The invention discloses a uric acid test kit capable of eliminating drug interference of calcium dobesilate and etamsylate in serum and a preparation method thereof. According to the technical point,the test kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, peroxidase, ascorbic acid oxidase, a preservative, a Trinder's reactant A, metallate or a nonionic surfactant; and the reagent R2 comprises a buffer solution, serum albumin, uricase, a preservative and a Trinder's reactant B or a nonionic surfactant. According to the uric acid test kit disclosed by the invention, one or more than one of high-redox-potential oxidized metallate, vanadium pentoxide and vanadium oxychloride is / are added into R1 to be mixed, and calcium dobesilate and etamsylate are oxidized by utilizing the metallate, H2O2 generated by uricase in decomposing uric acid after R2 is added is prevented from being consumed, and an accurate uric acid measured value is rapidly obtained.