105 results about "Alpha-lactalbumin" patented technology

The invention discloses a milk allergen test plate, which belongs to the gold immunochromatography detection. According to the milk allergen test plate disclosed by the invention, two ends of a PVC (polrvinyl chloride) base plate are respectively provided with a to-be-tested sample zone and an absorption zone. Colloidal gold mark antigens prepared by respectively marking colloidal gold into casein, beta lactoglobulin and alpha lactalbumin and mixing are orderly arranged between the sample zone to be tested and the absorption zone, and a nitrocellulose membrane is respectively provided with a detection zone coated with mixed milk allergen and a quality control zone coated with anti-beta lactoglobulin antibodies. In detection, color lines are formed in the detection zone and the quality contol zone when an immune complex is formed by specific milk antibodies contained in samples. If the samples do not contain specific milk antibodies, the detection zone does not display color, and only one color line is formed in the quality control zone. The milk allergen test plate disclosed by the invention with the design has the advantages of strong pertinence to the allergen detection, simplicity in operation, low cost and high sensitivity and the like, and can prevent the phenomenon of missed diagnosis in the single antibody detection. The milk allergen test plate is applied for rapidly screening patient allergic to milk, and is especially suitable for being used by primary medical treatment units.

The invention discloses a formula milk powder for promoting the absorption of fatty acid and calcium and a preparation method thereof. Raw cow milk, lactose, 1,3-Dioleoyl 2-palmitoyl triglyceride and demineralized whey powder as main materials are added with concentrated whey albumen powder, Alpha-lactalbumin powder, oligosaccharide, walnut oil, casein phosphopeptide, docosahexaenoic acid, arachidonic acid, nucleotide, lutein, inositol, carnitine and the like as well as vitamins, mineral substances and other nutrients needed for strengthening infants, and fat humanization, protein humanization and carbohydrate humanization are realized. The powdery product is produced by the processes of blending, homogenization, concentration, spray-drying, packaging and the like. According to the physiological characteristics and nutritional demand of the infants, the invention reinforces the calcium, the 1,3-Dioleoyl 2-palmitoyl triglyceride, other nutrient ingredients and the like, and aiming at the oversea clinical test conclusion of the 1,3-Dioleoyl 2-palmitoyl triglyceride, the final test conclusion of comparison with breast milk and infant formula milk powder sold on the market in the process of a clinical feeding test is that the feeding result of the designed formula approximates the feeding result of the breast milk and is better than the feeding result of an infant formula milk powder group sold on the market.

The invention provides an infant milk powder with adjusted structure of aliphatic acid, which comprises the following raw materials (by weight portion in tons): dry matter of fresh milk 276, desalinized whey powder 450, structured fatty oil 105, lactose 50, fructooligosaccharide 20, alpha-lactalbumin 10, docosahexaenoic acid 5, arachidonic acid 6, nucleic acid 0. 42, taurine 0. 45, choline 0. 42, L-carnitine 0. 06, beta-carotene 0. 012, composite vitamin 2. 5, composite microelement 3. 5 and balancing refined vegetable oil.

The invention relates to a quantitative detection method for thermal-denaturation and non-denaturation bovine alpha-lactalbumin in milk and milk products by applying an enzymolysis-liquid chromatography and mass spectrometry combination technology. The quantitative detection method comprises the steps as follows: taking a certain amount of milk or milk samples, dissolving and diluting the milk or milk samples in water to obtain solution with total protein content being about 1mg / mL; after volume metering, correctly sucking 500 mu L, adding an internal standard substance, reacting disulfide bond with dithiothreitol (DTT), alkylating to protect sulfydryl produced in reaction by iodoacetamide (IAA), and then conducting constant-temperature and constant-time enzymolysis with trypsin; and separating enzymolysis products by reversed phase liquid chromatography, conducting detection with a mass spectrum multiple reaction monitoring (MRM) manner, and calculating the result by an internal standard method. The quantitation limit of the method is 0.001g / 100g; when adding amount is 0.2, 1.7 and 5.0g / 100g, the recovery rate is 98.9-110.8% (n is equal to 6) and repeatability: RSD (Relative Standard Deviation) is smaller than 7.6%; and the quantitative detection method can be applicable to the quantitative detection of samples with different contents of bovine alpha-lactalbumin.

