A rapid thin-layer identification method for Qiling granules with one test and multiple drugs

A detection method and particle technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of the detection personnel's health and adverse environmental effects, and achieve the effects of saving solvent and labor costs, simple operation, and quality control

Active Publication Date: 2022-04-08
CHIATAI QINGCHUNBAO PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above methods for simultaneous identification of Salvia miltiorrhiza and Gentiana miltiorrhiza all contain benzene in the developer, and benzene is a type of carcinogen, which has a great adverse effect on the health of the testing personnel and the environment.

Method used

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  • A rapid thin-layer identification method for Qiling granules with one test and multiple drugs
  • A rapid thin-layer identification method for Qiling granules with one test and multiple drugs
  • A rapid thin-layer identification method for Qiling granules with one test and multiple drugs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] TLC identification of Qiling Granules:

[0044] (1) Preparation of the test solution: Take 0.5 g of Qiling Granules, add 5 mL of methanol, ultrasonicate for 30 min, and take the supernatant to obtain the product.

[0045] (2) Preparation of reference solution: take salvianolic acid B, astilbin, and gentiopicroside, add methanol to make a mixture of salvianolic acid B, gentiopicroside each 1 mg / mL, and astilbin 1.5 mg / mL Reference substance solution, that is, too.

[0046] (3) Preparation of negative reference substance solution: Take 0.5 g of each negative particle, add 5 mL of methanol, sonicate for 30 min, and take the supernatant to obtain the product.

[0047] (4) Draw 4 μL of the test solution, 4 μL of the reference solution, and 4 μL of the negative control solution, and spot on the same silica gel GF 254 On a thin-layer board, use petroleum ether-ethyl acetate-methanol-anhydrous formic acid (2:7:1:0.5) as a developing agent, develop the filter paper in the deve...

Embodiment 2

[0050] The present embodiment investigates with different spotting methods and sample volumes of the test solution, to further illustrate the technical solution.

[0051] (1) Preparation of the test solution: Take 0.5 g of Qiling Granules, add 5 mL of methanol, ultrasonicate for 30 min, and take the supernatant to obtain the product.

[0052] (2) Preparation of reference solution: take salvianolic acid B, astilbin, and gentiopicroside, add methanol to make a mixed control of salvianolic acid B, gentiopicroside 1mg / mL, and astilbin 1.5mg / mL Product solution, that is.

[0053] (3) Preparation of the negative control solution: Take 0.5 g of each negative particle, add 5 mL of methanol, ultrasonicate for 30 min, and take the supernatant to obtain.

[0054] (4) Take 2 μL, 4 μL, 8 μL, and 10 μL of the different batches of the test solution, 4 μL of the reference solution, and 4 μL of the negative control solution, and spot on the same silica gel GF 254 On a thin-layer board, use p...

Embodiment 3

[0057] In this embodiment, the different sample volumes of the reference substance solution and the negative control solution are investigated to further illustrate the technical solution.

[0058] (1) Preparation of reference solution: take salvianolic acid B, astilbin, and gentiopicroside, add methanol to make a mixed control of salvianolic acid B, gentiopicroside 1mg / mL, and astilbin 1.5mg / mL Product solution, that is.

[0059] (2) Preparation of negative control solution: take 0.5 g of each negative particle, add 5 mL of methanol, ultrasonicate for 10 min, and take the supernatant to obtain the product.

[0060] (3) Preparation of the test solution: Take 0.5 g of Qiling Granules, add 5 mL of methanol, ultrasonicate for 10 min, and take the supernatant to obtain the product.

[0061] (4) Draw 1 μL, 3 μL, 5 μL of the reference substance solution, 2 μL, 4 μL, 8 μL, 10 μL of the negative control solution, and spot on the silica gel GF 254 On a thin-layer board, use petroleum...

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Abstract

The present invention relates to a kind of detection method of traditional Chinese medicine preparation, be specifically related to a kind of rapid TLC identification method of Qiling granule one test multidrug; Said detection method is thin-layer chromatography, comprises the following steps: (1) need testing solution Preparation: take Qiling granules, add methanol to extract; (2) Preparation of reference solution: take Qiling granules reference substance and add methanol to make reference solution; (3) Thin-layer chromatography identification: draw samples from each solution On the same silica gel thin-layer plate, a certain proportion of petroleum ether-ethyl acetate-methanol-anhydrous formic acid is used as the developing solvent, and the comparison can be carried out. Compared with the traditional thin-layer chromatography method, the biggest advantage of this method is that it can identify the three kinds of medicinal materials on the same thin-layer plate, without the need for chromogenic reagents, with low solvent toxicity, strong specificity, and good durability.

Description

technical field [0001] The invention relates to a detection method of traditional Chinese medicine preparations, in particular to a rapid thin-layer identification method of Qiling granule with one test for multiple medicines. Background technique [0002] Thin-layer chromatography is a commonly used method to identify the quality of traditional Chinese medicine products, and it can quickly separate and analyze various substances. Most traditional Chinese medicines and their compound preparations have complex ingredients, low content of index components, and many types of interfering substances. Therefore, independent TLC identification methods are mostly used for the identification of medicinal flavors of traditional Chinese medicine compound preparations. [0003] Conventionally, in the TLC identification of compound Chinese medicine preparations, there are multiple medicinal materials, and each medicinal material must be individually optimized for its test sample preparat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95
Inventor 应旭辉颜继忠陈忆刘雳姜慧洁张慧
Owner CHIATAI QINGCHUNBAO PHARMA
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