86 results about "Chlorin e6" patented technology

Siderophore-photosensitizer conjugates, their synthesis and use in photodynamic antimicrobial therapy (PACT) is disclosed. The advantage of this method is improvement of photodynamic antimicrobial therapy against, for example, pathogenic micro-organisms such as bacteria and fungi. Naturally occurring and synthetically available siderophore structures are conjugated chemically with photoactive compounds such as Chlorin e6 to improve their penetration into bacterial cells and to increase antibacterial efficacy of photosensitizers via microbial proteins that recognize and transport iron-loaded siderophores. In this way, photosensitizers can be transported inside bacteria that otherwise could not cross the cell wall and membranes. Photodynamic activation of photosensitizers inside the cells of pathogenic microbes enables a more effective inhibition of cellular functions than application at the outer side of the cells. The siderophore-transporting systems of microbes are known to be specific for bacteria and fungi. Consequently, siderophore conjugates with photosensitizers are not taken up by mammalian cells and photodynamic effects can thus be exerted specifically on pathogenic microbes. Applications of these conjugates include highly efficient treatment of pathogenic gram-negative and -positive bacteria such as Pseudomonas aeruginosa, Escherichia coli, Streptococcus pyogenes, Staphylococcus aureus, treatment of microbial infections that often occur in chronic wounds as well as therapy of other antibiotic resistant microbial infections.

The invention provides thermo-sensitive liposome. The thermo-sensitive liposome comprises a chemotherapeutic drug, a photosensitizer, phosphatidylcholine and polyethylene glycol-derived phospholipid, wherein the photosensitizer is indocyanine green or chlorins e6; the grain size of the thermo-sensitive liposome is 40-60 nm; by virtue of living-body fluorescence imaging, a distribution process of the thermo-sensitive liposome can be observed in real time; under a near-infrared light irradiation condition, the chemotherapeutic drug can be quickly and effectively released, so that the chemotherapeutic drug can be enriched at a target part very well; the obtained thermo-sensitive liposome particle has smaller particle diameter less than 100 nm, and good biocompatibility. The preparation method of the thermo-sensitive liposome is simple and easy to operate.

The invention belongs to the technical field of biological medicine, and relates to a lipidosome preparation, and a preparation method and application thereof, in particular to lipidosome-dimethyldiguanide-chlorin e6, and preparation and application thereof, particularly application to tumor photodynamic treatment and radiotherapy. The lipidosome preparation (lipidosome-dimethyldiguanide-chlorin e6) provided by the invention consists of a lipidosome, dimethyldiguanide and chlorin e6; the particle size is uniform; the dispersion in water is good; the particle size in water is mainly about 110nm; photosensitive molecules and the dimethyldiguanide can be simultaneously loaded; the lipidosome preparation is an ideal medicine carrier and has good stability. Experiments show that the lipidosome-dimethyldiguanide-chlorin e6 can be highly enriched in the tumor position; a continuous effect is achieved for lowering the tumor cell oxygen consumption quantity; the anoxia condition of the tumor position can be obviously improved; a good photodynamic treatment and radiotherapy effect is achieved.

The invention relates to a chlorin and artesunate compound with photosensitive and sound-sensitive activity as well as a preparation method and an application of the compound and belongs to the technical field of chemical medicines. The chlorin and artesunate compound with photosensitive and sound-sensitive activity has different degrees of inhibition effects on human hepatoma cells Hep G2 in in-vitro anti-tumor activity evaluation. The photoactivity and ultrasonic activity are both higher than that of chlorin e6 and artesunate which are used as positive controls. The compound can be used for preparing a photosensitizer and a sound-sensitive agent in photodynamic therapy and sonodynamic therapy methods for tumor therapy.

The invention provides a preparation of chlorin e6 chitosan-stearic acid graft micelle. The preparation comprises a chitosan-stearic acid graft and chlorin e6, wherein, the chlorin e6 accounts for 4.76-16.76% of the total weight; the chitosan-stearic acid graft is formed by chemically grafting low molecular weight chitosan with average molecular weight being 18.0 KDa and stearic acid; and the amino substituted ration of the chitosan-stearic acid graft accounts for 4.96%. The invention utilizes the chitosan-stearic acid graft micelle to effectively pack the photosencitizer of chlorin e6, so as to prepare the intracellular administration preparation of chlorin e6 chitosan-stearic acid graft micelle. Compared with the chlorin e6 solution, the preparation of the invention can significantly improve the drug concentration of chlorin e6 in tumour cells and provide probability for the follow-up realization of efficient and harmless photodynamic therapy of tumors.

