 7.5. The biological disinfectant can be used for disinfecting hand skin, the surfaces of objects and the like. The sterilizing log value of the biological disinfectant on Escherichia coli and candida albicans within 3 minutes is more than 3. The sterilizing log value of the biological disinfectant on pseudomonas aeruginosa and staphylococcus aureus is more than 6. As the contact lasts, a disinfecting effect becomes better.

The invention discloses an oral cavity washing and caring product with a function of resisting candida albicans. The oral cavity washing and caring product is prepared from the following active ingredients in percentage by mass: 2-15% of artemisia selengensis leave extract, and 1-20% of one or more of lavender extract, radix scutellariae extract and rhizoma curcumae extract. The invention further discloses medicinal toothpaste with a function of resisting candida albicans. The oral cavity washing and caring product has an extremely good inhibition effect for candida albicans, and can be used for preventing and alleviating the oral candidosis disease caused by candida albicans.

There is disclosed a yeast strain of Candida utilis, wherein the yeast strain has been transformed with at least one copy of a gene that is operatively linked to a promoter sequence enabling expression of the gene and encodes a polypeptide having activity of lactate dehydrogenase. The yeast strain is useful for producing lactic acid with high efficiency.

The invention relates to a traditional Chinese medicine composition for preparing Candida resisting medicines. The traditional Chinese medicine composition contains the ingredients in parts by weight: 40-80 parts of cinnamaldehyde and 30-60 parts of pogostone. The traditional Chinese medicine composition for preparing the Candida resisting medicines, provided by the invention, can be used for treating deep or systemic fungal infections caused by Candida, including deep or systemic fungal infections caused by Candida albicans, Candida tropicalis, Candida krusei or Candida glabrata. According to the traditional Chinese medicine composition, the pogostone is screened from numerous ingredients of ageratum oil, has relatively high bacteriostasis activity and has a good synergistic effect with the cinnamaldehyde; the cinnamaldehyde and the pogostone are suitable for being used after being combined; compared with the bacteriostasis activity after the cinnamaldehyde and the ageratum oil are combined, the bacteriostasis activity after the pogostone and the cinnamaldehyde are combined is remarkably improved, and the safety is good.

The invention relates to alpha-pyrone compounds, and a preparation method and application thereof. The alpha-pyrone compounds with novel structure are prepared by nocardiopsis dassonvillei HR10-5 extracted from sea samples. Tests prove that the compounds can be used as a candida albicans inhibitor.

The invention provides a test method for detecting the potential toxic influence of stomach poisoning insecticide compounds on folsomia candida. The method is characterized in that an insecticide or transgenic insecticidal protein is added into yeast powder (feed) uniformly and the mixture is directly delivered to folsomia candida; yeast mixed with a protease inhibitor with a concentration of 75 micrograms E-64 / g is used for positive control treatment and feed without any insecticide compounds is used for negative control treatment; potential toxicity of the tested insecticide compounds on spring tails is assessed by comparing the important life parameters such as the survival rate, the head breadth, the body length and the egg laying amount of folsomia candida of the treatment group and the control groups. By using the method, the biological reaction of folsomia candida to potassium arsenate [PA], E-64 and galanthus nivalis agglutinin (GNA) with different concentrations can be tested. It is shown that the different life parameters of folsomia candida show an obvious dose-effect relationship along with the change of the concentrations of PA, E-64 and GNA, so that the effectiveness and the sensitivity of the method are testified.

The invention relates to applications of Folsomia candida heat shock protein hsp70 (F. candida hsp70) gene in soil environment quality monitoring. The inventor finds that the F. candida hsp70 gene can be used for the soil environment quality monitoring. The invention provides a soil environment quality monitoring method. The method comprises the following steps: 1, exposing Folsomia candida in soil to be detected; and 2, detecting the expression level of the hsp70 gene in the exposed Folsomia candida. The method allows the soil quality to be rapidly monitored and diagnosed through treating artificially-cultured soil animal Folsomia candida as an experiment and using the F. candida hsp70 gene expression level within 48h as a soil quality monitoring index. The method can be used for the early stage diagnosis and early warning polluted soil, and the soil quality assessment, and is more sensitive and rapid than traditional toxicity test methods.

