40 results about "Enzyme release" patented technology

Selective occlusion of a blood vessel is achieved by selectively damaging endothelial cells at a target location in the blood vessel, resulting in the formation of a fibrin clot proximate to the damaged endothelial cells. Additional fibrinogen can then be introduced into the blood vessel if occlusion is not achieved, as the fibrinogen is converted to fibrin by enzymes released by the exposed thrombogenic tissue and activated platelets. Endothelial cells are selectively damaged using thermal effects induced by ultrasound, by mechanical effects induced by ultrasound, or by mechanical effects produced by a tool introduced into the blood vessel (such as a catheter-based tool). A particularly preferred technique for selectively damaging endothelial cells involves introducing an ultrasound activatable agent into the blood vessel, and causing cavitation in that agent using pulses of high-intensity focused ultrasound having a duration insufficient to induce thermal damage in adjacent perivascular tissue.

Novel pro-drugs and methods for their use to alter the growth and biological characteristics of living cells, tissues, or whole organisms are described. The methods allow for selective activation of the pro-drugs at or near transformant host cells expressing a gene for an enzyme that activates the pro-drugs. Pro-drugs according to a preferred embodiment of the invention are conjugates of a bioactive compound and a chemical group that is capable of being cleaved from the bioactive compound by action of an enzyme. Methods according to this invention include, (a) introducing into targeted cells a gene encoding an enzyme and (b) administering a pro-drug, wherein the enzyme releases the pro-drug from conjugation. In a preferred embodiment of the invention, the gene encoding the enzyme is a marker gene.

The invention discloses a glucose sensor, which comprises a counter electrode and at least one working electrode, wherein the counter electrode and the working electrode are fixedly arranged on a base; and a degradable layer is coated on the outermost layer of the at least one working electrode. By coating the degradable layer on the surface of the working electrode of the sensor, a relay mechanism of sequential enzyme release can be formed, the service life of the sensor can be obviously prolonged compared with the conventional service life of 3-5 days, and the correction frequency of the sensor is reduced compared with that of the conventional sensor. The sensor has the advantages of small size, relatively simple manufacturing process and low manufacturing cost. Since the service life of the sensor is prolonged, a patient needs not to frequently replace the sensor, and the economic burden of the patient is also obviously reduced. The implanted or intrusive real-time detection glucose sensor with long service life and good biocompatibility has broad application prospect in implanted or intrusive medical detection equipment.

The invention provides a membrane assisted separation of glycoprotein all N-linked carbohydrate chain and an identification method thereof, and solves problems of low reaction efficiency and identification of few types of carbohydrates in prior art. According to the invention, a molecular sieve effect of a membrane of 8-12KD is employed to separate carbohydrate chain released from glycoprotein from protein; on a molecular sieve of 10KD, an N-linked carbohydrate chain of the glycoprotein is released by a PNGase F enzyme; the N-linked carbohydrate chain flows out through repeated centrifugation, but the protein is retained on the membrane, thereby realizing separation of the carbohydrate chain. The method has obvious carbohydrate chain separation effect, and avoids by-products caused by non-specific adsorption and chemical reactions; besides, the method has high reaction efficiency and concise reaction steps.

The invention discloses a kind of compound digestive enzyme preparation and its preparation method. The said enzyme preparation is a kind of microcapsules, and was composed of enteric dissolvable digestive enzyme pellets core, its coat, gastric dissolvable digestive enzyme layer and coat of it. Its preparation comprises steps of: (1)blend enteric coated digestive enzymes with accessories, then add solvent, churn up to form wet material; (2)the wet material was shoved ,cut, rolled to form a globose wet grains, dried and the enteric dissolvable digestive enzyme pellets core was screened out; (3)sprinkle solutions of enteric dissolvable coating material on it ,drying to get the enteric dissolvable digestive enzyme pellets core; (4)sprinkle gastric dissolvable digestive enzyme solutions to the core ,then sprinkle the solutions of gastric dissolvable coating material , desiccate and screen out the microcapsules. The compound digestive enzyme preparation has a high enzyme release rate and good effect on therapy in practice.

