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Method for extracting artemisinin from sweet wormwood

A technology of artemisinin and Artemisia annua, applied in the direction of organic chemistry, etc., to achieve the effect of improving extraction rate, simple operation, and short extraction time

Inactive Publication Date: 2015-10-21
兰捷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention will aim at the defects in the existing artemisinin extraction process, and provide a method for extracting artemisinin from Artemisia annua

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting artemisinin from Artemisia annua, comprising the following steps:

[0027] (1) Dry 100kg of Artemisia annua leaves (the content of artemisinin is 0.76kg) in the sun naturally so that the water content is 5%, and then crush them to 40 mesh to obtain Artemisia annua powder;

[0028] (2) Add the above-mentioned Artemisia annua powder into an ultrasonic stirrer, then add 400L of ethanol with a concentration of 90%, start the stirrer, and stir at a speed of 500r / min for 20min;

[0029] (3) Then transfer the mixed solution to the reflux device, reflux extraction 4 times, reflux each time for 0.5h, and combine the extracts;

[0030] (4) Concentrate the above extract under reduced pressure for 2 hours at a temperature of 60°C and a pressure of 0.08Mpa to obtain a concentrated solution, then use 5% citric acid to adjust the pH value of the concentrated solution to 3, and the precipitate After filtering, dry at 60°C for 1 hour to obtain crude artemisinin;...

Embodiment 2

[0035] A method for extracting artemisinin from Artemisia annua, comprising the following steps:

[0036] (1) Dry 100kg of Artemisia annua leaves (the content of artemisinin is 0.76kg) naturally, so that the water content is 5%, and then crush to 60 mesh to obtain Artemisia annua powder;

[0037] (2) Add the above-mentioned Artemisia annua powder into an ultrasonic stirrer, then add 600L of ethanol with a concentration of 95%, start the stirrer, and stir for 30min at a speed of 500r / min;

[0038] (3) Transfer the mixed solution to the reflux device, reflux and extract 3 times, reflux for 1 hour each time, and combine the extracts;

[0039] (4) Concentrate the above extract under reduced pressure for 1.5 hours at a temperature of 70°C and a pressure of 0.08Mpa to obtain a concentrated solution, and then use 5% citric acid to adjust the pH value of the concentrated solution to 3. After the precipitate is filtered, it is dried at 70°C for 1 hour to obtain crude artemisinin;

[...

Embodiment 3

[0044] A method for extracting artemisinin from Artemisia annua, comprising the following steps:

[0045] (1) 100kg of Artemisia annua leaves (the content of artemisinin is 0.74kg) are dried naturally to make the water content 8%, and then crushed to 60 mesh to obtain Artemisia annua powder;

[0046] (2) Add the above-mentioned Artemisia annua powder into an ultrasonic stirrer, then add 500L of ethanol with a concentration of 95%, start the stirrer, and stir at a speed of 800r / min for 20min;

[0047] (3) Then transfer the mixed solution to the reflux device, reflux and extract 3 times, reflux for 0.5h each time, and combine the extracts;

[0048] (4) Concentrate the above extract under reduced pressure for 1.5 hours at a temperature of 60°C and a pressure of 0.09Mpa to obtain a concentrated solution, and then adjust the pH value of the concentrated solution to 4 with 8% citric acid. After the precipitate was filtered, it was dried at 60°C for 2 hours to obtain crude artemisin...

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Abstract

The invention discloses a method for extracting artemisinin from sweet wormwood. The method comprises the following steps: drying sweet wormwood leaves in the air, grinding the dried sweet wormwood leaves, adding 3 to 6 times of ethanol into the powder, and stirring in an ultrasonic stirrer for 20 to 30 minutes, so as to obtain a mixture liquid; putting the mixture liquid into a reflux unit for reflux extraction, so as to obtain an extracting solution; conducting vacuum concentration on the extracting solution, adjusting the pH value to be 3 to 4 with citric acid, and drying to obtain an artemisinin crude product; treating the crude product with silica-gel column chromatography, dissolving the dried solid with methanol, and carrying out recrystallization, filtering and drying to obtain high-purity artemisinin. The artemisinin extraction rate can reach 93 percent, and the purity of obtained artemisinin can reach 98.5 percent or higher. The method is simple to operate, low in energy consumption, short in extracting time, high in extracting rate, high in product purity, low in cost, and easy to realize industrial production.

Description

technical field [0001] The invention relates to the technical field of extracting effective components of Chinese herbal medicines, in particular to a method for extracting artemisinin from Artemisia annua. Background technique [0002] Artemisia apiacea (artemisia apiacea) is the aerial part of Artemisia annua L., which is a traditional Chinese medicine in my country. Artemisinin is the main active ingredient in Artemisia annua. The content of artemisinin in Artemisia annua is 5-8%. Artemisinin is a sesquiterpene lipid compound with molecular formula C 15 h 12 o 5 . Artemisinin has anti-malarial, anti-pregnancy, anti-fibrosis, anti-schistosome, anti-toxoplasma gondii, anti-arrhythmia and inhibition of tumor cytotoxicity. At present, the value of artemisinin in the prevention and treatment of malaria has been recognized and accepted by human beings. The World Health Organization has listed the compound preparation of artemisinin as the first choice drug for the preventi...

Claims

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Application Information

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IPC IPC(8): C07D493/20
CPCC07D493/20
Inventor 兰捷
Owner 兰捷
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