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31results about How to "Solve the problem of high production cost" patented technology

Incision beta-1,3-glucanase coding gene, as well as enzyme, preparation method and application thereof

The invention discloses an incision beta-1,3-glucanase coding gene from cellulosimicrobium cellulans, as well as enzyme, a preparation method and application of the incision beta-1,3-glucanase coding gene. The incision beta-1,3-glucanase coding gene is cloned to an escherichia coli expression vector through a technical method of gene engineering, so that an escherichia coli recombination strain capable of realizing heterologous expression of the enzyme can be obtained; incision beta-1,3-glucanase prepared through the heterologous expression of the strain can directly and efficiently degrade solid Curdlan. The invention further provides an antibiological inoculant, which is the incision beta-1,3-glucanase, for preventing and treating animal and plant diseases; the antibiological inoculant belongs to the field of biological prevention and treatment, and is expected to be novel biological pesticide. The incision beta-1,3-glucanase provided by the invention can be widely applied to the field of agriculture, food, feed additives, medicines, glucooligosaccharides preparation and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Synthesis process of battery-grade iron phosphate

PendingCN110540185AEliminate response time inhomogeneitiesEnsure Stability and ConsistencyCell electrodesPhosphorus compoundsChemistryParameter control
The invention discloses a synthesis process of battery-grade iron phosphate. The process comprises impurity removal and purification of ferrous sulfate and synthesis of iron phosphate. At least one ofthe following problems is solved: 1, the difficult problem of high production cost of iron phosphate at present is solved by using titanium dioxide by-product ferrous sulfate as a raw material; 2, full parameter control of a production process is realized, so that disadvantages existing in a traditional process, such as an uneven reaction time and change of a reaction solution pH value, are eliminated, and thereby the size and shape of obtained iron phosphate crystals is consistent, stability and consistency of a product are fully guaranteed, and product differences between batches are small;and 3, impurities are less, and high quality of the product is realized.
Owner:襄阳泽东新能源发展有限公司

Composite catalytic solid-phase prepared hydroxypropyl starch and preparing method therefor

The invention relates to hydroxypropyl starch and the manufacture method. It is made from starch 1 portion, propylene oxide 0.2-0.8 portion, fatty alcohol polyethenoxy ether 0.06-0.15 portion, NaOH 0.03-0.06 portion, and water 0.12-0.3 portion. The method could obviously improve the reacting speed, and the product could meet the demand of paper industry without neutralization.
Owner:NORTHWEST NORMAL UNIVERSITY

K-carrageenase encoding gene and preparation and application thereof

The invention discloses a k-carrageenase gene derived from Pedrobacter hainanensis Q-13, a preparation method of its enzyme and an application thereof, a genetic engineering technical method is used to clone the gene of the k-carrageenase to an E. coli expression vector to obtain a recombinant strain of Escherichia coli which can heterologously express the enzyme. The k-carrageenase prepared by heterogenous expression of the strain can efficiently degrade k-carrageenan. The k-carrageenase provided by the invention can be widely used in the fields of agriculture, food, feed additives, medicine,cosmetics and carrageenan oligosaccharide preparation.
Owner:中科绿帅生物科技(广州)有限公司

Fabricated beam-column connecting joint

The invention relates to the technical field of beam-column connecting joints, in particular to a fabricated beam-column connecting joint. The fabricated beam-column connecting joint comprises a U-shaped bracket and a prefabricated laminated beam connecting joint, the periphery of a U-shaped steel skeleton is wrapped with prefabricated concrete; and the prefabricated laminated beam connecting joint comprises a connecting web plate, an upper flange plate and a lower flange plate and is connected with a structural beam, the upper flange plate and a fixing plate are connected and fixed by inserting bolts into second bolt holes and first bolt holes, and web plate studs and bracket studs are arranged in a staggered mode. According to the fabricated beam-column connecting joint, by combining with the steel structure industry with highly-developed industrialization and cooperating with a reinforced concrete combination technology, a system is innovated, the problems of inherent deficiencies of assembling of reinforced concrete connecting joints, environmental pollution of on-site construction, application of green building materials, high production cost, and supply side reform de-capacity are solved, and great significance is achieved.
Owner:BEIJING ZHIHUI YUNJIAN TECH CO LTD

