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37results about How to "Suitable for large-scale operations" patented technology

Polybasic layered oxide lithium ion battery material and preparation method thereof

The invention belongs to the technical field of lithium ion battery materials, and particularly relates to a polybasic layered oxide lithium ion battery material and a preparation method thereof. The battery material is shown as Li(Ni1-x-y-zCoxMnyMz)O2, wherein M is Zn, Mg, Al, Cu, As, Cd or Pd; and each metal element in a single particle of the material is distributed in a graded way at different thickness distances away from the center of the particle. The polybasic layered oxide lithium ion battery material which is easy to regulate and control is prepared successfully by adopting the simple and practicable method. As proved by an electrochemical test result, the polybasic layered oxide lithium ion battery material provided by the invention has high charging and discharging reversibility and high cycle performance.
Owner:ZHENGZHOU UNIV

Immobilized recombinant penicillin G acylase and application thereof

The invention discloses an immobilized recombinant penicillin G acylase and an application thereof to preparation of (S)-2-aryl-amino acid and cephalosporin antibiotics. The immobilized recombinant penicillin G acylase disclosed by the invention is capable of converting an S-type substrate into an S-type product within shorter time, high in substrate tolerance and strict in S selectivity for the substrate and structural analogues thereof; due to the addition of cobalt ions as well as a protective agent with phenylacetic acid and glycerin as enzyme active centers, the immobilized recombinant penicillin G acylase disclosed by the invention is prevented from being successfully subjected to multipoint covalent immobilization on the surface of an epoxy resin carrier under the condition that enzyme molecules are seriously inactivated in an immobilization process, and the immobilization method is simple and feasible, cheap in raw materials and suitable for large-scale operation; the immobilized recombinant penicillin G acylase can be used for synthesizing cephalosporin antibiotics and splitting the prepared (S)-2-aryl-amino acid, and can be continuously used for more than 50 batches before the activity of the immobilized enzyme is not remarkably reduced.
Owner:ZHEJIANG UNIV OF TECH

Method of constructing selection-marker-free self-luminescent mycobacterium abscessus and establishment of corresponding in-vitro high-flux medicine-screening model

The invention discloses a method of constructing selection-marker-free self-luminescent mycobacterium abscessus and establishment of a corresponding in-vitro high-flux medicine-screening model. During the construction process, a key factor is the construction of a recombinant plasmid pOPAI1B, which contains a fused gene including a luminescent required enzyme gene LuxCDABE, a resistance screening gene Apr and an integrase gene Int; direct repeat sequences DifR and DifL at the two ends of the fused gene respectively; and a phage integration site attP and a replicon. The recombinant plasmid pOPAI1B is transformed into the mycobacterium abscessus and then is screened to obtain the selection-marker-free self-luminescent mycobacterium abscessus of which a resistance gene and the integrase gene are both lost. The self-luminescent mycobacterium abscessus can screen drugs in a high flux, can determine sterilizing or bacteria-resistant effects of the drug by means of detection of luminescent situation of bacteria, can be used for in-vitro drug activity detection, is free of any substrates, can continuously detect the same sample, is high in sensitivity and is strong in repeatability.
Owner:GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI +2

Renaturation method of COVID-19 recombinant protein inclusion bodies

The invention discloses a renaturation method of COVID-19 recombinant protein inclusion bodies. According to the renaturation method of the COVID-19 recombinant protein inclusion bodies provided by the invention, after a denatured inclusion body dissolution product is treated by using a nickel chelating column, a renaturation solution containing a denaturant and a reduced concentration gradient ofthe denaturant is used for gradient elution at normal temperature for flow renaturation, so that the method not only reduces the costs, but also significantly shortens the time required for the renaturation of the COVID-19 recombinant protein inclusion bodies, thereby facilitating large-scale renaturation of the COVID-19 recombinant protein inclusion bodies in a short time and at a lower cost; and the protein recovery is high, the purity is high, and the stability is strong, so that a large number of high-quality COVID-19 proteins can be conveniently obtained in a short time and at a lower cost for COVID-19 detection and antibody preparation. The renaturation method of the COVID-19 recombinant protein inclusion bodies provided by the invention is suitable for the renaturation of the COVID-19 RBD recombinant protein inclusion body or the COVID-19 N recombinant protein inclusion body.
Owner:艾柏森(江苏)生物科技有限公司