The invention relates to a high-protein dietary fiber energy protein bar and a production method thereof. The protein bar comprises the following materials in parts by weight: 5-35 parts of soy protein, 0-31 parts of cocoa butter replacer chocolate, 3-29 parts of glucose syrup, 0-18 parts of oligomeric isomaltose, 3-8 parts of concentrated whey protein powder and 3-9 parts of plaint oil. According to the formula of the protein bar, the soy protein, the concentrated whey protein, the glucose syrup and the oligomeric isomaltose are used as main raw materials for simultaneously supplementing exercising people with protein and energy (carbohydrate). The added concentrated whey protein has nutrient substances such as high-quality alpha-lactalbumin, beta-lactoglobulin and branched chain amino acid, thus being beneficial to rapidly supplementing protein after movement and relieving fatigue; the protein, energy (carbohydrate) and functional nutrient substances (such as dietary fiber) are rapidly supplemented to the exercising people; the protein bar provided by the invention is easy to carry, convenient to eat and good in taste.

The invention discloses infant formula goat milk powder and a preparation method thereof. Alpha-lactalbumin and hydrolytic lactalbumin peptide, malt sugar (corn syrup), glucose, pure colostrum powder, lactoferrin, living bifidobacterium and the like are added in the infant formula goat milk powder. The method comprises the steps of: obtaining goat milk, performing pasteurization (85 DEG C), cutting material mixing tanks with high speed (the material in the tank includes malt sugar, glucose, decavitamin, mineral nutrient, hydrolytic lactalbumin peptide and plant oil), using a homogenizer, performing pasteurization, vacuum concentration and spray drying, cooling a fluid bed (adding the lactoferrin, the pure colostrum powder IgG and the plant oil), mixing the added materials (adding the lactoferrin, the pure colostrum powder IgG and the living bifidobacterium), and canning. The infant formula goat milk powder is easy to digest and absorb, and muting sensitive; and the formula powder is more humanized, and can enhance organism immunity.

Described are the use of alpha-lactalbumin and alpha-lactalbumin enriched whey protein concentrate as a prebiotic food, food additive or food supplement, as well as methods for improving the prebiotic activity of ready-to-consume food products and such ready-to-consume food products including alpha-lactalbumin. Further, pharmaceutical compositions for the treatment of gastro-enteritis including alpha-lactalbumin as active compound are described.

The present invention provides compositions and formulations comprising alpha-lactalbumin and a carbohydrate of high glycemic index. This invention also provides methods of treating pain, pre-menstrual syndrome, and symptoms associated with the pre-menstrual period and menopause.

The invention relates to infant formula milk powder containing a composition of alpha-lactalbumin and lactoferrin and a preparation method for the infant formula milk powder. The infant formula milk powder comprises the following components: vegetable oil or plant fat powder, fresh milk or dried skim milk, whey powder, lactose or glucose solid syrup, whey protein powder, oligosaccharide, a mineral premix, a vitamin premix, nutrient, and the composition of the alpha-lactalbumin and the lactoferrin, wherein the composition accounts for 0.12 to 6.3 percent of the total mass of the infant formula milk powder. The infant formula milk powder contains a specific amount of alpha-lactalbumin and lactoferrin and is close to breast milk. One small step is made in the progress of breast milk conversion of the infant formula milk powder. By supplementing immune essence which is close to the breast milk in vitro, better effects of effectively improving the resistance and immunity of infants are showed; and the infant formula milk powder can effectively improve the immunity of the crowds, and has the effect of regulating sleep.