Method for producing baohuoside I through herba epimdii

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.
Owner:XICHANG DANYANG BIOLOGY TECH CO LTD

Double-arm robot

The invention provides a double-arm robot. The double-arm robot comprises a base, a speed reducing mechanism assembled on the base and two mechanical arms. The speed reducing mechanism synchronously drives the two mechanical arms to rotate. The two mechanical arms are detachably and fixedly connected with the speed reducing mechanism. The included angle between the two mechanical arms is adjustable. It can be seen from the description that the two mechanical arms are installed on one speed reducing mechanism, the usage amount of speed reducers is reduced, and therefore the manufacturing cost is reduced; and the included angle between the two mechanical arms can be adjusted, multi-angle and multi-station application can be achieved in combination with different application program point positions, and the production efficiency is improved.
Owner:GREE ELECTRIC APPLIANCES INC

Beta-1,4-glucanase encoding gene and preparation method and application thereof

The invention discloses a preparation method and application of internally tangent beta-1,4-glucanase gene derived from paenibacilluspolymyxa and its enzyme. The preparation method is a technique method utilizing genetic engineering. The internally tangent beta-1,4-glucanase gene is cloned to an escherichia coli expression vector to obtain an escherichia coli recombination strain which can heterologously express the enzyme, and the strain heterologously expresses the prepared internally tangent beta-1,4-glucanase and can efficiently degrade konjac glucomannan (also known as konjac glucomanna).The prepared internally tangent beta-1,4-glucanase can be widely applied the fields of preparation of agriculture, foods, feed additives, medicine, oligo-glucomannan and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Parameter determination method and system for microgrid simulation model

The embodiment of the invention discloses a parameter determination method and system for a microgrid simulation model. The method comprises the following steps: carrying out a test on a sub-module ofequipment to be tested to determine test data, carrying out parameter identification according to the test data, generating an identification parameter set, carrying out a verification test on a microgrid according to a preset verification test scheme, and determining verification test data, wherein the micro-grid is composed of a plurality of equipment to be tested, the same simulation test is carried out on the micro-grid simulation model according to the verification test carried out on the micro-grid based on the identification parameter set to obtain the simulation test data of the micro-grid, and the parameters of the micro-grid simulation model are adjusted according to the verification test data and the simulation test data. According to the technical scheme provided by the embodiment of the invention, the problem of relatively high production cost of the microgrid due to the fact that input parameters and output parameters of different electrical equipment of the microgrid are difficult to consider in an existing parameter determination method for the simulation model of the microgrid is solved.
Owner:HEFEI SUNGROW RENEWABLE ENERGY SCI & TECH CO LTD

Manufacturing technology and structure of novel automobile partition wall

The invention discloses a manufacturing technology and a structure of a novel automobile partition wall. The manufacturing technology comprises the steps that a layer of first plate, two layers of second plates and two layers of third plates are combined into a wall body; the wall body and a metal insert are put into a mold; the mold is put into a baking machine to be preheated; the mold is put ina hot press for heat preservation and pressure maintaining, and then the wall body and the metal insert are subjected to punch forming; and after cooling, the mold is opened to take out a workpiece,and edge cutting process treatment is carried out. According to the manufacturing technology, the metal insert needing to be assembled is directly placed in the mold to be positioned, then the wall body with good mechanical property is clamped on the mold, the wall body and the metal insert are directly and fixedly connected together after preheating and hot pressing to finish assembling, and a large number of welding and assembling procedures do not need to be carried out after a stamped metal piece is produced, so that the manufacturing time of the partition wall is greatly saved, and the production cost can be reduced.
Owner:凯勒(南京)新材料科技有限公司