The present invention relates to a method and a means of diagnosing Candida infection. In particular, the present invention relates to a method of diagnosing Candida infection comprising the steps of obtaining a biological sample from a subject at risk of, or suspected to be suffering from, Candida infection, contacting the sample with a mannose depleted antigen composition comprising a soluble cytoplasmic antigen preparation comprising antigens of molecular weight 55 kDa, 30 kDa and 20 kDa and detecting the antigen / antibody reaction.

The invention discloses a production method for chitin-cotton-blended knitted fabric for underwear. The production method includes the steps that lactococcus lactis, sphingomonas, bacillus coagulans and bacillus megatherium are prepared into complex microbial inoculants; 1.5D*38-mm chitosan fibers are put into a fermentation culture solution, the inoculants are introduced to carry out fermentation, the inoculum size is 1%, fermentation temperature ranges from 37 DEG C to 39 DEG C, culturing time ranges from 12 hours to 15 hours, 3% to 5% of chitin fibers and the balance cotton are prepared into blended yarns in proportion, the count of the blended yarns is 14.5 Tex, the twist of the blended yarns per 10 cm is 79.0, and then the materials are knitted into the fabric. According to the production method, the technology is simple, operation is easy, the modification effect is good, the antibacterial performance of the chitin-cotton-blended knitted fabric is greatly improved, and particularly the candida resistance is quite high; in addition, wrinkle resistance can be improved, the fabric is more luster accordingly, and the performance can not be reduced along with increasing of the number of washing times.

The invention discloses a health-care vagina gel containing a complex probiotic ingredient, which is prepared by the following method: (1) preparation of lactobacillus rhamnosus fermentation product lysate; (2) preparation of gel: contained in per 100ml of gel are: 15ml of sodium alcohol ether sulphate (AES: 98 percent), 0.18g of sodium dodecyl benzene sulfonate, 5ml of glycerol, 0.5g of beta-glucan, 3ml of lactobacillus rhamnosus ferment lysate, 3ml of amur corktree bark extract, 0.1g of citric acid, 1.0g of menthol, 4g of NaCl, 0.5g of preservative and the balance of distilled water for setting volume to 100ml. The gel disclosed by the invention is mild and non-irritating, and can effectively inhibit inflammatory reaction and promote the repair of vagina mucosa injury, and moreover, thegel has a strong inhibiting effect on candida but does not have an obvious inhibiting effect on probiotics existing in the vagina, and can effectively prevent the destruction of the environment in thevagina.

The invention relates to a candida albicans colloidal gold detecting card which comprises a test strip and a detecting card shell. The test strip comprises a colloidal gold film, a nitrocellulose membrane, a sample pad, a water absorbing pad and a PVC rubber board, wherein the sample pad, the colloidal gold film, the nitrocellulose membrane and the water absorbing pad are sequentially pasted to the PVC rubber board. The sample pad and the colloidal gold film are arranged side by side, one end of the nitrocellulose membrane and the colloidal gold film slightly cross, and the other end of the nitrocellulose membrane and the water absorbing pad slightly cross. The test strip is cut into a long strip which is slightly narrower than the detecting card, then the long-strip-shaped test strip is mounted in the long-strip flat-shell-shaped detecting card shell, the detecting card shell is provided with a sample adding window and a detecting result display window, the sample pad is located at the sample adding window, and the colloidal gold film is located at the detecting result display window. The candida albicans colloidal gold detecting card can be effectively used for measuring candidaalbicans, and is simple in method, convenient and rapid, and results are accurate.

The invention discloses a method for detecting microbial limit of rhubarb powder. First, a rhubarb powder test sample is weighed, and a test solution is configured; then a medium and a buffer solutionrequired by the invention are configured, then a bacterium suspension of five microorganisms of staphylococcus aureus, pseudomonas aeruginosa, bacillus subtilis, candida albicans and aspergillus niger is cultured, then membrane-filter method is used for counting and bacterial colony recovery rate is detected, If the bacterial colony recovery rate is greater than 70%, the test data is proved to bevalid, if the bacterial colony recovery rate is less than 70%, the test results are invalid, it is necessary to re-dilute the test solution and then use the membrane-filter method to detect, so thata effective value is measured and obtained, and at the same time, the accuracy of the test results can be verified through a control bacteria test method. The method is accurate, effective, rigorous and easy to operate, and is suitable for measuring the microbial limit of the rhubarb powder to ensure the safety of clinical use of the rhubarb powder.

