46 results about "Dunaliella sp." patented technology

The invention discloses and provides a leaf surface resistance and control agent for blocking heavy metal accumulation and a preparation method thereof. The leaf surface resistance and control agent is prepared from the following components in parts by weight: 1-3 parts of borax, 5-9 parts of nano silicon dioxide, 10-17 parts of carbon nano tubes, 5-8 parts of a surfactant, 18-31 parts of tea seedcake, 7-9 parts of dunaliella, 13-22 parts of flammulina velutipes fungus chaff, 3-5 parts of betel nut tree sawdust, 9-13 parts of a microbial agent, 3-4.5 parts of orange peel powder, 8-13 parts ofdistillers' grains, 7-10 parts of artemisia apiacea leaves, 25-43 parts of tea saponin, 1-3 parts of citric acid, 12-28 parts of amino acids, 6-11 parts of humic acid and 1000-1500 parts of deionizedwater. The leaf surface resistance and control agent prepared from the raw materials can prevent plants from absorbing heavy metals in air and soil and prevent the heavy metals from damaging the plants. Meanwhile, according to the preparation method of the leaf surface resistance and control agent for blocking heavy metal accumulation, effective components in the raw materials can be fully utilized, and the method is easy to operate and beneficial to industrial promotion.

The invention discloses an anti-aging essence for skincare products and an anti-aging skincare product using the anti-aging essence and relates to the field of cosmetics. The anti-aging essence comprises Anigozanthos manglesii extract, palmitoyl tripeptide-5 and Dunaliella salina extract, wherein the weight percentage of the Anigozanthos manglesii extract in the whole skincare product system is 0.002-0.05%, the weight percentage of the palmitoyl tripeptide-5 in the whole skincare product system is 0.0005-0.0175%, and the weight percentage of the Dunaliella salina extract in the whole skincare product system is 0.0025-0.0875%. The anti-aging essence is good in anti-aging effect and capable of reducing and relieving deep wrinkles, eliminating fine wrinkles and skin darkness and yellowness, brightening skin color and improving the early aging and aging states of the skin.

The invention discloses a cosmetic composition with the long-lasting soothing and repairing effect and application thereof. The cosmetic composition comprises 0.5-5% of panthenol, 0.25-2.5% of myrothamnus flabellifolia leaf / stem extract, 0.01-0.1% of glycerophosphoinositide choline salt and 0.01-0.1% of dunaliella salina extract. Glyceryl glucoside is an active ingredient of the myrothamnus flabellifolia leaf / stem extract, and the myrothamnus flabellifolia leaf / stem extract is an enantiomer of the glyceryl glucoside. The cosmetic composition containing the panthenol, the myrothamnus flabellifolia leaf / stem extract, the glycerophosphoinositide choline salt and the dunaliella salina extract can immediately soothe skin allergic reactions, improve skin tolerance and reduce skin redness causedby irritation, and is mild and safe. The composition with the soothing and repairing effect can relieve skin's lipid metabolism imbalance caused by irritation when being added into cosmetics, therebybeing beneficial to recovery of essential functions. The skin's barrier function can be recovered, the entry of irritants into deep layers of the skin is reduced, moisture volatilization is decreased,and the skin is restored to a healthy state.

The invention relates to a mutation breeding method for high lipid dunaliella, in particular to a mutation breeding method to obtain high lipid content dunaliella through ultraviolet mutation and extraction and screening by ether and other reagent, which belongs to dunaliella field; wherein, the obtained high lipid dunaliella is Danaliella bardawil var. HL. The invention is characterized in that: sterilized culture medium is cooled to inoculate dunaliella, and a dunaliella solution is obtained; the dunaliella solution is light-cultured, and the OD630 value is measured; parent strain is coated on the culture medium, and is cultured after ultraviolet radiation mutation; single colonies after mutation treatment is selected and cultured; the culture medium is subject to centrifugal elutriation, and a fresh dunaliella is obtained; the fresh dunaliella is collected and extracted, and the extract is dried and weighted; a mutational strain is selected, and is inoculated with the parent strain for culture; dunaliella cell is collected through centrifugation, and is dried into dunaliella powder; the total lipid content of the dunaliella powder is measured, and is determined higher than that of the mutational strain of the parent strain; lipid in the dunaliella powder of the high lipid mutational strain is subject to secondary screening, and the mutational train is verified to be a high lipid dunaliella mutational train.

