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A kind of method for preparing sericin film by laccase-tempo method

A technology of sericin and sericin, which is applied in the field of preparation of sericin film by laccase-TEMPO method, can solve the problems such as the decline of biocompatibility of sericin material, achieve ease of treatment conditions, avoid environmental pollution, and high catalytic efficiency Effect

Active Publication Date: 2019-01-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the chemical cross-linking method is more efficient and can effectively improve the formability of sericin, but there are also some shortcomings, such as the residue of some cross-linking agents will reduce the biocompatibility of sericin materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Using the sericin obtained after the degumming of mulberry silk by the soda ash method, the sericin film is prepared by the method mentioned in the present invention, and the specific process steps are as follows:

[0020] (1) Laccase-catalyzed oxidation of sericin molecular cross-linking: add laccase to sericin solution, wherein sericin 20g / L, laccase 5U / mL, treatment temperature 45°C, pH 5, time 2 hours;

[0021] (2) Laccase-TEMPO catalyzed sericin grafted polylysine: add TEMPO to 10g / L to the system in step (1), ε-polylysine (molecular weight 3000) 5g / L, at 45°C, pH 5 conditions for 12 hours;

[0022] (3) Formation of sericin film: take the solution after the reaction in step (2), spread it flat in the polytetrafluoroethylene mold by spreading, and air-dry at 30°C to form a film;

[0023] (4) Post-alcoholization treatment of sericin film: immerse the sericin film treated in step (3) in 60% (v / v) ethanol solution, treat it at 25°C for 0.5 hour, and then bake it at 30...

Embodiment 2

[0029] Using sericin obtained after degumming silkworm silk at 120°C, a sericin film is prepared by the method mentioned in the present invention, and the specific process steps are as follows:

[0030] (1) Laccase-catalyzed oxidation of sericin molecular cross-linking: add laccase to sericin solution, wherein sericin 25g / L, laccase 15U / mL, treatment temperature 50°C, pH 5.5, time 2 hours;

[0031] (2) Laccase-TEMPO catalyzed sericin grafting polylysine: add TEMPO to the system in step (1) to 12.5g / L, ε-polylysine (molecular weight 3600) 7.5g / L, at 50°C , treated at pH 5.5 for 10 hours;

[0032] (3) Formation of sericin film: take the solution after the reaction in step (2), spread it flat in the polytetrafluoroethylene mold by flow extension, and vacuum freeze-dry at -50°C to form a film;

[0033] (4) Post-alcoholization treatment of sericin film: immerse the sericin film treated in step (3) in 65% (v / v) ethanol solution, alcoholize it at 25°C for 1 hour, and then heat it un...

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Abstract

The invention discloses a method for preparing a sericin film by a laccase-TEMPO (2,2,6,6-tetramethylpiperidyl-N-oxide) process. A laccase is utilized to catalytically oxidize tyrosine residues in the sericin to generate quinone active groups so as to initiate the intermolecular crosslinking of sericin; and TEMPO and polylysine are added to oxidize serine residues in the sericin to generate formyl groups, thereby promoting the crosslinking between the sericin and polylysine and enhancing the forming effect and mold resistance of the sericin film. The method comprises the following steps: (1) carrying out catalytic oxidization on the sericin by using the laccase to initiate molecule crosslinking; (2) catalyzing the sericin to be grafted onto the polylysine by using laccase-TEMPO; (3) forming the sericin film; and (4) carrying out alcoholization after-treatment on the sericin film. Compared with the traditional chemical crosslinking process for preparing the sericin film, the method using the laccase and TEMPO has the advantages of high polylysine grafting catalysis efficiency and mild bioenzyme treatment conditions, and the sericin film has the advantages of favorable forming effect, obviously higher fracture strength and obviously higher mold resistance.

Description

technical field [0001] The invention relates to a method for preparing a sericin protein film by a laccase-TEMPO method, in particular to a method for catalyzing and oxidizing tyrosine residues in sericin with laccase to trigger intermolecular crosslinking of sericin, and then adding 2,2 , 6,6-tetramethylpiperidine-nitrogen-oxide (TEMPO) and polylysine, by oxidizing serine residues in sericin, promote the cross-linking of sericin and polylysine, and improve the sericin film The molding effect and antibacterial performance belong to the field of textile biotechnology. Background technique [0002] Silk is composed of silk fibroin and sericin covering the surface of silk fibroin. Among them, silk fibroin has a linear structure and contains more non-polar amino acids; sericin is a spherical protein with more hydrophilic amino acids, mainly random coils, almost no α-helical structure, and a relatively loose molecular structure. During the silk degumming process, sericin dissol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/12C08J5/18C08L89/00C08H1/00
CPCC08H1/00C08J5/18C08J7/12C08J2389/00
Inventor 王平张谦季吉王强范雪荣
Owner JIANGNAN UNIV
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