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Method for detecting immunoglobulinlg content in cow's milk

A technology of immunoglobulin and detection method, which is applied in the field of detection of immunoglobulin content in milk, and can solve problems such as undeveloped detection of milk immunoglobulin

Inactive Publication Date: 2008-12-03
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, capillary electrophoresis has not yet been developed to detect milk immunoglobulin (IgG)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 A kind of detection method of immunoglobulin (IgG) content in milk

[0028] The capillary electrophoresis temperature was controlled at 25°C, a quartz capillary column with a diameter of 50 μm and a length of 20 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 10 s, and the working voltage was 15 kV.

[0029] Formulation of the calibration curve: Standard solutions with concentrations of 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, and 0.1 mg / ml were prepared using milk immunoglobulin (IgG) standard products. After shaking well, use high-pressure capillary electrophoresis to analyze. The peak areas after integration are 189614, 87015, 44216, 20897, and 13210 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.9973, the lowest detection line is 0.009ug, the lowest detection line The output content is 0.01mg / ml, and the standard sample recovery rate reac...

Embodiment 2

[0034] Embodiment 2 The detection method of milk immunoglobulin (IgG) content in a kind of bovine milk

[0035] The capillary electrophoresis temperature was controlled at 30°C, a quartz capillary column with a diameter of 75 μm and a length of 600 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 5 s, and the working voltage was 25 kV.

[0036] Formulation of the calibration curve: Standard solutions with concentrations of 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, and 0.1 mg / ml were prepared using milk immunoglobulin (IgG) standard products. After shaking well, use high-pressure capillary electrophoresis to analyze. The peak areas after integration are respectively, 153413, 76214, 37502, 18305, 8923. Use the software to draw the standard curve, the linear regression correlation coefficient is 0.999, the lowest detection line is 0.01ug, the lowest detection line The output content is 0.01mg / ml, and the standard sample recovery rate...

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PUM

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Abstract

The invention relates to a method for detecting components in milk, in particular to a detection method for detecting the content of milk immunoglobulin (IgG), and belongs to the detection technology field. The detection method for detecting the content of the milk immunoglobulin (IgG) through a capillary electrophoresis instrument of the invention comprises the steps of sample pretreatment and sample loading detection, reading calibration, and report editing, etc. In the invention, the electrophoresis of a sample runs on the capillary electrophoresis instrument, and the absorption value of the sample is detected by a capillary chromatographic system and expressed in the form of mapping, thereby the content of the milk immunoglobulin (IgG) can be accurately detected, so as to provide technology support for product development, quality control and component analysis.

Description

technical field [0001] The invention relates to a method for analyzing and detecting protein in milk, in particular to a method for detecting immunoglobulin (IgG) content in milk. It belongs to the technical field of detection. Background technique [0002] Milk is an indispensable nutrient in daily life. It is rich in nutrients, such as casein, whey protein, complement, fat and immunoglobulin. The immunoglobulins contained in milk are mainly IgG, IgA and IgM. Among them, IgG content is the highest, about 80% of the total immunoglobulin. The content of immunoglobulin in the endocrine colostrum of cows on the first day after delivery is the highest. Studies have shown that the average concentration of IgG can reach 59-80mg / ml. However, the IgG concentration in the colostrum on the 5th to 7th day is close to the normal milk level, about 0.7-1.0 mg / ml. Immunoglobulin has a variety of biologically active functions, mainly to identify and resist invading bacteria and viruses ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N30/00
Inventor 孙国庆康小红胡新宇刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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