77 results about "Agro food" patented technology

Microspheres, having a size lower than 1mu and comprising a biocompatible polysaccharidic polymer, are prepared with a process comprising the precipitation of polymer induced by means of a supercritical antisolvent (SAS). These microspheres are used as vehicling agents or carriers in the preparation of pharmaceutical compositions administrable by oral, nasal, pulmonary, vaginal or rectal route. These microspheres can also be advantageously used as vehicling agent or carriers in the preparation of pharmaceutical compositions for the treatment of human diseases associated with genic defects, for the preparation of diagnostics and in the agro-alimentary industry.

The invention relates to a high-efficiency and low-cost preservation method of kiwi fruits, and in particular relates to a preservation method capable of combining microporous preservative films, 1-methylcyclopropene and a low-temperature storage technology of the kiwi fruits, belonging to the technical field of agricultural food science. Aiming at the characteristics of being easily softened and rotten in the storage process of the kiwi fruits, the aim of preservation can be achieved by methods such as modified atmosphere packaging, post-harvest physiological accommodation, control of pathogenic bacteria and low-temperature storage. The method comprises the steps of sorting the fresh kiwi fruits, putting the sorted fresh kiwi fruits into microporous freshness protection packages, fumigating by the 1-methylcyclopropene, fumigating by a preservation smoke agent, precooling, sealing the packages, and storing in a fresh-keeping storehouse with the temperature of 0+ / -1 DEG C and the humidity of more than or equal to 80%; after 90 days, fumigating by the preservation smoke agent once every 30 days, and therefore, the kiwi fruits can be effectively stored for 180 days, and have the hardness of more than or equal to 4kg / m<2> and the edible rate of more than or equal to 90%.

The invention discloses a recombinant alginate lyase and a construction method and an application thereof, which belong to the technical field of biology. The alginate lyase Aly-Cob is derived from anenvironment sample (a mixture of sea-tangle stacking place and factory production waste material). By using a gene engineering technology, a gene of the alginate lyase is cloned to an expression vector, and a recombinant bacterial strain for heterogenous expression of the alginate lyase is obtained. The alginate lyase Aly-Cob produced by the bacterial strain through fermentation has the functionfor degrading sodium alginate to prepare alginate oligosaccharide. The provided alginate lyase Aly-Cob can be widely used for the fields of agriculture, food, feed addition, medicine and alga processing.

The present invention relates to a process for preparing an edible foodstuff from food industry waste stream byproducts. This process involves the steps of: (i) combining, in an extruder, an extrusion formulation comprising a first food byproduct and at least one additional ingredient; (ii) introducing supercritical carbon dioxide (SC—CO2) into the extruder to mix with the first food byproduct and the at least one additional ingredient; and (iii) producing an edible foodstuff containing the first food byproduct and the at least one additional ingredient, where the edible foodstuff comprises an extrudate prepared under supercritical fluid extrusion (SCFX) conditions. The present invention also relates to a process for preparing an edible foodstuff from a protein concentrate. The present invention further relates to edible foodstuffs produced by the various processes disclosed herein.

The method is characterised in that the thermal energy necessary for thermolysis of the waste carried out in the absence of air is provided by a heating mass, comprising steel balls (9), running in the furnace (1) co-currently with the waste. The method may be applied to household waste, sewage work residues, hospital wastes, wastes of risk to the agro-food industry and in a general manner to all wastes containing organic matter be it of urban, agricultural or industrial origin.

The disclosure relates to a method for inspection of the quality of products produced by a production system, by monitoring the exhaustiveness, effectiveness and reliability of a traceability system associated with said production system. The method includes the following steps: determination of a plurality of domains of said traceability system to be inspected; determination of a plurality of criteria to be monitored for each of said domains, said criteria for a domain being correlated to each other; for each of said criteria, assignment of a numerical data representing a quality value by measurement through at least one sensor and / or by assessment; for each of said domains, multiplication of all said numerical data for each said criterion in said domain and application of a mathematical regression function to the resulting product to obtain a numerical data representative of a quality value of said domain; comparison of said numerical data with at least one predetermined threshold for each of said domains.