Bright hard wear-resistant inked rope tip and production process thereof

ActiveCN102851986ASimple operation processSimple production process implementationBinding endsFibre treatmentWear resistantEngineering
The invention discloses a bright hard wear-resistant inked rope tip. The inked rope tip is produced by the following method: a rope tip is soaked in setting size and solidified; the rope tip is soaked in ink, solidified and colored; finally, the rope tip is soaked in glossy varnish and solidified, and thereby the bright hard wear-resistant inked rope tip is obtained. The bright hard wear-resistant inked rope tip produced by the invention not only is as neat as ordinary rope tips made of plastic and metal, but also has the advantages that: the bright hard wear-resistant inked rope tip is highly glossy, the lines of a rope can be shown, and colors can be optionally matched.
Owner:广东康派环创科技有限公司

Incomplete antibody screening colloidal gold reagent kit and preparation method thereof

The invention discloses an incomplete antibody screening colloidal gold reagent kit and a preparation method thereof. The reagent kit comprises a first erythrocyte membrane antigen gold particle solution, a second erythrocyte membrane antigen gold particle solution, a third erythrocyte membrane antigen gold particle solution and an antihuman globulin solution, wherein an antigen spectrum of each of the first erythrocyte membrane antigen gold particle solution, the second erythrocyte membrane antigen gold particle solution and the third erythrocyte membrane antigen gold particle solution collectively include D, C, E, c, e, Jka, JKb, M, N, S, s, Fya, Fyb, Dia, K, k, Lea, Leb and P1 antigens. The reagent kit is long in storage time and easy to operate, the complicated process such as centrifuging, washing, and incubation is not needed, and a result is clear and easy to judge. The reagent kit also has the advantage of being suitable for large-scale operation and standard operation.
Owner:INTEC PROD INC

Method and kit for quickly building plasma DNA sequencing library

The invention provides a method for quickly building a plasma DNA sequencing library. The method includes the following steps: 1), extracting plasma DNA; 2), performing end filling on the plasma DNA to obtain plasma DNA subjected to end filling; 3), performing 3' suspension A on the plasma DNA subjected to end filling to obtain plasma DNA after 3' suspension A; 4), connecting the plasma DNA after 3' suspension A with a sequencing joint to obtain a connection product; 5), purifying the connection product to obtain a purified product. The invention further provides a kit for building the plasma DNA sequencing library. Building process of the plasma DNA sequencing library is simplified greatly, pollution of environment and pollution among samples due to PCR amplification is removed, requirements, on environment, of building of the plasma DNA library are lowered, nonuniformity in genome covering caused by the process of PCR is avoided, building of the plasma sample library is enabled to be lower in cost, higher in efficiency and quick, and plasma DNA sequencing results are truer and more effective.
Owner:北京贝瑞和康医学检验实验室有限公司

Autonomously illuminated Acinetobacter baumannii and construction method and application thereof

The invention discloses a transfer plasmid for transforming Acinetobacter baumannii, which comprises, in clockwise order, a replicon ori, an ampicillin resistance gene AmpR, a transposon sequence, a junction transfer initiation site oriT, the transposon sequence contains a base sequence capable of autonomously emitting Acinetobacter baumannii and a resistance gene, and both ends of the resistancegene are provided with DifR and DifL sequences; also disclosed is an autonomously illuminated Acinetobacter baumannii without a resistance marker, the gene of Acinetobacter baumannii containing a genecapable of expressing autonomous light-emitting protein, and no resistance screening gene is in the genome of Acinetobacter baumannii. In the present invention, the autonomously illuminated Acinetobacter baumannii, which is constructed by the transfer plasmid pUC18T-mini-Tn7T-lux-Ab-dif-Apr and the auxiliary plasmid pTNS3, can emit light without adding any substrate; and luminescent colonies canbe seen with the naked eye in a dark environment.
Owner:GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI

