75 results about "Agro food" patented technology

The disclosure relates to a method for inspection of the quality of products produced by a production system, by monitoring the exhaustiveness, effectiveness and reliability of a traceability system associated with said production system. The method includes the following steps: determination of a plurality of domains of said traceability system to be inspected; determination of a plurality of criteria to be monitored for each of said domains, said criteria for a domain being correlated to each other; for each of said criteria, assignment of a numerical data representing a quality value by measurement through at least one sensor and / or by assessment; for each of said domains, multiplication of all said numerical data for each said criterion in said domain and application of a mathematical regression function to the resulting product to obtain a numerical data representative of a quality value of said domain; comparison of said numerical data with at least one predetermined threshold for each of said domains.

The invention belongs to the field of molecular biology and particularly relates to chitosanase Csncv and a mutant CsnB and application of the chitosanase Csncv. The mutant CsnB is obtained by mutating the chitosanase Csncv after codon optimization, an amino acid sequence and a nucleotide sequence of the optimized chitosanase Csncv are shown as SEQ ID NO.16-17, and an amino acid sequence and a nucleotide sequence of the mutant CsnB are shown as SEQ ID NO.1-14. By constructing an efficient-expression engineering strain of the CsnB, the thermal stability of the chitosanase is improved, and chitosan is hydrolyzed to obtain a chitosan oligosaccharide product containing chitobiose, chitotriose, chitotetraose, chitopentaose and chitohexaose; and the chitosanase Csncv and the mutant CsnB of the chitosanase Csncv can be applied to preparation of chitosan oligosaccharides and industries such as medicine, agriculture and food, and a foundation is laid for industrial application of the chitosanase.

The invention relates to a method for extracting, separating and purifying anthocyanin from blueberry peel and residues and belongs to the technical field of agriculture and food science. Effective ingredients are separated and extracted from fruit processing waste residues, so the method is also relevant to three-waste utilization. According to the method, edible alcohol is used for extracting anthocyanin crude extracts from blueberry peel and residues at 45 to 50 DEG C, and in addition, AB-8 macroporous resin is used for separation and purification. The method provided by the invention has the characteristics that the technology is novel, the operation and the production are simple and convenient, the low cost is low, the yield is high, the environment-friendly effect is realized; and the utilization rate of blueberry fruits is improved, the content of the anthocyanin in the separated product is greater than or equal to 26 percent, the requirements of various function food, health care food, medicine and food additives on the anthocyanin are met, and wide purposes are realized.

The invention relates to a preservation method for table blueberries, particularly relates to a preservation method for increasing the content of calcium ions in fruit cell walls by spraying blueberry fresh fruits through organic calcium (sugar alcohol chelated calcium), and belongs to the technical field of agricultural food science. Aiming at the disadvantage that the fresh blueberry fruits are very easy to soften so as to be rotted in a storage process, the preservation prolonging aim is realized by the method for increasing the content of the calcium ions in the fruit cell walls and inhibiting the cell separation. A sugar alcohol chelated calcium liquid fertilizer is used for carrying out fixed-period spraying (15 times per day) on the fruit-setting blueberry fruits; then the fresh fruits are picked, sorted and put into a small polyethylene (PE) box (150 grams per box), and then the boxes are contained in a micro-pore preservation bag (10 boxes per bag); then the bags are tied after the fresh fruits are pre-cooled at 0+ / -1 DEG C for 24 hours to be stored under the condition of -1 DEG C.

The invention discloses a fresh-keeping method for prolonging the shelf life of fresh beans, and belongs to the technical field of agricultural food science. According to the fresh-keeping method, the aim of keeping fresh is fulfilled by inhibiting diseases by adopting spontaneous modified atmosphere packaging, a postharvest physiological regulator and a low-temperature storage method to overcome the quality deterioration characteristics of rusty spots, water loss, meat hardening, fibrosis, rot and the like which are easily caused in the storage process of fresh beans. The fresh beans are pre-cooled to reduce field heat after being harvested and are sorted to remove beans with mechanical injuries, defects, diseases and pests, and then the left beans are stored into PE (poly ethylene) preservation bags with the thickness of 20 to 40 microns; 1-MCP (methyl cyclopentenolone) with the equivalent concentration of 0.5 to 2.0 micro-liter / L is added into the preservation bags; the preservation bags are bundled and fumigated at 15 to 20 DEG C for 2 to 4 hours, and then are opened; the fresh beans are pre-cooled for 24 to 48 hours in a fresh-keeping storehouse with the temperature of 10+ / -1 DEG C and the relative humidity of greater than or equal to 85 percent, and then are stored in the fresh-keeping storehouse with the temperature of 9+ / -1 DEG C and the relative humidity of greater than and equal to 85 percent. The fresh-keeping method for prolonging the shelf life of fresh beans is high in efficiency, convenient, and easy to operate.