The present invention relates to a method for preparing a cyclodextrin polymer and / or a hydrophilic polymer emulsion having cyclodextrins as well as lipophilic compounds, the emulsions having a remarkable stability. In particular, the method includes: (i) adding a lipophilic compound into an aqueous solution of a cyclodextrin unit polymer or hydrophilic polymer having cyclodextrins; (ii) forming an emulsion from the mixture resulting from step (i). The invention also relates to the resulting emulsions, i.e. stabilized emulsions by a non-covalent and non-crystalline inclusion complex consisting of (i) a cyclodextrin unit polymer or a hydrophilic polymer having cyclodextrins and (ii) a lipophilic compound. The invention also relates to the use of said emulsions in the cosmetic, pharmaceutical and / or agri-food fields.

The invention relates to a water adsorption material, particularly relates to an acid heat treatment attapulgite water quality purifying agent. Attapulgite has a special fibrous structure, unusual colloid and adsorption performance, is wide in application field and has good reputation of earth king. The attapulgite clay has a special chained crystal structure, good colloid properties such as unique dispersion, water absorption and salt and alkali resistance and relatively high adsorption decoloring capacity and has good plasticity and adhesion performance, thereby being widely applied in various industries such as petroleum, chemical industry, building materials, papermaking, medicine, agriculture, environmental protection, printing and articles for daily use. Since the attapulgite crystal fibers are slender, internally porous, large in specific surface area and good in selection adsorption performance, the attapulgite is widely applied to the fields such as chemical industry, agriculture, food, medicine and environmental protection. At present, the attapulgite is widely used for the sewage treatment and particularly used for treating the waste water in printing and dyeing mills and tanneries, and the treated sewage can reach the national emission standard.

The invention discloses a gene of endo-cellulase derived from Paenibacilluspolymyxa as well as preparation and an application of the endo-cellulase. The gene of the endo-cellulase is cloned on an escherichia coli expression vector according to the technical method of genetic engineering to obtain an escherichia coli recombination strain capable of heterologously expressing the endo-cellulase, andthe endo-cellulase prepared through the heterologous expression of the strain effectively degrades konjac glucomannan (also known as konjac glucomannan). The provided endo-cellulase can be widely applied to the fields of preparation of feed additives, medicines and oligo-glucomannan in agriculture and foods.

The invention relates to preparation methods for a rare-earth terbium complex and rare-earth terbium complex fluorescent gel. The method comprises the steps: firstly synthesizing the rare-earth terbium complex, i.e., Tb(ACAC)3.2H2O which serves as luminescent molecules; and then, introducing the rare-earth terbium complex into a PVA matrix, and forming the fluorescent gel in the presence of a cross-linking agent. According to the preparation method for the fluorescent gel, high rare-earth doping concentration can be obtained, and the fluorescence intensity is improved. The preparation method is simple, is high in operability and short in reaction time, is environment-friendly and is applicable to industrial production. Compared with the traditional products, the product prepared by the preparation method has the advantages of good biocompatibility, degradability, low toxicity, good chemical stability and the like and has a broad application prospect in the fields of biomaterials, medical treatment, agriculture, food and the like.

The invention belongs to the field of molecular biology and particularly relates to chitosanase Csncv and a mutant CsnB and application of the chitosanase Csncv. The mutant CsnB is obtained by mutating the chitosanase Csncv after codon optimization, an amino acid sequence and a nucleotide sequence of the optimized chitosanase Csncv are shown as SEQ ID NO.16-17, and an amino acid sequence and a nucleotide sequence of the mutant CsnB are shown as SEQ ID NO.1-14. By constructing an efficient-expression engineering strain of the CsnB, the thermal stability of the chitosanase is improved, and chitosan is hydrolyzed to obtain a chitosan oligosaccharide product containing chitobiose, chitotriose, chitotetraose, chitopentaose and chitohexaose; and the chitosanase Csncv and the mutant CsnB of the chitosanase Csncv can be applied to preparation of chitosan oligosaccharides and industries such as medicine, agriculture and food, and a foundation is laid for industrial application of the chitosanase.

The invention discloses a preparation method and an application of a glucomannanase gene derived from Klebsiella oxytoca and an enzyme thereof, which relates to a technical method using genetic engineering. The gene of the glucomannanase is cloned into an E. coli expression vector to obtain a recombinant strain of Escherichia coli which can heterologously express the enzyme. The glucomannanase prepared by heterologous expression of the strain can efficiently degrade konjac polysaccharide (also known as konjac glucomannan). The glucomannanase provided by the invention can be widely applied to the fields of agriculture, food, feed additives, medicine and preparation of glucomannan oligosaccharides.