The invention discloses a 1, 5-benzodiazepine compound containing thiazolyl and ester and an application of the compound, and a structural formula of the compound is represented by a formula (I). The compound has a broader spectrum and higher activity for inhibiting funguses ad bacteria, and in-vitro antibacterial activity test result shows that the compound has a more obvious activity inhabiting effect and a higher killing effect on two types of funguses including cryptococcus neoformans clinical strains and standard strains as well as candida albicans, has a very high activity inhabiting effect and a very high killing effect on two types of bacteria including positive bacteria, namely staphylococcus aureus, as well as negative bacteria, namely escherichia coli, and can be taken as a candidate molecule and a lead compound for an antibacterial agent; and the structural formula of the compound is represented as the instruction shown.

Provided is a process for continuously producing xylitol in high yield and productivity using a vacuum microfiltration bioreactor containing a fermentation medium for a strain of the genus Candida, which includes: 5 300 g / l of xylose, 1 10 g / l of urea, 1 10 g / l of potassium diphosphate, 0.01 1 g / l of magnesium sulfate, 0.1-10 mg / l of MnSO4.4H2O, 0.1 10 mg / l of CoCl2.6H2O, 0.1 10 mg / l of NaMoO4.2H2O, 0.1 10 mg / l of ZnSO4.7H2O, 0.1 10 mg / l of AlCl3.6H2O, 0.1 10 mg / l of CuCl2.2H2O, 0.01-5 mg / l of H3BO3, 1-100 mg / l of FeSO4 7H2O, 0.1-10 mg / l of ascorbic acid, 1-100 mg / l of biotin, 1-100 mg of choline, 1-200 mg / l of folic acid, 1-100 mg / l of inositol, 1-100 mg / l of nicotinic acid, 0.1-10 mg / l of p-aminobezoic acid, 1-100 mg / l of pantothenic acid, 0.1-10 mg / l of pyridoxine, 10-1,000 mg / l of riboflavin, and 1-100 mg / l of thiamine.

Provided is a process for continuously producing xylitol in high yield and productivity using a vacuum microfiltration bioreactor containing a fermentation medium for a strain of the genus Candida, which includes: 5 300 g / l of xylose, 1 10 g / l of urea, 1 10 g / l of potassium diphosphate, 0.01 1 g / l of magnesium sulfate, 0.1-10 mg / l of MnSO4.4H2O, 0.1 10 mg / l of CoCl2.6H2O, 0.1 10 mg / l of NaMoO4.2H2O, 0.1 10 mg / l of ZnSO4.7H2O, 0.1 10 mg / l of AlCl3.6H2O, 0.1 10 mg / l of CuCl2.2H2O, 0.01-5 mg / l of H3BO3, 1-100 mg / l of FeSO4 7H2O, 0.1-10 mg / l of ascorbic acid, 1-100 mg / l of biotin, 1-100 mg of choline, 1-200 mg / l of folic acid, 1-100 mg / l of inositol, 1-100 mg / l of nicotinic acid, 0.1-10 mg / l of p-aminobezoic acid, 1-100 mg / l of pantothenic acid, 0.1-10 mg / l of pyridoxine, 10-1,000 mg / l of riboflavin, and 1-100 mg / l of thiamine.

The invention relates to the technical field of traditional Chinese medicines, in particular to a formula and a preparation method of a vagina contraction bacteriostasis liquid. According to the technical scheme, the formula consists of the following components by kilogram: 1 to 3kg of fructus cnidii oil, 3 to 6kg of Sanxian spearwort oil, 1 to 3kg of fructus caryophylli oil, 1 to 3kg of borneol,0.5 to 1.5kg of sophocarpidine, 2 to 4kg of Chinese cherry apple oil, 0.5 to 1.5kg of primrose oil, 1 to 3kg of fructus aurantii oil and 40 to 100kg of plant oil. The vagina contraction bacteriostasisliquid is prepared from a plurality of Chinese medicinal materials, is applied locally, and plays a role through mucous membrane absorption; the vagina contraction bacteriostasis liquid is low in cost, can contract smooth muscle of vagina, and has an inhibiting effect on staphylococcus aureus, escherichia coli and candida albicans.

