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Extracting method and detecting method for hemp compounds in hair

A technology of cannabinoid compounds and extraction methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not meeting the detection requirements, low THC content, increasing sample collection volume, etc., and achieves good accuracy and extraction Fast, easy-to-use effects

Inactive Publication Date: 2018-11-13
广州正孚检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following disadvantages: 1) extraction with methanol takes a long time, low extraction efficiency, and poor timeliness; 2) THC content in hair is low, and the lower limit of quantification of 1ng / mg cannot meet the requirements of actual drug-related cases. Testing requirements; 3) The content of THC in hair is lower than that of THC, and the linear range of 1ng / mg to 20ng / mg of THC in hair cannot match the actual drug-related cases; 4) The amount of hair sampled in a single experiment is relatively large, which increases the amount of sample collected

Method used

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  • Extracting method and detecting method for hemp compounds in hair
  • Extracting method and detecting method for hemp compounds in hair
  • Extracting method and detecting method for hemp compounds in hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This example is two identification cases of drug-related cases accepted by our laboratory in 2016.

[0043] 1. Experimental steps:

[0044] (1) Hair collection: Cut the hair with a length of not less than 1cm by sticking to the skin, place it on aluminum foil paper, wrap and fold it, and store it in a clean ziplock bag at room temperature and away from light.

[0045] (2) Weigh about 50 mg of hair each time, wash with 3 mL of detergent, 3 mL of water, and 3 mL of acetone in sequence, cut into 1.0 mm fragments, and grind with liquid nitrogen to obtain hair powder.

[0046] (3) Weigh 20 mg of hair powder, add 3 mL of 1mol / L sodium hydroxide solution, add internal standard substances (D3-11-OH-THC and D9-THC-COOH), and ultrasound at 70°C for 15 minutes , Centrifuge, take the supernatant and add 1 mL of phosphate buffer (pH 6.0) for buffering to obtain the hair pretreatment solution.

[0047] (4) After equilibrating the solid phase extraction column with 0.5mL methanol and 0.1mL ace...

Embodiment 2

[0077] In this example, the negative hair was added with tetrahydrocannabinol and tetrahydrocannabinolic acid as the standard substance, and the LC-MS / MS quantitative analysis was performed after pretreatment with sodium hydroxide solution and solid phase extraction. Prepare standard supplementary samples with concentrations of 50pg / mg and 12000pg / mg respectively. Prepare 6 samples for each concentration on the same day, measure the ratio of each component to the peak area of ​​the internal standard, calculate the concentration and CV value, determine the recovery rate of the method and the intraday detection precision; prepare the two concentrations separately for 5 consecutive days Standard add samples, measure the ratio of each component to the peak area of ​​the internal standard, calculate the CV value, and determine the precision and accuracy of the method for daytime detection. Please see Table 3 and Table 4 for the experimental results.

[0078] 1. Experimental steps:

[...

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Abstract

The invention relates to an extracting method and a detecting method for hemp compounds in hair. The extracting method includes: (1) cleaning the hair, and grinding the hair with liquid nitrogen to obtain hair powder; (2) adding the ground hair powder into a sodium hydroxide solution, ultrasonic treating, centrifuging to obtain supernatant, and adding phosphate buffer to buffer so as to obtain treated hair liquid; (3) pouring the treated hair liquid into a balanced solid-phase extraction column, successively washing off impurities with an aqueous acetic acid solution and an aqueous methyl alcohol solution, eluting with a n-hexane-acetic acid solution, and collecting eluant to obtain the hemp compounds. The collected eluant is detected by an LC-MS / MS (liquid chromatogram-mass spectrum / massspectrum) method, rare tetrahydrocannabinol and tetrahydrocannabinol metabolites content in the hair can be detected effectively. The extracting method is simple to operate, mild in extracting conditions and high in extracting speed and releasing efficiency, and the detecting method is simple to operate and high in detection sensitivity.

Description

Technical field [0001] The invention relates to biomedicine detection technology, in particular to a method for extracting and detecting cannabis compounds in hair. Background technique [0002] In recent years, the hair samples have more comprehensive information about drug abuse because of their responses. In order to confirm whether the examinee is taking drugs and the degree of drug ingestion, such samples are often used as evidence for judicial identification and forensic science, and are also widely used in clinical toxicology. , Doping control and other fields. [0003] The composition of hair is complex, and there are a large number of interfering substances of organic components. In order to detect the THC in the hair, a specific pretreatment method must be established to separate and extract the target substance. Because tetrahydrocannabinol and tetrahydrocannabinol acid are acidic compounds, they are more difficult to enter into black hair than other alkaloid drugs, so ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 刘晓云王平何小维王继华
Owner 广州正孚检测技术有限公司
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