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Cow milk lactoferrin content checking method

A technology for lactoferrin and detection method, applied in the field of analysis and detection of protein in milk, can solve problems such as undeveloped detection of lactoferrin

Inactive Publication Date: 2008-10-22
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, capillary electrophoresis has not yet been developed to detect lactoferrin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 A kind of detection method of lactoferrin content in cow's milk

[0028] The capillary electrophoresis temperature was controlled at 25°C, a quartz capillary column with a diameter of 50 μm and a length of 20 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 13 s, and the working voltage was 15 kV.

[0029] Formulation of the calibration curve: Standard solutions with concentrations of 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, 0.1 mg / ml and 0.05 mg / ml were prepared using lactoferrin standard products. After shaking well, use high-pressure capillary electrophoresis analysis. The peak areas after integration are 120408, 62151, 33070, 16172, and 7829 respectively. The standard curve is obtained by using software. The linear regression correlation coefficient is 0.9993, the lowest detection line is 0.005ug, the lowest detection line The output content is 0.01mg / ml, and the standard sample recovery rate reaches 90%.

[0030]...

Embodiment 2

[0034] Embodiment 2 A kind of detection method of lactoferrin content in cow's milk

[0035] The capillary electrophoresis temperature was controlled at 30°C, a quartz capillary column with a diameter of 75 μm and a length of 600 mm was used, a DAD detector was used, the injection pressure was 0.5 psi, the injection time was 5 s, and the working voltage was 25 kV.

[0036] Formulation of the calibration curve: Standard solutions with concentrations of 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, 0.1 mg / ml and 0.05 mg / ml were prepared using lactoferrin standard products. After shaking well, use high-pressure capillary electrophoresis to analyze. The peak areas after integration are respectively, 157203, 78413, 38450, 18625, 9512. Use the software to draw the standard curve, the linear regression correlation coefficient is 0.998, the lowest detection line is 0.01ug, the lowest detection line The output content is 0.01mg / ml, and the standard sample recovery rate reaches 95%.

[0037] Process...

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PUM

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Abstract

The invention relates to a method for detecting the compositions of the milk, in particular to a method for detecting the content of lactoferrin, belonging to the detection technical field. The method for detecting the content of lactoferrin by a capillary electrophoresis apparatus comprises the following steps of pretreatment of a sample, the sample detection, the calibration and reading, and the edition and report, etc. The electrophoresis is passed through the sample by the capillary electrophoresis apparatus, thereby detecting the absorbance value of the sample by the capillary electrophoresis apparatus, displaying the absorbance value in a form of map, detecting the content of lactoferrin accurately and providing the technical support for the product research, the quality control and the component analysis.

Description

technical field [0001] The invention relates to a method for analyzing and detecting protein in milk, in particular to a method for detecting lactoferrin content in milk. It belongs to the technical field of detection. Background technique [0002] Lactoferrin is a glycoprotein with a molecular weight of 70-80kD, which is widely present in milk, saliva, tears and other exocrine fluids, plasma, and neutrophils. The content of lactoferrin in milk is 0.7g / L. Lactoferrin is a multifunctional protein with broad-spectrum antibacterial and antiviral infection effects, and can regulate the balance of iron in the body; regulate the production of bone marrow cells and promote cell growth; regulate the body's immune function and enhance the body's disease resistance; inhibit The role of human tumor cells; it can work synergistically with a variety of antibiotics and antifungal agents to treat diseases more effectively. [0003] At present, there is no national standard or industry s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N30/00
Inventor 孙国庆康小红胡新宇刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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