High-branch grafting method for jinlinghong

InactiveCN108651034AImprove water retention capacityReduce water lossGraftingFertilizerBud
The invention relates to a high-branch grafting method for jinlinghong. The method comprises the specific steps that scions are collected from the middle and upper parts of developmental branches of 1-2 years old which contain 1 or 2 full buds; jinlinghong seedlings of 2-3 years old are selected, anvil cutters are conducted at 30 cm, and the seedlings are used as rootstock; a cutting slip preservation method is to place the scions into paraffin wax for waxing, and the entire scion is wrapped with the wax; a tree is shaped and pruned; the rootstock and the smooth portion of bark near the groundare cut, one side of the rootstock section is cut into long slots, the scions are inserted into the rootstock, and 1-2 buds of the scions are exposed to outside; 3-4 weeks after grafting, the survival condition is checked, and loosening is conducted after one month; water spraying management is conducted on grafted plants; the plants survived are sprayed with a fungicide and an insecticide once amonth; after leaves fall in autumn, the fallen leaves are removed, and a base fertilizer is applied; the invention discloses the high-branch grafting method for the jinlinghong, and the method can improve the water retention of the post-harvest scions, be beneficial to the handling and maintaining of the scions, and improve grafting efficiency.
Owner:NANJING DENGBO BIOTECH

Method for recycling calcium fluoride from dolomite type magnetite magnetically-separated tailings

The invention discloses a method for recycling calcium fluoride from dolomite type magnetite magnetically-separated tailings. The method comprises the steps of 1, grinding work; 2, flotation work, wherein one time of coarse flotation, one time of sweeping and five times of refined flotation are performed; 3, calcination work; 4, acid leaching work. Compared with fluorite and dolomite obtained through traditional and simple separating flotation, better efficiency is achieved, the fluorite grade can reach 90% or above, the recycling rate is larger than 80%, the dolomite grade can reach 90% or above, the recycling rate is larger than 80%, and meanwhile a reliable scheme is provided for flotation separation of fluorite and dolomite minerals.
Owner:WUHAN UNIV OF SCI & TECH

Technique for detection by using polybrene microplate before blood transfusion

InactiveCN101644712ASuitable for large-scale operationsSuitable for standardized operationsBiological testingAgglutinationPolymer
The invention relates to a technique for detection by using an improved polybrene microplate before blood transfusion. In the technique, the designed microplate (figure 1) is used instead of used consumable items and supporting facility and no test tube nor centrifuge is used, and on the other hand, the concentration and dosage of a polybrene reagent are changed. The polybrene is a tetraamine polymer which can neutralize negative electric charges on red blood cells, reduce the Zeta electric potential and shorten the distance between cells. Under the condition of a low concentration, a reversible nonspecific hemagglutination phenomenon can appear when the distance between the red blood cells is shortened; and when the concentration of the polybrene is increased, the red blood cells directlyform nonspecific hemagglutination. When the negative electric charges on the surfaces of the red blood cells are restored, the hemagglutination phenomenon disappears because of the repulsion of the negative electric charges among the red blood cells, but the affinity or the agglutination, really caused by the antigen antibodies of the red blood cells, of the antigen antibodies does not disappear.
Owner:刘景春 +2

Spherical lithium ion battery anode material and preparation method of precursor thereof

