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37 results about "Amino acid sequence homology" patented technology
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Sequence Homology, Amino Acid. Subject Areas on Research 14-3-3 proteins are part of an abscisic acid-VIVIPAROUS1 (VP1) response complex in the Em promoter and interact with VP1 and EmBP1. 27-hydroxycholesterol is an endogenous selective estrogen receptor modulator.
Methods for calculating codon pair-based translational kinetics values, and methods for generating polypeptide-encoding nucleotide sequences from such values
InactiveUS20070275399A1Improve shortcomingsEasy to analyzeMicrobiological testing/measurementData visualisationNucleotideNucleotide sequencing
Provided are methods for calculating codon pair translational kinetics values, creating a synthetic gene for expression in a host organism, and providing codon pair translational kinetic values. The methods typically are directed to refinement of statistical observed versus expected codon pair frequencies using one of several factors such as amino acid sequence homology, secondary or tertiary structural considerations, and empirical measurements. In some synthetic genes codon pairs are predicted not to cause a translational pause in the host organism, thereby providing a polynucleotide sequence encoding the desired polypeptide with desired translational kinetics properties. The methods can be performed using multiple parameter nucleotide sequence optimization methods, such as branch-and-bound methods for nucleotide sequence refinement.
Owner:LATHROP RICHARD H +4
Attenuated strain of Leishmania
Differentially expressed Leishmania genes and proteins are described. One differentially expressed gene (A2) is expressed at significantly elevated levels (more than about 10 fold higher) in the amastigote stage of the life cycle when the Leishmania organism is present in macrophages than in the free promastigote stage. The A2 gene encodes a 22 kD protein (A2 protein) that is recognized by kala-azar convalescent serum and has amino acid sequence homology with an S-antigen of Plasmodium falcilparum Vietnamese isolate VI. Differentially expressed Leishmania genes and proteins have utility as vaccines, diagnostic reagents, as tools for the generation of immunological reagents and the generation of attenuated variants of Leishmania.
Owner:MCGILL UNIV
Peptides for stimulating an immune response against melanoma
Provided in the present invention are recombinant peptides and a method for using the peptides in stimulating an immune response against human high molecular weight-melanoma associated antigen (HMW-MAA). The peptides were designed from the identification of regions of structural and amino acid sequence homology between HMW-MAA and the mouse anti-idiotypic monoclonal antibody MK2-23. The method comprises the step of administering to an individual a peptide of the invention in an amount effective to elicit an immune response against HMW-MAA.
Owner:HAUPTMAN WOODWARD MEDICAL RES INST +1
Method for cloning rice auxin induced protein gene
InactiveCN101492671AHigh homologySimple implementation stepsFermentationPlant genotype modificationMutantDNA Intercalation
The invention relates to a method for using rice auxin to induce protein genes to be cloned, which is characterized in that the implementation steps comprise: (1) the preparation of a rice transformation receptor; (2) the genetic transformation of the rice; (3) the screening of kanamycin-resistant callus tissue and the regeneration of plants; (4) the screening of the mutant of a T-DNA inserted progenies; (5) Tail-PCR; (6) the comparison and analysis of sequences on the Internet. In the invention, a rice mutant with a short plant height is obtained when carrying out rice functional genome research by using T-DNA label method; the lateral neighboring sequences of the mutant are researched by using TAIL-PCR technology; meanwhile, the position where the mutant T-DNA inserts the rice genome is arranged on the No. 4 chromosome of the rice by the comparison on the databases of NCBI and TIGR on the Internet; moreover, the rice BAC clone (OSJNBa0084K01) of the position is found out. The T-DNA is inserted between the two genes of the clone by analyzing the clone. Known functional genes with a very high homology with BAC cloning code amino acid sequence are forecasted by the sequence comparison on the Internet.
