180 results about "Digestants" patented technology

The invention discloses a controlled release preparation with improved performance. The controlled release preparation comprises a core containing medicament and a controlled release film covering the outside of the core and being almost insoluble in water as well as stomach and intestines digestive juice. The controlled release film comprises particulate matters of a water soluble medicinal additive, the water-soluble medicinal additive is covered by a polymer film which can be soluble in the stomach and / or intestines digestive juice but almost insoluble in water, the polymer and the medicinal additive can not produce chemical reaction or can produce chemical reaction but do not produce water-insoluble non-gaseous products and the pharmaceutically unacceptable products, and the amount of the polymer is no more 700% of that of the medicinal additive. The invention also discloses a preparation method of the controlled release preparation. The controlled release preparation has the advantages of improved medicament release reproducibility, reduced medicament release lag time, accelerated medicament release and improved bioavailability, can realize located controlled release, delayed controlled release and interval type or pulse type controlled release of the medicament in the gastrointestinal tract, and the like.

A health-care noodle for helping digestion features that its raw materials including wheat flour, cassava starch, buckwheat powder, corn powder, 4 Chinese-medicinal materials including chicken's gizzard-skin, germinated millet, yam and tuckahoe, calcium carbonate and zinc gluconate.

The invention relates to a compound acidulant which is particularly added into feed of pigs and fowls and belongs to the field of feed additive. The compound acidulant is prepared by the following steps: crushing and sifting anhydrous citric acid, fumaric acid and malic acid powder; placing white carbon black into a mixer primarily, spraying the white carbon black into the mixer by a lactic acid pump to lead the white carbon black to cover all lactic acid; adding the anhydrous citric acid, the fumaric acid, the malic acid and silica into the mixer for mixing later; at last, adding bacillus subtilis and xylo-oligosaccharide into the mixer to be mixed into the finished product. The compound acidulant contains organic acid instead of inorganic acid, and the organic acid can slow the releasing speed of the acidulant so as to enable the acidulant to better exert the function; the compound acidulant has lactic acid taste and good palatability so as to improve the utilization ratio of the feed; compared with the inorganic acidulant, the lactic acid type acidulant has more remarkable effect of lactic acidulant, can better improve the intestinal microflora because of containing beneficial bacterium stimulator, and has remarkable diarrhea-preventing effect, thereby being an ideal digestant of animals.

The invention discloses a method for determining total nitrogen and total phosphor in deposits and soil, firstly drying, crushing, screening and thoroughly mixing uniformly the fresh sample collected, and weighing a certain amount and placing in a digesting container and serving; adding digestant in the digesting container and mixing them uniformly; stopping closely with ground stopper, wrapping with gauze and placing in high pressure steam boiler and making high temperature digestion and cooling and taking out; absorbing digesting supernatant and scanning at 190nm-290nm wavelengths to determine the optimum absorption wavelength and background value; measuring light absorption value and obtaining total nitrogen; absorbing digesting supernatant, adding in color developing agent and developing color at constant volume, scanning at 650-750nm wavelengths, determining the optimum absorption wavelength, determining light absorption degree and obtaining total phosphor. And the method is especially applied to combined determination of total nitrogen and total phosphor of deposits and soils in rivers, lakes, reservoirs, seas, etc.

The invention belongs to the field of biological agents and discloses triple probiotics viable bacteria powder obtained by whole oat mixed solid-state fermentation utilizing various probiotics direct-adding reagents. The triple probiotics viable bacteria powder contains bifidobacterium lactis V9, lactobacillus plantarum P-8 and lactobacillus casei Zhang, and is prepared by taking whole oat powder as a fermentation substrate, and has excellent gastrointestinal tract digestive juice endurance capacity, is capable of improving a bacterial community structure of human intestinal tracts, capable of regulating blood lipid metabolism and capable of improving pathogenic infection resistance of intestinal tracts, and effectively prevents and relieves irritable bowel syndromes such as diarrhea, constipation, stomachache, abdominal distension.

