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A Screening Method for Shrimp Health Indicator Bacteria

A health status and screening method technology, applied in data processing applications, instruments, sequence analysis, etc., can solve the problems of high professional technical requirements, high cost, routine assessment and early warning of difficult shrimp diseases, etc., to reduce risks and improve accuracy , the effect of improving economic efficiency

Active Publication Date: 2021-03-23
NINGBO UNIV
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Problems solved by technology

However, high-throughput sequencing technology has the characteristics of long cycle, high cost and high professional technical requirements, so it is difficult to apply to the routine assessment and early warning of shrimp diseases in the breeding process

Method used

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  • A Screening Method for Shrimp Health Indicator Bacteria
  • A Screening Method for Shrimp Health Indicator Bacteria
  • A Screening Method for Shrimp Health Indicator Bacteria

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Embodiment Construction

[0028] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0029] Specific implementation cases

[0030] From April to July, 2016, starting from April 8, 2016, seedlings of Litopenaeus vannamei were released on 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, Samples were collected from 6 culture ponds after 87 and 93 days. Eighty-four days after stocking, prawns developed disease in 3 of the 6 ponds monitored, and the severity of the disease increased over time, and the mortality rate increased linearly. We collected healthy and diseased shrimp samples at the same time, a total of 103 samples. The samples were extracted from DNA, PCR amplified bacterial 16S rRNA gene, Illumina paired-end sequencing obtained the intestinal bacterial community composition of each sample, and converted into relative abundance; the blood of prawns was drawn to measure the concentration of superoxide dismutase (SOD), comb...

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Abstract

The invention discloses a quantitative prawn disease prediction method based on intestinal tract bacterial indicators. The quantitative prawn disease prediction method is characterized in that prawn health condition indicator bacteriophyta are screened based on bioinformatics, and characteristic design specific primers are formed based on prawn intestinal flora; real-time quantitative PCR is adopted to detect the relative abundance of the health indicator bacteriophyta, the relative abundance of the health indicator bacteriophyta and its indicating weight are used as independent variables to self-establish a prediction model, and quantitative disease prediction during prawn breeding is achieved. The method has the advantages that the influences of diseases on intestinal flora at different growth stages are distinguished, the accuracy rate of prediction is improved, meanwhile the cheap disease prediction method is established and has a very high actual application value, a basis can be provided for formulation of a coping and intervention measure, for example, design of probiotics, and the method has the good pushing effect on reduction of the prawn breeding risk.

Description

technical field [0001] The invention relates to a method for predicting aquaculture diseases, in particular to a method for quantitatively predicting prawn diseases based on intestinal indicator bacteria. Background technique [0002] The prawn farming mode has changed from earth pond culture to intensive, high-density greenhouse and recirculating aquaculture. This transformation has resulted in increased stressors, decreased immunity of prawns, and frequent occurrence of diseases in prawn farming. Shrimp diseases have the characteristics of rapid onset and high mortality, but currently there is no particularly effective treatment method. Under the background that the incidence of prawn diseases has not improved significantly and some areas have aggravated trends, the Ministry of Agriculture formulated a special plan for the prevention and control of major prawn diseases in 2016. The core link is to use drugs safely and rationally under the guidance of disease diagnosis and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B5/00G16B25/20G16B30/10G06Q50/02
CPCG06Q50/02G16B5/00G16B30/00
Inventor 熊金波郁维娜戴文芳
Owner NINGBO UNIV
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