The invention relates to novel chlorin e6 derivatives, pharmaceutically acceptable salts thereof, as well as preparation methods and applications of the novel chlorin e6 derivatives and the pharmaceutically acceptable salts and belongs to the technical field of medicines. The chlorin e6 ether amino acid derivatives comprise compounds shown as the general structural formula I and optical isomers ofthe general structural formula I. The preparation method of the novel chlorin e6 derivatives comprises steps as follows: 3-vinyl in chlorin e6 is etherified, and 15-ethylcarboxyl and amino acid are subjected to peptides formation. The chlorin e6 ether amino acid derivatives and the pharmaceutically acceptable salts thereof can be taken as photodynamic antitumor drugs for application. Compared with an existing clinically applied similar photosensitizer talaporfin, the chlorin e6 ether amino acid derivatives have the advantages of being high in photodynamic antitumor activity, high in ratio ofdark toxicity to phototoxicity and the like and can be applied to preparation of new photodynamic antitumor drugs including photodynamic cancer treating drugs, photodynamic treatment drugs for benignvascular diseases such as senile macular degeneration and nevus flammeus as well as photodynamic treatment drugs for condyloma acuminate.

The invention relates to the technical field of biological medicines, and in particular relates to a protein-polypyrrole compound as well as a preparation method and application of a protein-polypyrrole compound derivative. The pyrrole in the protein-polypyrrole compound provided by the invention is wrapped on the surface of protein in an in situ polymerization manner. The protein-polypyrrole compound can generate very high heat under illumination of laser after being swallowed by cells, and can kill tumor cells, so that the protein-polypyrrole compound is a good photo-thermal reagent and can be used as a carrier for carrying a photosensitizer. The pyrrole in the protein-polypyrrole compound derivative provided by the invention is wrapped on the surface of the protein carrying chlorin e6 in an in situ polymerization manner, and is an ideal photo-thermal therapeutic agent, a photodynamic therapeutic agent or a photo-thermal and photodynamic combined therapeutic agent. Moreover, because chlorin e6 can be used for fluorescence imaging of tumors, the invention also discloses an application of the protein-polypyrrole compound derivative serving as an imaging agent of the tumors.

The present invention relates to a novel chlorin e6-folic acid conjugate, a preparation method thereof, and a pharmaceutical composition for the treatment of cancer comprising the same, and more particularly, to a novel compound prepared by linking chlorin e6 to folic acid, which effectively produces singlet oxygen in various media and has much better tumor selectivity than the known porphyrin-based photosensitizers, thereby being used effectively in photodynamic therapy for malignant tumors, a preparation method thereof, and a pharmaceutical composition for photodynamic treatment of solid tumors comprising the compound.

The invention discloses an antibacterial material with pH responsivity and light sensitivity and a preparation method thereof. Hydrophilic and hydrophobic photodynamic molecules are loaded in a nano particle polymer Poly(HDDA-co-DBPA)-PEG. High molecular polymer nano particles with pH responsivity are prepared and are subjected to hydrophilic PEG modification, and photodynamic molecule medicines Chlorin e6 are loaded into the nano particles by hydrophilic and hydrophobic properties. Multiple biological antibacterial technologies are integrated, the bactericidal biological effect is increased, the adhesion of medicines in special environments is effectively increased, the bacterial organism growth is inhibited, an effective antibacterial effect can be achieved, the biocompatibility is good, the cost is low, and the material stability is good. A biological macromolecular antibacterial material obtained by the preparation method disclosed by the invention has the characteristics of effective antibacterial effect, good biocompatibility, good stability and low cost.

The present invention relates to a method for preparing chlorophyll-a and chlorin e6. This invention extracts chlorophyll-a by use of undisrupted chlorella cells themselves, thereby preparing chlorin e6 from the chlorophyll-a extract. The high contents of chlorophyll-a may be obtained by the pretreatment procedure of chlorella cells themselves selected in this invention. The present method is performed according to relatively simple procedures, and is suitable in the mass production of chlorin e6.

