33 results about "E-polylysine" patented technology

The invention discloses a method for preparing antibacterial edible films. The method includes adding polysaccharide materials and epsilon-polylysine into a stirring ball mill and enabling the polysaccharide materials and the epsilon-polylysine to carry out ball-milling reaction at the temperature of 25-50 DEG C for the reaction time of 5-10min to obtain polysaccharide and epsilon-polylysine compounds; adding water into the polysaccharide and epsilon-polylysine compounds, gelatin and glycerin to prepare film forming liquor; coating the film forming liquor to form the antibacterial edible films. A mass ratio of the polysaccharide materials to the epsilon-polylysine is 5:0.8-2.2. The method has the advantages that other solvents can be omitted in reaction procedures, the reaction time is short, the temperature is low, products have light colors, good film forming effects can be realized, the films obtained by the aid of the method have broad-spectrum antibacterial activity, and the toughness and the oxygen barrier capacity of the edible films can be improved.

The invention provides injectable composite antibacterial hydrogel and a preparation method thereof. The method comprises the steps that in the presence of a catalyst, a carboxylated polyvinyl alcoholsolution undergoes a reaction with epsilon-polylysine after the pH value is adjusted to 3-4 so that an amino-modified polyvinyl alcohol solution is obtained, and a chitosan solution, an aldehyde dextran solution and the amino-modified polyvinyl alcohol solution are mixed to form the injectable composite antibacterial hydrogel, wherein the mass ratio of the amino-modified polyvinyl alcohol to thechitosan to the hydroformylation glucan is (4-1): 1: (0.05-0.5). According to the method, amino-modified polyvinyl alcohol with an antibacterial function is blended with chitosan, and then a dynamic Schiff base bond is constructed by utilizing amino groups on molecular chains of the polyvinyl alcohol and the chitosan and aldehyde groups on molecular chains of hydroformylation glucan, so that the hydrogel has reversible cross-linking property and is an intrinsic antibacterial material. The antibacterial rate is higher. The highest antibacterial rate of the hydrogel prepared by the embodiment ofthe invention on escherichia coli and staphylococcus aureus reaches 99.99%.

The invention relates to a carrier for improving bioavailability of proteins and water solubility of insoluble drugs and a preparation method and belongs to a carrier for constructing combined loading of a protein drug and a lyophobic drug and a preparation method. The preparation method comprises synthesis of a polyethylene glycol-poly benzyl l-glutamate block copolymer, synthesis of an ammonia product, synthesis of a linear polyethylene glycol-brushed polycarbobenzoxy lysine block copolymer and synthesis of a linear polyethylene glycol-brushed polycarbobenzoxy lysine and polylysine block copolymer. The carrier provided by the invention has the advantages that the problem of bioavailability of the protein drug and the water solubility of insoluble drugs is solved, so that various injections can be prepared; the carrier has the characteristics of improving the bioavailability of the protein drug, increasing the water solubility of the lyophobic drug, reducing the injection frequency, reducing the economical burden of a clinical patient and enhancing the drug effect.

The invention discloses an equus oocyte vitrification cryopreservation fluid, a cryopreservation method and an application. The equus oocyte vitrification cryopreservation fluid comprises a base solution (BS), an equilibrium solution (ES) and a vitrification solution (VS), wherein the VS is composed of the following components by volume percent: 10-20% of glycol (EG), 6-10% of dimethyl sulfoxide (DMSO), 6-10% of propylene glycol (PROH), 15-25% of inactivated fetal calf serum (NCS), 8-15% of carboxylate E-polylysine (COOL-PLL) and the balance of an HEPES solution. Each liter of the VS includes 0.3-0.8mol of saccharose. The invention has the beneficial effects that the vitrification cryopreservation fluid has low cytotoxicity, high permeability and obvious vitrification effect, meanwhile can obviously reduce the generation of ice crystals in freezing and re-warming processes, can protect cells from physical injury, can increase the survival rate of oocyte, is suitable for the vitrification cryopreservation of equus oocytes or embryos of horses, donkeys and the like, and is beneficial to the popularization and application of artificial insemination and embryo transplantation technology.