The invention relates to complex enzyme and application thereof, which belongs to the technical field of the brewing of distillate spirits. The complex enzyme comprises the following enzyme preparations and yeasts: 200 to 225 U / g of saccharifying enzyme (wheat), 80 to 100 IU / g of pullulanase (wheat), 10 to 15 IU / g of acid protease (wheat), 15 to 20 IU / g of cellulose (wheat), 15 to 20 IU / g of beta-dextranase (wheat), 5 to 10 IU / g of beta-mannase (wheat), 10 to 15 IU / g of xylanase (wheat), 5 to 10 IU / g of phytase (wheat), 5 to 10 IU / g of pectase (wheat), 1.5 to 2 percent of high temperature resistant saccharomyces cerevisiae (W / W), and 1 to 1.5 percent of aroma-producing yeast (W / W). The complex enzyme is applied as an additive to the production of wheat type distillate spirits. The complex enzyme can increase fermentable sugar, improve the utilization rate of raw materials, and improve the distillation yield; at the same time, the complex enzyme releases and synthesizes more flavor substances, and has the advantages of improving the quality of the distilled spirits and increasing the high-quality product rate of xiaoqu distillate spirits and so on; and the complex enzyme can be widely applied to the production of wheat type distillate spirits.

The invention discloses a method for breaking the cell wall of microalgae. The method mainly includes cell culture, adding microalgae, releasing hydrolytic enzymes, hydrolyzing the cell wall and taking out the original liquid of the microalgae; The bacteria are cultivated in a predetermined space by the culture medium, and microalgae are added in the cultured space according to the number of the bacteria. Adding microalgae is to add the microalgae to be decomposed into the space for cultivating the bacteria, so that the bacteria The body releases hydrolytic enzymes, which can hydrolyze the cell walls of microalgae. After the decomposition of the enzymes, the cell walls can be converted into sugars, and the cell walls of the microalgae will gradually form holes due to decomposition, while the oil in the microalgae can be When the pore diameter of the hole is larger than the volume of the oil, it floats to the culture medium of the bacterial cell culture, and the stock solution remaining in the microalgae can be taken out and classified for use after the cell wall is completely decomposed.

The invention discloses a micro-RNA-21 ultra-sensitive detection method based on double-enzyme signal cascade amplification. The method includes the steps: horseradish peroxidase enzyme-labeled probepreparation: coupling carboxylation polystyrene micro-spheres and horseradish peroxidase enzyme through DNA (deoxyribonucleic acid) single chains by an EDC (ethylene dichloride) carboxyl and amino coupling method; double-chain specific nuclease assisted target recycling: specifically cutting the DNA single chains complementarily paired with RNA (ribonucleic acid) to be detected in a probe by double-chain specific nuclease, triggering target circular reaction, releasing more horseradish peroxidase enzyme by cutting and amplifying signals; horseradish peroxidase enzyme signal amplification: collecting the horseradish peroxidase enzyme released in supernatant by cutting, adding a TMB (tetramethylbenzidine) chromogenic substrate, realizing naked-eye qualitative diagnosis by color change, and realizing enzyme-labeled quantification according to change of absorbance values. The difference of micro-RNA family members is effectively distinguished, and the method is suitable for community tumorscreening of high risk groups and has great application values in biomedical research and clinical diagnosis.