The invention discloses an application of an adipose-derived mesenchymal stem cell exosome in preparation of medicines for obesity treatment. The adipose-derived mesenchymal stem cell exosome is prepared by a method comprising the following steps of (1) dispersing stripped adipose tissue in a digestive juice to generate a cell suspension solution; (2) centrifuging the cell suspension solution to form cell sediments, separating the sediments and washing to obtain a blood vessel matrix component containing adipose-derived mesenchymal stem cells; and (3) carrying out multiplication culture on ADSC in an in vitro culture system, collecting and concentrating supernate after multiplication culture, adding an exosome extraction reagent to the concentrated supernate to obtain the adipose-derived mesenchymal stem cell exosome. The adipose-derived mesenchymal stem cell exosome is capable of promoting white fat beige to achieve heat production-driven energy consumption, thereby resisting occurrence and development of obesity.

The invention relates to a culture method of breast cancer organoids and a co-culture method of tumor-related fibroblasts. According to the invention, a more efficient and convenient improved system for key technical links such as PDO culture, passage, maintenance and the like of tumor tissue organoids of breast cancer patients is established, operation steps and methods for establishing and passage of a PDO cell line are simplified in multiple aspects, components of a culture medium, digestive juice, a washing solution and the like are optimized, and finally, a breast cancer PDO culture system is successfully established. Compared with the prior art, the culture method has the advantages that the success rate and the passage frequency are basically consistent, but the operation is simpler, the required time is shorter, a good foundation is laid for a PDO model to have better clinical and scientific research applications in the future, and the corresponding cost is saved. The inventionalso establishes a stable co-culture system of breast cancer PDO and tumor-associated fibroblasts (CAFs), simultaneous culture, amplification and passage of PDO and CAFs can be realized, and the PDOand the CAFs do not produce obvious influence on each other.

The invention relates to a feed additive, and especially relates to a rabbit feed additive. The rabbit feed additive is composed of medicated leaven, Radish Seed, dried orange peel, Weeping Forsythia, Fructus Aurantii, Radix Codonopsis, Poria cocos, white hyacinth bean, lotus seed, coix seed, Fructus Amomi, Radix Platycodonis, Rhizoma Cimicifugae, Combined Spicebush Root, Radix Scutellariae, Cyrtomium fortunei, Amur Corktree Bark and Semen Plantaginis, wherein the medicated leaven, the Radish Seed, the dried orange peel, the Fructus Aurantii, the white hyacinth bean, the Fructus Amomi and the Combined Spicebush Root are simultaneously used for digestion helping and accumulation removing; the dried orange peel, the Weeping Forsythia, the Poria cocos, the white hyacinth bean, the coix seed, the Fructus Amomi, the Rhizoma Cimicifugae, the Radix Scutellariae, the Cyrtomium fortunei, the Amur Corktree Bark and the Semen Plantaginis are simultaneously used for dampness and heat eliminating; the medicated leaven, the dried orange peel, the Radix Codonopsis, the Poria cocos, the white hyacinth bean, the lotus seed, the coix seed, the Fructus Amomi and the Radix Platycodonis are simultaneously used for spleen invigorating and stomach nourishing; and the Combined Spicebush Root, the Cyrtomium fortunei and the Amur Corktree Bark are simultaneously used for parasite expelling, sterilizing and digestive juice secretion increasing. The interaction of the above eighteen medicines can realize digestion helping, accumulation removing and dampness and heat eliminating to prevent diarrhea, and can also realize spleen invigorating, stomach nourishing and spleen and stomach harmonizing to enhance the gastrointestinal function and improve the human immunity.

The invention belongs to the field of medicines, and relates to an application of a Chinese medicine composition in the aspect of treating the apositia. The Chinese medicine composition is particles formed by frying hawthorn and malt and comprises hawthorn, malt, Chinese yam and Indian buead and the like. The Chinese medicine composition has the functions of replenishing qi, invigorating the spleen, promoting digestion and removing humidity, has the adjuvant treatment effect on the diseases such as infantile indigestion caused by damp encumbrance of the spleen and the stomach and distending pain and the like caused by the insufficient power of the stomach and the intestine by promoting the gastrointestinal motility and secretion of gastrointestinal digestive juice, and has a good clinical effect.