The invention discloses a method for preparing a sericin film by a laccase-TEMPO (2,2,6,6-tetramethylpiperidyl-N-oxide) process. A laccase is utilized to catalytically oxidize tyrosine residues in the sericin to generate quinone active groups so as to initiate the intermolecular crosslinking of sericin; and TEMPO and polylysine are added to oxidize serine residues in the sericin to generate formyl groups, thereby promoting the crosslinking between the sericin and polylysine and enhancing the forming effect and mold resistance of the sericin film. The method comprises the following steps: (1) carrying out catalytic oxidization on the sericin by using the laccase to initiate molecule crosslinking; (2) catalyzing the sericin to be grafted onto the polylysine by using laccase-TEMPO; (3) forming the sericin film; and (4) carrying out alcoholization after-treatment on the sericin film. Compared with the traditional chemical crosslinking process for preparing the sericin film, the method using the laccase and TEMPO has the advantages of high polylysine grafting catalysis efficiency and mild bioenzyme treatment conditions, and the sericin film has the advantages of favorable forming effect, obviously higher fracture strength and obviously higher mold resistance.

The invention relates to bioactive peptide milk substrate liposome essence water having blue opalescence and a Tyndall phenomenon. The bioactive peptide milk substrate liposome essence water consistsof the following components in parts by weight of 10-60 parts of milk substrate liposome, 0.1-1 part of bioactive peptide, 0.1-1 part of ginsenoside, 0.002-0.01 part of yeast essence, 0.001-0.01 partof alpha-bisabolol, 1-3 parts of tea polyphenols, 0.01-1 part of hyaluronic acid, 0.5-5 parts of trehalose, 0.01-3 parts of epsilon-polylysine and the balance distilled water, wherein all of the components are 100 parts. The essence water firstly adopts naturally-extracted bisabolol and polylysine united preservative, is free from chemical additives, has the effects of moistening, resisting wrinkles and whitening skin, is quick to seep, good in absorption and rich in nutrients, and can solve the problems that at current, essence water is difficult to deliver to a skin base layer or only a fewof active components can be delivered to the skin base layer for development of effects.

The invention relates to milk substrate liposome eye gel for resisting wrinkles and removing fat granules and dark eye circles. The milk substrate liposome eye gel consists of the following componentsin parts by weight of 10-60 parts of milk substrate liposome, 0.1-1 part of bioactive peptide, 0.1-1 part of ginsenoside, 0.002-0.01 part of yeast essence, 3-10 parts of sweet almond oil, 0.1-2 partsof tween 80, 0.1-2 parts of span 80, 0.2-2 parts of xanthan gum, 0.1-1 part of sodium alginate, 0.2-1 part of alpha-bisabolol, 1-3 parts of tea polyphenols, 0.1-5 parts of vitamin E, 0.01-1 part of hyaluronic acid, 1-5 parts of trehalose, 0.01-3 parts of epsilon-polylysine and the balance distilled water, wherein all of the components are 100 parts. The eye gel has the effects of lightening darkeye circles, resisting wrinkles, and removing fat granules. In addition, the bisabolol and the polylysine are united for use for the first time to replace chemical preservatives, so that stimulation effect on sensitive skin and muscle is eliminated.

The invention discloses a hyaluronic acid and epsilon-polylysine cross-linking method, an obtained composite cross-linked product and an application. The method includes subjecting hyaluronic acid orsalt thereof and epsilon-polylysine or salt thereof to a cross-linking reaction in condition of a coupling agent, an auxiliary coupling agent and a neutral or alkaline water environment to obtain thecomposite cross-linked product. According to the method, protonation of epsilon-polylysine or salt thereof is inhibited in a neutral or alkaline environment, so that carboxyl of hyaluronic acid or salt thereof and amino of epsilon-polylysine or salt thereof are subjected to amidation reaction, and flocculating settling is inhibited or avoided. The hyaluronic acid or the salt thereof is crosslinkedwith the epsilon-polylysine or the salt thereof, the epsilon-polylysine or the salt thereof is equivalent to a cross-linking agent, catabolism of the epsilon-polylysine or the salt thereof in vivo can be absorbed and utilized by a human body, the biocompatibility is good, and cytotoxicity easily caused by a traditional cross-linking agent can be avoided.