Owner:TIANJIN AGRICULTURE COLLEGE
Anti-inflammatory peptides and composition comprising the same
ActiveUS9527888B2Improving and preventing skin inflammationAntibacterial agentsAntimycoticsDiseaseAmino acid sequence homology
A peptide with anti-inflammatory activity is described, wherein the peptide comprises at least one amino acid sequence among SEQ ID NO: 2 to SEQ ID NO: 179, the peptide has above 80% homology of amino acid sequence with above-mentioned sequences, or the peptide is the fragment of the above-mentioned peptides. The peptides that have at least one amino acid sequence of SEQ ID NO: 2 to SEQ ID NO: 179 shows outstanding efficacy in both suppressing inflammation and in prophylactic means. Therefore, the composition comprising those peptides can be used as anti-inflammatory pharmaceutical compositions or as cosmetic compositions, in turn, treating and preventing a variety of different types of inflammatory diseases.
Owner:GEMVAX & KAEL
Plant strong salt-resistant gene AtNHXS1 and its coding protein and application
The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence table with the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so as to apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.
Owner:EAST CHINA NORMAL UNIV
Use of interleukin-1receptor antagonist (IL-1Ra), and pharmaceutical compositions of IL-1Ra
InactiveCN102240394ARule out or reduce cardiotoxicityPowder deliveryOrganic active ingredientsChemo therapyBiomedicine
The invention belongs to the technical field of biomedicine, specifically relates to a use of an interleukin-1receptor antagonist (IL-1Ra), and pharmaceutical compositions of the IL-1Ra. The invention discloses a use of protein selected from the following (a) or (b) in preparing drugs for treating or preventing a cardiac toxic reaction due to chemotherapeutics: (a) amino acid sequence of the protein is represented by SEQ ID NO.1; (b) the protein shares 70% amino acid sequence identity with the protein provided by the (a), and has activities for treating or preventing the cardiac toxic reaction due to the chemotherapeutics. According to the protein provided by the present invention, the cardiac toxic reaction due to the chemotherapeutics can be effectively removed or reduced, and a new selectivity may be provided for the future clinical treatment of tumor.
Owner:SHANGHAI JIAO TONG UNIV +1
Novel fish odinagogue
InactiveCN101947308AEffectively induces ovulation activityHave the effect of inducing laborPeptide/protein ingredientsSexual disorderZoologyAmino acid sequence homology
The invention discloses a novel fish odinagogue, which is a polypeptide having an amino acid sequence of Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr or a polypeptide having an amino acid sequence which has an over 60-percent homology with the amino acid sequence of Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr. When use, the polypeptide is dissolved in normal saline for fish according to a ratio of 1 milligram peptide per milliliter of normal saline; the injection amount is 0.5 to 1mu g per gram of fish; and the solution is injected into the fish at a position near the base part of the pectoral fin or the muscle of the dorsal fin. The novel fish odinagogue can induce the ovulation of goldfish, crucian and loach, and is novel fish odinagogue with an important production prospect.
Owner:SUN YAT SEN UNIV
Rabies virus recombinant antigen and preparation method and application thereof
ActiveCN111471112AGood antigenicityImprove featuresSsRNA viruses negative-senseAntibody mimetics/scaffoldsAntigen epitopeVirus antigen
The invention relates to the technical field of rabies application, in particular to a rabies virus recombinant antigen and a preparation method and application thereof. The rabies virus recombinant antigen comprises one of the following parts (a) to (c): (a) amino acid sequence fragments N, G and P are sequentially included in the direction from an end N to an end C; (b) the rabies virus recombinant antigen is obtained by substituting, deleting or adding at least one or more amino acids to the amino acid sequence fragments N, G and / or P in (a), and an immune reaction of mammals to the rabiesvirus recombinant antigen can be induced; or (c) an amino acid sequence having homology with the amino acid sequence of the rabies virus recombinant antigen in (a) is obtained, and an immune reactionof the mammals to the rabies virus recombinant antigen can be induced. The rabies virus recombinant antigen has an antigen epitope with good antigenicity and small non-specificity. Compared with a whole-virus antigen, the rabies virus recombinant antigen has the advantages of stability, safety, low culture cost and high efficiency.