A medical cold compress mask comprises a base layer, a gel layer and an isolation layer. The gel layer is positioned between the base layer and the isolation layer and comprises, preferably, 0.5-7 parts of magnesium sulfate, 0.05-3.5 parts of bisabolol, 0.05-3 parts of chitosan, 0.01-2.3 parts of sodium hyaluronate, 0.5-2 parts of tremella polysaccharides, 0.5-5 parts of spirulina polypeptide, 0.3-3 parts of hydrogenated castor oil, 0.2-1.5 parts of hydroxyethyl cellulose, 0.1-1.3 parts of allantoin, 0.2-1.8 parts of fourstamen stephania root extract, 2-8 parts of glycerol, and 60-90 parts ofpurified water. The chitosan herein is preferably carboxymethyl chitosan; the spirulina polypeptide is preferably Dunaliella salina extract. The main ingredients of the medical cold compress mask haveclear acting mechanisms and precise composition; the medical cold compress mask can remove swelling and blood stasis quickly, can inhibit bacteria and inflammations, can heal postoperative injured skin, and also has the effects of tonifying water and preserving moisture, removing acnes and fading speckles, whitening skin, and treating subhealthy skin; a production technique is simple and is easyto industrially popularize and apply.

The invention relates to Dunaliella gene engineering, in particular to a Dunaliella lycopene-beta-cyclase (LycB) gene knockout vector and application thereof. The LycB gene knockout vector comprises a selection marker gene and is connected to a 3' end homologous recombination region and a 5' homologous recombination region of the LycB gene. The LycB gene knockout vector is applied to culturing LycB gene knockout Dunaliella. The process of biologically synthesizing beta-carotene by using LycB gene knockout Dunaliella is stopped at the lycopene stage; and under the stress condition, high-content lycopene can be accumulated and the accumulation quantity is 3 to 4 percent of the dry weight of the Dunaliella.

The invention relates to a method for biologically synthesizing lycopene, in particular to a method for enabling Dunaliella to accumulate high-content lycopene. The method comprises the following steps of: extracting Dunaliella genome DNA; cloning a lycopene-beta-cyclase (LycB) gene of the DNA; constructing an LycB gene knockout vector and transforming the vector into a Dunaliella salina cell; and screening and identifying to obtain LycB gene knockout Dunaliella, wherein the LycB gene expression of the Dunaliella is inhibited; the process of biologically synthesizing beta-carotene by using the cell is stopped at the lycopene stage; and under the stress condition, a large amount of lycopene can be accumulated in an organism and the lycopene content is 3 to 4 percent of the dry weight of the Dunaliella. The method is a new way of obtaining a large amount of natural lycopene with low price.

The ultraviolet ray mutagenesis, selective breeding and identification method of cold tolerant Dunaliella includes: inoculating Dunaliella to culture medium; lighting and dark inducing for synchronized growth; mixing Dunaliella liquid with iodine solution or bromophenol blue solution to deactivate Dunaliella, injecting Dunaliella liquid to culture dish, mutagenesis under ultraviolet lamp before dark culturing, mixing with fresh culture liquid and painting the mixture to Dunaliella culture medium for low temperature lighting culture; inoculating single Dunaliella colony to the culture liquid and low temperature lighting culturing; sampling detection to comparing the low temperature lighting growth curves of cold tolerant Dunaliella mutant and wild prototype strain; extracting total DNA for RAPD comparison; calculating genetic similarity coefficient; extracting total protein and post-electrophoresis staining while record results; comparing protein electropherogram; and confirming the obtained cold tolerant Dunaliella mutant.

