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Protein encoding sequence of pig nuclear receptor coactivity factor 1 gene

A coding sequence and activating factor technology, applied in the field of protein coding sequence, can solve the problems such as the undiscovered cloning of pig NCOA

Inactive Publication Date: 2005-11-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no clone has been found to obtain the coding protein sequence of pig NCOA-1 / SRC-1 gene

Method used

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  • Protein encoding sequence of pig nuclear receptor coactivity factor 1 gene

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Cloning of pig NCOA-1 gene cDNA sequence, the process is shown in the appendix figure 1 .

[0022] The specific operation method of the experimental method used in the gene cloning process is as follows:

[0023] 1. Isolation

[0024] The 30-day-old Dudu piglets were slaughtered in the pig farm, and the colon tissue samples were taken, quickly put into liquid nitrogen, frozen and stored in the -80°C refrigerator in the laboratory, ready to extract total RNA from the tissue.

[0025] 2. Total RNA isolation (Total RNA isolation)

[0026] (1) Preparation for RNA extraction

[0027]Glass products were soaked in 0.1M NaOH overnight, rinsed repeatedly with tap water, rinsed twice with distilled water, and baked at 180°C for 4 hours.

[0028] Soak the homogenizer and electrophoresis tank with 3% hydrogen peroxide for 20-30 minutes, and then rinse with 0.1% DEPC water. Since the uninactivated DEPC will affect the PCR reaction, it can be treated with 0.5% SDS.

[0029] Soak...

Embodiment 2

[0068] Homology comparison of coding sequence of porcine NCOC-1 / SRC-1 gene

[0069] Search and obtain human, mouse, and rat NCOA-1 gene coding protein sequences on GenBank, and perform sequence homology on the molecular biology software DNAman together with the SEQ ID NO.1 sequence obtained by cloning and sequencing of the present invention Compare. The comparison results showed that the homology of SEQ ID NO.1 sequence with NCOA-1 gene CDS sequence of human, mouse and other species was 93.73%, and the homology of amino acid sequence with human, mouse and rat was 94.99%.

[0070] The sequences and symbols involved in the present invention are listed as follows:

[0071] Information on SEQ ID NO.1

[0072] Shanghai Jiaotong University

[0073] Protein coding sequence of porcine nuclear receptor coactivator 1 gene

[0074]

[0075] 2005-05-18

[0076] 2

[0077] PatentIn version 3.1

[0078] 1

[0079] 4323

[0080] DNA

[0081] pig (sus scrofa)

[0082] 1

[00...

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Abstract

The invention relates to a protein encoding sequence of pig nuclear receptor coactivity factor 1 gene, which includes No.1-4323 nucleotide sequence in SEQ ID NO.1, with the human, rat species NCOA-1 / SRC-1 gene CDS sequence consanguinity being 93.73%, it has polypeptide of the amino acid sequence represented by SEQ ID NO.1, or its conservative variation polypeptide, of its active segments, or its active derivative, with the human, rat and mouse amino acid sequence consanguinity being 94.99%. The invention can be applied for providing animal models for the research of human mammary cancer, diabetes, obesity and related diseases.

Description

technical field [0001] The invention relates to a protein coding sequence used in the field of biogenetic engineering, in particular to a protein coding sequence of porcine nuclear receptor coactivator 1 gene (NCOA-1 / SRC-1). Background technique [0002] Nuclear receptor coactivator 1 (NCOA-A / SRC-1) is a major member of the steroid receptor coactivator (SRC) family. Proteins of the SRC family can enhance the transcriptional activation function of these nuclear receptor genes by interacting with nuclear receptors. Because they are easily assembled into a stable pre-initiation complex by basic transcription factors, they can activate gene expression and play a major regulatory role in the process of nuclear receptor hormone gene expression. [0003] Nuclear hormone receptors are ligand-dependent transcription factors that primarily regulate the transcriptional activity of genes involved in biological processes such as development, reproduction, and homeostasis. These recepto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12
Inventor 潘玉春于丹丹白春艳王起山张令刚
Owner SHANGHAI JIAO TONG UNIV
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