The invention discloses a soybean milk processing method adopting whole bean enzyme deactivation. The soybean milk processing method comprises the steps of performing whole bean enzyme deactivation, performing wet bean peeling, performing shearing and accurate grinding, performing continuous high-pressure homogenization twice, performing homogenization again after cooling and the like. In the stepof performing whole bean enzyme deactivation, soaked beans are transferred to an enzyme deactivation device, the enzyme deactivation temperature is controlled to be 93-98 DEG C, the beans are spirally propelled, and water of 93-98 DEG C is used for spraying and flushing the beans to a suspended and turnover state; and after continuous propelling for enzyme deactivation for 15-20 minutes, the beans are discharged from the enzyme deactivation device. According to the soybean milk processing method adopting whole bean enzyme deactivation, during enzyme deactivation, spiral continuous propellingand spraying and flushing are combined, so that the beans can be suspended, turned over and heated uniformly, and particularly fibers fully absorb water, expand and soften. After the beans subjected to the whole bean enzyme deactivation are processed into soybean milk, the special fragrance of cooked beans can be generated. On the basis of sufficient enzyme deactivation, harmful microorganisms canbe treated at the same time. The whole bean enzyme deactivation is adopted, enzymes are inactivated before enzyme release (namely beans breaking), and subsequent bad flavors such as beany flavor areeradicated.

The present invention relates to one kind of slow released enzyme micro pill for feed and its preparation process. The slow released enzyme micro pill is prepared through a rolling process, is stable during further feed production, and has its enzyme released slowly or in certain location to produce lasting effect. The present invention is superior to common enzyme preparation, which is instable in the intracorporeal environment.

The invention discloses a petroleum-polluted soil restoration method, belonging to the field of polluted soil treatment. According to the method, cultivated animals are focused and are assisted with feeds, nutrient substances are supplied to petroleum-polluted soil, a surfactant is fed into the petroleum-polluted soil for chemical cleaning so as to transfer pollutants from the surfaces of soil particles into a water phase, so that the contact area between the pollutants and microorganisms is enlarged, and the bio-availability of the pollutants is improved. A plant-planting soil system is capable of accelerating the removal the pollutants, each excreta or enzyme released by the plants is capable of promoting the biodegradation of organic pollutants and strengthening the mineralization effect of rhizosphere microorganisms. Besides, by effectively improving soil conditions of vegetation and improving the gas permeability of soil, the degradation efficiency of the organic pollutants can be improved. The method can be used solving the problems of low degradation efficiency and unthorough degradation effect as a single control condition is adopted in existing microorganism treatment methods and the functions of the microorganisms cannot be adequately exerted.

The invention discloses a method for detecting particular microorganism. The method comprises the following steps: (a) capturing: an antibody corresponding to a particular microorganism type is selected, the particular microorganism in a tested sample is captured through idiosyncratic reaction between the antibody and the microorganism with the particular type; (b) separation: the particular microorganism and the tested sample are separated from each other; (c) enzyme release: catalase release reagent is added in the solution of the separated particular microorganism, so that catalase in the particular microorganism is released and then enters the solution; and (d) detection: peroxide is added in the solution, the concentration of the peroxide is obtained from the detection, so that the concentration of the particular microorganism can be further obtained. The invention has the advantages of high detection accuracy, high detection sensitivity, capability of detecting the particular microorganism, and the like, and can be widely used in food safety detection.

The invention discloses extracellular expression and application of tyrosine phenol lyase and belongs to the technical field of biology. A method for extracellular expression includes: cloning high-activity tyrosine phenol lyase gene, co-expressing with signal peptide signal, converting Escherichia coli competent cells, and building a tyrosine phenol lyase extracellular secretion engineering strain mediated by signal peptide to realize extracellular expression, wherein obtained tyrosine phenol lyase fermentation liquid is used for converting and synthesizing tyrosine and derivatives. An extracellular expression mode is adopted to build the tyrosine phenol lyase engineering strain, and enzyme release quantity reaches higher than 50%, so that toxicity caused to cells due to excessive accumulation of protein in the cells is reduced, and total enzyme activity is improved by 36% when compared with common expression. Part of enzyme generated by fermentation is released into the fermentationliquid, thallus cells are high in permeability, contact of a substrate with enzyme is facilitated, and catalytic efficiency of the enzyme is improved substantially. The fermentation liquid can be directly adopted to catalyze the substrate to product L-tyrosine and the derivatives, and the cells do not need to be broken to extract pure enzyme for reaction, so that using cost of the enzyme is saved.

