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A kind of enzyme catal release drug preparation with double curative effect, its preparation method and application

A curative effect and enzyme-touching technology, which is applied in the field of pharmaceutical preparations and enzyme-catalyzed release drug preparations, can solve the problems of limited treatment depth and difficult heating

Active Publication Date: 2015-08-19
山东则正医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hyperthermia has achieved good results in relieving symptoms and improving knee joint function, and compared with drug therapy, hyperthermia has lower side effects, but its treatment depth is limited, and it is difficult to heat deep parts of the knee

Method used

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  • A kind of enzyme catal release drug preparation with double curative effect, its preparation method and application
  • A kind of enzyme catal release drug preparation with double curative effect, its preparation method and application
  • A kind of enzyme catal release drug preparation with double curative effect, its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The present embodiment prepares the enzyme catalyst release drug preparation with double curative effect according to the following method:

[0022] Step 1. Preparation of polyethylene glycol-gold nanorods: a seed growth method was used to prepare gold nanorods with cetyltrimethylammonium bromide (CTAB) as a stable layer. First, the seed solution was prepared according to the conventional method, and then the seed solution was added to the growth solution under vigorous stirring, and kept for 3 hours. The solution was bright purple, and was centrifuged at 11,000 rpm for 10 minutes to separate and concentrate to obtain CTAB-AuNR. Disperse CTAB-AuNR in 4 mL of polyethylene glycol-thiol (PEG-thiol) aqueous solution with a molecular weight of 5000 at a concentration of 250 μM, stir and incubate for 1 h, dialyze with deionized water for 24 h, and centrifuge to remove CTAB to obtain polyethylene glycol-gold nanoparticles A rod with a long diameter of 40nm and a short diameter...

Embodiment 2

[0037] The present embodiment prepares the enzyme catalyst release drug preparation with double curative effect according to the following method:

[0038]Step 1. Preparation of polyethylene glycol-gold nanorods: a seed growth method was used to prepare gold nanorods with cetyltrimethylammonium bromide (CTAB) as a stable layer. First, the seed solution was prepared according to the conventional method, and then the seed solution was added to the growth solution under vigorous stirring, and kept for 3 hours. The solution was bright purple, and was centrifuged at 11,000 rpm for 10 minutes to separate and concentrate to obtain CTAB-AuNR. Disperse CTAB-AuNR in 4 mL of polyethylene glycol-thiol (PEG-thiol) aqueous solution with a molecular weight of 2000 at a concentration of 250 μM, stir and incubate for 1 h, dialyze with deionized water for 24 h, and centrifuge to remove CTAB to obtain polyethylene glycol-gold nanoparticles Great;

[0039] Step 2. Preparation of methylprednisolo...

Embodiment 3

[0042] The present embodiment prepares the enzyme catalyst release drug preparation with double curative effect according to the following method:

[0043] Step 1. Preparation of polyethylene glycol-gold nanorods: a seed growth method was used to prepare gold nanorods with cetyltrimethylammonium bromide (CTAB) as a stable layer. First, the seed solution was prepared according to the conventional method, and then the seed solution was added to the growth solution under vigorous stirring, and kept for 3 hours. The solution was bright purple, and was centrifuged at 11,000 rpm for 10 minutes to separate and concentrate to obtain CTAB-AuNR. Disperse CTAB-AuNR in 4 mL of polyethylene glycol-thiol (PEG-thiol) aqueous solution with a concentration of 250 μM and a molecular weight of 600 (PEG-thiol), stir and incubate for 1 h, dialyze with deionized water for 24 h, and centrifuge to remove CTAB to obtain polyethylene glycol-gold nanoparticles Great;

[0044] Step 2. Preparation of met...

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Abstract

The invention relates to a pharmaceutic preparation, in particular relates to an enzyme-controlled release pharmaceutic preparation with dual effects, a preparation method and application thereof, and belongs to the technical field of pharmacy. The enzyme-controlled release pharmaceutic preparation comprises parts A, B, C and D, wherein the part A is gold nanorods; the part B is polyethylene glycol; the part C is matrix metalloproteinase specific recognition enzyme; the part D is methylprednisolone. According to the preparation method, a steroidal anti-inflammatory medicament, namely, methylprednisolone, is connected to the surfaces of polyethylene glycol-gold nanorods by using substrate matrix metalloproteinase specific recognition enzyme as a link arm, so as to obtain a methylprednisolone-substrate matrix metalloproteinase specific recognition enzyme-polyethylene glycol-gold nanorods targeted delivery system, and as a result, the enzyme-controlled release pharmaceutic preparation with dual effects of pharmaceutical therapy and thermal therapy is realized.

Description

technical field [0001] The invention relates to a pharmaceutical preparation, in particular to an enzyme catalyst release drug preparation with dual curative effects, its preparation method and application, and belongs to the technical field of pharmacy. Background technique [0002] Rheumatoid arthritis (RA) is a chronic autoimmune disease of connective tissue with unknown pathogenesis. Arthritis causes pain, swelling, stiffness, and loss of function in the bones and joints. There is still no cure for RA, and the clinical treatment includes drug therapy and hyperthermia. Drug treatment is mainly non-steroidal and corticosteroid anti-inflammatory drugs, such as ibuprofen, methylprednisolone succinate (MPS), etc., methylprednisolone is an adrenocortical hormone drug, mainly used for allergic and inflammatory diseases disease. Methylprednisolone is insoluble in water, and the commercially available preparation uses methylprednisolone sodium succinate (methylprednisolone) fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K47/02A61K31/573A61K41/00A61P19/02A61P29/00
Inventor 靳翔
Owner 山东则正医药技术有限公司
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