The invention provides a device capable of realizing automatic simulation of gastrointestinal continuous digestion and an application method of the device. The device comprises a stomach reactor, a stirrer, a constant-temperature water circulation system, a gastrointestinal fluid conveying system, a gastric emptying control device, an automatic pH titration device, a small intestine reactor and asample receiving device. According to the application method, to-be-digested food is conveyed into a stomach digester, stomach digestive fluid is input at a constant speed, the stirrer simulates stomach peristalsis, a micropore glass plate at the bottom of the stomach reactor simulates a pylorus to realize stomach "sieving emptying", the to-be-digested food is conveyed into a pH regulation chamberfor automatic pH regulation, small intestine digestion is simulated in the small intestine reactor provided with an internally-arranged latex sleeve, and the pressure-changing water circulation system is adopted to realize regular vibration of the latex sleeve so as to simulate the stomach peristalsis. Through the device and the application method, the whole reaction process can be continuous andautomatic under the situation that parameters are set; and moreover, the device is simple in structure, free of a complicated control system and easy to assemble and can be used to realize a digestion simulation experiment in a scientific research experiment.

The invention belongs to the technical field of food biology and in particular relates to a method for preparing an antioxidant collagen peptide. The main technical method comprises the following steps: mixing collagen and artificial gastric juice (according to United States Pharmacopeia preparation) under the condition of 37 DEG C, simulating gastric digestion under oscillating conditions, regulating the pH to 6.8 by using NaOH, adding pancreatin and continuously simulating intestinal digestion, ending the reaction after a boiling water bath, centrifuging and taking the supernatant; performing ultrafiltration separation on the digestion fluid by utilizing a membrane with the molecular cut off of 1kDa, collecting the filtrate until the light absorption value at 220nm is close to 0, concentrating, drying, dissolving, loading to a ToyopearlSP-650M column, and performing linear elution by using a NaCl-containing HAc-NaAc buffer solution; collecting and eluting active ingredients, concentrating and loading to a SephadexG10 column, eluting by using distilled water, collecting the eluted active ingredients, concentrating and drying to obtain odorless, tasteless and white powder, namely the target antioxidant collagen peptide.

The invention provides high-adhesion bi-component self-crosslinking digestive tract injury mucosa protection glue and application thereof. The high-adhesion bi-component self-crosslinking digestive tract injury mucosa protection glue comprises an aldehyde group-containing high polymer biological material and an amino group-containing high polymer biological material. The two components are mixed through a double-cavity injector and then injected to the surface of a digestive tract injury mucosa through an endoscope, aldehyde groups and amino groups in the two components are crosslinked to forma colloid membrane, the aldehyde group-containing high polymer biological material is combined with protein amino groups exposed on a wound surface and firmly adheres to the wound surface, the stimulation effect of intestinal digestive juice on the digestive tract injury mucosa is avoided, and healing of the wound surface is promoted. Animal tests show that the high-adhesion bi-component self-crosslinking digestive tract injury mucosa protection glue can obviously promote repair of the injury mucosa, and therefore the high-adhesion bi-component self-crosslinking digestive tract injury mucosaprotection glue has the effects of promoting healing and protecting esophagus, stomach and intestinal tract mucosa injuries and skin wound surfaces.

The invention relates to a preparation method and application of oral microspheres loaded with MSCs-derived exosomes. The preparation method comprises the following steps: (1) preparing an ALG / CST inner core solution; (2) preparing an ALG / CNC outer core solution; (3) separating exosomes derived from mesenchymal stem cells; and (4) preparing microspheres loaded with the MSCs exosomes. In the invention, hydrophobic ALG / CST and hydrophilic ALG / CNC are combined, the ALG / CST is used for wrapping the inter core of the exosomes, the defects of easy acidolysis of ALG, low drug loading of CNC andthe like are overcome, and the exosomes are effectively protected from being damaged by gastric acid and digestive juice. Meanwhile, with the pH-sensitive slow-release targeting property of small intestines, MSCs can be safely transported to the small intestines in an oral mode, damage repair, immunoregulation and anti-inflammatory effects are carried out by means of a small intestine absorption function, and the treatment of RA and other related autoimmune diseases is realized.