An optochemical sensor element suitable for sensing an analyte comprises a polymeric material in which at least a portion of the polymer material is solvent crazed to provide a multiplicity of pores of controlled nanometer size, and an indicator dye impregnated into the pores, in which optochemical sensor element the indicator dye is a long-decay photoluminescent dye selected from the group consisting of: phosphorescent platinum(II)—and palladium (II) complexes of porphyrin dyes such as octaethylporphine, coproporphyrin, octaethylporphine -ketone, benzoporphine, tetra (pentafluorophenyl) porphine, chlorin e6; fluorescent complexes of ruthenium (II), osmium(II), iridium(III) and europium (III); or derivatives or close analogs of these dyes. The sensor element in the presence of the analyte alters a photoluminescence parameter thus allowing sensing and / or quantification of the analyte.

The invention belongs to the field of medical chemistry and particularly relates to a preparation method for Talaporfin and an intermediate thereof. The Talaporfin is obtained by taking a compound chlorins e6 mono aspartic acid-4-ester as a key intermediate and hydrolyzing the chlorins e6 mono aspartic acid-4-ester. The preparation method for the Talaporfin and the intermediate thereof has the advantages that the product purity is improved; the operation is simplified; requirements on equipment are lowered; and industrial production is easy.

The invention discloses a chlorins glucoside compound, and a preparation method and application thereof, and belongs to the technical field of chemical medicines. According to the chlorins glucoside compound disclosed by the invention, through the selective combination of the glucose transport protein which is highly expressed on the surface of the tumor cells, the targeting effect of a photosensitizer and a sound-sensitive agent on tumor cells is improved. The in-vitro anti-tumor activity evaluation shows that compared with chlorins e6 which is used as a control, the chlorins glucoside compound provided by the invention has relatively high light activity and sound activity on humanhepatoma carcinoma cells Hep G2. The chlorins glucoside compound disclosed by the invention can be used for preparation of a photosensitizer and a sound-sensitive agent in photodynamic therapy and sonodynamics therapy methods for tumor therapy. According to the invention, the preparation process is simple, the reaction conditions are easy to control, and the production is facilitated.

The invention belongs to the field of pharmaceutical preparations and relates to a drug delivery system for targeting co-delivery of a photosensitizer and a chemotherapeutic drug. According to the drug delivery system, a nanometer preparation is prepared from vitamin E polyethylene glycol succinate-L-polylactic acid as a raw material, a chemotherapeutic drug adriamycin is wrapped in a hydrophobic inner core of the nanometer preparation, simultaneously, a photosensitizer chlorin e6 is connected to vitamin E polyethylene glycol succinate (TPGS) through a covalent bond, then the compound is inserted into a shell structure of the nanometer preparation so that efficient and stable coating of the chemotherapeutic drug and the photosensitizer is realized, and a polypeptide tLyp-1 having a targeting function modifies the surface of the nanometer preparation through a covalent bond so that nanometer preparation vascular permeability and tumor penetrability are promoted. Results of in-vitro and in-vivo experiments show that the drug delivery system is used for treatment on tumors resisting multiple drugs, has the characteristics of good targeting ability, high efficiency and low toxicity, and has a clinical application prospect.

The invention relates to tyroserleutide-chlorin e6 monoester prepared by esterification of the tyroserleutide with the chlorin e6. With the tyroserleutide as a water soluble polar end, the tyroserleutide-chlorin e6 monoester can dissolve in water. Consequently, the tyroserleutide-chlorin e6 monoester has the advantages of solving the problem of poor water solubility of Ce6, having double antitumoreffects of the chlorin e6 and the tyroserleutide, being to photodynamic and sonodynamic therapeutic drugs, and being applicable to prepare drugs for treating skin diseases and malignant tumors.

