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Enzymatic hydrolysis alpha-lactalbumin polypeptide and enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle and preparation thereof

A lactalbumin and nano-micelle technology, which is applied in the field of enzymatic hydrolysis of α-lactalbumin polypeptide and nano-micelle of α-lactalbumin polypeptide, which can solve the problems of toxicity, large micelle particle size, and complicated preparation process

Active Publication Date: 2017-05-24
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the carrier prepared by this method has certain toxicity, the prepared micelle particle size is relatively large, and the preparation process is relatively complicated.

Method used

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  • Enzymatic hydrolysis alpha-lactalbumin polypeptide and enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle and preparation thereof
  • Enzymatic hydrolysis alpha-lactalbumin polypeptide and enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle and preparation thereof

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preparation example Construction

[0060] The present invention further relates to a preparation method of loaded enzymatically hydrolyzed α-lactalbumin polypeptide nanomicelles, which comprises:

[0061] Step A, mixing the hydrophobic substance solution with the above-mentioned enzymatic α-lactalbumin polypeptide or the enzymatic α-lactalbumin polypeptide solution prepared by the above method, and stirring evenly to make loaded enzymatic α-lactalbumin polypeptide nanomicelles Base fluid;

[0062] Step B, the loaded enzymatically hydrolyzed α-lactalbumin polypeptide nano-micelle base solution is reacted at room temperature for 30-60 minutes, preferably 30-40 minutes, and more preferably after 30 minutes, to prepare the loaded enzymatically hydrolyzed α-lactalbumin polypeptide nano micelles;

[0063] Wherein, in the loaded enzymatically hydrolyzed α-lactalbumin polypeptide nanomicelle base fluid, the mass ratio of the hydrophobic substance to the enzymolyzed α-lactalbumin polypeptide is (1-3):5.

[0064] In on...

Embodiment 1

[0082] Example 1: Preparation of enzymatically hydrolyzed α-lactalbumin polypeptide.

[0083] 1) Accurately weigh 6 mg of α-lactalbumin into a 1.5 mL centrifuge tube, add 200 μL of pH=7.5, 75 mM Tris-HCl buffer solution, after dissolving, add 1.2 μL of proteolytic enzyme;

[0084] 2) Draw up the solution with a 2mL syringe, and filter the solution once with a 0.2 μm polyethersulfone syringe filter;

[0085] 3) Heat in a water bath at 50°C for 17 minutes, centrifuge and take the supernatant;

[0086] 4) Freeze-drying the supernatant to obtain the enzymatically hydrolyzed polypeptide.

[0087] After detection, the molecular weight of the enzymatically hydrolyzed α-lactalbumin polypeptide is 9.9-11KDa.

Embodiment 2

[0088] Example 2: Preparation of enzymatically hydrolyzed α-lactalbumin polypeptide nanomicelles.

[0089] 1) Accurately weigh 5 mg of the polypeptide and dissolve it in 5 mL of pH=7.5, 75 mM Tris-HCl buffer;

[0090] 2) Rotate and mix for 30 minutes at a speed of 20 rpm to obtain blank polypeptide nanomicelles.

[0091] Observation of these blank polypeptide nanomicelles with a transmission electron microscope, the results are as follows figure 1 shown. from figure 1 It can be seen that the enzymatic hydrolyzed α-lactalbumin polypeptide nano-micelle of the present invention has a small particle size of about 20nm and good dispersibility.

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Abstract

The invention relates to an enzymatic hydrolysis alpha-lactalbumin polypeptide and an enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle and preparation thereof. Alpha-lactalbumin with a certain purity as a raw material is used for enzymatic hydrolysis in the presence of a particular enzyme to obtain the ideal enzymatic hydrolysis alpha-lactalbumin polypeptide, the enzymatic hydrolysis alpha-lactalbumin polypeptide can be used for preparing the stable particle size enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle, the raw material is low in cost and easy to obtain, and the preparation process is simple; and the micelle is small in particle size, good in dispersion, good in biocompatibility and high in stability, the micelle can be used for embedding of hydrophobic materials, the micelle as a carrier can be made into a supported enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle, and the supported enzymatic hydrolysis alpha-lactalbumin polypeptide nano micelle has the advantages of small particle size, good stability, high encapsulation efficiency and non-toxicity.

Description

technical field [0001] The invention belongs to the technical field of nano-micelle preparation, and relates to enzymatic hydrolysis of alpha-lactalbumin polypeptide, enzymatic hydrolysis of alpha-lactalbumin polypeptide nano-micelle and its preparation and application. Background technique [0002] Polymer micelle is a new type of nano-drug carrier that has emerged in recent years. It is an amphiphilic block copolymer that is dissolved in water and spontaneously forms a hydrophobic inner core and A self-assembled structure of a hydrophilic shell. It has the following advantages: ①The hydrophobic inner core can solubilize poorly water-soluble drugs, have a high drug loading capacity, and realize controllable release of drugs. ② The particle size is about 10-200nm, the particle size is small and uniform, and the shell is hydrophilic and flexible, which can greatly reduce the chance of micelles being recognized and taken up by the reticuloendothelial system (RES), and the cir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K9/107A61K47/42
Inventor 李媛李唯
Owner BEIJING UNIV OF CHEM TECH
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