The present invention relates to the Chinese herbal medicine preparation including digestion liquid inside common nepenthes bag as main medicine component and pharmaceutical acceptable supplementary medicine material and its preparation process and application. By means of the digestion liquid inside common nepenthes bag, the medicine, after being introduced into blood vessel, can dissolve the embolism, hardened clots and inflammation factor inside blood vessel, decompose fat, cholesterol, saccharide and other composite macro molecular compound, promote blood circulation and dredge microcirculation. In addition, the medicine has common nepenthes and pubescent holly root as auxiliary medicine component, and this makes the medicine safe and high in treating effect. The present invention may be used together with blood vessel dilating Western medicine for wide use in treating various cardiac and cerebral vascular diseases.

The invention provides a method for culturing and subculturing intestinal cancer organoid derived from circulating tumor cells. The method comprises the following steps: (1) mixing a human colorectalcancer circulating tumor cell organoid culture medium with Matrigel according to a ratio of 1:1 to prepare a starting plate glue; (2) resuspending cells captured from the blood of a metastatic colorectal cancer patient by a cell filter with the pore diameter of 5.5 [mu]m by using the starting plate glue, and dropwise adding the cells into a 24-pore plate; (3) after the starting plate glue is solidified, adding the human colorectal cancer circulating tumor cell organoid culture medium around the starting plate glue, and culturing; (4) during subculturing, discarding the original culture medium,and adding a TrypLE digestive juice for digestion; and (5) stopping digestion by using a DMEM containing 10% of FBS, cleaning twice by using PBS, and re-suspending a starting plate by using the starting plate glue. The method for culturing and subculturing intestinal cancer organoid derived from the circulating tumor cells provides an experimental model for research on metastasis and drug resistance mechanisms caused by the circulating tumor cells, can perform drug sensitivity screening, and provides an effective basis for individualized treatment of advanced intestinal cancer.

Provided is a prevention and treatment of baby atopy using green tea and lactobacillus digestives. By addition of 0.02˜1.3 wt % of green tea, and for the purpose of preventing side effects from green tea and increasing curative efficacy, addition of 0.15˜10 wt % (0.009˜0.8 wt % on pure lactobacillus basis) of lactobacillus digestives made up of lactobacillus or butyric-acid bacteria or yeast fungi to baby dry milk or weaning food, we can prevent and treat baby atopy. 0.1˜1.3 wt % of green tea content is adequate for the cure of babies' atopy and 0.02˜0.1 wt % of green tea content is adequate for the prevention and aftercare of baby atopy. Green tea or green tea extract and lactobacillus digestives are added during manufacturing process of the baby dry milk or weaning food.

The present invention discloses an in-vitro constructed method for accurately predicting medication of patients with cancers. The method comprises the following steps of: 1) constructing an intestinalcancer organoid, namely culturing a purer intestinal cancer organoid by using cancer tissues obtained by operations or biopsies of patients with colon cancer, patients with rectal cancers or patientswith colorectal cancers; and 2) carrying out a large-flux drug screening test and carrying out a high-flux drug sensitivity detection by taking a micro organ as a unit. The in-vitro constructed method for accurately predicting the medication of the patients with the cancers has advantages that grinding the tissues enables digestion time to be shortened and a digestion degree is also consistent; mild digestive juice reduces damages of the step to cells; different pore diameters are used for filtering to screen the required cells more accurately; immune cells are removed to obtain purer cancercells; a self-made culture medium better conforms to components required by growth of intestinal cancer organs and a culture success rate is increased; and medicine screening is carried out by takingthe organoid as a unit, so that responses of the cancer tissues in vivo after clinical medication are better simulated.

