A uric acid kit capable of eliminating the interference of calcium dobesilate and fensulfate in serum and its preparation method
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A technology of calcium dobesilate and phenetamine, which is applied in the field of medical testing, can solve problems such as negative deviation, uric acid consumption, and misjudgment of doctors' diagnosis and treatment, and achieve the elimination of negative interference, high accuracy, precise drug use and The effect of treatment
Active Publication Date: 2021-02-02
ZHONGSHAN BGH BIOTECH CO LTD
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Calcium dobesilate is used as a commonly used drug for microvascular protection and treatment, and fensulfate is used as a commonly used drug for hemostasis. These drugs are all strong reducing agents. When patients taking these drugs measure plasma uric acid levels, these reducing drugs will H produced by the decomposition of uric acid by uricase 2 o 2 direct reaction, consumes H 2 o 2 Cause serious negative deviation of uric acid value, resulting in misjudgment of doctors in diagnosis and treatment
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Embodiment 1
[0063] Reagent R1 components:
[0064] Tris buffer 50mM pH8.0;
[0065] Peroxidase 9 ku / L;
[0066] Ascorbate Oxidase 6 ku / L;
[0067] Vanadium pentoxide 1 mM;
[0068] 4-AAP 3 mM;
[0069] TRITON X-100 0.2%;
[0070] Borax 1%
[0071] Reagent R2 components:
[0072] Tris buffer 50 mM pH8.0
[0073] Uricase 9 ku / L
[0074] Bovine Serum Albumin 0.1%
[0075] TOOS 10 mM
[0076] Borax 1.0%
[0077] TRITON 0.1%.
Embodiment 2
[0079] Reagent R1 components:
[0080] HEPES buffer 50mM pH8.0
[0081] Peroxidase 7.2 ku / L
[0082] Ascorbate Oxidase 6.4 ku / L
[0083] Sodium ferrate 1 mM
[0084] 4-AAP 3 mM
[0085] TRITON X-100 0.2%
[0086] Gentamicin Sulfate 0.1%;
[0087] Reagent R2 components:
[0088] HEPES buffer 50 mM pH8.0
[0089] Uricase 11.2 ku / L
[0090] Bovine Serum Albumin 0.1%
[0091] TOOS 10 mM
[0092] Gentamicin Sulfate 0.1%
[0093] TRITON 0.1%.
Embodiment 3
[0095] Reagent R1 components:
[0096] Phosphate buffer 50mM pH8.0
[0097] Peroxidase 4.6 ku / L
[0098] Ascorbate Oxidase 10 ku / L
[0099] Sodium metavanadate 2 mM
[0100] 4-AAP 3mM
[0101] TRITON X-100 0.2%
[0102] Sodium azide 0.1%;
[0103] Reagent R2 components:
[0104] Phosphate buffer 50mM pH8.0
[0105] Uricase 20 ku / L
[0106] Bovine Serum Albumin 0.1%
[0107] TOOS 10mM
[0108] Sodium azide 0.1%
[0109] TRITON X-100 0.1%.
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Abstract
A uric acid test kit capable of eliminating the interference of calcium dobesilate and phenetamine drugs in serum and a preparation method thereof of the present invention, the key points of its technical scheme are: comprising reagent R1 and reagent R2, and the reagent R1 contains buffer solution, peroxidase, ascorbate oxidase, preservative, Trinder's reaction reagent A, metal salt or nonionic surfactant; the reagent R2 includes buffer, serum albumin, uricase, preservative, and Trinder's reaction Agent B or nonionic surfactant. In the uric acid kit of the present invention, one or more of the oxidized metal salts of high redox potential or vanadium pentoxide or vanadium oxychloride are added to R1, and the hydroxybenzene Oxidation of calcium sulfonate and phenetamine prevents the consumption of H2O2 produced by uricase decomposing uric acid after adding R2, and quickly obtains accurate uric acid measurement values.
Description
technical field [0001] The invention belongs to the technical field of medical testing, and relates to a uric acid test kit capable of eliminating the interference of calcium dobesilate and phenetamine in serum and a preparation method thereof. Background technique [0002] Uric acid (Uric Acid, UA) is the final product of the metabolism of purine compounds in the body, which is weakly acidic. Uric acid can come from the body or from the catabolism of purines in food. In the body, uric acid is mainly produced in the liver, a small part can be excreted through the liver with bile, and most of the rest are excreted from the kidneys. The solubility of uric acid is small, so it is easy to precipitate crystals when the concentration in the body is high. Uric acid is considered to be closely related to the occurrence and development of gout, cardiovascular and cerebrovascular diseases, metabolic syndrome, ureteral stones and kidney diseases. In recent years, with the improvemen...
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