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Microbial water purifying agent for mandarin fish culture water and preparation method thereof

A microbial reagent, aquaculture water technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as water quality decline, yield decline, and survival rate reduction, and achieve high purification efficiency and easy precipitation.

Inactive Publication Date: 2015-01-14
SHANGHAI KUIYA ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the amount of feed input is often greater than the intake of mandarin fish, if the excess feed is not effectively treated, it will easily cause water quality to decline. If the aquaculture water is not treated, it will easily cause problems such as increased incidence of mandarin fish, decreased survival rate, and decreased production.
The conventional solution is to change the water at intervals, but on the one hand, changing the water increases production and management costs, and on the other hand, it is easy to cause discomfort symptoms of mandarin fish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Preparation of magnetic silica spheres with surface-modified C8 and C18 alkyl chains:

[0025] (1-1) Synthesis of Fe by hydrothermal method 3 o 4 Magnetic nanoparticles: 1 g of FeCl 3 ·6H 2 O, dissolved in 30ml of ethylene glycol, adding 4 grams of anhydrous sodium acetate, the reaction temperature is 200 ° C, the reaction time is 12 hours, and Fe is generated 3 o 4 magnetic nanoparticles;

[0026] (1-2) The above Fe 3 o 4 Wash the magnetic nanoparticles with water to remove water-soluble impurities, and then disperse them in 10 g of water to obtain dispersion A. Take a centrifuge tube, add 3 g of the above dispersion A, add 5 ml of 2M HCl solution and sonicate for 8 minutes, and then use a magnet to separate and remove the green color. Clear liquid, then disperse with 10ml water, collect particles with magnet, remove light green clear liquid, disperse ultrasonically with 5 ml 10wt% trisodium citrate solution, let it stand for 2 hours, re-disperse with 5 ml w...

Embodiment 2

[0033] Basically the same as in Example 1, a microbial water purifier for mandarin fish aquaculture water was prepared, the only difference being that the surface modification agent in step (1-4) was different. In Example 2, 0.3 g of dimethyloctylsilane was used as the surface modifier.

Embodiment 3

[0035] Basically the same as in Example 1, a microbial water purifier for mandarin fish aquaculture water was prepared, the only difference being that the surface modification agent in step (1-4) was different. In Example 3, 0.3 g of octadecyltriethoxysilane was used as the surface modifier.

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Abstract

The invention discloses a microbial water purifying agent for mandarin fish culture water and a preparation method of the agent. The preparation method comprises the following steps: adding 40-50 parts by weight of magnetic silicon spheres of which the surface is modified with C8 and C18 alkyl chains to 200-250 parts by weight of water; adding 100-150 parts by weight of microbial reagents which include 40-60 parts of synechococcus, 40-50 parts of rhodobacter sphaeroides, 30-40 parts of nitrospina, 30-50 parts of nitrococcus, 20-40 parts of sphaerotilus natans, 30-50 parts of pseudomonas aeruginosa, 3-5 parts of leucothrix, 3-5 parts of thioploca, 50-60 parts of amoeba, 30-50 parts of suctorida, 30-40 parts of dunaliella and 30-50 parts of tetraselmis; next, adding 50-80 parts by weight of beef extract peptone agar culture medium for culturing. The microbial water purifying agent is put into a mandarin fish culture pond to purify water in the culture pond.

Description

technical field [0001] The invention relates to a microbial water purifier and a preparation method thereof, in particular to a microbial water purifier for mandarin fish culture water and a preparation method thereof. Background technique [0002] In recent years, the rapid advancement of high-efficiency agriculture has led to the rapid development of special aquaculture. Famous, special and high-quality new aquaculture species have reached the level of large-scale production and formed their own characteristics. Mandarin fish, also known as mandarin fish and Aohua fish, belongs to the lipid family fish in taxonomy. Aohuayu is the most valuable fish among the "three flowers and five Luos". Mandarin fish has a flat body, a broad belly, a large mouth and thin scales, and a short head and tail. The body shape is black spot color, the bright color is male, and the slightly darker one is female. There are fin spines on the back of the fish. The skin of mandarin fish is relat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34C12R1/385C12R1/01C12R1/90C12R1/89
Inventor 许银亚
Owner SHANGHAI KUIYA ENVIRONMENTAL PROTECTION TECH
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