The invention discloses a method for separating and detecting whey proteins, which comprises the following steps: pretreating the whey protein; and completely separating bovine alpha-lactalbumin, bovine beta a-lactoglobulin, and bovine beta b-lactoglobulin in a sample by an ultra-performance liquid chromatography column taking 1.7 mu m ethylidene bridged hybrid (BEH) particles as a filler, and performing section scan by a mass analyzer to quantitate the unmodified bovine lactalbumin and lactoglobulin. In the method, human alpha-lactalbumin is added as internal standard. The method can accurately measure the contents of the three types of unmodified bovine whey proteins in multiple kinds of food, and has simple pretreatment, high sensitivity, quick detection speed and good selectivity.

The invention discloses a method for quantitatively detecting allergen alpha-lactalbumin based on quantum dot fluorescence and application of the allergen alpha-lactalbumin. The method comprises the following steps of: preparing a monoclonal antibody from alpha-lactalbumin as active immunization BALB / c mice; carrying out conjugation labeling on the alpha-lactalbumin monoclonal antibody by using a fluorescent quantum dot; forming an immunofluorescence complex by adopting a competitive immunosorbent assay; then detecting a fluorescent signal under a full-wavelength multifunctional ELIASA (Enzyme-Linked Immunosorbent Assay Apparatus); and quantitatively detecting the allergen alpha-lactalbumin in the food by establishing a standard curve. The method constructed by the invention can be widely applied to detection of relevant allergens in various powders and liquid milk products, has the characteristics of quickness, accuracy, high sensitivity, favorable repeatability, excellent specificity and the like, provides an effective means for high-throughput detection of relevant allergens in various foods as well as has a favorable popularization and application prospect.

The invention discloses sucrose-free prebiotic biscuits with the function of nursing stomach illness. The sucrose-free prebiotic biscuits comprise the following components: 90 to 110kg of self-raising flour, 30 to 36kg of grease, 5 to 6kg of xylitol, 25 to 35kg of maltitol, 1 to 3kg of alpha-lactalbumin, 4 to 6kg of egg, 2 to 4kg of coix seed, 0.8 to 1.5kg of inulin, 1 to 2kg of seville orange flower, 0.3 to 0.6kg of pearl layer powder, 1 to 2kg of cuttlebone, 0.1 to 0.5kg of baking soda, 0.2 to 0.6kg of ammonia hydrogen carbonate, and 8 to 15kg of water. The medicinal and edible traditional Chinese medicines with remarkable effect of treating the stomach illness, namely the seville orange flower, the cuttlebone and the pearl layer powder for making acid and promoting tissue regeneration are combined to enhance acid making effect, the pain caused by the spasm of stomach and nerves due to gastric acid is relieved through acid making, and the sucrose-free prebiotic biscuits have high response speed. The sucrose-free prebiotic biscuits are packaged in snack bags and can be carried about at any time; and the biscuits are eaten as a snack, so that two purposes of delicious taste and treatment and protection of the stomach are achieved.

The invention relates to the field of analytical chemistry and discloses a method and kit for quantitative detection of content of human alpha-lactalbumin. According to the method disclosed by the invention, a specific polypeptide sequence CELSQLLK obtained after enzymolysis of the human alpha-lactalbumin is utilized to design the sequences of an isotope-labeled specific peptide and an isotope-labeled internal standard substance, a brand new accurate quantitative detection method is provided on the basis of an internal standard method to perform the quantitative detection of the human alpha-lactalbumin, and the accuracy, precision and sensitivity are relatively high.

The invention provides a fermented milk which is excellent in hardness and flavor and a method of producing the same. The method of producing a fermented milk by using a yogurt mix in which 0.3% by weight or more alpha-lactalbumin is added to a starting milk mixture, a yogurt mix containing a whey protein concentrate in which alpha-lactalbumin is contained in an amount of 60% by weight or more based on the protein, a yogurt mix in which 0.4% by weight or more beta-lactoglobulin is added to a starting milk mixture, or a yogurt mix containing a whey protein concentrate in which beta-lactoglobulin is contained in an amount of 65% by weight or more based on the protein.