An endo-β-1,3-glucanase coding gene, enzyme, preparation method and application thereof

The invention discloses an endo-β-1,3-glucanase coding gene derived from Cellulosimerbium cellsulans and its enzyme, preparation method and application. The gene of the endo-β-1,3-glucanase is cloned into an Escherichia coli expression vector to obtain a recombinant E. coli strain capable of expressing the enzyme heterologously, and the endo-β-1,3-glucanase prepared by heterologous expression of the strain is obtained. 3‑glucanase, which can directly and efficiently degrade solid Curdlan (codran polysaccharide). The invention also provides a biocontrol agent for preventing and controlling animal and plant diseases—endo-β-1,3-glucanase, which belongs to the field of biological control and is expected to become a new type of biological pesticide. The endo-β-1,3-glucanase provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine, glucooligosaccharide preparation and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Highly expressed recombinant gutted adenovirus containing human constant region whole antibody gene and its use

The invention has supplied a recombinant virus containing whole antibody gene of the human constant zone with highly effective expression and its application. By bringing in the nucleotide sequence containing whole antibody gene of the human constant zone into the recombinant gene group, the invention has expressed the whole antibody of human constant zone in cell or body with high efficiency for curing illness.
Owner:钱其军

Touch panel

A touch panel including plural electrodes is provided. The plural electrodes form a sensing region and are disposed on the same plane. The sensing region has a first border and a second border substantially perpendicular to each other. The electrodes include one parallelogram electrode. The parallelogram electrode has two first edges parallel to the first border and two second edges parallel to neither the first border nor the second border.
Owner:MSTAR SOFTWARE R&D (SHENZHEN) LTD +1

Pressing die and pressing method for vinylidene fluoride impeller with movable ring

The invention belongs to the technical field of water pumps, and discloses a pressing die and a pressing method for a vinylidene fluoride impeller with a movable ring. The invention aims to solve theproblem in the production of the vinylidene fluoride impeller with the movable ring in the prior art. The pressing die comprises a bottom die, a core rod, impeller plug sheets, a material barrel, an upper die and the like. The pressing method comprises the following steps that a feeding tray is selected, vinylidene fluoride granules are added into the feeding tray for heating, the die is processedand assembled, the impeller movable ring sleeves the core rod of the die, and by the steps of heating the die and adding materials and pressing and the like, the vinylidene fluoride impeller with themovable ring is produced. The pressing die is suitable for single-piece small-batch production, the die investment is small, the cost is low, and the problem that the vinylidene fluoride production cost is high is effectively solved. According to the pressing die, the impeller movable ring and the impeller are formed in one step, additional bonding is not needed, and the reliability of the movable ring is improved, the time is saved, and the productivity is improved. The pressing die has the advantages that the production method is simple, and the popularization of the vinylidene fluoride impeller is facilitated.
Owner:鸿浩泵业有限公司

A kind of immobilized cephalosporin C acylase and preparation method thereof

The invention discloses immobilized cephalosporin C acylase and a preparation method thereof. The immobilized cephalosporin C acylase comprises ES-1 resin and cephalosporin C acylase fixed on the ES-1 resin. The preparation method comprises steps as follows: the ES-1 resin and the cephalosporin C acylase are dissolved in a phosphate buffer solution with the concentration being 0.5-1.0 mol / L and the pH being 7.0-10.5 and immobilized at the temperature of 15-35 DEG C for 8-20 h. Compared with the prior art, the provided method for preparing the immobilized cephalosporin C acylase is short in immobilizing time and high in enzyme activity recovery rate, the problem of high production cost in the prior art is solved, and the method is more suitable for industrial production.
Owner:SHANGHAI INST OF PHARMA IND CO LTD +1

A kind of water-based ultra-thin fireproof and thermal insulation material and preparation method thereof