The present invention discloses a polysaccharide cleavage monooxygenase encoding gene and an enzyme thereof derived from myceliophthora thermophila, and a preparation method and applications. A genetic engineering technical method is used to clone the gene of the polysaccharide cleavage monooxygenase into pichia pastoris expression vectors to obtain a pichia pastoris recombinant strain capable ofheterologously expressing the enzyme. The polysaccharide cleavage monooxygenase heterologously expressed by the strain can efficiently degrade cellulose (oxidative cleavage of beta-1,4-glycosidic bonds). The provided polysaccharide cleavage monooxygenase can be widely applied to the fields of energy, agriculture, food, feed additives, medicines, etc.

The invention discloses a k-carrageenase gene derived from Pedrobacter hainanensis Q-13, a preparation method of its enzyme and an application thereof, a genetic engineering technical method is used to clone the gene of the k-carrageenase to an E. coli expression vector to obtain a recombinant strain of Escherichia coli which can heterologously express the enzyme. The k-carrageenase prepared by heterogenous expression of the strain can efficiently degrade k-carrageenan. The k-carrageenase provided by the invention can be widely used in the fields of agriculture, food, feed additives, medicine,cosmetics and carrageenan oligosaccharide preparation.

The invention relates to a tissue form stabilizer for kiwi fruit and blueberry juice drinks and belongs to the technical field of agricultural food science. The tissue form stabilizer is characterized by comprising one or more of sodium carboxymethylcellulose, propylene glycol alginate fat, sucrose ester and D-sodium erythorbate. The tissue form stabilizer effectively solves the problem of the unstable tissue form of the kiwi fruit and blueberry mixed juice drinks. The stabilizer, powdered sugar and water are ground into collagen liquid through colloid, and secondary homogeneity, disinfection and filling are performed on the collagen liquid, fruit pulp and water to obtain products. The products obtained by the method are fadeless within shelf time, have evenly-distributed pulp, and are free of precipitation and layering.

The invention discloses a strain of Flavobacter johniii producing alginate lyase, which belongs to the field of biotechnology. The present invention isolates Flavobacterium johnsoniae WX-11 from soil and river water samples, that is, Flavobacterium johnsoniae CGMCC No.14444. The strain has simple nutritional requirements and short fermentation time, and is used in the production of alginate lyase The production cycle can be significantly shortened and the production cost can be reduced. The invention also discloses the use of the strain to prepare alginate lyase and reduce the viscosity of high-viscosity raw materials containing alginate. The alginate lyase produced by the Flavobacterium johniiii WX-11 provided by the present invention can degrade sodium alginate to generate biologically active fucoidan oligosaccharides, which can be widely used in the fields of agriculture, food, feed addition, medicine and seaweed processing, etc. .

The invention relates to a preservation method for table blueberries, particularly relates to a preservation method for increasing the content of calcium ions in fruit cell walls by spraying blueberry fresh fruits through organic calcium (sugar alcohol chelated calcium), and belongs to the technical field of agricultural food science. Aiming at the disadvantage that the fresh blueberry fruits are very easy to soften so as to be rotted in a storage process, the preservation prolonging aim is realized by the method for increasing the content of the calcium ions in the fruit cell walls and inhibiting the cell separation. A sugar alcohol chelated calcium liquid fertilizer is used for carrying out fixed-period spraying (15 times per day) on the fruit-setting blueberry fruits; then the fresh fruits are picked, sorted and put into a small polyethylene (PE) box (150 grams per box), and then the boxes are contained in a micro-pore preservation bag (10 boxes per bag); then the bags are tied after the fresh fruits are pre-cooled at 0+ / -1 DEG C for 24 hours to be stored under the condition of -1 DEG C.