The present invention provides low-density compositions, as well as particulates formed, at least in part, from such compositions. Preferred low-density materials include, for example, hollowspheres, low-density minerals, and low-density wood materials (e.g., sawdust). The low-density compositions of the invention can be formed as particulates, or cores, suitable for use in forming enzyme granules, e.g., marums, layered granules, prills, drum granules, agglomerated granules, or the like. Granules are disclosed having advantageous properties, e.g., low dusting, storage stable, fast enzyme-release profile, low true density, etc. The granules of the invention are especially useful, for example, in liquid detergents and cleaners, such as predominantly aqueous, liquid laundry detergents. In one embodiment, granules are provided having a true, or volumetric, density within a range of from about 0.95 to about 1.4 g / cm<3>. The granules can be economically produced in commercial quantities by way of a marumerization, drum granulation, fluid-bed spray-coating, pan-coating, or other suitable process.

The invention discloses a summer and autumn tea processing method, tea, a tea bag and drinks. The processing method comprises the following steps: crushing and processing the tedded leaves of the fresh summer and autumn tea leaves after enzyme-deactivating to obtain the broken tea pieces and the tea juice; steaming the broken tea pieces for deactivating enzymes; adding citric acid and / or an ascorbic acid enzyme activity conditioning agent to the tea juice, adding the enzyme, and then coating the broken tea pieces with the tea juice, performing enzymatic hydrolysis at 35 to 45 DEG C for 20 to 60 minutes, and drying the material to obtain the tea. The method synergistically adopts the methods of extrusion wall breaking, steam debittering, directional blunt enzyme release and enzymatic methodto obtain a low-temperature quick-making high-quality tea product having fresh and mellow taste, sweet or sweet aroma, rich tea mouthfeel, and unique flavor. The method effectively solves the resource utilization bottleneck problem of poor adaptability of the summer and autumn tea, and provides the effective method for the large-scale and high-value utilization of the summer and autumn tea.

The invention discloses a homogeneous phase biosensing method for detecting kanamycin and application thereof. The activity of a DNA enzyme is suppressed by a double-strand hybridization reaction between G-quadruplex DNA (S1) and kanamycin nucleic acid aptamer, the corresponding quantity of S1 is released via specific binding of the kanamycin and the nucleic acid aptamer thereof, and is combined with chlorhematin to form the DNA enzyme and perform catalytic chromogenic signal transduction; in addition, after a complementary chain S3 is introduced for hybridization, a hairpin structure formed by the kanamycin and the nucleic acid aptamer thereof can catalyze target analyte circulation via exonuclease III, and thus the sensitivity of the method is improved; a quantitative relation between the absorbancy of a chromogenic product and the concentration of kanamycin analyte is built by using a catalytic chromogenic reaction of the DNA enzyme released by specific recognition of the nucleic acid aptamer. The method provided by the invention is convenient to operate, low in cost, high in sensitivity and good in stability, and has good application value for quick detection of kanamycin residual in complex mediums such as foods and water.

The invention relates to the technical field of fry hatching, in particular to a tilapia mossambica roe hatching circulation system. The system comprises a rack, a hatching pond, a temporary raising pond, a fry pond, hatching barrels, a temporary raising basin, a water storage pond and a water supply pond. By forming a hatching cavity, each hatching barrel can be cultured independently to preventdifferent roe feeding times of the hatching barrel. Egg envelope lytic enzymes released by roes without membranes input into the hatching barrel early to water enter the hatching barrel along with circulating water and roes are put in the hatching barrel to lead to membrane release of roes put in the hatching pond, so that the hatching rate is reduced; the content of dissolved oxygen in water is improved through water circulation, so that the hatching rate is prevented from being reduced as a result of oxygen deficit in the hatching process. The dosage of water can be reduced, so that the system is energy-saving and environment-friendly. By arranging the hatching barrels, the temporary raising basin and the fry pond on the rack to form a three-layered three-dimensional structure, an intactfry hatching culture process can be finished, so that the intermediate transfer links are reduced, the hatching efficiency is improved and the production cost is lowered.

