38 results about "ARTEMISIA ANNUA LEAF" patented technology

The invention discloses an environmentally friendly microbial pesticide for killing acarid, and the environmentally friendly microbial pesticide comprises the following components by weight: 1-3 parts of monoucleosis powder, 0.5-1.0 part of bacillus subtilis powder, 8-12 parts of herba ephedrae, 1-2.5 parts of raw ginger, 2.5-3.5 parts of erodium stephanianum willd, 20-35 parts of xanthium, 30-40 parts of pomegranate rind, 1-3 parts of mugwort leaf, 11-21 parts of cordate houttuynia, 9-16 parts of andrographis paniculata, 3-8 parts of hemp, 7-9 parts of serrate chloranthus, 9-13 parts of artemisia annua leaf, 2-5 parts of tobacco, 3-8 parts of radix phytolaccae, 7-12 parts of dandelion, 11-13 parts of leech, 8-13 parts of croton, 3-6 parts of polygonatum odoratum, 2-4 parts of nard, 1-3 parts of licorice and 3-9 parts of euphorbia kansui. The environmentally friendly microbial pesticide has characteristics of both a biological pesticide and a plant pesticide, if a synergist is added, the relationship of the pesticide efficacy and the components is geometrically progressed, the experimental data shows that the pesticide efficacy is 10-20 times of the efficacy of a single component, and the environmentally friendly microbial pesticide is low in toxicity to human and birds.

The invention provides an artemisia apiacea flour product and a production method of the artemisia apiacea product. According to the method, artemisia apiacea flour with the mass percentage being 0.5 percent to 10 percent is added, and the artemisia apiacea flour is obtained through sequentially carrying out clean washing, water removing, dewatering baking, sterilization and crushing on artemisia apiacea leaves. The production method comprises the following steps that flour, artemisia apiacea flour and auxiliary materials are uniformly mixed, warm water accounting to 30 to 55 percent of the weight of mixed raw materials with the temperature being about 30 DEG C is added, and the dough making is carried out for 15 to 20 minutes. The obtained dough is subjected to curing, tabletting, noodle cutting and drying to be made into noodles. Yeast is added into the dough, and bread is obtained through fermentation, forming and baking. The artemisia apiacea flour is added, so the bread and the noodles have the health care effect of the artemisia apiacea. Through the reasonable addition and production process, active ingredients of the artemisia apiacea are maintained to the maximum degree, meanwhile, the natural green color and the fragrance of the artemisia apiacea are remained, the mouth feeling is fresh and delicious, and the artemisia apiacea flour product belongs to rare green health care food.

The invention discloses a method for efficiently separating and instantaneously transforming protoplasts of Artemisia annua, comprising: 1) selecting young leaves of Artemisia annua and removing the lower epidermis to obtain the leaves of Artemisia annua without the epidermis; 2) removing the epidermis from the leaves Putting the leaves of Artemisia annua into the enzymolysis solution for enzymolysis to obtain a mixture of enzymolysis; 3) filtering and centrifuging the mixture of enzymolysis to obtain protoplast precipitation, and obtaining protoplasts after resuspension; 4) decomposing the protoplasts Instant transformation. The present invention establishes the method for protoplast separation and transformation in Artemisia annua for the first time, and the output of protoplast is 2.749×10 5 1 / g FW, the activity is 96%, the transformation efficiency of green fluorescent protein is 80%, and the obtained protoplasts have large yield and high activity. Moreover, the protoplasts of Artemisia annua were used as receptors for transformation, and the green fluorescent protein GFP, luciferase LUC and sea cucumber luciferase REN could be successfully expressed.

The invention discloses an observation and density measurement method for glandular hairs on Artemisia annua leaf surfaces, comprising the following steps: taking a eugonic Artemisia annua leaf, and feeding the leaf in a centrifuge tube or a microtiter plate; successively adding petroleum ether or normal hexane, absolute ethyl alcohol or carbinol and glacial acetic acid, evenly mixing, and incubating for 5-20min at room temperature; and taking out the leaf, placing the left on a glass slide, adding a drop of double distilled water, covering a cover slip slightly, compacting by a tablet clip, and observing on an optical microscope or calculating the number of the glandular hairs so as to calculate the density of the glandular hairs. The method is simple and convenient, has low requirement on equipment, greatly simplifies the preprocessing procedure of experimental materials, can complete the whole operation within half a hour, uses a little of reagent, is non-toxic, safe and environment-friendly, lowers the measurement cost and time substantially, and can be used for high pass analysis.