31results about How to "Effective detection content" patented technology

The invention discloses a fritillaria medicinal material identification and content determination method comprising the steps: after extracting with ethanol, passing through a neutral alumina column, determining by high performance liquid chromatography, combining with specific chromatograms of medicinal materials, effectively identifying thunberg fritillary bulb, bulbus fritillariae cirrhosae and bulbus fritillariae ussuriensis, and determining the contents of peimine and peiminine. The method not only can effectively identify authenticity of the three fritillaria medicinal materials, but also can accurately determine for the contents of peimine and peiminine, achieves multiple evaluations through one-time determination, and is safe, environmentally friendly, convenient and quick.

The invention discloses a method for determining content and purity of L-carnitine in milk powder, comprising the following steps in sequence: 1) taking the milk powder as a sample for sample treatment; 2) preparing standard solutions respectively by taking an L-carnitine standard substance and a D-carnitine standard substance; 3) correspondingly preparing an L-carnitine standard solution and a D-carnitine standard solution obtained the step 2) into an L-carnitine derivative solution containing gradient concentration and a D-carnitine solution containing gradient concentration; 4) carrying out derivatization reaction on the sample solutions; 5) carrying out high performance liquid chromatography on the products obtained in the step 3) and step 4); 6) acquiring the concentration cL of the L-carnitine and the concentration cD of the D-carnitine; and 7) acquiring the content and the purity of the L-carnitine in the milk powder sample. By adopting the method for determining the content and the purity of L-carnitine in the milk powder of the invention, the detection sensitivity can be greatly improved.

The invention provides a method for testing components in milk, especially a method for testing peroxidase content in milk. The invention belongs to the field of testing technology. The method for testing peroxidase content in milk by a capillary electrophoresis apparatus provided by the invention includes the steps of pretreatment, sample test, and also includes the steps of calibrating readings, editing reports and so on. The invention includes the following steps: doing electrophoresis by the capillary electrophoresis apparatus, detecting the absorption value of the sample on the capillary chromatograph system, expressing the value in map form, then the peroxidase content in milk is detected accurately, in order to provide technical supports for product development, quality control and component analysis.

The invention relates to an extracting method and a detecting method for hemp compounds in hair. The extracting method includes: (1) cleaning the hair, and grinding the hair with liquid nitrogen to obtain hair powder; (2) adding the ground hair powder into a sodium hydroxide solution, ultrasonic treating, centrifuging to obtain supernatant, and adding phosphate buffer to buffer so as to obtain treated hair liquid; (3) pouring the treated hair liquid into a balanced solid-phase extraction column, successively washing off impurities with an aqueous acetic acid solution and an aqueous methyl alcohol solution, eluting with a n-hexane-acetic acid solution, and collecting eluant to obtain the hemp compounds. The collected eluant is detected by an LC-MS / MS (liquid chromatogram-mass spectrum / massspectrum) method, rare tetrahydrocannabinol and tetrahydrocannabinol metabolites content in the hair can be detected effectively. The extracting method is simple to operate, mild in extracting conditions and high in extracting speed and releasing efficiency, and the detecting method is simple to operate and high in detection sensitivity.

The invention discloses a method for detecting 2,2,6,6-tetramethylpiperidinooxy with high performance liquid chromatography-mass spectrometry. The method comprises the steps that 1, a test solution and a reference stock solution is prepared; 2, the test solution and the reference stock solution with a certain concentration gradient are subjected to sample introduction, a high performance liquid chromatograph mass spectrometer is used for detecting and recording a chromatogram; and 3, linear regression analysis is performed on the mass concentration of the reference stock solution and chromatogram peak area, a regression equation and correlation coefficients are obtained, and a standard curve is manufactured; and the peak area of 2,2,6,6-tetramethylpiperidinooxy in the chromatogram of the test solution is utilized to calculate the content of 2,2,6,6-tetramethylpiperidinooxy by an external standard method. The method provided by the invention is the first method for detecting 2,2,6,6-tetramethylpiperidinooxy developed in the field. The method has accurate detection result, high sensitivity, good stability, and low detection limit, and totally meets detection requirements for genotoxic impurities in the field.

The invention relates to a method for detecting components in milk, in particular to a method for detecting the content of Beta-lactoglobulin, belonging to the detection technical field; the detection method using a capillary electrophoresis meter to detect the content of Beta-lactoglobulin of the invention comprises the following steps: the pretreatment of samples, detection of sample-loading, alignment of reading and edit of report, etc.; the invention is that the samples are processed by electrophoresis on the capillary electrophoresis meter, thus detecting absorbance of the samples by a capillary chromatographic system, expressing the absorbance by the mode of mapping, detecting the content of Beta-lactoglobulin accurately and providing the technical support for the development of products, quality control and analysis of the components.