Bound salads and other food products can enjoy decreased separation with the addition of a source of glucomannan, especially at from 0.1 wt% glucomannan and higher. Glucomannan absorbs substantial amounts of moisture released by the inclusions such as tuna fish, potatoes, vegetables etc, and as such, prevents "watering out" of the salad. Moreover, glucomannan is resistant to certain enzymes released by the inclusions such as carrots, cabbage, vegetables, fruits etc. Particularly important in this respect are amylases. By virtue of its resistance to amylase, glucomannan can thicken dressings in light products such as light mayonnaise, which often include starches subject to degradation by amylase, with little or minimal "watering out" in extended use.

A method for extracting organic phenol ester alcohol and sugar fermentation enzyme natural organic vitamin from Mountain Litsea Fragrant Litsea fruit comprises the following steps: A, oscillating the Mountain Litsea Fragrant Litsea fruits by wave radiation energy to intensify components in the fruits, so as to release chemical energy of the fruits and achieve complete cleaning and sterilization of fruit skins; B, screening good fruits for production, fermenting bad fruits with clean water in a fermentation tank, and conducting fruit residue-water squeezing separation until fermentation to a standard; C, subjecting screened good fruits to natural brewing fermentation, ripening phenolic ester alcohol to a standard after fermentation and conduct subsequent process; and D, adding an equivalent amount of pure water to the screened good fruits, crushing and introducing into a cooking unit, ripening, sterilizing, introducing the materials into another fermentation tank, adding pure water for culture fermentation, after decomposition is completed and ripening of enzyme releases alcohol taste, conducting enzyme pomace and water separation process. The enzyme pomace from the separation becomes Mountain Litsea Fragrant Litsea phenolic ester alcohol lees products, and enzyme juice from the separation becomes becomes Mountain Litsea Fragrant Litsea enzyme liquid product.

The invention discloses a soft type endoscope cleaning and disinfecting device. The structure of the soft type endoscope cleaning and disinfecting device comprises an operation panel, an electric control cabinet, universal wheels, a bottom plate, a disinfecting liquid storing chamber, an alcohol window, an enzyme releasing window, a disinfection cavity, a visual window device, a protection strip and an upper cover plate, wherein the operation panel is connected with the electric control cabinet; the universal wheels are arranged below the bottom plate; the alcohol window is formed above the disinfecting liquid storing chamber; the disinfection cavity is formed in an inner framework of the electric control cabinet; the visual window device consists of glass, scraping adhesive tapes, springstrips, a connecting rod, a motor and an intermittent relay; the glass is connected with the protection strip; each scraping adhesive tape is connected with the corresponding spring strip; and an inner plate of the upper cover plate is over against the disinfection cavity. The soft type endoscope cleaning and disinfecting device provided by the invention, the visual window device is arranged, so that the scraping adhesive tapes can be driven through the motor to clean dirt on the glass, the cleanliness is increased, the internal cleaning situation is convenient to check, and the misjudging rate is reduced.

The invention relates to a method for releasing intracellular enzyme during bacterial culture, which comprises the following steps: under a sterile condition, selecting a ring from a bacillus strain preservation slope, inoculating the ring into a liquid culture medium, carrying out shaking culture at 30-37 DEG C at the rotating speed of 180r / min for 24-48 hours, and when bacillus is cultured to a certain OD value, carrying out stress treatment on the bacillus to break thalli, so as to obtain a bacterial strain; therefore, the intracellular enzyme is released. According to the method, various technical problems of enzyme activity damage, high cost, solvent residue and the like in the intracellular enzyme release process are effectively solved, and rapid and efficient release of the intracellular enzyme is realized. The bacterial culture solution rich in intracellular enzyme can efficiently degrade lignin, promote conversion of nitrogen and carbon, quickly degrade organic matters and refractory substances such as lignocellulose raw materials, and can be used for fermenting and degrading agricultural wastes; and the strain can also be synergistically acted with zymophyte and extracellular enzyme to ferment and compost straws, rotten vegetables, garden branches of fruits and vegetables, kitchen garbage and the like and feed the straws.