The invention discloses a water-borne ultrathin type fireproof heat insulating material and a preparation method thereof. The water-borne ultrathin type fireproof heat insulating material comprises the following components in percentage by weight: 20-40% of puffed basalt particles, 15-30% of water, 35-60% of composite resin, 1.5-3% of a stabilizer, 0.5-1.8% of a dispersant, 0.2-0.5% of a leveling agent, 0.2-0.5% of a thickening agent, 0.2-0.5% of a defoaming agent and 1.5-3% of a heat insulating material MAS. The water-borne ultrathin type fireproof heat insulating material disclosed by the invention is green and environment-friendly, does not release formaldehyde, benzene and other harmful gases, and is stable in performance and high in fireproof heat insulating quality. The water-borne ultrathin type fireproof heat insulating material is simple in preparation process and wide in application range, the problem of high manufacturing cost of EPS, XPS and cement foamed heat insulating plates is solved, meanwhile the working strength and stress of constructors are relieved, the construction difficulty during measuring of special shaped structural parts, cutting of plates and the like by the constructers is reduced, and the construction cost and constriction difficulty are reduced at the same time.
Owner:赵丽芳

A kind of preparation method of molecular sieve and its application and utilization method of waste water

The invention discloses a preparation method of a molecular sieve, its application and a waste water utilization method. The method comprises the following steps: (1) uniformly mixing raw materials in water to obtain a mixture; (2) placing the mixture in a hydrothermal reaction kettle for crystallization, and after the crystallization is completed, solid-liquid separation to obtain solid products and mother liquor wastewater, Collecting the mother liquor wastewater; (3) washing, drying and calcining the solid product to obtain a molecular sieve; or adding the solid product to an ion exchange liquid containing copper ions for ion exchange, and then performing solid-liquid separation, washing, drying and calcining in sequence Molecular sieves are obtained after treatment; (4) repeating step (1), while recycling the mother liquor wastewater collected in step (2) and adding it to step (1), so that the raw material in step (1) comes from the mother liquor wastewater. The invention solves the technical problems of serious energy consumption and high production cost in wastewater treatment.
Owner:HUAZHONG UNIV OF SCI & TECH

Vertical cavity surface emitting laser based on van der Waals epitaxy and its fabrication method

A vertical cavity surface emitting laser based on van der Waals epitaxy and its manufacturing method. In the above manufacturing method, making a GaN-based active region structure includes: covering a single layer of two-dimensional material on a first substrate; The GaN-based active region structure is epitaxially grown on the first substrate of the dimensional material. The GaN-based active region structure includes from bottom to top: an n-type GaN layer, an active region, an electron blocking layer, a p-type GaN layer and a p+GaN layer, and the p+GaN layer serves as the first ohmic contact layer. A first DBR structure is formed on the second substrate. The GaN-based active region structure is peeled off from the first substrate. The stripped GaN-based active region structure is bonded to the first distributed Bragg reflector structure on the second substrate. A second DBR structure is formed over the first ohmic contact layer of the bonded structure. A vertical-cavity surface-emitting laser with low threshold current density and high gain is formed.
Owner:TSINGHUA UNIV

Method for producing baohuoside I through herba epimdii

The invention discloses a method for producing baohuoside I through herba epimdii. The method comprises the following steps of: S1. expanding propagating and diluting saccharomyces cerevisiae in culture medium; S2. mixing and evenly stirring culture solution and herba epimdii, and carrying out close and static anaerobic fermentation in a fermentation tank; S3. when the herba epimdii is entirely hydrolyzed, extracting fermenting raw materials with an extraction solvent; S4. washing with extract dichloromethane, and extracting ethyl acetate; and S5. filtering ethyl acetate through an alumina column, then carrying out silica gel adsorption column chromatography to obtain pure baohuoside I. The method has the beneficial effects of being simple and convenient to operate, easy to implement, high in efficiency, fast, low in cost, high in yield, pollution-free by adopting microbial solid-state anaerobic fermentation to hydrolyze herba epimdii-containing raw materials, thus being suitable for large-scale industrial production.
Owner:XICHANG DANYANG BIOLOGY TECH CO LTD