ActiveCN102760875AGood charge and discharge reversibilityPromote circulationCell electrodesElectrical batteryPhysical chemistry
The invention relates to a spherical lithium ion battery anode material and a preparation method of a precursor thereof. The preparation method comprises the following steps of: with newly-prepared nano flaky nickel hydroxide as a raw material, dispersing the raw material in an aqueous solution, adding a certain proportion of cobalt salt into the aqueous solution, reacting for several hours in an enclosed vessel to obtain uniform spherical granules which can be used as a precursor for preparing the lithium ion battery anode material; and uniformly mixing the obtained precursor granules and a lithium salt and carrying out high-temperature calcining to finally obtain the spherical lithium ion battery anode material with uniform granule size. The preparation method disclosed by the invention is simple to operate, high in efficiency, easy in obtainment of raw material, low in cost and environment-friendly; and the prepared spherical lithium battery anode material has good electrochemical property.
Owner:ZHENGZHOU UNIV

Application of ascorbic acid as sleep release accelerating agent in breaking seed dormancy

The invention discloses an application of ascorbic acid as a sleep release accelerating agent in breaking seed dormancy. According to the invention, a fact that ascorbic acid can break seed dormancy and can promote germination and the build of normal seedling form is found for the first time. Through the utilization of the method provided by the invention, the effect of breaking dormancy is the same as the effect of gibberellin, but the cost is low and no pollution is generated; compared with low-temperature stratification treatment, the method has greatly shortened time for breaking the seeddormancy, and the operation is convenient; and the guarantee of the emergence rate of seeds in conventional seedling breeding is facilitated. The method provided by the invention is simple and convenient to operate, is economical, practical and environmentally friendly, does not need special equipment, can be operated on a large scale, and can be demonstrated, promoted and applied to cultivation practice.
Owner:SHAANXI NORMAL UNIV

Integrative plasmid pOPHI and resistance screening marker-free self-luminescent mycobacterium

The invention discloses an integrative plasmid pOPHI and a resistance screening marker-free self-luminescent mycobacterium. The integrative plasmid pOPHI comprises a promoter, an enzyme gene (LuxCDABE) needed for luminescence, a fusion gene of an integrase gene (Int) and a resistance screening gene, direct repeat sequences DifR and DifL positioned on two ends of the fusion gene, a phage integration site (attP) and a replicon. After the integrative plasmid pOPHI is transferred into the mycobacteria, a photobacterium of which the resistance gene and the integrase gene are lost is screened out by subculturing, and the photobacterium is the resistance screening marker-free self-luminescent mycobacterium. Whether the resistance screening marker-free self-luminescent mycobacterium obtained by construction is dead or not is judged according to whether the bacterium is luminescent or not, the mycobacterium can be used for a plurality of detections of experiments, and credible experimental data can be quickly obtained.
Owner:GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI

Fireproof heat-isolating building material and preparation method thereof

The invention provides a fireproof heat-isolating building material and a preparation method thereof and relates to the field of building materials. The building material is prepared from the following raw materials in parts by weight: 15-30 parts of stearyl alcohol, 8-15 parts of sepiolite powder, 5-10 parts of attapulgite powder, 20-25 parts of glass beads, 5-10 parts of bamboo carbon powder, 20-25 parts of gypsum boards, 50-70 parts of calcium carbonate powder, 5-10 parts of foamed ceramic plates, 15-20 parts of glass wool, 4-6 parts of fibroin fibers and 15-20 parts of 1,4-butenediol. The preparation method of the building material comprises the following steps of: crushing; stirring; pouring; and cooling. The building material provided by the invention solves the problem that an existing fireproof heat-isolating building material is poor in fireproof heat-isolating effect and high in cost.
Owner:肥西县碧涛建材有限公司

Paper protection method for improving surface hydrophobicity

The invention provides a paper protection method for improving surface hydrophobicity. The paper protection method comprises the following steps: 1) soaking paper with an alkaline solution; 2) applying a zinc acetate dehydrate solution to the paper, and reacting on the surface of the paper to generate a zinc oxide precursor; and 3) carrying out dehydration reaction on the treated paper at 70-85 DEG C to obtain the paper with flaky zinc oxide on the surface. According to the method, sheet-shaped zinc oxide crystals in a specific form are loaded on paper fibers to improve the hydrophobicity of the surface of the paper, so that the hydrophobicity of the surface of the paper can be improved under the condition that ink marks, pigments and paper bodies on the surface of the paper are not damaged.
Owner:HANGZHOU ZHONGCAI TECH CO LTD