The invention relates to a sedum aizoo enzyme and a preparation method thereof. The method is characterized in that the preparation method comprises the following steps: preparing fermentation raw materials, conducting mixing and fermentation, planting bacteria for the first time, planting bacteria for the second time, filtering fermentation liquor by using a precise membrane filter, and conducting filling or low-temperature storing so as to obtain the product. The invention has the beneficial effects that: in the preparation process of the sedum aizoon ferment provided by the invention, the nutrient components of the sedum aizoon raw materials are not lost or destroyed, and the prepared sedum aizoon enzyme releases effective substances in the sedum aizoon, remarkably improves the nutrition and health care value, can prevent cardiovascular diseases and improve immunity. In the preparation method provided by the invention, the preparation conditions simulate the natural generation conditions of the enzyme, the principle is scientific, the preparation process is simple, and the preparation process is easy to control and suitable for large-scale production. The obtained sedum aizoo enzyme does not contain any chemical additive, is healthy and safe, has the characteristics of pure nature, can be taken for a long time, is convenient to take, and has good market prospect.

The invention discloses an application of an extract of cyclocarya paliurus in preparing a medicine for treating or preventing diabetes cardiomyopathy, the medicine comprises the extract of cyclocaryapaliurus, and a pharmaceutically acceptable carrier, and the extract of cyclocarya paliurus is an alcohol extract of cyclocarya paliurus. The extract of cyclocarya paliurus has the beneficial effectsthat the alcohol extract of cyclocarya paliurus can lower hypoglycemia, serum total cholesterol and triglyceride in mice, and reduces the amount of LDH, CK, AST myocardial enzymes released by myocytenecrosis in acute myocardial infarction as well as a myocardial necrosis marker-cardiac troponin. The alcohol extract of cyclocarya paliurus can significantly improve the structure and morphology ofdiabetic cardiomyocytes, and reduces the apoptosis of cardiomyocytes.

The invention discloses a whole beans enzyme deactivation method. The method comprises the following steps: transferring soaked beans into a feeding end of an enzyme deactivation device, controlling the enzyme deactivation temperature to be 93-98 DEG C, spirally propelling the beans, during spiral propelling, spraying the beans with water of 93-98 DEG C until the beans are in a suspended overturning state, continuously propelling for enzyme deactivation for 15-20 minutes, and outputting the beans from a discharging end of the enzyme deactivation device. According to the whole bean enzyme deactivation method, during enzyme deactivation, spiral continuous propelling and jetting are combined, beans can be suspended, turned over and heated uniformly, and particularly fibers fully absorb water,expand and soften. In addition, after the beans subjected to whole bean enzyme deactivation are processed into soybean milk, the special fragrance of cooked beans can be generated. The enzyme deactivation time and temperature are combined, and harmful microorganisms can be treated on the basis of sufficient enzyme deactivation. The whole bean enzyme deactivation technology is adopted, enzyme is inactivated before enzyme is released (namely before beans are crushed), and generation of bad flavor in a series of subsequent production processes is eradicated.

The present invention includes a device and method comprising: one or more wells on a substrate onto which one or more molecules of interest (MOI) binding reagents are attached, wherein each of the one or more MOI binding reagents is within a molecular proximity of one or more detectable signal molecules, wherein each of the one or more detectable signal molecules comprise one or more signal molecules that are releasable in the presence of the MOI by one or more enzymes and the signal is detected by an electronic detection system.

Compounds and methods for use in selectively inhibiting a lytic enzyme based on feedback inhibition. The conjugated compound serves as a substrate for a lytic enzyme. Cleavage of the conjugated compound by the lytic enzyme releases an inhibitor of the enzyme.