The invention discloses a medicine composition and gel for oral mucosa inflammatory pain and bacteriostasis and a preparation method. Bufalin, dyclonine, chitosan quaternary ammonium salt and epsilon-polylysine are used as the medicine composition to treat symptoms of oral ulcer and mucosa inflammatory pain, the medicine composition is a compound preparation according to the mechanism of pain on the surface of oral ulcer and pain causing base of inhibiting multiple microorganisms on the surface of the ulcer, the bufalin and the dyclonine are combined to quickly block pain conduction of exposednerve endings for a long time, food addition of non-western medicine antibiotics and combination of chitosan quaternary ammonium salt and epsilon-polylysine are adopted to effectively kill multiple pathogenic microorganisms on the surface of the ulcer, inflammatory irritation is further reduced, and the healing function of the wound ulcer is promoted.

The invention discloses an HA (hyaluronic acid)-mediated targeted double-drug-loading cationic liposome coating, which is a cationic liposome coating co-modified by HA (hyaluronic acid), TPGS (tocopheryl phosphate synthase) and polylysine-deoxycholic acid (PLL-DA), and is used for co-delivering PTX (paclitaxel) and SOR (sorafenib) so as to treat multidrug resistance cancers. And the liposome coating adopts a lipid membrane hydration-ultrasonic method to prepare the drug-loaded TPD-CL-PTX / SOR liposome of the HA coating. The multifunctional liposome (HA-TPD-CL-PTX / SOR) can be preferentially gathered at a tumor part through a passive targeting effect and a CD44 mediated active targeting effect after intravenous injection. PTX and SOR are released from tumor cells and have a synergistic anti-tumor effect. Besides, TPGS can further interfere with the mitochondrial function and prevent energy supply of a P-gp efflux pump so as to reduce efflux of PTX or SOR to the maximum extent, so that drugs with high treatment concentration are maintained in cancer cells, multi-drug resistance is effectively reversed, and the method is promising.

The invention discloses a method for prolonging the fresh-keeping period of fresh Chinese wolfberries, which comprises the following steps of: spraying a nutritional preservative consisting of 1-methylcyclopropene, marine fish protein peptide and epsilon-polylysine before picking, loading the Chinese wolfberries into a portable controlled atmosphere box on the same day after picking the next day, transporting the Chinese wolfberries to a destination within 12-16 hours, pre-cooling the Chinese wolfberries in a refrigeration house, storing the Chinese wolfberries in the refrigeration house, adding a terahertz fresh-keeping bag and a 1-MCP fresh-keeping bag, and adding a proper amount of dry ice, covering and storing. The fresh-keeping method can effectively slow down softening and rotting of the fresh Chinese wolfberry fruits, meanwhile, it is guaranteed that leaves of the Chinese wolfberry fruits are bright green, and the fresh-keeping period is prolonged to 50 d or above.

The invention discloses a size-controllable sodium alginate / polylysine self-assembled fiber, and belongs to the field of natural high-molecular polymeric fibers. A thin polymer film is formed in an interface between a sodium alginate solution and a polylysine solution through the static self-assembly effect, and the thin film is continuously polymerized into fibers in the interface under pulling of external face. A method is environmentally friendly and simple, the cost is low, the production efficiency is high, the requirement for instrument equipment is low, and the size-controllable sodiumalginate / polylysine self-assembled fiber is suitable for industrial production; and the diameter of the sodium alginate / polylysine fiber is in direct proportion to the area of the contact interface ofthe two solutions, and the size-controllable sodium alginate / polylysine self-assembled fiber is good in flexibility, high in filamentation rate, high in stretching resistance and suitable for the fields such as textile industry, foods, medical treatment, antibacterial materials and environmental adsorption materials.

The invention discloses a Kartogenin cartilage delivery material based on epsilon-polylysine and a preparation method thereof material. The cartilage delivery material is prepared by connecting Kartogenin molecules and epsilon-polylysine through covalent bonds. According to the cartilage delivery material, the residence time of KGN in an articular cavity can be prolonged, and the solubility of theKGN is improved; meanwhile, the material has cartilage permeability and cell membrane permeability, permeates into cartilage tissues and stem cells and cartilage cells in the cartilage tissues in anintegral material form, and is degraded under the action of intracellular enzymes to release the KGN, so that the bioavailability of the KGN is improved; and the tissue and cell compatibility is excellent, and the toxic and side effects cannot be caused. The invention also discloses a kit, freeze-dried powder of the Kartogenin cartilage delivery material is used as a component A, a biocompatible solvent is used as a component B, the use process is very simple, and the component A and the component B can be directly subjected to articular cavity injection administration after being mixed and dissolved.

