Culture method of breast cancer organoid and co-culture method of tumor-associated fibroblasts

Abstract

The invention relates to a culture method of breast cancer organoids and a co-culture method of tumor-related fibroblasts. According to the invention, a more efficient and convenient improved system for key technical links such as PDO culture, passage, maintenance and the like of tumor tissue organoids of breast cancer patients is established, operation steps and methods for establishing and passage of a PDO cell line are simplified in multiple aspects, components of a culture medium, digestive juice, a washing solution and the like are optimized, and finally, a breast cancer PDO culture system is successfully established. Compared with the prior art, the culture method has the advantages that the success rate and the passage frequency are basically consistent, but the operation is simpler, the required time is shorter, a good foundation is laid for a PDO model to have better clinical and scientific research applications in the future, and the corresponding cost is saved. The inventionalso establishes a stable co-culture system of breast cancer PDO and tumor-associated fibroblasts (CAFs), simultaneous culture, amplification and passage of PDO and CAFs can be realized, and the PDOand the CAFs do not produce obvious influence on each other.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for culturing breast cancer organoids and a method for co-cultivating tumor-related fibroblasts. Background technique [0002] Breast cancer (Breast Cancer, BC) is the leading cause of death from malignant tumors in women. Globally, China accounts for 12.2% of newly diagnosed cases and 9.6% of BC deaths. According to the latest domestic epidemiological statistics, the incidence of breast cancer ranks first among female malignant tumors, and the mortality rate ranks fifth among female malignant tumors. Breast cancer is a highly heterogeneous cell population that can be divided into several subtypes. Today, breast cancer research relies heavily on established cell lines, which are difficult to maintain in terms of maintaining the cellular biology and behavior of the primary tumor. In clinical treatment, the heterogeneity of breast cancer plays a very important role in guiding ...

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Problems solved by technology

[0004] However, the medium for the above culture methods is expensive and the operation is cumbersome.

At present, there is no more concise, cheap and efficient breast cancer organoid culture model as described in this application, and there is no co-culture system model of breast cancer organoids and tumor-associated fibroblasts (Cancer associated fibroblasts, CAFs)

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