A kind of breeding method of green pagoda cauliflower hybrid
A pagoda flower and hybrid technology, which is applied in the field of selection and breeding of green pagoda cauliflower hybrids, can solve the problems of difficulty in available resources, lack of pagoda cauliflower germplasm resources, etc.
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Embodiment 1
[0024] Such as figure 1 Shown, present embodiment provides a kind of breeding method of green pagoda cauliflower hybrid, specifically comprises the following steps:
[0025] 1) Screening of germplasm resources. Since 2010, 7 pagoda-type cauliflower germplasm resources and 2 green cauliflower germplasm resources have been collected. Through the observation and identification of agronomic characters, 1 ogura male sterile pagoda-type cauliflower and 3 fertile pagoda-type cauliflowers, as well as 2 usable green cauliflower germplasms with different ripening stages were screened out.
[0026] 2) Male sterility source selection. The ogura male sterile pagoda-type cauliflower hybrid, denoted as bj1841, showed normal petals and nectary, and it was used as a male sterile source breeding material.
[0027] 3) Selection and breeding of recurrent parents. Using fertile pagoda-type broccoli hybrids as donors, denoted as RCK01, 13 strains of double haploid series were obtained through m...
Embodiment 2
[0031] Embodiment 2 parent breeding
[0032] Because pagoda cauliflower needs to be cut many times and cultivated over winter, it has a strong winter nature, so parents need to be bred in a greenhouse to prevent the wounds after cutting from being infected by rainwater. Because of the self-compatibility of the recurrent parent, seeds can be obtained through assisted pollination by releasing bees in a separate shed; the male sterile line is planted in a separate shed with a ratio of 1:1 to the male sterile line and the recurrent parent, which needs to be pollinated by releasing bees. Seeds are obtained by backcrossing; the male parent is a self-incompatible DH line, which needs to be pollinated manually at the flowering stage in a greenhouse to obtain seeds.
Embodiment 3
[0033] Embodiment 3 hybrid seed production
[0034] If a large amount of seed production is required for the first-generation hybrid species, it is possible to find a suitable area for isolated seed collection in open-air sun borders, and under normal circumstances, isolated seed production can be carried out in a greenhouse.
[0035] (1) Choose a planting greenhouse: According to the production area, choose an ordinary plastic greenhouse, glass greenhouse or solar greenhouse. It is best not to plant cruciferous crops in the previous work, or the greenhouse soil has been sterilized. Apply base fertilizer and borax before transplanting.
[0036] (2) Ratio of sowing and planting: the parents raise seedlings and transplant, and plant according to the ratio of female parent: male parent 2:1 or 3:2. Because the growth periods of the female parent and the male parent are slightly different, and because the growth periods of multiple male parents are different, it is necessary to st...
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