The invention discloses the technical field of wet tissue preparation, and particularly relates to an antibacterial and antiviral wet tissue and a preparation method thereof. A silver ion antibacterial agent is prepared in the mode that metal silver simple substance with the particle size reaching the nanoscale is adopted, and silver ions are put into a carrier to be made into a solution, the silver ions are subjected to a contact reaction, consensus components of microorganisms are damaged or dysfunction is generated, when a trace amount of silver ions reach a microbial cell membrane, the microbe carries negative charges, the microbe and the silver ions are firmly adsorbed by coulomb attraction, and the silver ions penetrate cell walls to enter cells, so that proteins are solidified, the activity of cell synthetase is destroyed, and the cells lose division and multiplication capacities to die; the silver ion antibacterial agent and the composite double-chain quaternary ammonium salt disinfectant are matched for use, so that the disinfection component has antibacterial and antiviral capabilities at the same time; and the biological peptide antibacterial gel and the polylysine antibacterial agent are matched for use, the synergistic antibacterial and antiviral effects are achieved, and the polylysine antibacterial agent has a certain preservative effect, is a natural preservative and can enable the disinfection component to keep the disinfection activity for a long time.

The invention discloses a novel anti-cancer polymer drug. A preparation method of the drug comprises the following steps: polymerizing pyrrolidone by utilizing an atom transfer radical polymerization method, thus obtaining polypyrrolidone; modifying polypyrrolidone by utilizing folic acid molecules; polymerizing lysine benzyl ester carboxylic acid anhydride by utilizing a ring opening polymerization method, thus obtaining polylysine benzyl ester; grafting adriamycin onto polylysine benzyl ester via hydrazone bonds; connecting polypyrrolidone with polylysine benzyl ester with bonds through click chemistry, thus obtaining block copolymers; after dissolving the block copolymers in tetrahydrofuran respectively, transferring the solutions into a dialysis bag, dialyzing the solutions with pure water, and filtering the dialysate with a filter membrane; freeze-drying the solutions after filtration, thus obtaining a drug-loading micelle. The drug carrier micelle has a core / shell double-layer structure, wherein the outer layer is formed by hydrophilic polypyrrolidone, and the inner layer is a drug molecule coated layer. The material has the advantages that the material belongs to nanoparticles; the drug can achieve targeting delivery to cancer cells and pH-sensitive release in the cancer cells; the material has high drug loading capacity and good stability; the toxic and side effects of the drug on normal tissues and organs can be effectively reduced via the targeting function of the drug.

Resin particles include mother particles containing a biodegradable resin, and a coating layer containing at least one selected from the group consisting of a quaternary ammonium salt-containing polymer, polyacrylamide, polyvinylpyrrolidone, and polylysine on a surface of the mother particles.

The present invention relates to a wound dressing comprising hyaluronic acid-calcium and polylysine, and a manufacturing method therefor, the method comprising: (1) a step for adjusting each of the pH values of a hyaluronic acid-calcium salt and a polylysine aqueous solution to 8.4 or higher, and then mixing the hyaluronic acid-calcium salt and the polylysine aqueous solution to obtain a mixture liquid; and (2) obtaining a wound dressing from the mixture liquid obtained in Step (1).

The invention discloses a size-controllable sodium alginate / polylysine self-assembled fiber, which belongs to the field of natural high molecular polymer fibers. The invention uses electrostatic self-assembly to form a polymer film in the interface of sodium alginate solution and polylysine solution, and the film is continuously aggregated in the interface to form fibers under the traction of external force. The method of the invention is environmentally friendly, simple in method, low in cost, high in production efficiency, has low requirements on instruments and equipment, and is suitable for industrialized production. The diameter of sodium alginate / polylysine fiber is proportional to the contact interface area of ​​the two solutions. It has good flexibility, high filamentation rate and strong tensile resistance. It is suitable for textile industry, food, medical treatment, antibacterial materials and Environmental adsorption materials and other fields.