The invention relates to a method for detecting components in milk, in particular to a detection method for detecting the content of milk immunoglobulin (IgG), and belongs to the detection technology field. The detection method for detecting the content of the milk immunoglobulin (IgG) through a capillary electrophoresis instrument of the invention comprises the steps of sample pretreatment and sample loading detection, reading calibration, and report editing, etc. In the invention, the electrophoresis of a sample runs on the capillary electrophoresis instrument, and the absorption value of the sample is detected by a capillary chromatographic system and expressed in the form of mapping, thereby the content of the milk immunoglobulin (IgG) can be accurately detected, so as to provide technology support for product development, quality control and component analysis.

The invention discloses a stable and sensitive type IV collagen (IV-C) detection reagent. The stable and sensitive type IV collagen (IV-C) detection reagent comprises a reagent R1 and a reagent R2; areaction system is optimized, TAPSO (N-[Tris(hydroxymethyl)metyl]-3-amino-2-hydroxypropanesulfonic acid) buffer is adopted, a plurality of stabilizing agents including polyvinylpyrrolidone, gamma-cyclodextrin, xanthan gum, NaCl, trehalose, PEG 6000, and glycerol are added, so that reagent stability is improved obviously; goat anti-human type IV collagen antibody coated latex particles are adopted,in detection, mutual crosslinking of adjacent latex particles is realized through antigen-antibody specific immunization reaction in detection, so that reagent sensitivity and accuracy are increasedobviously; and in addition, combination of novel nonionic surfactants RHEODOL, TW-O320V (tween 85) and AMINON C-11S (methyl cocoate alcohol amide) is adopted, so that antibody stability is promoted and maintained, system turbidity is avoided, and reagent stability is improved further.

The invention relates to a method for detecting the compositions of the milk, in particular to a method for detecting the content of lactoferrin, belonging to the detection technical field. The method for detecting the content of lactoferrin by a capillary electrophoresis apparatus comprises the following steps of pretreatment of a sample, the sample detection, the calibration and reading, and the edition and report, etc. The electrophoresis is passed through the sample by the capillary electrophoresis apparatus, thereby detecting the absorbance value of the sample by the capillary electrophoresis apparatus, displaying the absorbance value in a form of map, detecting the content of lactoferrin accurately and providing the technical support for the product research, the quality control and the component analysis.

The invention relates to the technical field of organosilicon polymer detection, and concretely relates to a method for measuring the content of aldehyde ketone in polyether modified organosilicon. The method comprises the following steps: carrying out a derivation reaction on to-be-detected polyether modified organosilicon and DNPH to form a reaction product; taking polyether modified organosilicon. not containing the to-be-detected polyether as a blank control, and carrying out the derivation reaction to form a blank control product; respectively detecting the reaction product and the blank control product by utilizing high performance liquid chromatography to respectively obtain peak areas of different aldehyde ketones, respectively substituting the peak areas of different aldehyde ketones into a standard curve formula of the corresponding aldehyde ketones, and calculating to respectively obtain the detection contents of the corresponding aldehyde ketones; and substituting the detected content of the corresponding aldehyde ketone into a formula to obtain the content of the aldehyde ketone corresponding to the polyether modified organosilicon to be detected, wherein the formula is as follows: W = (Ai - A0) / m. The method can effectively, accurately and quantitatively detect the content of aldehyde ketone in the polyether modified organic silicon, and is beneficial to improving the quality of the polyether modified organosilicon.

The invention relates to an ultra-high performance liquid chromatography analysis method for detecting mannose in honey. The method comprises the following steps: performing linear equation and standard chromatogram, performing sample pretreatment, and performing ultra-high performance liquid chromatography condition detection on a pretreated sample. The method can effectively detect the content of mannose in the honey, has good sensitivity, and can identify whether the honey is adulterated according to the content of mannose in the honey.

The invention provides a device for noninvasively detecting indocyanine green content in bodies. The device includes a laser emitting device that can emit single wavelength or multi-wavelength with the wavelength of 805 nm, and photoacoustic signal detection and conversion devices; and preferably, the device also includes an ultrasonic positioning device, and the ultrasonic positioning device canaccurately detect the indocyanine green content in a blood vessel at a specific position without being disturbed by other tissue. Through the device, the noninvasive detection on the indocyanine greencontent in blood can be realized; rapid assessment on hepatic functional reserve can be realized; and therefore, the device has good commercial prospects.