Owner:北京世纪元亨动物防疫技术有限公司
A kind of l-threonine aldolase and its application in the synthesis of p-thymphenylphenylserine
ActiveCN110577948BShort reaction timeBacteriaMicroorganism based processesGenetic engineeringBiology
The invention provides L-threonine aldolase. The L-threonine aldolase has the activity of asymmetric catalytic synthesis of (2S,3R)-methylsulfonylphenylserine from methylsulfonyl benzaldehyde and glycine. The L-threonine aldolase is selected from any one of the following groups: (1) polypeptide with the amino acid sequence shown in SEQ ID NO: 1; (2) polypeptide with the amino acid sequence shown in SEQ ID NO: 1, wherein the homology of the amino acid sequence is greater than or equal to 80%; and (3) derived polypeptide formed by substituting, missing or adding of 1-5 amino acid residues of theamino acid sequence shown in SEQ ID NO: 1 and retaining catalytic activity. The invention further provides application of the L-threonine aldolase to synthesis of the methylsulfonylphenylserine, an L-threonine aldolase gene derived from Actinocorallia herbida is mined and screened, the L-threonine aldolase derived from recombinant escherichia coli is expressed through a gene engineering technology, the (2S,3R)-methylsulfonylphenylserine is subjected to asymmetric catalytic synthesis by taking the methylsulfonyl benzaldehyde and the glycine as raw materials and adopting a whole-cell catalysismethod, and the reaction conditions are mild and environmentally friendly.
Owner:福建昌生生物科技发展有限公司
New lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape
The invention discloses a new lycine aldehyde dehydrogenase gene BnBADH-1 in cabbage type rape. The nucleotide sequence is as shown in SEQ ID NO:1; and the coded amino acid sequence is as shown in SEQ ID NO:2. A new BADH gene sequence is obtained by cloning in cabbage type rape and is named as BnBADH-1. Compared with Arabidopsis thaliana BADH 1 (ALDH10A8), the BnBADH-1 has a homology of 90.24% in nucleic acid sequence and a homology of 93.41% in amino acid sequence. Compared with another rape BADH (AY351634) existing in GeneBank, the BnBADH-1 has a homology of 75.79% in nucleic acid sequence and a homology of 77.93% in amino acid sequence. Thus, the BnBADH-1 is a new gene different from the AY351634.
Owner:CROP INST SICHUAN PROVINCE ACAD OF AGRI SCI
Enzyme co-expression system and its application in the synthesis of sialic acid
ActiveCN114507658BHigh expressionImprove expression levelOrganic active ingredientsCosmetic preparationsDual promoterSynexpression
The present invention relates to the field of biochemistry, in particular to an enzyme co-expression system and its application in synthesizing sialic acid. The present invention provides an N-acetylneuraminic acid aldolase (NAL) mutant having the amino acid sequence shown in SEQ ID NO: 1; or a substitution, deletion, addition and / or substitution 1 on the basis of the above amino acid sequence A sequence of one or more amino acids; or a sequence with more than 90% homology to the above amino acid sequence. The dual-promoter co-expression system adopted in the present invention simultaneously adopts the molecular biological means of increasing expression, realizes the simultaneous overexpression of two enzymes, and realizes the preparation of enzyme liquid by one-time sterilization process, thereby improving the reaction efficiency of the enzyme-catalyzed reaction. . At the same time, for Clostridium Fusarium ( Clostridium scindens ) NAL was modified to improve the synthesis efficiency of the forward reaction to produce sialic acid. The yield of the enzyme-catalyzed process in the present invention meets the requirements of industrial production.
Owner:SHENZHEN READLINE BIOTECH CO LTD
Pig tyraminase beta protein coding sequence
Hog monoamine oxidase B protein coding sequence is characterized by having nucleotide sequence from 129-1691 bit in SEQ IDNO.1 and monoamine oxidase B protein coding sequence homology 92.73% with human, rat, ox and dog, having amino acid sequence polypeptide in SEQ ID No:1, or its conserved variant polypeptide, or its active fragment, or its active derivative, having monoamine oxidase B amino acid sequence homology 93.81% with human, rat, ox and dog, cloning according MAO-B gene sequence, sequencing, obtaining hog MOA-B cDNA sequence, and homologous comparing for different species CDS sequence of MAO-B gene. It can be used for relation of MAO-gene and hog stress sensitivity and human related stress diseases.
