Method for detecting a-lactalbumin content in cow's milk

A technology of lactalbumin and detection method, which is applied in the field of detection of a-lactalbumin content in milk, and can solve problems such as complicated test steps

Inactive Publication Date: 2008-10-08
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

The test steps of this method are complica...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 A kind of detection method of α-lactalbumin content in milk

[0026] The capillary electrophoresis temperature was controlled at 30°C, a quartz capillary column with a diameter of 50 μm and a length of 600 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 5 s, and the working voltage was 25 kV.

[0027] Establishment of calibration curve: standard solutions with concentrations of 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, 0.1 mg / ml and 0.05 mg / ml were prepared using a-lactalbumin standard. After shaking well, use high-pressure capillary electrophoresis analysis. The integrated seal areas are 282154, 140793, 66924, 16810, and 3520 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.9993, the lowest detection line is 0.08ug, the lowest detection line The output content is 0.02mg / ml, and the standard sample recovery rate reaches 94%.

[0028] Processing sample b...

Embodiment 2

[0032] Embodiment 2 The detection method of α-lactalbumin content in a kind of milk

[0033] The capillary electrophoresis temperature was controlled at 40°C, a quartz capillary column with a diameter of 75 μm and a length of 50 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 10 s, and the working voltage was 25 kV.

[0034]Establishment of calibration curve: standard solutions with concentrations of 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, 0.1 mg / ml and 0.05 mg / ml were prepared using a-lactalbumin standard. After shaking well, use high-pressure capillary electrophoresis analysis. The integrated seal areas are 292457, 151022, 71042, 18245, and 3700 respectively. The standard curve is obtained by using the software. The linear regression correlation coefficient is 0.9982, the lowest detection line is 0.09ug, the lowest detection line The output content is 0.025mg / ml, and the standard sample recovery rate reaches 93%.

[0035] Processing ...

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Abstract

The invention relates to a method for testing components in milk, especially to a testing method for testing the content of alpha-lactalbumin, which pertains to the analysis technics field. The inventive testing method for testing the content of alpha-lactalbumin using a capillary pipe cataphoresis apparatus, includes sample pretreatment, sample loading testing, reading calibration and the like steps. In the invention, the sample runs electrophoresis on the capillary pipe cataphoresis apparatus, thereby optical suction values of samples tested by a capillary pipe chromatograph system can be expressed in a spectrogram form, and the content of alpha-lactalbumin can be accurately tested, which provides technique supporting for the products development, quality control and component analyzing.

Description

technical field [0001] The invention relates to a method for analyzing and detecting protein in milk, in particular to a method for detecting a-lactalbumin content in milk. It belongs to the field of analysis technology. Background technique [0002] At present, there is no national standard or industry standard for the detection method of α-lactalbumin content in China. Domestic related standards include GB / T 5413.2-1997 Determination of Infant Formula Food and Milk Powder-Whey Protein. Abroad, relevant national standards include DIN-10466-2001 Determination of whey protein content in total protein of milk and milk products, DIN-10470-1999 Determination of milk protein content and casein content in total protein of milk and milk products, DIN-10472 -1996 Determination of milk protein and casein components in the total protein content of milk and dairy products. Electrophoresis method. The commonly used detection method for α-lactalbumin is liquid chromatography. The tes...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/447G01N33/04
Inventor 孙国庆康小红胡新宇刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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