The invention relates to a method for extracting, separating and purifying anthocyanin from blueberry peel and residues and belongs to the technical field of agriculture and food science. Effective ingredients are separated and extracted from fruit processing waste residues, so the method is also relevant to three-waste utilization. According to the method, edible alcohol is used for extracting anthocyanin crude extracts from blueberry peel and residues at 45 to 50 DEG C, and in addition, AB-8 macroporous resin is used for separation and purification. The method provided by the invention has the characteristics that the technology is novel, the operation and the production are simple and convenient, the low cost is low, the yield is high, the environment-friendly effect is realized; and the utilization rate of blueberry fruits is improved, the content of the anthocyanin in the separated product is greater than or equal to 26 percent, the requirements of various function food, health care food, medicine and food additives on the anthocyanin are met, and wide purposes are realized.

The invention relates to a preservation method for table blueberries, particularly relates to a preservation method for increasing the content of calcium ions in fruit cell walls by spraying blueberry fresh fruits through organic calcium (sugar alcohol chelated calcium), and belongs to the technical field of agricultural food science. Aiming at the disadvantage that the fresh blueberry fruits are very easy to soften so as to be rotted in a storage process, the preservation prolonging aim is realized by the method for increasing the content of the calcium ions in the fruit cell walls and inhibiting the cell separation. A sugar alcohol chelated calcium liquid fertilizer is used for carrying out fixed-period spraying (15 times per day) on the fruit-setting blueberry fruits; then the fresh fruits are picked, sorted and put into a small polyethylene (PE) box (150 grams per box), and then the boxes are contained in a micro-pore preservation bag (10 boxes per bag); then the bags are tied after the fresh fruits are pre-cooled at 0+ / -1 DEG C for 24 hours to be stored under the condition of -1 DEG C.

The invention discloses a fresh-keeping method for prolonging the shelf life of fresh beans, and belongs to the technical field of agricultural food science. According to the fresh-keeping method, the aim of keeping fresh is fulfilled by inhibiting diseases by adopting spontaneous modified atmosphere packaging, a postharvest physiological regulator and a low-temperature storage method to overcome the quality deterioration characteristics of rusty spots, water loss, meat hardening, fibrosis, rot and the like which are easily caused in the storage process of fresh beans. The fresh beans are pre-cooled to reduce field heat after being harvested and are sorted to remove beans with mechanical injuries, defects, diseases and pests, and then the left beans are stored into PE (poly ethylene) preservation bags with the thickness of 20 to 40 microns; 1-MCP (methyl cyclopentenolone) with the equivalent concentration of 0.5 to 2.0 micro-liter / L is added into the preservation bags; the preservation bags are bundled and fumigated at 15 to 20 DEG C for 2 to 4 hours, and then are opened; the fresh beans are pre-cooled for 24 to 48 hours in a fresh-keeping storehouse with the temperature of 10+ / -1 DEG C and the relative humidity of greater than or equal to 85 percent, and then are stored in the fresh-keeping storehouse with the temperature of 9+ / -1 DEG C and the relative humidity of greater than and equal to 85 percent. The fresh-keeping method for prolonging the shelf life of fresh beans is high in efficiency, convenient, and easy to operate.

The present invention discloses a polysaccharide cleavage monooxygenase encoding gene and an enzyme thereof derived from myceliophthora thermophila, and a preparation method and applications. A genetic engineering technical method is used to clone the gene of the polysaccharide cleavage monooxygenase into pichia pastoris expression vectors to obtain a pichia pastoris recombinant strain capable ofheterologously expressing the enzyme. The polysaccharide cleavage monooxygenase heterologously expressed by the strain can efficiently degrade cellulose (oxidative cleavage of beta-1,4-glycosidic bonds). The provided polysaccharide cleavage monooxygenase can be widely applied to the fields of energy, agriculture, food, feed additives, medicines, etc.

The invention relates to a functional nano SiO2 / polypropylene carbonate composite material and a preparation method therefor. In the composite material, a mas ratio of the polypropylene carbonate to the functional nano SiO2 is 100 to (0.5-6); and the composite material is prepared by fusing and blending the polypropylene carbonate and the functional nano SiO2. The functional nano SiO2 is prepared by virtue of an in-situ modifying technology. The functional nano SiO2 / polypropylene carbonate composite material provided by the invention has relatively good comprehensive performance, tensile strength of 17-37MPa, a glass-transition temperature of 30-39 DEG C, a maximum thermal decomposition temperature of 279-296 DEG C and elongation at break higher than 200%. A film prepared by the composite material has relatively good light transmission, so that the applied range, in the agricultural field, the food field and the packaging field, of the polypropylene carbonate is expanded; and the preparation method for the composite material is simple, and convenient for popularization and use.