The invention belongs to the field of drug detection, and particularly relates to a method for determining photodegradation impurities in a levofloxacin raw material and a levofloxacin preparation. The method comprises the following steps: (1) preparing a levofloxacin photodegradation impurity standard solution; (2) injecting the standard solution into a high performance liquid chromatograph for determination, and constructing a standard curve by taking the concentration of the standard solution as an abscissa and the peak area as an ordinate; (3) preparing a to-be-detected sample solution; and (4) injecting the to-be-detected sample solution into a high performance liquid chromatograph for determination, recording the peak area of the to-be-detected sample solution, and calculating the content of the photodegradable impurities by using the standard curve. According to the method, high performance liquid chromatography is adopted, amino silane bonded silica gel is used as a stationaryphase, a mixed solution of acetonitrile and 0.05mol / L monopotassium phosphate solution is used as a mobile phase, and an isocratic elution mode is adopted. The method is high in specificity, high in precision, good in accuracy and capable of effectively detecting the content of photodegradation impurities in levofloxacin raw materials and preparations.

The invention discloses a method for detecting an afoxolaner intermediate by reversed-phase high performance liquid chromatography. The arfanaga intermediate is 4-oximidomethyl-1-naphthoic acid. The method comprises the following steps: 1) preparing a test solution; 2) detecting the test solution by using reversed-phase high performance liquid chromatography; and 3) determining the content of the afoxolaner intermediate in the test solution by using an area normalization method. The complete separation of the chromatographic peaks in the test solution can be achieved through a simple mobile phase component, the detection result precision is high, the repeatability is good and stable, and the purity of the afoxolaner intermediate can be accurately controlled.

The invention discloses an enzyme-linked immune sorbent assay (ELISA) kit for detecting Shiga toxin II (StxII) and also discloses sequence information of monoclonal antibodies S2D8 and S2C6 in the ELISA kit and a preparation process of the monoclonal antibodies S2D8 and S2C6. The ELISA kit comprises the monoclonal antibody S2D8, the HRP-marked monoclonal antibody S2C6, a horse radish peroxidase substrate buffer solution, a protein standard product StxII (100 ug / ml, 0.1 ml), a negative control sample BSA and lotion (PBS+0.05%Tween20). The kit can sensitively detect the StxII content of samples, and the sensitivity can reach 4 ng / ml. The kit provided by the invention also can effectively identify subtype StxIIc and StxIIvha of the StxII.

Preparation of CNTs / S‑Cu for electrochemical detection of glucose by monovalent copper hydrolysis 2 O, the present invention relates to a kind of CNTs / S-Cu that utilizes monovalent copper hydrolysis to prepare for the electrochemical detection of glucose 2 O Composite Methods. The invention aims to solve the problems of poor electrical conductivity and low catalytic activity when cuprous oxide is used for glucose electrochemical detection. Preparation of CNTs / S‑Cu for electrochemical detection of glucose by monovalent copper hydrolysis 2 O: (1) Preparation of acidic monovalent copper solution; (2) Preparation of CNTs aqueous solution; (3) Preparation of CNTs-Cu(Ⅰ) aqueous solution; (4) Preparation of CNTs / S-Cu by hydrolysis 2 O. CNTs / S‑Cu prepared by monovalent copper hydrolysis 2 The O composite material has excellent electrical conductivity and large specific surface area, so it shows a good linear relationship and linear range when used in the electrochemical detection of glucose.

The invention discloses a detection method of detecting trimethylsulfonium in tea leaves by a liquid chromatograph-tandem mass spectrometer. The detection method comprises the following steps of 1) sample pretreatment; and 2) chromatogram and mass spectrum conditions of detection with liquid chromatography-tandem mass spectrometry: a mobile phase: organic phase is acetonitrile, a water phase is anammonium acetate-formic acid solution which has the concentration of 15mmol / L and contains 1.5mL of formic acid per L; gradient elution: a flow rate is 0.30mL / min, and a feeding amount is 25 microlitres; ion source: electrospray ionization (ESI) with positive ion scanning; a mass spectrum scanning manner is multiple reaction monitoring (MRM); spray voltage is 4,200V; shell gas pressure is 40arb;and collision gas (Ar / m) Torr: 1.5. By adopting the detection method, the recovery rate and the precision accord with relevant technical specifications, residues of the trimethylsulfonium in the tea leaves can be effectively detected, and the detection requirements for a residue limit of the trimethylsulfonium in the tea leaves of the European Union are met.

