Method for preparing sericin film by laccase-TEMPO (2,2,6,6-tetramethylpiperidyl-N-oxide) process
A technology for sericin and sericin, which is applied in the field of preparing sericin membrane by laccase-TEMPO method, can solve the problems of decreased biocompatibility of sericin materials, and achieves ease of treatment conditions, high catalytic efficiency, and safe treatment process. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0019] Using the sericin obtained after the degumming of mulberry silk by the soda ash method, the sericin film is prepared by the method mentioned in the present invention, and the specific process steps are as follows:
[0020] (1) Laccase-catalyzed oxidation of sericin molecular cross-linking: add laccase to sericin solution, wherein sericin 20g / L, laccase 5U / mL, treatment temperature 45°C, pH 5, time 2 hours;
[0021] (2) Laccase-TEMPO catalyzed sericin grafted polylysine: add TEMPO to 10g / L to the system in step (1), ε-polylysine (molecular weight 3000) 5g / L, at 45°C, pH 5 conditions for 12 hours;
[0022] (3) Formation of sericin film: take the solution after the reaction in step (2), spread it flat in the polytetrafluoroethylene mold by spreading, and air-dry at 30°C to form a film;
[0023] (4) Post-alcoholization treatment of sericin film: immerse the sericin film treated in step (3) in 60% (v / v) ethanol solution, treat it at 25°C for 0.5 hour, and then bake it at 30...
Embodiment 2
[0029] Using sericin obtained after degumming silkworm silk at 120°C, a sericin film is prepared by the method mentioned in the present invention, and the specific process steps are as follows:
[0030] (1) Laccase-catalyzed oxidation of sericin molecular cross-linking: add laccase to sericin solution, wherein sericin 25g / L, laccase 15U / mL, treatment temperature 50°C, pH 5.5, time 2 hours;
[0031] (2) Laccase-TEMPO catalyzed sericin grafting polylysine: add TEMPO to the system in step (1) to 12.5g / L, ε-polylysine (molecular weight 3600) 7.5g / L, at 50°C , treated at pH 5.5 for 10 hours;
[0032] (3) Formation of sericin film: take the solution after the reaction in step (2), spread it flat in the polytetrafluoroethylene mold by flow extension, and vacuum freeze-dry at -50°C to form a film;
[0033] (4) Post-alcoholization treatment of sericin film: immerse the sericin film treated in step (3) in 65% (v / v) ethanol solution, alcoholize it at 25°C for 1 hour, and then heat it un...
PUM
Property | Measurement | Unit |
---|---|---|
Breaking strength | aaaaa | aaaaa |
Breaking strength | aaaaa | aaaaa |
Breaking strength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com