The invention discloses an endo-chitosan enzyme CsnW2 gene sequence and preparation method and application thereof. The invention relates to a soil new isolated bacterial strain Aspergillus clavatus from the endo-chitosan enzyme CsnW2. The invention also provides a method for preparing the novel chitosan enzyme, ie a gene engineering technique, which includes the following steps: cloning the novel chitosan enzyme gene to a pichia yeast expression vector, and obtaining a pichia yeast recombination bacterial strain for heterogenous express of the enzyme. The chitosan enzyme CsnW2 prepared by heterogenous expression of the bacterial strain has high activity at pH 3.5-5.0 and 40-60 DEG C. with Chitosan oligosaccharide preparation function by chitosan degradation. The chitosan enzyme CsnW2 provided by the invention can be widely applied to the chemical industry, the agriculture, the foodstuff, the forage adding, the medicine and other fields, and has large production potential and economic value.

The present invention relates to an ultra-high speed real-time PCR device on the basis of a lab-on-a-chip for detecting bacteria that causes food poisoning pertaining to agricultural food and a food poisoning detection method using the same. The present invention can provide a micro PCR chip which can simultaneously accommodate a plurality of small-volume samples and concurrently secure maximum thermal contact efficiency with a heating block so as to secure rapid results, and accurately measure an optical signal emitted from a nucleic acid amplification product even without any separate filtering or processing. Further, on the basis of the PCR chip, the present invention can provide a real-time PCR device which can rapidly obtain a nucleic acid amplification result of which the reliability is secured even without a complicated light-signal measuring module.

The invention discloses a k-carrageenase gene derived from Pedrobacter hainanensis Q-13, a preparation method of its enzyme and an application thereof, a genetic engineering technical method is used to clone the gene of the k-carrageenase to an E. coli expression vector to obtain a recombinant strain of Escherichia coli which can heterologously express the enzyme. The k-carrageenase prepared by heterogenous expression of the strain can efficiently degrade k-carrageenan. The k-carrageenase provided by the invention can be widely used in the fields of agriculture, food, feed additives, medicine,cosmetics and carrageenan oligosaccharide preparation.

The invention discloses an alginate lyase mutant gene, an alginate lyase mutant, an engineering bacterium containing the alginate lyase mutant as well as a construction method and application. The invention further discloses a technical method of genetic engineering, the mutant gene of the novel alginate lyase is cloned to an escherichia coli expression vector to obtain an escherichia coli recombinant strain capable of heterologously expressing the alginate lyase, and the alginate lyase mutant mAlgNJU-03 prepared by heterologously expressing the strain has the functions of degrading sodium alginate to prepare sodium alginate oligosaccharide and changing the polymerization degree of the oligosaccharide. The alginate lyase mutant mAlgNJU-03 provided by the invention can be widely applied to the fields of chemical engineering, agriculture, food, feed additives, medicines, seaweed genetic engineering and the like.

A mobile laboratory and method to expedite regulatory test results for agricultural food and / or beverage products. The mobile laboratory can include test equipment mounted on shock absorbing and leveling stands that are disposed in a truck or in a trailer. The mobile laboratory can be taken directly to a customer's location and tests performed on samples at that location, thus avoiding the time and expense of the customer packaging and shipping samples to a non-mobile lab, thus providing the ability to deliver test results directly to the customer much more quickly. The mobile laboratory can include one or more gas chromatography, mass spectroscopy, and / or liquid chromatography systems.

The invention relates to a tissue form stabilizer for kiwi fruit and blueberry juice drinks and belongs to the technical field of agricultural food science. The tissue form stabilizer is characterized by comprising one or more of sodium carboxymethylcellulose, propylene glycol alginate fat, sucrose ester and D-sodium erythorbate. The tissue form stabilizer effectively solves the problem of the unstable tissue form of the kiwi fruit and blueberry mixed juice drinks. The stabilizer, powdered sugar and water are ground into collagen liquid through colloid, and secondary homogeneity, disinfection and filling are performed on the collagen liquid, fruit pulp and water to obtain products. The products obtained by the method are fadeless within shelf time, have evenly-distributed pulp, and are free of precipitation and layering.