Owner:SHANGHAI JIAO TONG UNIV
Construction, production method and application of high-performance starch debranching enzyme chimera strain
InactiveCN113667662AGood application effectGood pH toleranceBacteriaAntibody mimetics/scaffoldsAmino acid sequence homologyBacillus acidopullulyticus
The invention discloses construction, a production method and application of of a high-performance starch debranching enzyme chimera strain. A high-performance starch debranching enzyme chimera is obtained by effective chimeric combination of two parental pullulanase amino acid sequences, and the homology of the high-performance starch debranching enzyme chimera and the parental pullulanase amino acid sequences is not less than 95%; and the high-performance starch debranching enzyme chimera is obtained by carrying out chimeric combination on amino acid residues from site 1 to site 476 at the tail end of parental pullulanase of bacillus acidopullulyticus and amino acid residues from site 465 to site 976 at the tail end of parental pullulanase of bacillus deramificans. The chimeric pullulanase has a relatively high saccharification rate, also has higher heat resistance and thermal stability than the parental pullulanase, and is suitable for a saccharification reaction and starch liquefaction.
Owner:南京纽田科技有限公司
Juvenile hormone transmethylase genes and method of using the same
Juvenile hormone acid methyltransferase gene was cloned using a cDNA derived from the corpora allata of Bombyx mori by differential display methods. A recombinant protein expressed in Escherichia coli, which was transformed with a vector DNA incorporating the gene, was found to have juvenile hormone acid methyltransferase activity. Based on amino acid sequence homology, juvenile hormone acid methyltransferase genes were found from Drosophila melanogaster, Anopheles gambiae, Spodoptera litura, and Helicoverpa armigera. The proteins encoded by the juvenile hormone acid methyltransferase genes derived from Drosophila melanogaster, Spodoptera litura, and Helicoverpa armigera were found to have juvenile hormone acid methyltransferase activities.
Owner:INC ADMINISTRATIVE AGENCY NAT AGRI & BIO ORIENTED RES ORG
Recombinant protein for resisting porcine pseudorabies virus reproduction and application thereof
InactiveCN103739697AEnhanced inhibitory effectBacteriaPeptide/protein ingredientsAmino acid sequence homologyFunctional protein
The invention discloses a recombinant protein for resisting porcine pseudorabies virus reproduction, and application of the recombinant protection to synthesis of a medicine for resisting porcine pseudorabies virus reproduction. The recombinant protein for resisting porcine pseudorabies virus reproduction is a protein with an amino acid sequence consisting of an amino acid sequence shown as SEQ ID No.2, a protein with an amino acid sequence which has homology of 95-100 percent with the amino acid sequence limited by the sequence SEQ ID No.2, and is used for coding proteins with same functions, or a protein which is derived from the amino acid sequence shown as SEQ ID No.2 through addition, deletion or substitution of one or more amino acids and has equal activity. The recombinant protein provided by the invention is used in the medicine for resisting the porcine pseudorabies virus reproduction; experiments prove that the recombinant protein has a remarkable inhibition effect.
Owner:HUBEI UNIV
Method of screening anti-mycobacterial molecules
This invention relates to a novel mycobacterial protein named DES, which appears to share significant amino acid sequence homology with soluble stearoyl-ACP desaturases. The results of allelic exchange experiments, indicate that the des gene may be essential to the survival of mycobacteria. These results coupled with the surface localization, the unique structure of DES, and the fact this antigen is expressed in vivo, and DES protein induces a humoral response in human patients, indicate that the DES protein provides a new target for the design of anti-mycobacterial drugs. This invention provides methods of screening molecules that can inhibit the DES enzyme activity of purified DES protein, in order to identify antibiotic molecules that are capable of inhibiting the growth or survival of mycobacteria. These methods may be practiced by using recombinant DES protein obtained from a recombinant mycobacterium host cell that was transformed with a vector containing the des gene, whose expression is controlled by regulatory or promoter sequences that function in mycobacteria. Another aspect of this invention relates to the molecules that have been identified according to the screening methods as having antibiotic activity against mycobacteria.