Drying device

The invention provides a drying device. The drying device comprises a main body; an end cover is arranged at the upper end; a drying fixed frame is arranged in the main body; a temperature monitor and a humidity monitor are arranged in the main body, and are both electrically connected with a control box; the control box is arranged at the external of the main body; and an air replenishing machine and a vacuum pump are arranged out of the main body, and are both electrically connected with the control box. The drying device can keep dry environment through automatic control of temperature and humidity, and facilitates long-time storage.
Owner:天津龙川鑫汇实验设备制造有限公司

A reciprocating kiwi fruit slicer

The invention discloses a reciprocating kiwifruit slicing device which is characterized by comprising a first cutter assembly and a second cutter assembly; upper sliding strip bulges are embedded into clearances among adjacent lower sliding strip bulges; the lower sliding strip bulges are embedded into clearances among the adjacent upper sliding strip bulges; the cutting edges of a first group of blades are in flush with those of a second group of blades; a left upper sliding strip and a left lower sliding strip are positioned in a left sliding groove; a right upper sliding strip and a right lower sliding strip are positioned in a right sliding groove; the upper sliding strip bulges are in clearance fit with the adjacent lower sliding strip bulges; the upper sliding strip bulges and the adjacent lower sliding strip bulges can oppositely slide along the direction of the cutting edges; guide holes being opposite to all threaded holes are formed in the outer side walls of the left and right sliding grooves; and guide columns connected with the threaded holes penetrate through the guide holes. By adoption of the reciprocating kiwifruit slicing device, the kiwifruit can be cut into kiwifruit slices being uniform in thickness, and the possibility of kiwifruit deformation can be lowered.
Owner:颍上县城乡产业建设有限公司

Dig-pull type cassava harvester

The invention, which belongs to the field of agricultural machines, relates to a dig-pull type cassava harvester, which comprises a chassis (1), the front part of the chassis is provided with a suspension (4), the lower part of the chassis is connected with a digger blade (2), a clamping conveyer (5), the front end of which is low and the rear end of which is high, is arranged on the chassis, andthe clamping conveyer is connected with a power source of a tractor through a transmission. The cassava harvester can utilize the digger blade and the clamping conveyer to dig and harvest cassavas atthe same time, and thereby is characterized by labor saving, easy harvesting, high efficiency, high speed, high automation degree and large-scale operation.
Owner:HAINAN UNIVERSITY

Cleaning method, special cleaning agent and special cleaning equipment for color steel tile with cement ash clinker ash attached to surface

The invention belongs to the technical field of cement ash color steel tile cleaning, and particularly relates to a cleaning method, a special cleaning agent and special cleaning equipment for a color steel tile with cement ash clinker ash attached to the surface. The special cleaning agent comprises the following components in parts by mass: 23.3-23.7 parts of adipic acid, 12.6-12.9 parts of tartaric acid, 10.6-11 parts of oxalic acid, 17-19 parts of hydrochloric acid with the content being 33%, 5.2-5.5 parts of hydrofluoric acid, 65.5-68.5 parts of artemisia argyi and 38-40 parts of purified water. The special cleaning equipment comprises a machine tool base, an ultrasonic generation device, an atomization plate, a distilled water storage tank, a cleaning agent storage tank, a control system, a testing device and an electrochemical regulation and control device. According to the cleaning method, a cement ash clinker ash attachment layer attached to the surface of the color steel tile can be perfectly removed, and meanwhile a paint layer on the surface of the color steel tile cannot be damaged. The color steel tile cleaning method has the technical advantages of being high in efficiency, good in effect, suitable for large-scale operation and free of damage to a color steel tile body.
Owner:昆明环节科技有限公司

A kind of incomplete antibody screening colloidal gold kit and preparation method thereof

