37results about How to "Suitable for large-scale operations" patented technology

The invention discloses an integrative plasmid pOPHI and a resistance screening marker-free self-luminescent mycobacterium. The integrative plasmid pOPHI comprises a promoter, an enzyme gene (LuxCDABE) needed for luminescence, a fusion gene of an integrase gene (Int) and a resistance screening gene, direct repeat sequences DifR and DifL positioned on two ends of the fusion gene, a phage integration site (attP) and a replicon. After the integrative plasmid pOPHI is transferred into the mycobacteria, a photobacterium of which the resistance gene and the integrase gene are lost is screened out by subculturing, and the photobacterium is the resistance screening marker-free self-luminescent mycobacterium. Whether the resistance screening marker-free self-luminescent mycobacterium obtained by construction is dead or not is judged according to whether the bacterium is luminescent or not, the mycobacterium can be used for a plurality of detections of experiments, and credible experimental data can be quickly obtained.

The invention discloses an immobilized recombinant penicillin G acylase and an application thereof to preparation of (S)-2-aryl-amino acid and cephalosporin antibiotics. The immobilized recombinant penicillin G acylase disclosed by the invention is capable of converting an S-type substrate into an S-type product within shorter time, high in substrate tolerance and strict in S selectivity for the substrate and structural analogues thereof; due to the addition of cobalt ions as well as a protective agent with phenylacetic acid and glycerin as enzyme active centers, the immobilized recombinant penicillin G acylase disclosed by the invention is prevented from being successfully subjected to multipoint covalent immobilization on the surface of an epoxy resin carrier under the condition that enzyme molecules are seriously inactivated in an immobilization process, and the immobilization method is simple and feasible, cheap in raw materials and suitable for large-scale operation; the immobilized recombinant penicillin G acylase can be used for synthesizing cephalosporin antibiotics and splitting the prepared (S)-2-aryl-amino acid, and can be continuously used for more than 50 batches before the activity of the immobilized enzyme is not remarkably reduced.

The invention relates to a spherical lithium ion battery anode material and a preparation method of a precursor thereof. The preparation method comprises the following steps of: with newly-prepared nano flaky nickel hydroxide as a raw material, dispersing the raw material in an aqueous solution, adding a certain proportion of cobalt salt into the aqueous solution, reacting for several hours in an enclosed vessel to obtain uniform spherical granules which can be used as a precursor for preparing the lithium ion battery anode material; and uniformly mixing the obtained precursor granules and a lithium salt and carrying out high-temperature calcining to finally obtain the spherical lithium ion battery anode material with uniform granule size. The preparation method disclosed by the invention is simple to operate, high in efficiency, easy in obtainment of raw material, low in cost and environment-friendly; and the prepared spherical lithium battery anode material has good electrochemical property.

The invention discloses a reciprocating Chinese gooseberry slitting device, which is characterized by comprising a first knife assembly, a second knife assembly, a third knife assembly and a fourth knife assembly; upper draw slip protrusions fall into gaps between adjacent lower draw slip protrusions, the lower draw slip protrusions fall into gaps between the adjacent upper draw slip protrusions, the cutting edges of a first blade set and a second blade set are flush, a left upper draw slip and a left lower draw slip are arranged in a left chute, a right upper draw slip and a right lower draw slip are arranged in a right chute, the upper draw slip protrusions and the adjacent lower draw slip protrusions are in clearance fit and can slide oppositely along the cutting edges, guide holes opposite to threaded holes are formed in the outer walls of the left and right chutes, and guide posts connected with the threaded holes penetrates through the guide holes. By adopting the device, the cut Chinese gooseberry slits are uniform in thickness, and the deformation probability of Chinese gooseberry can be reduced.

The invention discloses an application of ascorbic acid as a sleep release accelerating agent in breaking seed dormancy. According to the invention, a fact that ascorbic acid can break seed dormancy and can promote germination and the build of normal seedling form is found for the first time. Through the utilization of the method provided by the invention, the effect of breaking dormancy is the same as the effect of gibberellin, but the cost is low and no pollution is generated; compared with low-temperature stratification treatment, the method has greatly shortened time for breaking the seeddormancy, and the operation is convenient; and the guarantee of the emergence rate of seeds in conventional seedling breeding is facilitated. The method provided by the invention is simple and convenient to operate, is economical, practical and environmentally friendly, does not need special equipment, can be operated on a large scale, and can be demonstrated, promoted and applied to cultivation practice.