Owner:JACKSON MARY +1
Protein encoding sequence of pig nuclear receptor coactivity factor 1 gene
The invention relates to a protein encoding sequence of pig nuclear receptor coactivity factor 1 gene, which includes No.1-4323 nucleotide sequence in SEQ ID NO.1, with the human, rat species NCOA-1 / SRC-1 gene CDS sequence consanguinity being 93.73%, it has polypeptide of the amino acid sequence represented by SEQ ID NO.1, or its conservative variation polypeptide, of its active segments, or its active derivative, with the human, rat and mouse amino acid sequence consanguinity being 94.99%. The invention can be applied for providing animal models for the research of human mammary cancer, diabetes, obesity and related diseases.
Owner:SHANGHAI JIAO TONG UNIV
Avian adenovirus hexon and chicken infectious bursal disease virus vp2 fusion antigen, subunit vaccine and preparation method thereof
ActiveCN107488234BReduce repetitive workImprove labor efficiencyViral antigen ingredientsAntibody mimetics/scaffoldsProtective antigenDisease
The invention discloses a fusion antigen of avian adenovirus Hexon and chicken infectious bursal disease virus VP2, a subunit vaccine, a preparation method and application thereof. The invention relates to a fusion antigen of avian adenovirus Hexon and chicken infectious bursal disease virus VP2, wherein the fusion antigen includes avian adenovirus Hexon protein and chicken infectious bursal disease virus VP2 protein. The fusion antigen has the following amino acid sequence: (1) a protein consisting of the amino acid sequence shown in SEQ ID No.1; or (2) a homology of 95% to 100 to the amino acid sequence defined by SEQ ID No.1 % amino acid sequence encoding the same functional protein. The protective antigen of the subunit vaccine prepared by the invention is a fusion antigen, which is produced by genetic engineering fermentation, and has the advantages of low cost, high antigen purity, good immunogenicity and high safety.
Owner:RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
A transcription factor that enhances plant stress resistance
ActiveCN109971771BReduce sensitivityImprove salt tolerancePlant peptidesFermentationBiotechnologyNucleotide
Owner:GRASSLAND RES INST OF XINJIANG ACAD OF ANIMAL SCI
Application of rpgmp22, a gametocyte recombinant protein of Plasmodium berghei, in blocking malaria transmission
InactiveCN107056920BImprove transmission blocking effectEase of useDepsipeptidesAntiparasitic agentsEngineeringAmino acid
The invention belongs to the field of immunology and specifically relates to a recombinant protein (rPgmp22) of a gametophyte of plasmodium berghei as well as a preparation method and application of the recombinant protein (rPgmp22) to interruption of malaria transmission. The amino acid sequence of the recombinant protein (rPgmp22) of the gametophyte of the plasmodium berghei is SEQ ID NO: 1; the recombinant protein (rPgmp22) is an amino acid sequence with 95-100% of homology with the amino acid sequence limited by SEQ ID NO: 1, which is used for coding a protein with same function; or the recombinant protein (rPgmp22) is a derived pretein with the same activity, which is formed by increasing, deleting or replacing one or a plurality of amino acids for the amino acid sequence shown by SEQ ID NO: 1. The recombinant protein is a novel recombinant protein with a transmission interruption effect, so that the malaria transmission interruption effect can be further improved.