The invention provides soil microalgae of a mining area, a separation and purification method for the soil microalgae of the mining area and application of the soil microalgae of the mining area. Thesoil microalgae of the mining area are separated and purified from biological crusts of surfaces of tailings and comprise one or more selected from Chlorella, Cylindrotheca, Diatom, Nitzschia, Schizochytrium, Dunaliella, Scenedesmus, Nannochloropsis oculata, Chlamydomonas, Tetraselmis and Eudovina. The soil microalgae of the mining area, provided by the invention, are separated and purified from the biological crusts of the surfaces of the tailings; the obtained microalgae are relatively extensive in variety; and the obtained microalgae have relatively good adsorption and absorption capabilityto contaminants such as heavy metals and phosphorus in contaminated soil and water and then can effectively treat the contaminants such as the heavy metals and phosphorus in the contaminated soil andwater, so that the treatment cost of the contaminants such as the heavy metals and phosphorus in the contaminated soil and water is reduced greatly.

The present disclosure relates to methods of transforming various species of algae, for example, algae from the genus Scenedesmus and the genus Dunaliella, vectors and nucleic acid constructs useful in conducting such transformations, and recombinant algae, for example, Scenedesmus and Dunaliella produced using the vectors and methods disclosed herein.

The invention discloses a method for increasing content of zeaxanthin in anabaena. The method is characterized by comprising the following specific steps: cloning critical rate-limiting enzyme gene beta-carotene hydroxylase gene for synthesis of zeaxanthin from dunaliella salina teodoresce, constructing the gene into a shuttle expression vector pRL25C, and transferred into anabaena by a triparetal conjugation method, genetically modified anabaena is obtained by resistance screening, and after 16000Lx high-light treatment, the method has the advantage that the content of zeaxanthin in genetically modified anabaena is increased greatly compared with wild type anabaena, and can be increased by 3.8 times to the greatest extent.

The invention belongs to the technical field of plant molecular identification, and particularly relates to a strain identification method based on a dunaliella core gene sequence. The method mainly comprises the following steps: collecting, purifying and culturing a sample; extracting the DNA of the whole genome; constructing a DNA sequencing library; obtaining whole genome sequencing data of thealgae strain to be detected and the Dunaliella Quartolecta; screening and de novo assembly of sequencing fragments of the D. Quartolecta core genome are carried out, and genome segmentation, proteinfunction annotation and genome contig colinearity analysis are carried out on the assembled core genome sequence; constructing a phylogenetic tree by utilizing single nucleotide polymorphism, and whenan algae strain to be detected and Dunaliella Quartolecta are gathered into a cluster, the data support rate of branches is 0.99-1.00, the genetic similarity percentage is greater than or equal to 99%, and the algae strain to be detected is D.quartolecta.

The invention discloses a microbial water purifying agent for grass shrimp farming wastewater and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps: adding 30-50 parts by weight of magnetic silica beads with surface modification of C8 and C18 alkyl chains into 300-500 parts by weight of water; adding 150-200 parts by weight of a microbial reagent comprising 40-70 parts of rhodomicrobium, 40-50 parts of rhodobactersphacroides, 20-40 parts of nitrosomonas, 20-30 parts of nitrobacterwinogradskyi, 10-30 parts of pseudomonasstutzeri, 20-40 parts of leptothrix, 4-5 parts of thiobacillus, 2-5 parts of beggiatoa, 50-80 parts of nematodes, 30-40 parts of aeolosomatidaes, 20-40 parts of dunaliella and 20-40 parts of chlorella; and further adding 70-90 parts by weight of a beef extract peptone agar culture medium for culture. By putting the microbial water purifying agent disclosed by the invention into a grass shrimp farming pond, the purpose of purifying the water in the farming pond can be achieved.