The invention relates to a preparation method and application of phycocyanin-chlorin e6 covalent nanoparticles, and belongs to the technical field of medical functional materials. The preparation method comprises the following steps: firstly performing activation on chlorin e6 at normal temperature, adding the activated chlorin e6 dropwise into a phycocyanin solution dissolved in PBS, performing areaction for a period of time, taking out the solution, performing centrifugation, performing dialysis, and performing lyophilization to obtain the powder. The method uses phycocyanin to target tumorcells and uses laser as a light source, irradiation is performed after a photosensitizer is enriched in tumors, so that the output of active oxygen in photodynamic therapy can be greatly improved, tumor growth can be inhibited, and the therapeutic effect is significantly enhanced.

The invention discloses a branched polyetherimide material containing a ketone thioacetal bond, as well as a preparation method and application thereof. The preparation method comprises the followingsteps: (1) stirring acetone and acid containing thiol to react at room temperature; (2) adding polyetherimide and an activator after the reaction is finished, dissolving in an organic reagent, stirring to react, bonding chlorin e6 and carboxyl polyethylene glycol through an amidation reaction to obtain the reactive oxygen sensitive branched polyetherimide material containing the ketone thioacetalbond. The branched polyetherimide material has excellent biocompatibility and degradability, is entrapped with siRNA, and forms nano-particles by self-assembly, can generate a great number of reactiveoxygen under excitation of a light source with specific wavelength to destroy the structure of endosome, the reactive oxygen sensitive ketone thioacetal bond is broken, particles are disintegrated, and endosome escape and release of intracellular siRNA can be accelerated. The branched polyetherimide material containing a ketone thioacetal bond has a huge clinical application potential in the field of tumor treatment.

The invention relates to a chlorins e6 metal salt compound.The chemical structure is shown in the formula (I) in the description, wherein R is common metal ions such as K+ or Na+.A preparing method comprises the following steps that firstly, silkworm excrement crude product chlorophyll serves as a raw material and is dissolved in diethyl ether, concentrated hydrochloric acid is added, and magnesium-removed chlorophyllin a is generated; secondly, magnesium-removed chlorophyllin a is reacted in a strong-base methanol solution, a little of water is added later to generate chlorins e6 mono-methyl ester; thirdly, obtained chlorins e6 mono-methyl ester is reacted in a strong-base alcoholic solution reaction reagent, and a compound (I) is obtained through separation and purification.The chlorins e6 metal salt compound is a raw material in the study field of medicine for resisting gastric ulcers, resisting anemia and protecting the liver and promoting leucopoiesis.

The invention provides a nanocarrier medicine, as well as a preparation method and an application of the nanocarrier medicine. The nanocarrier medicine takes polylactic acid-glycolic acid as a carrier, and is loaded with NVP-BEZ235 and chlorin E6, and the surface of a carrier is subjected to PEGylation by using lecithin and distearoyl phosphoethanolamine-polyethylene glycol. The polylactic acid-glycolic acid is taken as the carrier, the chlorin E6 and the NVP-BEZ235 are combined to serve as a therapeutic agent, and the photodynamics therapy and the biotherapy are combined and produce a synergistic effect to improve the killing effect on a tumor cell. The lecithin and the DSPE-PEG are used to conduct PEGylation on the surface of a nanoparticle, the time for blood circulation is prolonged, the clearing of reticuloendothelium is reduced, the therapeutic effect is further guaranteed, so that the therapeutic effect of tumors, particularly the triple negative breast cancer, is obvious, and the nanocarrier medicine has a broad application prospect.

A method of treatment of retinitis pigmentosa using a medically effective dose of insulin, IGF-1, and chlorin e6 topically applied to the conjunctival sac of the afflicted eye. The combination of these is very effective in treating retinitis pigmentosa and may be repeated as directed by a medical practitioner. The method includes preparing the dosage and filling an eye dropper with the compound, then having the patient lie in a supine position while administering the dosage. The patient remains in this position for 5 minutes to ensure absorption of the compound. In one embodiment, single use eye droppers are provided to simplify treatment. The particular dosage is adjusted to take individual metabolisms into account. A thorough examination of the patient's eyes should be done prior to treatment.