The invention discloses an incomplete antibody screening colloidal gold reagent kit and a preparation method thereof. The reagent kit comprises a first erythrocyte membrane antigen gold particle solution, a second erythrocyte membrane antigen gold particle solution, a third erythrocyte membrane antigen gold particle solution and an antihuman globulin solution, wherein an antigen spectrum of each of the first erythrocyte membrane antigen gold particle solution, the second erythrocyte membrane antigen gold particle solution and the third erythrocyte membrane antigen gold particle solution collectively include D, C, E, c, e, Jka, JKb, M, N, S, s, Fya, Fyb, Dia, K, k, Lea, Leb and P1 antigens. The reagent kit is long in storage time and easy to operate, the complicated process such as centrifuging, washing, and incubation is not needed, and a result is clear and easy to judge. The reagent kit also has the advantage of being suitable for large-scale operation and standard operation.
Owner:INTEC PROD INC

Cement cleaning agent and preparation and application methods thereof

PendingCN114317129ANo corrosive damageDoes not cause chromatic aberrationInorganic/elemental detergent compounding agentsCationic surface-active compoundsActive agentEngineering
The invention discloses a cement cleaning agent as well as preparation and application methods thereof. The cement cleaning agent consists of a solution A, a solution B and a solution C, the solution A is prepared from the following components in percentage by mass: 16 to 32 parts of nano magnesium hydroxide, 15 to 26 parts of cationic surface active agent and 942 to 969 parts of water; the solution B is prepared from 240 to 320 parts of organic acid, 180 to 260 parts of inorganic acid, 98 to 127 parts of dispersing agent, 8 to 14 parts of complexing agent and 279 to 474 parts of water; the solution C is prepared from 980 to 988 parts of water and 12 to 20 parts of urea. The cement cleaning agent can penetrate into hardened cement to efficiently remove thick cement residues adhered to the surface of a material, and meanwhile, the material is not damaged. The prepared cement cleaning agent is high in efficiency, good in effect, suitable for large-scale operation and good in adaptability, and has good social benefits and economic benefits.
Owner:云南森博混凝土外加剂有限公司

Flue gas dust removal system

The invention discloses a flue gas dust removal system, which comprises a cooling well and a flue gas conveying pipe; the cooling well is located above a foundation and extends upwards, wherein the upper end of the cooling well is provided with a transition chamber in a communicating mode, the flue gas conveying pipe penetrates through the transition chamber and extends into the cooling well, andan observation window is arranged on the outer side face of the transition chamber; a smoke washing box is fixedly arranged at the upper end of the transition chamber and comprises a curved flow channel and a liquid collecting tank, and two sets of air guide pipes are symmetrically arranged at the upper end of the transition chamber and extend upwards to the two sides of the top of the smoke washing box. According to the flue gas dust removal system, by designing the cooling well, compared with traditional cooling structures such as a coal economizer and a water cooler, the cooling well utilizes underground water, so that the use cost is low, the contact time is greatly prolonged, the cooling effect on high-temperature flue gas is better, dust particles in the flue gas can be directly absorbed, and certain self-purification capacity is achieved. The system is suitable for large-scale operation, is suitable for different working conditions, and shows a better application prospect.
Owner:ANHUI GUONENG YISHENG ENVIRONMENTAL PROTECTION SCI & TECH

Reciprocating Chinese gooseberry slitting device

The invention discloses a reciprocating Chinese gooseberry slitting device, which is characterized by comprising a first knife assembly, a second knife assembly, a third knife assembly and a fourth knife assembly; upper draw slip protrusions fall into gaps between adjacent lower draw slip protrusions, the lower draw slip protrusions fall into gaps between the adjacent upper draw slip protrusions, the cutting edges of a first blade set and a second blade set are flush, a left upper draw slip and a left lower draw slip are arranged in a left chute, a right upper draw slip and a right lower draw slip are arranged in a right chute, the upper draw slip protrusions and the adjacent lower draw slip protrusions are in clearance fit and can slide oppositely along the cutting edges, guide holes opposite to threaded holes are formed in the outer walls of the left and right chutes, and guide posts connected with the threaded holes penetrates through the guide holes. By adopting the device, the cut Chinese gooseberry slits are uniform in thickness, and the deformation probability of Chinese gooseberry can be reduced.
Owner:重庆市麦腾农业开发有限公司