The invention discloses a facial cream composition containing lipidosome with an anti-aging effect and a preparation method of the facial cream composition. The facial cream composition is prepared from the following raw material components of 0.1 to 15 percent of lipidosome with an anti-aging effect, 1 to 30 percent of a humectant, 1 to 22.5 percent of an emollient, 1 to 21 percent of an emulsifying agent, 0.1 to 10 percent of a filling agent, 1 to 12.5 percent of a thickening agent, 0.1 to 3.5 percent of a preservative, 0.01 to 1 percent of a pH regulator, 0.01 to 1 percent of daily essence and the balance of de-ionized water. The main components of the lipidosome with the anti-aging effect comprise hexapeptide-11, a dunaliella salina extract, an epilobium fleischeri extract and a fiveleaf gynostemma herb extract. By compounding the components at the same time, synthesis of skin collagen can be increased, the activity of superoxide dismutase is enhanced, excessive free radicals in skin are removed, peroxidation damage of tissue or cells is relieved, skin barrier is enhanced, and skin structure is strengthened to resist skin aging and relaxation.

The invention discloses a microbial water purifying agent for weever farming wastewater and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps: adding 40-60 parts by weight of magnetic silica beads with surface modification of C8 and C18 alkyl chains into 200-300 parts by weight of water; adding 200-300 parts by weight of a microbial reagent comprising 50-80 parts of oscillatoria, 40-80 parts of rhodomicrobium, 40-60 parts of nitrosococcus, 30-50 parts of nitrospina, 30-40 parts of crenothrix, 30-40 parts of pseudomonas aeruginosa, 3-6 parts of leucothrix, 3-6 parts of beggiatoa, 40-60 parts of zooflagellates, 40-60 parts of suctorians, 40-50 parts of dunaliella and 40-50 parts of blue-green algae; and further adding 80-100 parts by weight of a beef extract peptone agar culture medium for culture. By putting the microbial water purifying agent disclosed by the invention into a weever farming pond, the purpose of purifying the water in the farming pond can be achieved.

The present invention provides transgenic plants transformed with exogenous nucleic acid encoding a Dunaliella plasma membrane (PM)-ATPase. The transgenic plant has increased tolerance to salt as compared to a corresponding non-transgenic plant. The present invention also provides nucleic acids encoding a chimeric PM-ATPase, which comprise a first portion encoding a plant PM-ATPase or a fragment thereof, and a second portion encoding a Dunaliella PM-ATPase or a fragment thereof. The present invention also discloses a method of producing a transgenic plant having an increased tolerance to salt as compared to a corresponding non-transgenic plant, a method of modifying a plant capacity to survive salt shock, and a method of modifying plant recovery after exposure to salt stress, by introducing into one or more cells of a plant an exogenous nucleic acid encoding a Dunaliella PM-ATPase. Also provided by the present invention are plant cells comprising an exogenous nucleic acid encoding a Dunaliella PM-ATPase, and plant seeds and progeny obtained from the transgenic plants.

The invention discloses a face cream composition containing lipidosome for improving skin elasticity and a preparation method of the face cream composition. The face cream comprises the following components in parts by weight: 0.1 to 16 parts of lipidosome for improving skin elasticity, 0.1 to 28 parts of an emollient, 0.12 to 14.5 parts of an emulsifier, 0.15 to 10.5 parts of a filler, 0.24 to 22.4 parts of a thickener, 0.01 to 5 parts of a preservative, 0.02 to 3 parts of EDTA-2NA, 0.015 to 0.65 part of a pH regulator, 0.02 to 0.5 part of daily essence and the balance of deionized water. The lipidosome for improving skin elasticity comprises the following main components: a fucus serratus extract, a palmitoyl tripeptide-5 / dunaliella salina / sodium hyaluronate / provitamin B5 compound and sialic acid. According to the face cream composition containing the lipidosome for improving the skin elasticity, the skin elasticity and softness can be enhanced by compounding all the components at the same time, the lipidosome is used as a delivery carrier of the functional components, the stability of the functional components can be kept, the transdermal efficiency of the functional components can be enhanced, and the capability of improving the skin elasticity for the liposome is increased.