The invention relates to a double-receptor targeting drug delivery system realizing the synergistic effect of phototherapy and chemotherapy, as well as preparation and applications of the double-receptor targeting drug delivery system. The system is a compound containing hyaluronic acid, polylactic acid, polypeptide, and chlorambucil / chlorin e6, wherein the molecular weight of polylactic acid is 8000Da, specifically, the compound comprises the following components in parts by weight: 100-2000 parts of hyaluronic acid, 1500-10000 parts of polylactic acid, 21-450 parts of N,N'-dicyclohexylcarbodiimide, 12-240 parts of N-hydroxy succinimide, 1-30 parts of polypeptide, 20-400 parts of 1-(3-(Dimethylaminopropyl)-3-ethylcarbodiimide, 100-1000 parts of chlorambucil, and 50-600 parts of chlorin e6. The double-receptor targeting drug delivery system has the beneficial effects that (1) the synergistic effect of phototherapy and chemotherapy is realized; (2) the multiple targeting properties are realized; (3) the good blood stability is realized; (4) the system has small toxicity and high safety.

The invention provides a preparation method of a chitosan-chlorin e6 antibacterial agent. In the chitosan-chlorin e6 antibacterial agent, chlorin e6 accounts for 13.48%-22.04% of the total weight of the antibacterial agent; chitosan-chlorin e6 is obtained by combining chitosan and the chlorin e6 through an amido bond; and the amino substitution degree of the chlorin e6 to the chitosan in the chitosan-chlorin e6 is 6.44%-11.68%. According to the invention, the chitosan is used as a carrier of a photosensitizer, namely the chlorin e6, and the chitosan-chlorin e6 antibacterial agent is prepared. Compared with the chlorin e6, the antibacterial agent disclosed by the invention has the advantages that the antibacterial activity of the chlorin e6 on staphylococcus aureus and escherichia coli can be effectively improved, and the antibacterial agent has application value in various fields including agriculture, medicine and the like.

The invention provides a chlorin e6 ferrocene conjugate with photo- and acoustic-sensitive activity as well as a preparation method and application thereof, and belongs to the technical field of chemical medicines. The chlorin e6 ferrocene conjugate disclosed by the invention has different degrees of inhibition effects on Hela cells in in-vitro anti-tumor activity evaluation. The introduction of the ferrocene group significantly improves the proliferation inhibition activity of the compound on tumor cells, and the proliferation inhibition activity of the chlorin e6 ferrocene conjugate on tumorcells is much higher than that of chlorin e6 used as a control group. The method can be used for preparing photosensitizers and sonosensitizers in photodynamic therapy and sonodynamic therapy methodsfor tumor therapy.

The invention discloses a chlorin e6-lentinan ester and a preparation method thereof. The chlorin e6-lentinan ester is prepared by the esterification reaction between the lentinan and the chlorin e6,and the lentinan is the polar end of soluble in the water, so that the chlorin e6-lentinan ester is dissolved in water to solve the problem of poor water solubility of Ce6, and has the double anti-tumor effect of the chlorin e6 and the lentinan, and belongs to the field of light and sound power treatment medicaments, and can be used for preparing medicaments for treating skin diseases, malignant tumors or cancerous pleural and abdominal water.

The present invention relates to a pharmaceutical composition or a cosmetic composition, comprising, as an active ingredient, chlorin e6 having excellent antibacterial activity against Propionibacterium acnes.

The invention discloses a preparation method of a chlorin e6 and ferroferric oxide composite nanoparticle and belongs to the technical field of photosensitizer nanocrystallization. The preparation method of the chlorin e6 and ferroferric oxide composite nanoparticle comprises the following steps of preparing ferroferric oxide nanoparticles coated with oleic acid; preparing the chlorin e6 and ferroferric oxide composite nanoparticle. Under the condition of not changing the structure and the functions, the preparation method of the chlorin e6 and ferroferric oxide composite nanoparticle solves the problem of poor water solubility of the photosensitizer of chlorin e6 in the prior art, enhances the water solubility of the photosensitizer of chlorin e6 and further improves blood circulation stability and dispersibility. Meanwhile, the preparation method of the chlorin e6 and ferroferric oxide composite nanoparticle can enhance the superparamagnetism of the photosensitizer of chlorin e6.

The invention belongs to the field of photodynamic therapy, and particularly relates to application of chlorin e6 trimeglumine salt in photodynamic therapy of cervical cancer and precancerous lesions.