The invention relates to an anti-hair loss shampoo and a preparation method thereof. The anti-hair loss shampoo is composed of the following substances in parts by weight: disodium edetate, guar gum hydroxypropyltrimethylammonium chloride, butylene glycol , Disodium Laureth Sulfosuccinate, Cocamide MEA, Trihydroxystearin, Cetyl Alcohol, Sodium Cocamidopropionate, Cocamidopropyl Hydroxysultaine, Vitamin B Complex Raw materials, Dimethicone, Laureth‑23, Laureth‑3, Anti-dehydration Plant Extract, Phenoxyethanol, Ethylhexylglycerin, Fragrance, Citric Acid, Sodium Chloride, Deionized water. The anti-hair loss shampoo of the present invention has the effect of nourishing and repairing the hair. Long-term use can not only moisturize the cuticles, make the hair not easy to break, but also make the hair black and bright, improve the growth vitality of hair follicle cells, and promote hair regeneration. Enhance hair follicle metabolism and effectively prevent hair loss.