The invention relates to a gum base composition for protecting the balance of oral flora. The gum-based composition is prepared from a gum base, bioactivity mineral powder and polylysine, wherein a weight proportion of the bioactivity mineral powder and the polylysine is (2 to 25) : (0.0003 to 10). According to the gum base composition disclosed by the invention, the bioactivity mineral powder and the polylysine in the gum base composition are capable of inhibiting and killing various pathogenic bacteria in an oral cavity, preventing and treating oral problems of oral infection, oral ulcer, chronic gingisitis and the like, and have long-acting bacteria resistance; meanwhile, the bioactivity mineral powder and the polylysine have a certain selective protection function on beneficial bacteria in the oral cavity, the balance of flora in the oral cavity can be effectively regulated, and normal sanitation and health of the oral cavity can be maintained. Through remineralization of the bioactivity mineral powder on teeth, the gum base composition is capable of effectively repairing dentin defects; the gum base composition provided by the invention is convenient to use, wide in receiver groups and very strong in market application value.

The invention relates to a gum base composition for protecting the balance of oral flora. The gum-based composition is prepared from a gum base, bioactivity mineral powder and polylysine, wherein a weight proportion of the bioactivity mineral powder and the polylysine is (2 to 25) : (0.0003 to 10). According to the gum base composition disclosed by the invention, the bioactivity mineral powder and the polylysine in the gum base composition are capable of inhibiting and killing various pathogenic bacteria in an oral cavity, preventing and treating oral problems of oral infection, oral ulcer, chronic gingisitis and the like, and have long-acting bacteria resistance; meanwhile, the bioactivity mineral powder and the polylysine have a certain selective protection function on beneficial bacteria in the oral cavity, the balance of flora in the oral cavity can be effectively regulated, and normal sanitation and health of the oral cavity can be maintained. Through remineralization of the bioactivity mineral powder on teeth, the gum base composition is capable of effectively repairing dentin defects; the gum base composition provided by the invention is convenient to use, wide in receiver groups and very strong in market application value.

The invention provides an injectable composite antibacterial hydrogel and a preparation method thereof. The method comprises: adjusting the pH value of a carboxylated polyvinyl alcohol solution to 3-4 in the presence of a catalyst, and then reacting with ε-polylysine. Amino-modified polyvinyl alcohol solution is obtained; chitosan solution, glucan solution and the amino-modified polyvinyl alcohol solution are blended to obtain an injectable composite antibacterial hydrogel; amino-modified polyvinyl alcohol , The mass ratio of chitosan and glucan is 4-1:1:0.05-0.5. The method adopts amino-modified polyvinyl alcohol with antibacterial function, and after blending with chitosan, utilizes the amino group on the molecular chain of the two and the aldehyde group on the molecular chain of aldodextran to construct a dynamic Schiff base bond, so that the water The gel has reversible crosslinkability and is an intrinsically antibacterial material. It also has a high antibacterial rate. The highest bacteriostatic rate of the hydrogel prepared in the embodiment of the present invention to Escherichia coli and Staphylococcus aureus both reached 99.99%.

The invention discloses a combined germicide containing benziothiazolinone and polylysine. The combined germicide contains benziothiazolinone and polylysine which serve as effective ingredients as well as auxiliary ingredients, wherein a mass ratio of benziothiazolinone to polylysine is 1-80:1-80. The combined germicide can be applied to control for bacterial diseases of fruit trees, vegetables and cereal crops, has obvious synergistic interaction, delays drug resistance of pathogenic bacteria and has a long lasting period, and application times and consumption of pesticides are reduced, and the application cost is reduced. The combined germicide can be used for controlling diseases such as citrus canker, mango bacterial angular leaf spot disease, kiwifruit canker, cucumber bacterial angular leaf spot and the like.