The invention belongs to the technical field of biology, and specifically discloses an SD thoracic rat artery smooth muscle cell separation and culture method. The method comprises the following steps: (1) cutting SD rat thoracic artery in a sterile condition; (2) cutting the thoracic artery into long sections with a length of 3 to 5 centimeters, placing the sections in a PBS solution, cutting the thoracic artery in the vertical direction, removing the fat and connective tissues around the blood vessels, then soaking thoracic artery in collagenase (II); (3) taking out processed thoracic artery, and removing the external membrane; (4) incubating the thoracic artery in digestive juice, and then transferring thoracic artery to a DMEM culture medium; (5) transferring thoracic artery to a centrifuge, carrying out centrifugation, collecting the precipitate (namely thoracic artery smooth muscle cells), then placing the obtained thoracic artery smooth muscle cells into a fetal calf serum DMEM culture bottle, then transferring thoracic artery smooth muscle cells to an incubator, culturing the thoracic artery smooth muscle cells in the incubator, and then carrying out subculture. The provided method can greatly increase the number of obtained cells and improves the material utilization rate. Furthermore, the operation is simple and convenient.

The invention discloses a preparation method of digestion-free milk (containing goat milk) liquid and powder products. According to the preparation method, biological enzymolysis treatment is carriedout on milk or milk powder as a raw material to obtain products of digestion-free liquid milk and digestion-free milk powder. The digestion-free milk is small molecular protein with the molecular weight of below 500 Da (Dalton) and comprises the components of compound amino acid with the average molecular weight of 120 Da and small molecule dipeptide and tripeptide with the average molecular weight of 180-500 Da. This segment of protein does not need to be digested by a human body and can directly enter the body to be absorbed and utilized through small intestines of the human body. A proteinproduct which can be absorbed without digestion is provided for old people. After people get old, various functions of each body are degraded, digestive enzyme is reduced, the activity is decreased and eaten protein cannot be digested and absorbed, so that the digestion-free milk has great significance for supplementing the protein to the old people.

The invention discloses an RNA in-situ hybridization (ISH) kit for detecting overexpression of human epidermal growth factor receptor (EGFR) 2 (HER2) genes of breast cancer patients and belongs to the field of molecular pathological diagnosis. The kit comprises stationary solution, PBS-BufferI, PBS-BufferII, PBS-BufferIII, digestive juice, prehybridization solution, hybridization solution, Washing BufferI, Washing BufferII, buffer solution I, buffer solution II, blocking liquid, development solution I, development solution II, and a positive control tissue glass plate. Reference basis is provided for diagnosing positive breast cancer patients and important clinical value for early diagnosis of breast cancer and formulation of personalized medicines is provided by detecting the expression level of HER2.

The invention discloses a processing method of a ginger juice soft sweet, and belongs to the field of food processing. The processing method is characterized in that 10% of fresh ginger, 18% of stevia rebaudianum, 70-75% of saccharose, 3-5% of sodium alginate, 0.35-0.45% of malic acid, and 0.6-0.8% of sodium benzoate are used as raw materials, and the processing procedure is as follows: raw material processing-filtering-burdening-cooling forming-slitting-packing with glutinous rice paper-drying-packaging. The processing method has the beneficial effects that the ginger juice soft sweet is golden in color and luster, semi-transparent, strong in ginger taste, elastic and toughness, moderate in sweet and sour, and unique in ginger taste. The ginger juice soft sweet enriched in gingerol is capable of stimulating intestinal mucosa, promoting the secretion of saliva, gastric juice and digestive juice, increasing gastrointestinal motility, enhancing appetite, and playing the effects of appetizing and tonifying spleen, and promoting appetite, and the effects of relieving summer heat, resisting oxidation and inhibiting tumors. The processing method is simple to operate and convenient to use, and the ginger juice soft sweet is a nutritive and healthy delicious food.

