The invention discloses a high CO-resistant anode catalyst of direct alcohol fuel cells and a preparation and an application. The catalyst is prepared by the following steps that an organic tellurium source is dissolved in an organic solvent, the mixture is placed into a buffer solution with PH in a range from 8 to 10, and the mixture is subjected to sealing reaction at a temperature of 120 DEG C to 150 DEG C for 3 to 5 hours, when the reaction is over, the reaction solution is quenched to room temperature, two phase interface products are subjected to centrifugation, sediments are washed and dried, and tellurium dendritic crystal is obtained; and the obtained tellurium dendritic crystal is mixed with glycol and gold containing precursor, the mixture is oscillated in a sealing condition at a temperature of 60 DEG C for 3 to 5 hours, when the reaction is over, the reaction solution is subjected to centrifugation, sediments are washed and dried, and the high CO-resistant anode catalyst of direct alcohol fuel cells is obtained. According to the high CO-resistant anode catalyst of direct alcohol fuel cells and the preparation and the application, the catalyst has good catalytic activity to CO, and the stability is improved greatly; and the cost is low, the operation process is simple, the period is short, and the catalyst can be produced in batches.