42 results about "Deinococcus radiodurans" patented technology

This invention provides novel truncation mutants of a phytochrome from the bacterium Deinococcus radiodurans. When expressed either in bacteria or mammalian cells, these mutant phytochromes spontaneously incorporate biliverdin, a ubiquitous intermediate in heme catabolism, and become fluorescent in the infrared (IR) region. These phytochromes are the first genetically encoded labels that can be excited by far-red light and fluorescent in the true IR (>700 nm). If these mutants instead incorporate protoporphyrin IX, an intermediate in heme biosynthesis, illumination now generates significant amounts of singlet oxygen. Singlet oxygen is useful because it can be used to kill individual proteins or cells, detect long-range protein-protein interactions, or generate electron-microscopic contrast. The invention also relates to methods of making and using such proteins and protein variants.

The invention discloses a gap DNA-preferential high-fidelity polymerase and its application. The invention discloses that the high-fidelity polymerization characteristic of the Klenow fragment (KlenDr) derived from the DNA polymerase I of Deinococcus radiodurans is not dependent on the 3'-5' corrective excision activity, and has a preference for binding to nick DNA, different Compatible with existing commercial high-fidelity polymerases. Due to the specific affinity of KlenDr to the gap DNA substrate, the 3' end of the upstream primer will not be excised, and the downstream nucleotide chain will rarely be replaced. It will have a very broad application prospect in genetic engineering operations such as gap filling and the construction of sequencing libraries.

The invention relates to a method for synthesizing 5-hydroxymethylfuroic acid by using Deinococcus radiodurans R1, inoculating Deinococcus radiodurans R1 on a TGY solid medium by streaking, picking a single colony and inoculating it on a TGY liquid medium after cultivation, After culturing to the logarithmic growth phase, insert it into fresh TGY liquid medium according to the inoculation amount of 1%, cultivate for 48 hours, collect the bacterial cells, add them to the buffer solution containing 5-hydroxymethylfurfural, and heat them at a temperature of 20-60 Reaction at ℃ for 3-48 hours to obtain 5-hydroxymethylfuroic acid. Deinococcus radiodurans R1 used in the present invention is used as a biocatalyst, which has high tolerance to 5-hydroxymethylfurfural, can catalyze the selective oxidation of high-concentration substrates to synthesize the target product, and the yield is more than 86%. The method of the present invention It has the characteristics of higher substrate concentration, better reaction efficiency and good selectivity.

The invention discovers that trkB genes (DR1667) in Deinococcus radiodurans R1 are capable of enhancing resistance capability of prokaryotes and plants. Recombinant vectors comprising the trkB genes (DR1667) are constructed and are respectively transferred into prokaryotic and eukaryotic host cells. Experiments prove that salt tolerance of the prokaryotic host cells and rape can be enhanced after the trkB genes (DR1667) are expressed in the prokaryotic host cells and rape.

The invention finds that a ytxH gene (DR0105) in Deinococcus radiodurans R1 has the function of improving the adversity resistance of prokaryotes and plants. The invention constructs recombinant vectors containing the gene and the recombinant vectors are respectively transferred into prokaryotic and eukaryotic host cells. Experiments prove that the ytxH gene (DR0105) can improve the salt tolerance of prokaryotes and the transgenic plants obtained after transplanting the gene into the plants also have salt tolerance.

The invention discovers that dap gene (DRB0118) in Deinococcus radiodurans R1 improves the resistance of prokaryotes and plants. The invention constructs a recombinant vector comprising the gene; and the recombinant vector is transferred into prokaryotic and eukaryotic host cells. Experiments prove that: after the dap gene (DRB0118) is expressed in prokaryotic host cells and tobacco, the salt resistance of the prokaryotic host cells and the tobacco can be improved.