The invention discloses an enzyme-linked immune sorbent assay (ELISA) kit for detecting Shiga toxin II (StxII) and also discloses sequence information of monoclonal antibodies S2D8 and S2C6 in the ELISA kit and a preparation process of the monoclonal antibodies S2D8 and S2C6. The ELISA kit comprises the monoclonal antibody S2D8, the HRP-marked monoclonal antibody S2C6, a horse radish peroxidase substrate buffer solution, a protein standard product StxII (100 ug / ml, 0.1 ml), a negative control sample BSA and lotion (PBS+0.05%Tween20). The kit can sensitively detect the StxII content of samples, and the sensitivity can reach 4 ng / ml. The kit provided by the invention also can effectively identify subtype StxIIc and StxIIvha of the StxII.

The invention relates to a method for detecting human milk ingredient, in particular to a method for detecting immune globulin IgM content in human milk, comprising: (1) dissolving a human immune globulin IgM standard substance into sample buffer solution to prepare to obtain multiple standard solutions with different contents of the standard substance, using a high voltage capillary electrophoresis analyser to respectively detect and analyze the standard solution to obtain a detection result, and drawing a standard curve according to the concentration of the standard solution and the corresponding detection result; (2) after a human milk sample is centrifuged, utilizing the high voltage capillary electrophoresis analyser to detect the sample; (3) comparing the detection result of the sample with the standard curve to obtain the human immune globulin IgM content in human milk sample. The invention has the characteristics of accurate detection result, high repeatability and low cost.

The invention relates to a method for detecting the immunoglobulin IgA content in human milk, in particular to a detection method for detecting immunoglobulin IgA content in human milk. The method comprises the following steps: (1) dissolving human immunoglobulin IgA standards in sample buffer solutions, preparing multiple standard solutions with different standard contents, using a high-pressure capillary electrophoresis apparatus to respectively detect and analyze the standard solutions to obtain detection results, and drawing standard curves based on the concentrations of the standard solutions and the corresponding detection results; (2) centrifuging a human milk sample, and then detecting the sample by using the high-pressure capillary electrophoresis apparatus; and (3) comparing the detection result of the sample with the standard curves to obtain the human immunoglobulin IgA content in the human milk sample. The invention has the characteristics of accurate detection result, high repeatability and low cost.

The invention relates to a method which uses capillary electrophoresis to detect the content of beta-casein protein in milk; and the method includes the following steps: mixing a milk sample to be detected and sample buffer solution; and detecting the sample through the capillary electrophoresis after hydrid processing. The preparation steps of the sample buffer solution is as follows: adding 3-morpholine substituting propane sulfonic acid, disodium EDTA and urea into the trimethyl tris buffer solution; and adding beta-mercaptoethanol and methyl hydroxyethylcellulose when the sample is processed. The steps of processing the milk sample comprise centrifugally processing of the milk sample, mixing the supernatant of the sample after being processed centrifugally and the sample buffer solution, and cleaning the mixture with ultrasonic waves. The method has the advantages of excellent effects of separation and high speed; the capillary column without coating can be applied in the method. The correlation coefficient of linear regression equation of the method is more than 0.98; the minimum detectable concentration is 0.005mg / ml; and the accuracy is above 90 percent.

The invention discloses a method for measuring free water content and bound water content of animal tissues. The method comprises the following steps: preparing animals, tumor strains and instruments;dividing the animals into a physiological group and a pathological group; injecting H22 cells into the armpits of the animals in the pathological group and establishing an H22 tumor-bearing model; molding the H22 tumor-bearing model for 14 days and taking out the right back limb tissue; heating; performing data processing; performing statistical analysis. The method has the following advantages:the free water content and the bound water content of the animal tissues can be effectively detected, different tissues need to be baked for different time to achieve constant weight, through heatingand weighing, the free water in the physiological group must be heated at 75 DEG C for 4 hours and the free water in the pathological group must be heated at 75 DEG C for 5 hours; weak bound water ofthe physiological group and the pathological group must be heated at 100 DEG C for 2 hours, strong bound water of the physiological group and the pathological group must be heated at 120 DEG C for 1 hour, and research on the free water and bound water in the animal tissues is beneficial to early diagnosis of tumor diseases and research on anti-tumor medicines and can be applied to research on anti-ageing medicines.

The invention provides a method for testing components in milk, especially a method for testing peroxidase content in milk. The invention belongs to the field of testing technology. The method for testing peroxidase content in milk by a capillary electrophoresis apparatus provided by the invention includes the steps of pretreatment, sample test, and also includes the steps of calibrating readings, editing reports and so on. The invention includes the following steps: doing electrophoresis by the capillary electrophoresis apparatus, detecting the absorption value of the sample on the capillarychromatograph system, expressing the value in map form, then the peroxidase content in milk is detected accurately, in order to provide technical supports for product development, quality control andcomponent analysis.