The invention relates to a method for separating and culturing a human placental-derived mesenchymal stem cell. Aiming at problems and defects in an existing common PMSCs separation method, the technical scheme for solving the problems comprises the following steps: 1, taking out placenta by big tweezers, and cutting the placenta; 2, peeling the maternal surface and the infant surface of the placenta; 3, digesting placental villus; 4, diluting the digestive juice with 1000ml of PBS, filtering and centrifuging to collect cells for later use; 5, resuspending the cells with PBS, and centrifugally precipitating the cells; 6, subculturing the cells; and 7, freezing the cells. According to the method, PMSCs is separated by a one step digestive treatment with collagenase I, the operation steps can be reduced, cell damage can be lightened, the proportion of target cells can be effectively improved, and pollution of other cells can be reduced, so that the time for cell primary culture can be shortened, the cell purity can be improved, the cost can be greatly reduced, the original state of PMSCs can be well maintained, and a firm foundation is laid for massive production and wide clinical application of PMSCs in the future.

The invention relates to an animal feed with brand new compositions. The animal feed consists of a premixed feed, a premixed feed companion and an energy nutritional feed, wherein the premixed feed is formed by amino acid, vitamins, inorganic salt, antibiotics, digestant and other substances required by animal growth; the nutritional feed can be protein nutritional feed; and the energy nutritional feed can be corn, wheat or barley.

The invention relates to a preparation method and a product of pH-sensitive digestive tract injured mucosa protective glue suitable to be sprayed on the surface of digestive tract injured mucosa by anendoscope, and belongs to the field of biological medicine preparations. The digestive tract injured mucosa protective glue comprises colloidal liquid and stationary liquid, wherein the colloidal liquid comprises a pH-sensitive biological material, a pH regulator, a biological adhesive, an ion-sensitive biological material and a dissolving solution, and the stationary liquid is a water-soluble calcium ion solution. Under the condition of low pH, the product is liquid and forms a colloidal film under the condition of physiological pH, so that the stimulation effect of intestinal digestive juice on digestive tract injured mucosa is avoided, and the healing of a wound surface is promoted. An animal test shows that the protective glue can obviously promote the repair of the injured mucosa, sothat the protective glue has the healing promoting and protecting effects for esophagus mucosa, intestinal mucosa and skin injury.

The invention discloses a papaw floss bread, which comprises the following raw materials of 110 to 130 parts of flour, 30 to 40 parts of papaw, 11 to 13 parts of edible oil, 3 to 4 parts of yeast, 3 to 5 parts of honey, 5 to 7 parts of scallion, 3 to 4 parts of ginger, 0.8 to 1 part of sunflower disc, 0.8 to 1 part of radix codonopsitis, and proper parts of edible salt. The produced bread has the advantages that the color is bright, and the taste is good; the added papaw contains a great amount of carotene, protein, calcium salt, protease, lemon enzyme and the like, so the functions of preventing and controlling hypertension, nephritis and constipation, assisting digestion and treating stomach illness are realized, the functions of promoting metabolism and resisting senescence of human bodies are realized, and the effects of beautifying faces and caring skins are realized; and meanwhile, the function of promoting digestion by the radix codonopsitis and the sunflower disc for prompting digestive juice to secrete is realized.

The present invention discloses a tender ginger preserve processing technology which belongs to the food processing field. The processing technology is characterized by being composed of the following processing technology flow: selecting materials, peeling ginger skins, puncturing, bleaching with lime water, pinching, sugar simmering, removing from the pot, taking the treated gingers out, wrapping sugar and packaging. The beneficial effects of the tender ginger preserve are that the preserve has a pale color, a delicate and crisp taste, is fragrant, sweet and delicious, and has a mellow ginger and a unique tender ginger flavor. The tender ginger preserve is rich in the specific gingerol, can stimulate gastrointestinal mucosa, promote secretions of saliva, gastric juice and digestive juice, increase gastrointestinal motility and promote appetite, has efficacies of stimulating appetite, tonifying spleen and promoting appetite, as well as relieving summer heat, refreshing oneself, prevent oxidation and prevent tumors. The processing technology is simple to operate and easy to use, and the tender ginger preserve is a nutritious, healthy and green food.