The invention relates to an enzymatic hydrolysis alpha-lactalbumin polypeptide and an enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle and preparation thereof. Alpha-lactalbumin with a certain purity as a raw material is used for enzymatic hydrolysis in the presence of a particular enzyme to obtain the ideal enzymatic hydrolysis alpha-lactalbumin polypeptide, the enzymatic hydrolysis alpha-lactalbumin polypeptide can be used for preparing the stable particle size enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle, the raw material is low in cost and easy to obtain, and the preparation process is simple; and the micelle is small in particle size, good in dispersion, good in biocompatibility and high in stability, the micelle can be used for embedding of hydrophobic materials, the micelle as a carrier can be made into a supported enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle, and the supported enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle has the advantages of small particle size, good stability, high encapsulation efficiency and non-toxicity.

The invention provides a low-sensitivity glycosylation whey protein hydrolysate and a preparation method thereof. In the preparation method, an enzymolysis method is combined with a glycosylation method, and the content of allergen can be reduced to the greatest degree, wherein enzymes adopted in the enzymolysis method include alkaline protease, neutral protease, papain, trypsin, flavourzyme, protease A and protease M; the sugar adopted in the glycosylation method is one of maltooligosaccharide, glucose and phosphoryl oligosaccharide or a mixture of more. By adopting the method provided by the invention, the antigenicity inhibition ratio on whey protein can be effectively reduced, wherein the antigenicity inhibition ratio on alpha-lactalbumin (alpha-LA) reaches 5%, and the antigenicity inhibition ratio on beta-lactoglobulin (beta-LG) reaches 2%.

The invention provides infant formula milk powder rich in milk fat globule membrane protein and phospholipid. By adding alpha-lactalbumin, milk fat globule membrane and a physiological activator, suchas bifidobacterium animalis Bb-12, milk fat globule membrane lipids and protein, N-acetylneuraminic acid (sialic acid), SN-2 structure lipid, human milk oligosaccharides (HMOs) and human milk oligose(HMO). By simultaneously adjusting the using amount ratio of all the components, especially sphingomyelin, gangliosides, the milk fat globule membrane protein, the human milk oligosaccharides, the human milk oligose, adiponectin and lysozyme, and the proportion between mucin, xanthine oxidoreductase, mucin 15, CD 36, butyrophilin, adipose differentiation-related protein and fatty acid binding proteins, the content of the active ingredients in the milk powder is closer to the content of active ingredients in human milk, the constitution of the infant formula milk powder is optimized, and the formula milk powder obtained by using the method is closer to the human milk.

A long-acting improver of muscular fatigue characterized by comprising 4 kinds of amino acids made up of leucine, isoleucine, valine and glutamine, and a whey protein component (whey protein and / or decomposition product of whey protein). At least one of a whey protein isolate (WPI), a whey protein concentrate (WPC), β-lactoglobulin, and α-lactalbumin is used as the whey protein. Novel food or drink, and pharmaceuticals which exhibit sustained recovery effects on muscular fatigue are provided.

The invention discloses an immunodetection kit for detecting adulterated cow milk in camel milk and an application thereof, wherein alpha-lactalbumin and alphas1-casein in camel milk are used as characteristic protein of the camel milk, and beta-lactoglobulin and kappa casein in cow milk are used as characteristic protein of the cow milk. After the camel milk characteristic protein is separated and purified, the polyclonal antibody and the monoclonal antibody prepared by the commercialized cow milk characteristic protein and the self-made camel milk characteristic protein can be applied to ELISA detection. The kit mainly adopts an indirect ELISA method to qualitatively or quantitatively detect the content of camel-derived alpha-lactalbumin, alphas1-casein or bovine-derived beta-lactoglobulin and kappa-casein in camel milk, the sample pretreatment process is simple, and after a camel milk sample is repeatedly centrifuged to obtain a milk sample, the content can be directly measured after diluted by diluent, and a large number of samples can be simultaneously detected. The determination method is accurate, rapid and convenient, and has wide practicability and development value in thefield of camel milk adulteration monitoring.

