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Method for detecting particular microorganism

A detection method and microbial technology, applied in the direction of electrochemical variables of materials, analytical materials, instruments, etc., can solve the problems of complexity, long detection time, and low detection sensitivity, so as to reduce the detection cost, increase the complexity, and simplify the detection process. Effect

Inactive Publication Date: 2009-06-03
FOCUSED PHOTONICS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] 1. Among the rapid detection methods currently researched and developed, the detection time is still relatively long, and the detection sensitivity is still relatively low
[0011] 2. These rapid technologies are all aimed at the detection of total bacteria, and there is no report on the detection of a certain type or a certain type of specific bacteria. In practical applications, most of the people need to measure a specific type or a specific type Bacteria, such as Escherichia coli, pathogenic Escherichia coli, etc., because only by knowing the specific type of bacteria, it is possible to take corresponding measures to deal with them
Now, with the development of immune technology, people have also developed rapid immunological detection methods for specific bacteria, but these methods require two antibodies, the first is a detection antibody for a certain type or type of specific bacteria, Then there is an enzyme-labeled antibody for signal detection, which is more complicated

Method used

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  • Method for detecting particular microorganism

Examples

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Effect test

Embodiment 1

[0041] A test method for specific microorganisms for the detection of pathogenic Escherichia coli O157:H7 in food, such as figure 2 As shown, the method includes the following steps:

[0042] a. Capture steps

[0043] selecting antibodies corresponding to Escherichia coli O157:H7, and immobilizing the antibodies on magnetic beads;

[0044]The magnetic beads can be prepared according to the method in the literature (eg Colloids Surf. A: Physicochem. Eng. Aspects 212 (2003) 219), or commercial products can be purchased. The antibodies used can be prepared by standard immunization methods, or commercial products can be purchased; the fixation of antibodies on magnetic beads also adopts the commonly used molecular biological fixation method; there are some commercial products (such as infectious diseases from the Chinese Center for Disease Control and Prevention Commodities provided by Preventive Controls) have E. coli O157:H7 antibodies immobilized on magnetic beads so that th...

Embodiment 2

[0058] A test method for specific microorganisms for the detection of Salmonella in food, such as image 3 As shown, the method includes the following steps:

[0059] a. Capture steps

[0060] Selecting the Salmonella antibody, and immobilizing the antibody on the detection band of the immunochromatographic material;

[0061] One end of the immunochromatographic material is placed in the tested sample solution, and the Salmonella in the tested sample reacts specifically with the antibody on the detection band of the immunochromatographic material, thereby capturing the Salmonella in the sample;

[0062] b. Separation step

[0063] Cut off the part of the detection band on the chromatographic sheet to ensure that the influence of the residue of the sample to be tested on the measurement is eliminated, and at the same time improve the accuracy of the test; put the part of the test band into 1 ml of pH7.2 phosphate buffer to complete The separation of Salmonella and tested sam...

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Abstract

The invention discloses a method for detecting particular microorganism. The method comprises the following steps: (a) capturing: an antibody corresponding to a particular microorganism type is selected, the particular microorganism in a tested sample is captured through idiosyncratic reaction between the antibody and the microorganism with the particular type; (b) separation: the particular microorganism and the tested sample are separated from each other; (c) enzyme release: catalase release reagent is added in the solution of the separated particular microorganism, so that catalase in the particular microorganism is released and then enters the solution; and (d) detection: peroxide is added in the solution, the concentration of the peroxide is obtained from the detection, so that the concentration of the particular microorganism can be further obtained. The invention has the advantages of high detection accuracy, high detection sensitivity, capability of detecting the particular microorganism, and the like, and can be widely used in food safety detection.

Description

technical field [0001] The present invention relates to the detection of microorganisms, in particular to a method for detecting specific microorganisms, such as the detection of bacteria and fungi. Background technique [0002] Food safety has become an issue of great concern to people. According to statistics, microbial contamination, especially harmful and pathogenic microbial contamination, accounts for a large proportion of various food safety problems. Therefore, specialized techniques are required to detect microorganisms in food. [0003] At present, most of the conventional methods for detecting microorganisms in food are to achieve the purpose of detection by means of culturing living cell growth, such as plate colony counting. Although these methods have high detection sensitivity, they all need to be carried out under sterile conditions in the laboratory and require a certain amount of incubation time, so they are cumbersome and time-consuming, requiring 24 hou...

Claims

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Application Information

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IPC IPC(8): G01N7/18G01N27/26
Inventor 吴坚
Owner FOCUSED PHOTONICS
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