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Protein-polypyrrole compound as well as preparation method and application of protein-polypyrrole compound derivative

A technology of polypyrrole and compound, which is applied in the field of biomedicine, can solve the problem that there is no practical and effective method for tumors, achieve good water solubility and biocompatibility, improve absorption effect, good water solubility and biocompatibility Effect

Active Publication Date: 2015-05-06
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there is no practical and effective method for the treatment of tumors. Therefore, the combined use of multiple technical solutions or the combined use of multiple effective substances to achieve better treatment of tumors is the focus of current research.

Method used

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  • Protein-polypyrrole compound as well as preparation method and application of protein-polypyrrole compound derivative
  • Protein-polypyrrole compound as well as preparation method and application of protein-polypyrrole compound derivative
  • Protein-polypyrrole compound as well as preparation method and application of protein-polypyrrole compound derivative

Examples

Experimental program
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Effect test

Embodiment 1

[0074] The preparation of embodiment 1 protein-polypyrrole complex

[0075] Weigh 20 mg of bovine serum albumin and dissolve it in 5 mL of water, and ultrasonically dissolve to obtain a bovine serum albumin solution.

[0076] Dissolve 20 μL of pyrrole (mass ratio of protein to protein: 1:1) in 1 mL of deionized water, and sonicate to obtain a pyrrole solution.

[0077] Ferric chloride hexahydrate was dissolved in deionized water to obtain FeCl with a concentration of 1ml and a concentration of 20mg / ml 3 solution.

[0078] Add the pyrrole solution dropwise to the bovine serum albumin solution, stir at room temperature and normal pressure for 1 h, then add ferric chloride solution dropwise, and stir at room temperature and normal pressure for 24 h. Prepare protein-polypyrrole complex.

Embodiment 2

[0079] Preparation of Example 2 protein-polypyrrole complex

[0080] Weigh 20 mg of transferrin and dissolve it in 5 mL of water, and ultrasonically dissolve to obtain a transferrin solution.

[0081] Dissolve 20 μL of pyrrole (mass ratio of protein to protein: 1:1) in 1 mL of deionized water, and sonicate to obtain a pyrrole solution.

[0082] Ferric chloride hexahydrate was dissolved in deionized water to prepare 1ml of FeCl with a concentration of 20mg / ml 3 solution.

[0083] Add the pyrrole solution dropwise to the transferrin solution, stir at room temperature and normal pressure for 1 h, then add ferric chloride solution dropwise, and stir at room temperature and normal pressure for 24 h. Prepare protein-polypyrrole complex.

Embodiment 3

[0084] The property detection of embodiment 3 protein-polypyrrole complex

[0085] Qualitative detection was performed on the protein-polypyrrole complexes prepared in Examples 1-2, and dynamic light scattering, ultraviolet absorption, and transmission electron microscope detection were performed respectively.

[0086] Among them, the results of dynamic light scattering detection are as follows figure 1 As shown, the results show that the particle size of the protein-polypyrrole complex provided by the present invention is mainly concentrated in 16nm-22nm in water.

[0087] The results of transmission electron microscopy were as follows: figure 2 As shown, the results show that the protein-polypyrrole complex provided by the present invention is distributed in a monodisperse state in water, indicating that the protein-polypyrrole complex prepared by the present invention has a uniform particle size and is well dispersed in water.

[0088] The results of the UV absorption test...

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Abstract

The invention relates to the technical field of biological medicines, and in particular relates to a protein-polypyrrole compound as well as a preparation method and application of a protein-polypyrrole compound derivative. The pyrrole in the protein-polypyrrole compound provided by the invention is wrapped on the surface of protein in an in situ polymerization manner. The protein-polypyrrole compound can generate very high heat under illumination of laser after being swallowed by cells, and can kill tumor cells, so that the protein-polypyrrole compound is a good photo-thermal reagent and can be used as a carrier for carrying a photosensitizer. The pyrrole in the protein-polypyrrole compound derivative provided by the invention is wrapped on the surface of the protein carrying chlorin e6 in an in situ polymerization manner, and is an ideal photo-thermal therapeutic agent, a photodynamic therapeutic agent or a photo-thermal and photodynamic combined therapeutic agent. Moreover, because chlorin e6 can be used for fluorescence imaging of tumors, the invention also discloses an application of the protein-polypyrrole compound derivative serving as an imaging agent of the tumors.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method and application of protein-polypyrrole complexes and protein-polypyrrole complex derivatives. Background technique [0002] Cancer is one of the major malignant diseases that threaten human health. In cancer treatment, traditional surgical therapy, radiotherapy and chemotherapy will damage normal tissues in the body and bring some other side effects. In recent years, new therapeutic approaches, such as photothermal therapy (PTT) utilizing near-infrared photothermal conversion in near-infrared lasers, have begun to be investigated for cancer treatment. The basic principle of photothermal therapy is to use the high heat generated by photothermal conversion to destroy and eliminate cancer cells under the condition of laser irradiation. Among them, the strong near-infrared optical absorption and high photothermal conversion efficiency on the cancer cell site ...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/48A61K49/00A61P35/00
Inventor 刘庄宋雪娇
Owner SUZHOU UNIV
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