The invention discloses a microbial water purifying agent for mandarin fish culture water and a preparation method of the agent. The preparation method comprises the following steps: adding 40-50 parts by weight of magnetic silicon spheres of which the surface is modified with C8 and C18 alkyl chains to 200-250 parts by weight of water; adding 100-150 parts by weight of microbial reagents which include 40-60 parts of synechococcus, 40-50 parts of rhodobacter sphaeroides, 30-40 parts of nitrospina, 30-50 parts of nitrococcus, 20-40 parts of sphaerotilus natans, 30-50 parts of pseudomonas aeruginosa, 3-5 parts of leucothrix, 3-5 parts of thioploca, 50-60 parts of amoeba, 30-50 parts of suctorida, 30-40 parts of dunaliella and 30-50 parts of tetraselmis; next, adding 50-80 parts by weight of beef extract peptone agar culture medium for culturing. The microbial water purifying agent is put into a mandarin fish culture pond to purify water in the culture pond.

The invention discloses a method for preparing high-value feedstuff for aquatic products by using microalgae and artemia salina. The high-value feedstuff for the aquatic products is prepared through culturing nauplii of artemia with the microalgae, and then, carrying out collecting, sterilizing, freeze-drying and crushing, wherein the microalgae comprise at least one of dunaliella salina, chlorella salina, isochrysis galbana, nannochloropsis oculata and platymonas subcordiformis. According to the method disclosed by the invention, the specific high-value microalgae serve as a unique food supply of the nauplii of artemia, a one-step food cycle culture mode is employed, the microalgae have multiple nutrients and a high value, can be used for promoting and improving the growth, development and immunity of the artemia salina and have the characteristics of high artemia salina survival rate, high nutritive value, environmental-friendliness, safety and simple culture process; and due to aftertreatment, the appearance, color and luster of the artemia salina can be completely reserved to the maximum, the damage to the nutrients is less, nutritive values for the feedstuff for the aquatic products are reserved relatively greatly, and the prepared feedstuff for the aquatic products is rich in nutrition and relatively low in cost, does not pollute culture water and has an application prospect.

The present invention provides dunaliella salina CPD photoreactivating enzyme which can rehabilitate the damage of ultraviolet radiation, a nucleotide sequence which codes the dunaliella salina CPD photoreactivating enzyme and the photoreactivating enzyme plastid which is prepared by the dunaliella salina CPD photoreactivating enzyme and can rehabilitate the damage of ultraviolet radiation,; wherein, the prepared photoreactivating enzyme plastid can be used as the raw material which is used to produce the medicine which rehabilitates the damage of ultraviolet radiation, and sun screening agent. The present invention also provides preparation methods of the dunaliella CPD photoreactivating enzyme and the photoreactivating enzyme plastid which can rehabilitate the damage of ultraviolet radiation.

The invention discloses a composition for effectively relieving skin allergy and couperose skin as well as a preparation method and application of the composition. The composition comprises a phase A, a phase B and a phase C, wherein the phase C comprises a radix gentianae albiflorae extract, a butyrospermum parkii seed cake extract, a baobab fruit extract, a coffee seed extract, a dunaliella salina extract, a pseudoalteromonas fermentation product extract, a ginseng root extract, a polygonum cuspidatum root extract, a mallow flower extract, a phellinus pharmacologically active extract, a glycyrrhiza glabra root extract, a medicinal rhizoma polygonati rhizome / root extract, a superfine angelica root extract, a fucus extract, 1, 2-pentanediol, palmitoyl hexapeptide-12 and acetyl heptapeptide-4. The invention also provides the preparation method and application of the composition. The composition is used for preparing skin care products and comprises a facial patch. The composition prepared by the invention aims at all skin types, can quickly and effectively relieve skin allergy and couperose skin, can promote skin repairing, and has moisturizing, antibacterial and anti-inflammatory functions and the like.