The invention relates to a preparation method for a novel antifungal compound and an antifungal application. The invention discloses a novel compound with a structure formula I as follows, wherein the novel compound is prepared by separating Streptomyces-albolongus which is used as a source; the chemical name is (1beta, 4beta, 4abeta, 8a alpha)-4,8a-dimethyloctahydronaphthalene-1,4a(2H)-diol; the molecular formula is C12H22O2. The invention also provides a preparation method and an application of the compound. The method provided by the invention comprises the following steps: performing fermenting culture on the strain S.albolongus, and then centrifuging, thereby acquiring a culture solution; absorbing the culture solution by a macroreticular resin column, reducing pressure and concentrating the 95% alcohol eluting part, thereby acquiring a crude extract; treating the crude extract with normal phase silica gel, dextrangel LH-20 and reverse phase silica gel column chromatography, thereby acquiring a monomer compound shown as structure I; and adopting nuclear magnetic resonance spectrum and mass spectrum for identifying the structure as the novel compound. An in vitro antifungal activity research proves that the compound I provided by the invention has obvious inhibiting effect to various pathogenic fungi, including candida albicans, candida parapsilosis and cryptococcus neoformans and is expected to be developed into a novel antifungal drug.

The invention belongs to the bio-technical field, and particularly discloses a quick preparation method for a candida albicans specimen for field emission scanning electron microscope. The quick preparation method is characterized in that candida albicans thalluses are added into a glutaraldehyde fixing solution for being fixed; sterile deionized water is adopted to clean; and tertiary butanol with different concentration gradients is adopted for dehydration; a bacteria solution is dropped into pure tertiary butanol to freeze in a refrigerator of 4 DEG C; and after a dried specimen is sprayed with gold in an ion sputtering mode, the morphological structure of a thallus biological membrane can be observed through a scanning electron microscope by regulating and setting parameters. According to the quick preparation method for the candida albicans specimen for field emission scanning electron microscope provided by the invention, excreta of the candida albicans is reduced to affect image-forming quality of the specimen; a phenomenon that some samples are covered and shielded by phosphate particles is eliminated to the greatest extent; tiny deformation of the specimen is reduced and the biological membrane is prevented from dropping slightly; the surface of the thallus biological membrane is prevented from cracks or shrinkage sinking of varying degrees and even breakage; and meanwhile, damages on the surfaces of the thualuses are eliminated.

The invention relates to the technical field of biology, in particular to a culture medium for promoting the synthesis of antibacterial polypeptide gramicidin and a culture method thereof. The culture medium comprises the following raw materials in parts by weight: 15-25 parts of carbon source, 2-3 parts of nitrogen source, 1-3 parts of K2HPO4 and 1-3 parts of MgSO4.7H2O. In the aspect of promoting the synthesis of the linear short-stem peptide in the bacillus brevis, compared with conventional NA and NB culture mediums, the finished product of SAM liquid culture medium can greatly promote the expression of the linear gramicidin synthetase coding gene, and the expression amount is about 7-9 times of that of the conventional NB liquid culture medium, so that the synthesis amount of the linear gramicidin is greatly improved, and for specific performance, the inhibition zone on the pathogenic bacteria of Candida albicans is increased. The linear gramicidin cultured by the culture method not only can meet the research requirements, but also can promote the extraction, purification, development and application of the linear gramicidin.

The invention provides a volatile organic contaminated soil biotoxicity testing device and a using method thereof. The device is composed of a soil sample bottle, a gas washing and testing device bottle and a peristaltic pump, the top of the gas washing and testing device bottle is connected with the bottom of the soil sample bottle through a guide pipe, and the peristaltic pump is arranged on the guide pipe; a guide pipe connected above the soil sample bottle penetrates through a guide pipe insertion opening in the bottle body of the gas washing and testing device bottle and is inserted into the bottle bottom. According to the device, a solution containing volatile pollutants is siphoned into a sponge through a capillary tube, so that the wetness of the sponge is maintained, a humid environment condition required by Folsomia candida is guaranteed, and meanwhile, the avoidance behavior of the Folsomia candida is tested. The device can be used for simultaneously testing the influence of volatile pollutants in air and an aqueous solution on the Folsomia candida, and is high in sensitivity.