A kind of combined fungicide containing thiamethoxam and polylysine
A technology of polylysine and thiamethoxam, which is applied in biocides, animal repellents, plant growth regulators, etc., can solve problems such as unsatisfactory control effects, difficult disease control, and increased resistance of pathogenic bacteria. Obvious synergistic effect, less dosage, less resistance effect
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Embodiment 1
[0041] Example 1 Indoor Toxicity Determination of Thiplethidin and Polylysine Compounded to Citrus Canker
[0042] Determination method of indoor antibacterial effect: The growth rate method is used to determine the inhibitory effect of the fungicidal composition on the pathogen of citrus canker. Take mycelia pieces with a diameter of 5 mm from the edge of the citrus canker colony cultured on PDA medium for 4 days and inoculate them on PDA medium plates containing different concentrations of fungicides, and culture them at a constant temperature at 28 ° C. The plate without the agent was used as a blank control After 4 days, the colony diameter of each treatment was measured, and the percentage of inhibition of the growth of the mycelia was calculated. Through the regression analysis of the logarithm of each treatment dose and the probability value of mycelial growth inhibition rate, the inhibitory medium concentration (EC) of the treatment fungicide to citrus canker was calcu...
Embodiment 2
[0052] Example 2 Indoor Toxicity Determination of Mango Bacterial Keratosis in Combination of Thiemadone and Polylysine
[0053] Determination method of indoor antibacterial effect: The growth rate method is used to determine the inhibitory effect of the fungicidal composition on mango bacterial angular spot. Take mycelium pieces with a diameter of 5 mm from the edge of the mango bacterial angular spot cultured on the PDA medium for 4 days and inoculate them on the PDA medium plates containing different concentrations of fungicides, and culture them at a constant temperature at 28°C. As a blank control, measure the colony diameter of each treatment after 4 days, and calculate the inhibitory percentage of the medicament to mycelial growth. Through the regression analysis of the logarithm of each treatment dose and the probability value of mycelial growth inhibition rate, the inhibitory concentration (EC) of the treatment fungicide on mango bacterial angular spot was calculated....
Embodiment 3
[0062] Example 3 Indoor Toxicity Determination of Thiplethidin and Polylysine Compounded on Kiwifruit Canker
[0063] Determination method of indoor antibacterial effect: The growth rate method is used to determine the inhibitory effect of the bactericidal composition on kiwifruit canker bacterium. Take 5 mm mycelium pieces from the edge of the kiwifruit canker colonies cultured on PDA medium for 4 days and inoculate them on PDA medium plates containing different concentrations of fungicides, culture at a constant temperature at 28°C, and use the plate without the agent as a blank control After 4 days, the colony diameter of each treatment was measured, and the percentage of inhibition of the growth of the mycelia was calculated. Through the regression analysis of the logarithm of each treatment dose and the probability value of the mycelial growth inhibition rate, the inhibitory medium concentration (EC) of the treatment fungicide to kiwifruit canker was calculated. 50 ), an...
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