Owner:中国医科大学
Application of a gametocyte recombinant protein rpbg37 of Plasmodium berghei in blocking malaria transmission
InactiveCN107176991BImprove transmission blocking effectEase of useDepsipeptidesAntiparasitic agentsAmino acidProtein
The invention belongs to the field of immunology and particularly relates to Plasmodium berghei gametophyte recombinant protein (rPbG37), a preparation method thereof and application of the Plasmodium berghei gametophyte recombinant protein (rPbG37) in malaria transmission blocking. The amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is as shown in SEQ ID No. 1; or the amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is an amino acid sequence whose homology with an amino acid sequence defined by the sequence as shown in SEQ ID No. 1 is 95-100% and encoding identical functional protein; or the amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is an amino acid sequence obtained by increasing, deleting or replacing one or more amino acid of the amino acid sequence as shown in SEQ ID No. 1 and provided with identical-activity derivative protein. The recombinant protein is novel recombinant protein with a transmission blocking effect and can further increase a malaria transmission blocking effect.
Owner:中国医科大学
Plasmodium berghei gametophyte recombinant protein (rPbG37), preparation method thereof and application of Plasmodium berghei gametophyte recombinant protein (rPbG37) in malaria transmission blocking
InactiveCN107176991AImprove transmission blocking effectEase of useDepsipeptidesAntiparasitic agentsADAMTS ProteinsImmunologic function
The invention belongs to the field of immunology and particularly relates to Plasmodium berghei gametophyte recombinant protein (rPbG37), a preparation method thereof and application of the Plasmodium berghei gametophyte recombinant protein (rPbG37) in malaria transmission blocking. The amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is as shown in SEQ ID No. 1; or the amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is an amino acid sequence whose homology with an amino acid sequence defined by the sequence as shown in SEQ ID No. 1 is 95-100% and encoding identical functional protein; or the amino acid sequence of the Plasmodium berghei gametophyte recombinant protein (rPbG37) is an amino acid sequence obtained by increasing, deleting or replacing one or more amino acid of the amino acid sequence as shown in SEQ ID No. 1 and provided with identical-activity derivative protein. The recombinant protein is novel recombinant protein with a transmission blocking effect and can further increase a malaria transmission blocking effect.
Owner:中国医科大学
Process for producing useful substance
InactiveUS20100047872A1Efficient productionGenetically modified cellsBacteria peptidesAmino acid sequence homologyGene
The present invention provides a process for producing a useful substance by use of a microorganism that lacks in their chromosomal DNA all or part of a gene encoding a protein having the amino acid sequence shown in SEQ ID NO: 1 or a gene encoding a protein having 80% or more, preferably 90% or more, more preferably 95% or more, even more preferably 97% or more, still more preferably 98% or more, and yet more preferably 99% or more homology with the amino acid sequence shown in SEQ ID NO: 1.
Owner:KYOWA HAKKO KIRIN CO LTD
Transcription factor with function of improvement of plant stress resistance
ActiveCN109971771AReduce sensitivityImprove salt tolerancePlant peptidesFermentationNucleotideNucleotide sequencing
The invention belongs to the field of biotechnology and botany and particularly relates to a transcription factor ScABI3 with a function of improvement of plant stress resistance. The transcription factor has a nucleotide sequence shown as SEQ ID NO.3 and other sequences in homology not lower than 51% and further has an amino acid sequence shown as SEQ ID NO.4 and other sequences in homology not lower than 51%. The ScABI3 gene has the function of evident improvement of plant stress resistance.
Owner:GRASSLAND RES INST OF XINJIANG ACAD OF ANIMAL SCI
Kiwi fruit germplasm material resistant to extreme high-temperature growth conditions and cultivation method
PendingCN114250246AReasonable designEasy to operatePlant peptidesGenetic engineeringBiotechnologyNucleotide
The invention provides a kiwifruit germplasm material with strong resistance to an extreme high-temperature growth condition and a cultivation method. The kiwifruit germplasm material is obtained by over-expressing a heat shock transcription factor HsfA2-1. The kiwifruit germplasm material has extremely high resistance in the aspect of high temperature stress resistance, can continuously resist extreme high temperature stress of 50 DEG C for at least 2 hours, and can continuously and normally grow after being stressed. According to the invention, a heat shock transcription factor AcHsfA2-1 in Donghong kiwi fruit is taken as an example, and the cultivation method of the germplasm material is elaborated. The application of other genes, of which the homology with the nucleotide sequence or amino acid sequence of the AcHsfA2-1 is higher than 90%, in the aspect is within the protection range of the invention. The cultivation method is reasonable in design, convenient to operate and easy to popularize and apply.