The invention relates to a toning lotion composition with soothing, balancing and skin care effects and a preparation method thereof. The toning lotion is prepared from the following components in percentage by weight: 1-35 percent of composition with soothing and balancing effects, 5-30 percent of humectant, 0-30 percent of skin conditioner, 0-10 percent of thickener, 0.01-2 percent of chelating agent, 0.01-1 percent of solubilizer, 0.3-5 percent of preservative, 0.01-1.0 percent of daily essence, a proper amount of pH regulator and the balance of deionized water. The functional composition is prepared from the following components: a formonella fortunei extract, a desert rose leaf cell extract and palmitoyl tripeptide-5 / dunaliella salina / sodium hyaluronate / provitamin B5. The various functional components have the effects of promoting the expression of moisturizing related factor genes, have a high moisturizing effect, balance the skin and improve the immune function of the skin and the capability of resisting external stimulation. According to the toning lotion composition disclosed by the invention, the raw materials are scientifically combined, and the synergistic effect among the functional components is utilized, so that the safe and effective moisturizing and soothing effects are finally achieved, and the skin is kept in a moisturized and comfortable state.

The present invention provides one kind of balneotherapeutic floating water and its preparation process. The balneotherapeutic floating water has Baume degree 25-32, and consists of magnesium sulfate, magnesium chloride and sodium chloride in the weight ratio of 1-0.5 to 2-0.5 to 1-0.5 except water. Its preparation process includes the following steps: filtering water or Dunalialla salina containing salt lake water with sand filter possessing felt layer and measuring the contents of magnesium sulfate, magnesium chloride and sodium chloride in the water; heating the filtered water in a pond to 20-40 deg.c; adding required magnesium sulfate, magnesium chloride and sodium chloride via stirring; and re-filtering. The balneotherapeutic floating water possesses health functions of replenishing mineral matter, assisting treatment and nursing skin.

The invention discloses an application of a compound 3,3',5,5'-tetrabromo-2,2'-biphenol in the aspect of algal inhibition. Algae removal spectrum test proves that the compound has excellent inhibitioneffect on eleven algae comprising thalassiosira pseudonana, chaetoceros mulleri, prorocentrum minimum, amphidinium carterae, nannochloropsis oculata, prochlorococcus, dunaliella salina, phaeodactylumtricornutum, chlorella vulgaris, tetraselmis subcordiformis, porphyridium cruentum, etc. The compound has great significance on treatment of red tide, algal bloom and the like natural hazard and canprevent eutrophication of water body.

The invention discloses a super-selenium-rich dunaliella salina product and a preparation method and application thereof. The preparation method of the super-selenium-rich dunaliella salina product comprises the following steps of culturing, adding inorganic selenium and acquiring a finished product, wherein the culturing specifically comprises the steps of culturing the dunaliella salina in a culture medium; addition of inorganic selenium specifically comprises the steps of adding the inorganic selenium into the culture medium; and finished product acquisition specifically comprises the steps of continuously culturing for at least 3 days after the inorganic selenium is added, collecting and drying. According to the super-selenium-rich dunaliella salina product and the preparation method and the application thereof, inorganic selenium can be converted into organic selenium by adopting dunaliella salina, and the preparation process is simple and easy to implement; the content of organic selenium in the dunaliella salina can reach 1000mg / kg or above, the content of the organic selenium is 80%-99% of the total selenium content, and the standard of super-rich selenium is met; and meanwhile, the prepared super-rich selenium dunaliella salina product can be added into food, health care products and medicines at normal temperature, and can be absorbed and utilized by digestive systems of human bodies and animals.