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Determination method of content of fulvic acid in fermentation liquor

A method of determination and technology of fermented liquid, applied in the direction of chemical analysis by titration method, etc., can solve problems such as difficult absorption and utilization, inappropriateness, and easy precipitation

Active Publication Date: 2014-01-29
SAAS BIOTECH & NUCLEAR TECH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Brown black humic acid is a high-molecular organic acid, which is difficult to be absorbed and utilized by plants. Especially as a foliar fertilizer or liquid fertilizer, it is easy to flocculate and precipitate under the action of divalent ions such as Cu, Fe, Zn, and Mn. Therefore, , Generally, flocculation occurs during the production process, and it is difficult to become a true solution. Even if a chelating agent is used, it is easy to be precipitated in hard water
Spraying on the leaves of crops is also difficult to be absorbed by crops
It can be seen that in the "humic acid-containing water-soluble fertilizer", it is inappropriate to use the acid precipitation redox titration method to exclude low-molecular-weight fulvic acid substances.

Method used

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  • Determination method of content of fulvic acid in fermentation liquor
  • Determination method of content of fulvic acid in fermentation liquor
  • Determination method of content of fulvic acid in fermentation liquor

Examples

Experimental program
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Embodiment 1

[0050] The mensuration of fulvic acid in present embodiment 1 molasses fermented liquid comprises the following steps:

[0051] 1. Prepare reagents:

[0052] 1.1 Sodium pyrophosphate extract: weigh 15g of sodium pyrophosphate and 7g of sodium hydroxide, dissolve in 1L of distilled water;

[0053] 1.2 Sulfuric acid solution: C ( 1 2 H 2 SO 4 ) = 2 mol / L ;

[0054] 1.3 O-phenanthroline-ferrous indicator: Weigh 1.490g of o-phenanthroline and dissolve it in 100ml aqueous solution containing 0.700g of ferrous sulfate, and store it in a brown bottle;

[0055] 1.4 Potassium dichromate solution: Weigh 49.031g of potassium dichromate, dissolve in 500ml of water (heat to dissolve if necessary), cool, dilute to 1L, and shake well;

[0056] 1.5 Po...

Embodiment 2

[0118] The determination of fulvic acid in the present embodiment 2 straw fermentation liquid comprises the following steps:

[0119] 1, prepare reagent: with embodiment 1;

[0120] 2. Sample solution preparation:

[0121] 2.1 Weigh 5.8403g of the straw fermentation broth sample, add about 30ml of sodium pyrophosphate extract, extract in a boiling water bath for 2 hours, take it out to cool, transfer to a 50ml volumetric flask, dilute to volume with sodium pyrophosphate extract, and filter. Suck 25.0ml of the filtrate into a 50ml beaker, and then inhale 25.0ml of the solution was stirred evenly and centrifuged. Aspirate three 5.0ml portions of the supernatant. A 250ml conical flask is inhaled for the determination of total fulvic acid;

[0122] 2.2 The other two were sucked into two 150ml beakers for the determination of sugar content. Add 2ml of HCl (1+1) to the solution, boil on the electric stove for 2-3 minutes, remove and cool. Use C(NaOH)=100g / L solution to adjust...

Embodiment 3

[0143] The mensuration of fulvic acid in the present embodiment 3 brewing fermented liquid comprises the following steps:

[0144] 1. Prepare reagents: same as Example 1.

[0145] 2. Sample solution preparation:

[0146] 2.1 Weigh 1.5383g of wine fermentation broth sample, add about 30ml of sodium pyrophosphate extract, extract in a boiling water bath for 2h, take it out and cool, transfer to a 50ml volumetric flask, dilute to volume with sodium pyrophosphate extract, and filter. Suck 25.0ml of the filtrate into a 50ml beaker, and then inhale 25.0ml of the solution was stirred evenly and centrifuged. Aspirate three 5.0ml portions of the supernatant. A 250ml conical flask is inhaled for the determination of total fulvic acid;

[0147] 2.2 The other two were sucked into two 150ml beakers for the determination of sugar content. Add 2ml of HCl (1+1) to the solution, boil on the electric stove for 2-3 minutes, remove and cool. Use C(NaOH)=100g / L solution to adjust to alkalin...

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Abstract

The invention discloses a determination method of content of fulvic acid in fermentation liquor. The method comprises the following steps of 1) determining sugar in the fermentation liquor; and 2) determining the total fulvic acid in the fermentation liquor; 3) calculating the content of pure fulvic acid in the fermentation liquor: a, calculating the content of glucose equivalent to fulvic acid according to the linear relationship that glucose is corresponding to fulvic acid; and b, mapping by taking the glucose mass as an x-coordinate and the corresponding fulvic acid mass as a y-coordinate, thereby obtaining a working curve and an equation of linear regression as Y=aX+b, wherein a refers to slope, and b refers to intercept, converting the content of sugar in a sample into the content of fulvic acid, and then subtracting the content of sugar which is converted into fulvic acid by the content of the total fulvic acid, and then dividing the total amount of the fermentation liquor sample, thereby obtaining the percentage composition of pure fulvic acid in the fermentation liquor. The method provided by the invention can accurately determine the content of fulvic acid in the fermentation liquor.

Description

Technical field: [0001] The invention relates to a method for determining the content of fulvic acid, in particular to a method for determining the content of fulvic acid in a fermented liquid. Background technique: [0002] The fermentation broth is produced by submerged liquid bio-fermentation technology of organic materials (plant residues) such as straw and bagasse wine fermentation alcohol. In addition to containing high concentrations of decomposed organic matter, humic acid, amino acids, high absorption rate N, P, K In addition, it also contains the unknown growth factor (UGF) produced during the fermentation process, which can stimulate plants similar to adding sodium nitrophenolate, naphthalene acetic acid and other synthetic hormones, and can be widely used as foliar fertilizer, liquid fertilization, Auxiliary raw material of solid organic granular fertilizer or directly diluted as finished foliage fertilizer and flush fertilization. Its effects are as follows: 1....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16
Inventor 侯勇曾芸夏中梅曾显斌黄秀辉朱彭玲陈浩王强锋张亮
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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