Owner:ZHEJIANG UNIV
Preparation method of agaricus bisporus heme dioxygenase and application thereof
The invention belongs to the technical field of agaricus bisporus genetic engineering, and particularly relates to agaricus bisporus heme dioxygenase and application thereof. A known amino acid sequence of fungal heme dioxygenase is utilized, comparing with an agaricus bisporus genome sequence,and according to the agaricus bisporus heme dioxygenase, the heme dioxygenase of agaricus bisporus is obtained, the heme dioxygenase has a CYP domain. The amino acid sequence homology with the PpoC of aspergillus nidulans is 33%, the similarity is 51%, but the CYP domain of the heme dioxygenase is the same as that of the PpoC, a complete P450 characteristic consensus sequence heme conservative binding region is not contained, it is speculated that the CYP domain of the heme dioxygenase possibly has no function, and the heme dioxygenase is named AbDOX-(CYP). The cloning of the gene and the conversion expression of escherichia coli further show that the heme dioxygenase can catalyze linoleic acid to synthesize 1-octene-3-ol. On the basis of the evidence, a good technical foundation can be laid for further industrial production improvement of the 1-octene-3-ol.
Owner:HENAN AGRICULTURAL UNIVERSITY
Application of a l-threonine transaldolase in the synthesis of florfenicol chiral intermediates
ActiveCN110540977BIncrease added valueSave separation and purificationTransferasesFermentationEscherichia coliBenzaldehyde
Owner:福建昌生生物科技发展有限公司
Alcohol dehydrogenase mutant and its application
ActiveCN104531628BLow costHigh stereoselectivityBacteriaOxidoreductasesMutantAmino acid sequence homology
The invention discloses an alcohol dehydrogenase mutant and application thereof. The amino acid sequence of the alcohol dehydrogenase mutant is an amino acid sequence obtained by mutating an amino acid sequence as shown in SEQ ID NO:9; the mutated amino acid sequence has at least one mutation site selected from the following mutation sites: the 40th position, the 87th position, the 194th position and the 331st position; T on the 40th position is mutated into S, A or C; W on the 87th position is mutated into F, Y or H; V on the 194th position is mutated into I, L or E; R on the 331st position is mutated into A, K or M; or the amino acid sequence of the alcohol dehydrogenase mutant has the mutation sites in the mutated amino acid sequence, and the alcohol dehydrogenase mutant contains more than 90% of amino acid sequences which are homologous with the mutated amino acid sequence. The stereoselectivity and enzyme activity of the alcohol dehydrogenase mutant which is provided with the at least one mutation site or retains the mutation site and contains more than 90% of amino acid sequences which are homologous with the mutated amino acid sequence are greatly improved.
Owner:ASYMCHEM LAB TIANJIN +5
A kind of rabies virus recombinant antigen and preparation method and use thereof
ActiveCN111471112BGood antigenicityImprove featuresSsRNA viruses negative-senseSerum immunoglobulinsEpitopeAmino acid sequence homology
The present invention relates to the technical field of rabies application, in particular to a rabies virus recombinant antigen and a preparation method and use thereof, including one of the following (a)-(c): (a), in the direction from the N-terminal to the C-terminal, including Such as amino acid sequence fragments N, G and P; (b), amino acid sequence fragments N, G and / or P in (a) have been substituted, deleted or added at least one or several amino acid recombinant antigens of rabies virus, and can be Inducing an immune response in a mammal against the rabies virus recombinant antigen; or (c), an amino acid sequence having homology to the amino acid sequence of the rabies virus recombinant antigen in (a), and eliciting a mammal against the rabies virus Immune response of recombinant antigens; the above-mentioned rabies virus recombinant antigens have antigenic epitopes with good antigenicity and non-specificity, and have the advantages of stability, safety, low culture cost and high efficiency compared with holovirus antigens.
Owner:北京世纪元亨动物防疫技术有限公司
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