Pepper numb-taste component content detection based on method of quantitative analysis of multi-components by single marker
A technology of substance content and content, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no reference substance of prickly ash, no quantitative evaluation of prickly ash, etc.
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[0044] 1 instrument
[0045] Agilent 1260 high performance liquid chromatography, G1311C quaternary pump, G1329B autosampler, G1316A column thermostat, G1362A diode array detector (Agilent, USA); Waters e2695 high performance liquid chromatography, quaternary pump, 2998PDA detector ; Shimadzu Shimadzu LC-2010A, SIL-20A autosampler, SPD-20A detector, CTO-20A column thermostat, LC solution chromatographic workstation software (Shimadzu Corporation of Japan); BP211D electronic analytical balance (Germany Sartouris Co., Ltd. company).
[0046] 2 Materials and Reagents
[0047] Zanthoxylum bungeanum: Provided by Sichuan Hanyuan Huajiao Bureau.
[0048] Zanthoxylum bungeanum substance reference substance: hydroxy-α-sanshool (self-made, purity 98.35%); hydroxy-β-sanshool (self-made, has applied for national reference substance, purity 98.27%); , purity 98.54%).
[0049] Methanol: chromatographic grade, American Sigma Company. Acetonitrile: chromatographic grade, American Sigma C...
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