The invention provides nucleotide-meal fiber-containing child formula milk powder suitable for children of 1-3 years old and a preparation method thereof. The nucleotide-meal fiber-containing child formula milk powder comprises 16-19g / 100g of total proteins, 1.3-1.4g / 100g of alpha-lactalbumin, 3.3-3.5g / 100g of beta-casein, 18-21g / 100g of fat, 2500-3500mg / 100g of linoleic acid, 0.95-1.2g / 100g of meal fibers, 52-56g / 100g of carbohydrate and 26-58mg / 100g of total nucleotide molecules. The nucleotide-meal fiber-containing child formula milk powder contains greater than or equal to 106CFU / g of active probiotics. The nucleotide-meal fiber-containing child formula milk powder contains composite nucleotides, meal fibers and probiotics and can improve child intestinal tract flora and improve immunity.

The invention discloses a buffalo milk active-bacteria whey beverage and a preparation method thereof. The whey beverage is obtained through the steps of: mixing byproduct whey generated in the preparation process of buffalo milk cheese with fermented buffalo sour milk in a certain ratio, appropriately adding sugar, a stabilizer and spice to obtain a mixture, then concocting, homogenizing and filling the mixture, so as to obtain the buffalo milk active-bacteria whey beverage. The whey beverage has the complete nutritional ingredients of the whey and the sour milk, contains protein, various amino acids, vitamins and mineral substances needed by human bodies, and especially contains various active proteins such as alpha-lactalbumin, beta-lactoglobulin, immune globulin, lactoferrin, lactoperoxidase and active lactobacillus which are contained in the whey; and the beverage is unique in flavor, exquisite in mouth feel, rich in nutrition, and capable of improving the gastrointestinal function and enhancing the immunity.

The present invention relates to a pharmaceutical composition comprising mono- meric alpha-lactalbumin complex, preferably LAC, which is an active complex of alpha-lactalbumin and a fatty acid or lipid with selective cytotoxic activity. The composition of the invention comprises insignificant amounts of oligomeric / multi- meric alpha-lactalbumin complex, preferably LAC. Based on the selective cyto- toxicity of the alpha-lactalbumin complex, preferably LAC composition such compositions are suitable for use in the manufacture of medicaments for use in therapy. Medicaments, comprising monomeric LAC are for use in the treatment of bacterial and viral infections and in particular cancer due to the selective cytotoxic activity. The application further relates to methods of producing a composition comprising monomeric alpha-lactalbumin complex, preferably LAC with cytotoxic activity.

The invention discloses infant formula milk powder which comprises the following components: 15%-45% of dehydrated raw milk, 12%-50% of milk sugar, 0-30% of desalted whey powder, 0-10% of alpha-lactalbumin, 1%-12% of hydrolyzed whey protein, 10%-30% of vegetable oil, 1%-6% of prebiotics, 0.01%-0.2% of choline chloridd, 0.1%-0.5% of calcium carbonate, 0.01%-1% potassium chloride, 0.01%-0.1% taurine, 0.1%-0.5% infant vitamin premix, 0.1%-0.5% infant mineral substance premix, 0.01%-1% of DNA powder, 0.01%-1% of ARA powder and 0.01%-0.1% nucleotide powder. The infant formula milk powder is good in relish, good in powder body sensory evaluation, easy to be digested and absorbed, better in powder quality, and is capable of reducing main anaphylactogen beta-lactoglobulin content.