A reciprocating kiwi fruit cutting device

The invention discloses a reciprocating Chinese gooseberry slitting device, which is characterized by comprising a first knife assembly, a second knife assembly, a third knife assembly and a fourth knife assembly; upper draw slip protrusions fall into gaps between adjacent lower draw slip protrusions, the lower draw slip protrusions fall into gaps between the adjacent upper draw slip protrusions, the cutting edges of a first blade set and a second blade set are flush, a left upper draw slip and a left lower draw slip are arranged in a left chute, a right upper draw slip and a right lower draw slip are arranged in a right chute, the upper draw slip protrusions and the adjacent lower draw slip protrusions are in clearance fit and can slide oppositely along the cutting edges, guide holes opposite to threaded holes are formed in the outer walls of the left and right chutes, and guide posts connected with the threaded holes penetrates through the guide holes. By adopting the device, the cut Chinese gooseberry slits are uniform in thickness, and the deformation probability of Chinese gooseberry can be reduced.
Owner:重庆市麦腾农业开发有限公司

Pharmaceutical composition targeting kuptake cells for treating cancer

The invention provides a pharmaceutical composition for treating cancer, especially cancer liver metastasis, the pharmaceutical composition comprises Escherichia coli and a pharmaceutically acceptable excipient, the Escherichia coli comprises a recombinant vector, and the recombinant vector is connected with a nucleotide sequence of sgRNA of a target gene and Cas9 protein / Casphi protein. A CRISPR / Cas gene editing system coded by plasmid DNA is delivered into Kuptake cells through the recombinant vector, and in-situ gene editing and modification can be carried out on the Kuptake cells. By knocking out c-Maf and MafB gene expressions of Kuphog cells through the method, the lethality of Kuphog cells to metastatic liver tumors can be remarkably enhanced, and the purpose of treating the liver metastatic cancer is achieved.
Owner:UNIV OF SCI & TECH OF CHINA

Application of Ascorbic Acid as Dormancy-Relief Accelerator in Breaking Seed Dormancy

The invention discloses an application of ascorbic acid as a sleep release accelerating agent in breaking seed dormancy. According to the invention, a fact that ascorbic acid can break seed dormancy and can promote germination and the build of normal seedling form is found for the first time. Through the utilization of the method provided by the invention, the effect of breaking dormancy is the same as the effect of gibberellin, but the cost is low and no pollution is generated; compared with low-temperature stratification treatment, the method has greatly shortened time for breaking the seeddormancy, and the operation is convenient; and the guarantee of the emergence rate of seeds in conventional seedling breeding is facilitated. The method provided by the invention is simple and convenient to operate, is economical, practical and environmentally friendly, does not need special equipment, can be operated on a large scale, and can be demonstrated, promoted and applied to cultivation practice.
Owner:SHAANXI NORMAL UNIV

Blue algae processing system and processing method for ship

The present invention discloses a marine blue algae processing system and a processing method thereof. The system comprises a blue algae collector. The outlet end of the blue algae is orderly connected in series with a first grade stirring kettle, a gravitational separation machine, a second grade stirring kettle, a squeeze filter and a filter cake pool via pipes and valves. A three-grade processing method comprising primary condensation, secondary condensation and squeezing is adopted. The system is arranged on a running powerboat planarly or spatially according to the space of the boat. Thesystem uses the running speed of the boat to collect blue algae. The operating efficiency of processing blue algae can be quickly improved. Nuisance-free and reusable algae biscuits are formed. The operation is simple and convenient. The process flow system is highly effective and successive, and is suitable for large-scale operation.
Owner:JIANGSU UNIV
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