37 results about "Amino acid sequence homology" patented technology

Provided in the present invention are recombinant peptides and a method for using the peptides in stimulating an immune response against human high molecular weight-melanoma associated antigen (HMW-MAA). The peptides were designed from the identification of regions of structural and amino acid sequence homology between HMW-MAA and the mouse anti-idiotypic monoclonal antibody MK2-23. The method comprises the step of administering to an individual a peptide of the invention in an amount effective to elicit an immune response against HMW-MAA.

The invention provides L-threonine aldolase. The L-threonine aldolase has the activity of asymmetric catalytic synthesis of (2S,3R)-methylsulfonylphenylserine from methylsulfonyl benzaldehyde and glycine. The L-threonine aldolase is selected from any one of the following groups: (1) polypeptide with the amino acid sequence shown in SEQ ID NO: 1; (2) polypeptide with the amino acid sequence shown in SEQ ID NO: 1, wherein the homology of the amino acid sequence is greater than or equal to 80%; and (3) derived polypeptide formed by substituting, missing or adding of 1-5 amino acid residues of theamino acid sequence shown in SEQ ID NO: 1 and retaining catalytic activity. The invention further provides application of the L-threonine aldolase to synthesis of the methylsulfonylphenylserine, an L-threonine aldolase gene derived from Actinocorallia herbida is mined and screened, the L-threonine aldolase derived from recombinant escherichia coli is expressed through a gene engineering technology, the (2S,3R)-methylsulfonylphenylserine is subjected to asymmetric catalytic synthesis by taking the methylsulfonyl benzaldehyde and the glycine as raw materials and adopting a whole-cell catalysismethod, and the reaction conditions are mild and environmentally friendly.

The invention belongs to the fields of biotechnology and botany; in particular, it relates to a transcription factor for improving stress resistance of plants ScABI3 , having the nucleotide sequence shown in SEQ ID NO.3, other sequences with a homology of not less than 51%; and the amino acid sequence shown in SEQ ID NO.4, other sequences with a homology of not less than 51%. ScABI3 The gene has the function of obviously improving the plant's resistance to adversity stress.

The invention belongs to the field of immunology and specifically relates to a recombinant protein (rPgmp22) of a gametophyte of plasmodium berghei as well as a preparation method and application of the recombinant protein (rPgmp22) to interruption of malaria transmission. The amino acid sequence of the recombinant protein (rPgmp22) of the gametophyte of the plasmodium berghei is SEQ ID NO: 1; the recombinant protein (rPgmp22) is an amino acid sequence with 95-100% of homology with the amino acid sequence limited by SEQ ID NO: 1, which is used for coding a protein with same function; or the recombinant protein (rPgmp22) is a derived pretein with the same activity, which is formed by increasing, deleting or replacing one or a plurality of amino acids for the amino acid sequence shown by SEQ ID NO: 1. The recombinant protein is a novel recombinant protein with a transmission interruption effect, so that the malaria transmission interruption effect can be further improved.

The invention provides application of L-threonine transaldolase to synthesis of florfenicol chiral intermediates. The L-threonine transaldolase is selected from any one of the following groups that (1) polypeptide contains an amino acid sequence shown in SEQ ID NO:1; (2) polypeptide contains an amino acid sequence greater than or equal to 90% homology shown in SEQ ID NO:1, and the polypeptide hascatalytic activity; and (3) derived polypeptide is formed by substitution, deletion or addition of 1-5 amino acid residues to the amino acid sequence shown in SEQ ID NO:1 and with the retention of catalytic activity. According to the application, new L-threonine transaldolase genes are screened through gene mining, the L-threonine transaldolase derived from recombinant escherichia coli is expressed by adopting a genetic engineering method, methylsulfonyl benzaldehyde and the L-threonine transaldolase are used as raw materials, (2S,3R)-methylsulfonyl phenylserine is synthesized through a wholecell catalyzed reaction, fluorfenicol key chiral synthesis blocks can be obtained by a one-step reaction under the room temperature and pressure, environmental protection is achieved, and the application is an environment-friendly biosynthetic pathway.

The invention discloses an alcohol dehydrogenase mutant and application thereof. The amino acid sequence of the alcohol dehydrogenase mutant is an amino acid sequence obtained by mutating an amino acid sequence as shown in SEQ ID NO:9; the mutated amino acid sequence has at least one mutation site selected from the following mutation sites: the 40th position, the 87th position, the 194th position and the 331st position; T on the 40th position is mutated into S, A or C; W on the 87th position is mutated into F, Y or H; V on the 194th position is mutated into I, L or E; R on the 331st position is mutated into A, K or M; or the amino acid sequence of the alcohol dehydrogenase mutant has the mutation sites in the mutated amino acid sequence, and the alcohol dehydrogenase mutant contains more than 90% of amino acid sequences which are homologous with the mutated amino acid sequence. The stereoselectivity and enzyme activity of the alcohol dehydrogenase mutant which is provided with the at least one mutation site or retains the mutation site and contains more than 90% of amino acid sequences which are homologous with the mutated amino acid sequence are greatly improved.