A gene encoding β-1,4-glucanase and its preparation and application

The invention discloses a preparation method and application of internally tangent beta-1,4-glucanase gene derived from paenibacilluspolymyxa and its enzyme. The preparation method is a technique method utilizing genetic engineering. The internally tangent beta-1,4-glucanase gene is cloned to an escherichia coli expression vector to obtain an escherichia coli recombination strain which can heterologously express the enzyme, and the strain heterologously expresses the prepared internally tangent beta-1,4-glucanase and can efficiently degrade konjac glucomannan (also known as konjac glucomanna).The prepared internally tangent beta-1,4-glucanase can be widely applied the fields of preparation of agriculture, foods, feed additives, medicine, oligo-glucomannan and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Method for culturing schizochytrium limacinum by utilizing vinasse treating fluid

The invention discloses a method for culturing schizochytrium limacinum by using a vinasse treating fluid, and belongs to the field of microbial fermentation production. According to the method for culturing schizochytrium limacinum by using the vinasse treating fluid, fermentation production is performed by taking the vinasse treating fluid as a main nitrogen source, dry schizochytrium limacinum powder is prepared by soaking, boiling, filtering and centrifuging vinasse, preparing a fermentation culture medium, setting fermentation conditions, collecting and drying schizochytrium limacinum, and algae oil is extracted. The method is a brand new process for fermentation production of schizochytrium limacinum by taking the vinasse treating fluid as a main nitrogen source, and the vinasse treating fluid is used for replacing high-price tryptone and yeast powder in a traditional culture medium, so that the cost of fermentation production is reduced, growth of schizochytrium limacinum and accumulation of DHA are promoted, and the yield of the schizochytrium limacinum is increased. Meanwhile, the environmental problem caused by stacking of the waste vinasse in the liquor factory is effectively solved; the problem of high production cost in the prior art is well solved, and high-value utilization of the vinasse is realized.
Owner:JIANGNAN UNIV

A gene encoding endo-β-1,4-mannanase and its preparation and application

The invention discloses an endo-β-1,4-mannanase gene derived from Paenibacillus polymyxa and the preparation method and application of the enzyme. Cloning the gene of cutting β‑1,4‑mannanase into the expression vector of Escherichia coli to obtain a recombinant strain of Escherichia coli that can express the enzyme heterologously Glycanase can efficiently degrade konjac polysaccharides (also known as konjac glucomannan). The endo-β-1,4-mannanase provided by the invention can be widely used in the fields of agriculture, food, feed additive, medicine, glucomannan oligosaccharide preparation and the like.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

A kind of breeding method of green pagoda cauliflower hybrid

The invention discloses a method for breeding a green pagoda cauliflower hybrid. In the method, the hybrid progeny of pagoda cauliflower and green cauliflower is obtained through microspore culture or a DH line or a self-bred pure line obtained through traditional breeding. As the male parent, the ogura cytoplasmic male sterile line of common pagoda cauliflower obtained by multi-generation backcrossing is used as the female parent, and a new generation of green pagoda cauliflower hybrid is obtained through crossing. The hybrid has excellent comprehensive resistance, is more cold-tolerant, has green curds, and has better ornamental value.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Breeding method of green pagoda cauliflower hybrid

The invention discloses a breeding method of a green pagoda cauliflower hybrid. A green pagoda cauliflower first-filial generation new variety is obtained by subjecting a pagoda cauliflower and greencauliflower hybrid filial generation to microspore culture or taking conventional breeding selfing homozygous DH line or selfing pure line as a male parent and common pagoda cauliflower ogura cytoplasm male sterile line obtained by multi-generation backcross breeding as a female parent by means of hybridization. The hybrid is excellent in comprehensive resistance, resistant to cold and green in flower ball, thereby having high ornamental value.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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