The invention discloses a natural Chinese herbal health care wine and a making method thereof. The natural Chinese herbal health care wine is prepared according to theories of traditional Chinese medicine and the efficacy generated by single use and compatibility of all raw materials. The carefully selected 33 Chinese herbal medicines polygonatum, radix asparagi, rhizoma atractylodis, pine needle, barbary wolfberry fruit, codonopsis pilosula, radix rehmanniae, poria cocos, bighead atractylodes rhizome, white paeony root, Angelica sinensis, medicated leaven, Ligusticum wallichii, sweet osmanthus, longan pulp, ginseng, rehmannia glutinosa, dwarf lilyturf tuber, radix asparagi, poria cocos, radix rehmanniae, agilawood, rose, rosa multiflora, plum blossom, peach blossom, leek flower, walnut flesh, Chinese wolfberry, Angelica sinensis, Ganoderma lucidum, dogwood and raspberry have the efficacy of tonifying the five internal organs, harmonizing six hollow organs, boosting spirit, improving appearance, tonifying kidney and securing essence, strengthening Yang and improving impotence, etc. With the characteristics of scientific compatibility and short brewing time, the health care wine is an ideal health beverage for enhancing the body's immune function, resisting aging, and prolonging life.