An infant formula having a relatively high content of triglycerides having palmitic acid in the sn-2 position. The formula may include oligofructose. The formula may also include at least one omega 6 fatty acid and at least one omega 3 fatty acid. The formula may also have a relatively low protein content and an alpha-lactalbumin content similar to human milk The invention also includes a method for improving the stool consistency, increasing bifidobacteria in the colon, reducing potentially pathogenic bacteria in the colon and reducing calcium soaps in the stool of a formula-fed infant.

The invention relates to zinc-rich formula goat milk powder and a preparation method thereof. The zinc-rich formula goat milk powder is prepared from the following raw materials in parts by weight: 360-460 parts of raw goat milk, 300-350 parts of D90 desalted whey powder, 80-140 parts of WPC34 concentrated whey protein powder, 5-15 parts of galactooligosaccharide, 0.1-1 part of yeast beta-glucan, 50-120 parts of flax seed oil, 1-3 parts of a nutrient supplement, 1-5 parts of phytosterol ester, 20-60 parts of isomaltose hypgather, 10-50 parts of lactoferrin, 1-5 parts of alpha-lactalbumin and 1-3 parts of soybean lecithin. The zinc-rich formula goat milk powder disclosed by the invention is balanced in nutrient ingredients and can be used for promoting the growth and development of children and weak people.

The present invention provides a sleep quality improving solid drink composition. The composition is prepared from the following raw materials in parts by weight: 0.5-5 parts of alpha-lactalbumin, 0.5-5 parts of a casein hydrolyzate, 0.5-5 parts of gamma-aminobutyric acid, 29-65 parts of dairy products and 26-69.5 parts of other auxiliary materials. The solid drink composition contains high-quality proteins, is good in mouthfeel and convenient for taking, has effects of improving sleep quality in a regular consumption, and is particularly suitable for a consumption by people suffering from sleeping problems.

The invention relates to a method for testing components in milk, especially to a testing method for testing the content of alpha-lactalbumin, which pertains to the analysis technics field. The inventive testing method for testing the content of alpha-lactalbumin using a capillary pipe cataphoresis apparatus, includes sample pretreatment, sample loading testing, reading calibration and the like steps. In the invention, the sample runs electrophoresis on the capillary pipe cataphoresis apparatus, thereby optical suction values of samples tested by a capillary pipe chromatograph system can be expressed in a spectrogram form, and the content of alpha-lactalbumin can be accurately tested, which provides technique supporting for the products development, quality control and component analyzing.

The present invention relates to a pharmaceutical composition comprising monomeric alpha-lactalbumin complex, preferably LAC, which is an active complex of alpha-lactalbumin and a fatty acid or lipid with selective cytotoxic activity. The composition of the invention comprises insignificant amounts of oligomeric / multimeric alpha-lactalbumin complex, preferably LAC. Based on the selective cytotoxicity of the alpha-lactalbumin complex, preferably LAC composition such compositions are suitable for use in the manufacture of medicaments for use in therapy. Medicaments, comprising monomeric LAC are for use in the treatment of bacterial and viral infections and in particular cancer due to the selective cytotoxic activity. The application further relates to methods of producing a composition comprising monomeric alpha-lactalbumin complex, preferably LAC with cytotoxic activity.

The invention relates to enhancing functionalized infant formula milk powder prepared from the raw materials of, by weight, 90-95 parts of cow milk powder, 1-3 parts of alpha-lactalbumin, 2-3 parts of galactooligosaccharide, 1-2 parts of fructooligosaccharide, 0.2-0.4 parts of probiotics, 0.05-0.1 lactoferrin, 0.05-0.1 parts of nucleotide, 0.08-0.15 parts of DHA, 0.16-0.3 parts of ARA, 0.1-0.18 parts of choline chloride, 0.05 parts of composite vitamin, and 0.5 parts of composite mineral. The infant formula milk powder provided by the invention assists in nursing intestines of infants, enhancing infant mental development, and improving immunity.