The invention discloses a mildew-proof method for dried tangerine or orange peel. The method comprises the steps of material selection, lactic acid bacillus fermentation liquor preparation, polylysinesolution preparation, soaking, drying and the like. The lactic acid bacillus fermentation liquor preparation comprises three steps of fermentation culture medium preparation, inoculation culture andfiltration. A lactic acid bacillus fermentation liquor and a polylysine solution are mixed for use, so that growth of moulds and various bacteria can be effectively inhibited, the activity of polyphenol oxidase and peroxidase can be reduced, and the aims of preventing the dried tangerine or orange peel from mildewing and enhancing the stability of the dried tangerine or orange peel in a storage period are fulfilled; and the treated dried tangerine or orange peel can effectively maintain the quality of the dried tangerine or orange peel, and can still prevent the dried tangerine or orange peelfrom mildewing in an aging process in the southern humid and hot environment.

The invention discloses a rancidity prevention method for walnut kernels. The method comprises the following steps of: (1) preparing two solutions, wherein the solution A is an aqueous solution containing 0.1-0.2% by mass of sodium ascorbate, 0.05-0.1% by mass of citric acid and 0.02-0.04% by mass of epsilon-polylysine hydrochloride, and the solution B is an edible alcohol solution containing 0.05-0.1% by mass of BHT; (2) preparing a mixed solution, namely uniformly mixing 25-30% by volume of the solution A and 70-75% by volume of the solution B to obtain the mixed solution; (3) immersing the walnut kernels in the mixed solution, putting the walnut kernels in a vacuum container, and vacuumizing to remove gas containing active oxygen in the walnut kernel tissues; (4) opening a vacuum valve, enabling the vacuum container to be communicated with the outside, immersing the walnut kernels in the mixed solution, and maintaining for 10-15 minutes; and (5) fishing out the walnut kernels, drying, filling nitrogen, and packaging, thereby obtaining the product. The quality guarantee period of the walnut kernels treated by the method disclosed by the invention can reach 8-12 months at room temperature.

The invention belongs to the technical field of microorganisms, and in particular relates to a symbiotic Bacillus pumilus RFKF6 strain of red heart kiwifruit and an application thereof. The deposit number of the Bacillus pumilus RFKF6 strain is CGMCC No.14873. The Bacillus pumilus RFKF6 strain has significant bacteriostatic activity, can produce highly active pectinase and ε-polylysine, and can be applied to the preparation of bacteriostatic agents, pectinase and ε-polylysine, It can also be used in industrial production in the form of viable bacteria instead of bacteriostatic agents, pectinase and ε-polylysine.

The invention discloses kiwi fruit leaf health beverage and a preparation method thereof and belongs to the field of health beverage. The kiwi fruit leaf health beverage is solid beverage and liquid beverage and is prepared from main materials of kiwi fruit leaf extractive, folium mori extractive, common lophatherum herb extractive, rhizoma polygonati odorati extractive and flos chrysanthemi extractive and auxiliary materials of newtol, taurine, citric acid and E-polylysine. The preparation method of the liquid beverage comprises the following steps of evenly mixing the main materials and the auxiliary materials, adding deionized water according to a solid-to-liquid ratio of 6 to 1000, fully stirring, completely dissolving the materials, evenly mixing, filtering, sterilizing and filling. The preparation method of the solid beverage comprises the following steps of evenly mixing the main materials and the auxiliary materials, adding dextrin of 5% of the total weight of the main materials and the auxiliary materials, granulating and packaging. According to the kiwi fruit leaf health beverage, effect gain and complementation among the ingredients are achieved, so that the effects of clearing heat, removing toxicity, nourishing yin, moistening dryness, relieving cough, eliminating phlegm, improving eyesight, clearing heart fire, relieving restlessness, quenching thirst, reducing hypertension, hyperlipidemia and hyperglycemia and improving human body immunity are enhanced.

An alginate-e-polylysine microcapsule for embedding bioactive substances and its preparation method are disclosed. The microcapsule is a sphere with particle size of 100-1000 µm, which comprises microcapsule film and inner core. The microcapsule film is a polyelectrolyte complex hydrogel film obtained by reacting alginate withe-polylysine via static complexation. The inner core is a fluid- or hydrogel-environment comprising bioactive substances. The microcapsule is used as the carrier for cell transplantation, cell culture or embedding bioactive substances such as protein, polypeptide, enzyme or nucleic acid.