Application of ascorbic acid as sleep release accelerating agent in breaking seed dormancy
A technology of ascorbic acid and accelerator, which is applied in ascorbic acid as a dormancy-relieving accelerator, breaks the field of seed dormancy, and can solve the problems of seed germination rate, delay in production season, economic loss, etc., to achieve large-scale operation, improve utilization rate, The effect of low equipment requirements
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Embodiment 1
[0017] The seeds of Duli pear collected in Xinjiang in 2016 were rinsed with distilled water to remove impurities and then air-dried in a cool place. The seeds were stored in ziplock bags at room temperature. Get the stored Duli pear seeds and imbibition in distilled water for 8 hours, then use a mass fraction of 2% sodium hypochlorite aqueous solution for disinfection for 3 minutes, then rinse with distilled water for 3 to 5 times, and then wash the seeds with secondary distilled water (i.e. ascorbic acid concentration of 0, as a control group), 10mmol / L ascorbic acid aqueous solution, 25mmol / L ascorbic acid aqueous solution, 50mmol / L ascorbic acid aqueous solution, 75mmol / L ascorbic acid aqueous solution, 100mmol / L ascorbic acid aqueous solution soaked at normal temperature (22 ℃) for 3 hours, after treatment Blot the moisture from the surface of the seeds with sterile filter paper.
[0018] The above-mentioned treated seeds were placed in an environment of 16 hours of light...
Embodiment 2
[0029] In 2016, the Zhengzhou Comprehensive Experimental Station of the National Pear Industry System reached the physiological maturity stage. The ripe fruits of the early crisp red pears were harvested and immediately transported back to the laboratory. Store in a ziplock bag at room temperature. Take the stored Zaosu red pear seeds and absorb them in distilled water for 24 hours, carefully peel off the inner and outer seed coats of the seeds, do not damage the seed embryos, and then disinfect them with 2% sodium hypochlorite aqueous solution for 2 minutes, and then use distilled water After washing 3 to 5 times, the seeds were washed with twice distilled water (that is, ascorbic acid concentration is 0, as a control group), 1mmol / L ascorbic acid aqueous solution, 5mmol / L ascorbic acid aqueous solution, 10mmol / L ascorbic acid aqueous solution, 15mmol / L ascorbic acid aqueous solution, 20mmol / L ascorbic acid aqueous solution was soaked at normal temperature (22°C) for 3 hours,...
Embodiment 3
[0035]In 2015, after the fruits of Mahali cherries in Sanyuan County, Shaanxi Province matured and softened, the seeds were taken out, washed with distilled water to remove impurities, and then dried in a cool place. The seeds were stored in ziplock bags at room temperature. After taking the stored Zaosu red pear seeds and absorbing them in distilled water for 24 hours, carefully peel off the inner and outer seed coats of the seeds (cherry seeds have a harder outer seed coat, in order to ensure that the embryo can fully absorb the ascorbic acid solution, so in the Peel off the outer seed coat during the test), do not injure the seed embryo, then use a mass fraction of 2% sodium hypochlorite aqueous solution to disinfect for 3 minutes, then rinse with distilled water for 3 to 5 times, and then wash the seeds with secondary distilled water (i.e. The ascorbic acid concentration is 0, as the control group), 10mmol / L ascorbic acid aqueous solution, 30mmol / L ascorbic acid aqueous sol...
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