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Transcription factor with function of improvement of plant stress resistance

A plant stress resistance, transcription factor technology, applied in the fields of biotechnology and botany

Active Publication Date: 2019-07-05
GRASSLAND RES INST OF XINJIANG ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular regulation mechanism of ABA has been studied more in vascular plants, but in the early landed bryophytes, there are only reports that ABI3 regulates the desiccation tolerance of Physcomitrella patens plants and improves the frost resistance of mosses. played a key role in the process; however, the mechanism of ABA regulation in extreme dessication tolerance moss has not been reported yet.

Method used

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  • Transcription factor with function of improvement of plant stress resistance
  • Transcription factor with function of improvement of plant stress resistance
  • Transcription factor with function of improvement of plant stress resistance

Examples

Experimental program
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Embodiment 1

[0014] Example 1. Cloning and analysis of the ABI3 gene of Erythros dentatus Erythros dentition, an extremely dry-tolerant moss widely distributed in the Gurbantunggut Desert, was used as the research material.

[0015] Experimental material Plant material: After the Erythra denticola collected in the field was completely rehydrated, the gametophyte was selected as the material.

[0016] Strains and vectors: Escherichia coli DH5α was purchased from Beijing Quanshijin Biological Company, and the pMD19-T cloning vector was purchased from TaKaRa Company.

[0017] Drugs and reagents: RNA extraction kit, agarose gel recovery kit, plasmid extraction kit, PCR product purification kit were purchased from OMEGA Company, reverse transcription kit, Ex Taq high-fidelity polymerase were purchased from TaKaRa Company, α -Galactosidase chromogenic substrate was purchased from Solarbio Company, primers were synthesized by Suzhou Jinweizhi Company, and DNA sequencing was completed by Huada Gen...

Embodiment 2

[0053] Example 2. Transformation of Arabidopsis thaliana with the ScABI3 gene of Erythra dentata and verification of stress resistance function

[0054] Transgenic Arabidopsis thaliana ScABI3 homozygous seeds were obtained through routine biological operations, and then stress resistance analysis of transgenic Arabidopsis thaliana under abiotic stress

[0055] (1) Analysis of ScABI3 gene expression in transgenic Arabidopsis

[0056] Semi-quantitative RT-PCR was performed on the T3 homozygous lines of ScABI3 transgenic Arabidopsis, using α-tublin as an internal reference gene. The expression of ScABI3 gene in transgenic Arabidopsis lines was analyzed by amplifying the brightness of the bands. Subsequently, among the 11 transgenic lines, No. 4 and No. 8 with a relatively middle expression level and No. 11 with a relatively high expression level were selected for subsequent experimental research and named as Line1, Line2 and Line3 respectively, such as figure 2 shown.

[0057...

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Abstract

The invention belongs to the field of biotechnology and botany and particularly relates to a transcription factor ScABI3 with a function of improvement of plant stress resistance. The transcription factor has a nucleotide sequence shown as SEQ ID NO.3 and other sequences in homology not lower than 51% and further has an amino acid sequence shown as SEQ ID NO.4 and other sequences in homology not lower than 51%. The ScABI3 gene has the function of evident improvement of plant stress resistance.

Description

technical field [0001] The invention belongs to the fields of biotechnology and botany; in particular, it relates to a transcription factor for improving stress resistance of plants. Background technique [0002] Abscisic acid (ABA) is a key phytohormone in response to stress and signal transduction in vascular plants under water stress. It can regulate plant transpiration, drought tolerance, seed maturation, and dormancy. It also inhibits lateral root and inflorescence formation. The biosynthesis of ABA in higher plants is mainly synthesized from carotenoids through the C40 indirect pathway, including zeaxanthine cyclooxygenase (zeaxanthinepoxidase, ZEP) catalyzing the conversion of zeaxanthin (zeaxanthin) into xanthin (violaxanthin), 9-cis-epoxy Carotenoid dioxygenase (9-cis-epoxycarotenoid dioxygenase, NCED) catalyzes 9-cis-neoxanthin (9-cis-neoxanthin) to produce xanthoxin (xanthoxin). The hydrogenase (short-chain alcohol dehydrogenase) ABA2 is converted into abscisic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C07K14/415
CPCC07K14/415
Inventor 张一弓张道远张荟荟李学森刘梦柯梅张学洲兰吉勇史伟
Owner GRASSLAND RES INST OF XINJIANG ACAD OF ANIMAL SCI
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