The invention discloses a mannuronan C-5 epimerase / alginate lyase encoding gene, and a mannuronan C-5 epimerase / alginate lyase enzyme and preparation and application thereof, belonging to the field ofgene engineering. The invention particularly discloses an enzyme gene which is derived from pseudomonas mendocina and has two functions of epimerizing mannuronan C-5 and cracking alginate, and a preparation method and application of the enzyme of the enzyme gene. According to the invention, a gene of the enzyme is cloned to an escherichia coli expression vector by utilizing a technical method ofgenetic engineering to obtain a recombinant escherichia coli strain capable of heterologously expressing the enzyme, and the enzyme prepared by heterologously expressing the strain can efficiently convert beta-D-mannuronan (M) into alpha-L-guluronate (G) and crack sodium alginate into oligosaccharide. The bifunctional enzyme provided by the invention can be widely applied to the fields of agriculture, food, feed additives, medicines and the like.

The invention discloses a strain of Flavobacter johniii producing alginate lyase, which belongs to the field of biotechnology. The present invention isolates Flavobacterium johnsoniae WX-11 from soil and river water samples, that is, Flavobacterium johnsoniae CGMCC No.14444. The strain has simple nutritional requirements and short fermentation time, and is used in the production of alginate lyase The production cycle can be significantly shortened and the production cost can be reduced. The invention also discloses the use of the strain to prepare alginate lyase and reduce the viscosity of high-viscosity raw materials containing alginate. The alginate lyase produced by the Flavobacterium johniiii WX-11 provided by the present invention can degrade sodium alginate to generate biologically active fucoidan oligosaccharides, which can be widely used in the fields of agriculture, food, feed addition, medicine and seaweed processing, etc. .

The invention belongs to the technical field of agricultural food science, and particularly relates to prevention and treatment of cold damage in the postharvest storage process of pitaya. The preparation method specifically comprises the following steps: (1) selecting pitaya which is not damaged in appearance and does not turn red completely; (2) spraying a liquid medicine A 3-7 days before harvesting; (3) spraying a liquid medicine B after harvesting; (4) boxing; (5) storing at normal temperature for 6-8 hours; (6) spraying the liquid medicine B again; (7) disinfecting a refrigeration house;and (8) cooling in sequence. According to the method, peel yellowing and fruit rotting caused by cold damage and pathogenic bacteria in the low-temperature storage process of the pitaya can be obviously inhibited; the moisture loss of the pitaya is reduced, the hardness and the nutrient content of the pitaya are kept, the postharvest life and quality of the pitaya can be effectively prolonged, the original quality of the fresh pitaya preserved by the method of the invention at low temperature can still be kept on the 31st day, and the good fruit rate reaches 97% or above.

The invention discloses a polypeptide having deepoxidation catalytic activity, and encoding nucleic acid and application thereof. The polypeptide provided by the invention can catalyze a reaction of trichothecene mycotoxins and glutathione under mild conditions to remove epoxy groups and generate non-toxic and harmless glutathione derivatives, so that detoxification and detoxification of trichothecene mycotoxins are realized. The polypeptide has wide applications in the fields of agriculture, food, feed, medicine and the like.

The present invention relates to a novel peptide that exhibits effective antimicrobial activity against various gram-positive and gram-negative bacteria that are involved in food-borne pathogenesis, food spoilage and other pathogenic conditions. Therefore, this peptide can be a good candidate as antibacterial in agricultural, food and beverage industry, as well as for other medical applications and societal use.

The invention discloses a cordate houttuynia preservation method, in particular relates to a method for preserving cordate houttuynia by using gas packing, belonging to the technical field of agricultural food science. The method aims at the characteristics that cordate houttuynia is subjected to brown stain easily, and the brittleness of the cordate houttuynia is reduced in the cordate houttuynia storage process, and can achieve the preservation by using the gas packing method. The method comprises the steps of cleaning the cordate houttuynia, blowing away part of waste heat, pre-cooling, then placing the cordate houttuynia into ethylene / ethylene vinyl alcohol copolymer freshness protection bags, filling a gas formed by mixing CO2, N2 and O2, carrying out heat sealing, and placing the cordate houttuynia in a refrigeration house being 2+ / -1 DEG C for storage. The mixed gas comprises the gases of 20-30% of the CO2, 5-10% of the O2 and the balance being the N2 according to the volume rate. The retain freshness gas mixing accuracy error rate is less than 1.5%; the cordate houttuynia can be stored for 30 days; the edible rate of the cordate houttuynia is more than or equal to 85%.