Two-photon fluorescence labeling probe, and synthesis method thereof and application of two-photon fluorescence labeling probe in novel coronavirus diagnosis
A two-photon fluorescence and labeling probe technology, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials, to achieve the effects of improving fluorescence quantum yield, simple structure, and low production cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0064] The synthetic method of two-photon fluorescent labeling probe LP, comprises the steps:
[0065] (1) Synthesis of 6-acetyl-2-naphthol (referred to as compound 2) (references are carried out): add 20 grams of 6-methoxyl-2-acetylnaphthalene (abbreviated as compound 1) and 200 ml of glacial acetic acid, stirred, then added 86 g of hydrobromic acid, and stirred and refluxed at 100°C for 12 hours, the black reaction solution was desolvated under reduced pressure at 60°C, neutralized by adding 10% sodium bicarbonate solution, and washed with ethyl acetate Extraction, organic phase with anhydrous MgSO 4 Drying, filtration, solvent removal under reduced pressure, column separation, 8.03g of 43.17mmol yellow powder is compound 2, yield 43%; wherein, the eluent used for column separation is V (petroleum ether): V (ethyl acetate )=2:1; the structure of the compound 2 is consistent with that reported in the literature (H.M.Kim, C.Jung, B.R.Kim, et al., Angew.Chem. Int.Ed., 2007,46:...
Embodiment 2
[0078] Example 2 Two-photon fluorescent labeling probe labeling N protein antigen Ag
[0079] Using Marsshall's method, the specific steps are as follows:
[0080] 1) Take 50 μL of 5 mg / mL N protein antigen solution, add 5 μL of carbonate buffer, 15 μL of 2 mg / mL LP-containing DMF solution in sequence, mix well, and react on a shaker at 200 rpm at 25°C for 2 hours; Carbonate buffer consists of Na 2 CO 3 8.6g,NaHCO 3 17.3g, prepared with 1000mL of distilled water, its pH is 9.3;
[0081]2) Purification by ultrafiltration: Take the liquid obtained in step 1) and add it to the ultrafiltration tube, add 0.02 mol / L, pH 7.2 phosphate buffer solution (PB) to 80% of the total volume of the ultrafiltration tube, and then in 4 ° C, 8000- Centrifuge at 10,000 rpm for 5-20 minutes, add PB repeatedly and centrifuge for 3-5 times to obtain the fluorescent antigen, namely LP-Ag precipitate.
Embodiment 3
[0082] Example 3 Immunochromatographic test strips for rapid detection of new coronavirus
[0083] The composition of the test strip: sample pad, binding pad, detection G line, detection M line, quality control C line, absorbent paper, PVC bottom plate; the sample pad contains blood filter membrane, and the binding pad is coated with the fluorescence obtained in Example 2. antigen.
[0084] The production method steps are as follows:
[0085] (1) Preparation of detection line and quality control line
[0086] Use Bio-Dot XYZ-3050 automatic film spraying machine to spray 1 mg / mL purified anti-human IgM secondary antibody, anti-human IgG secondary antibody, and goat anti-mouse IgG antibody on the nitrocellulose membrane, that is, the detection M line of the NC membrane, respectively. Detect the positions of the G line and the quality control C line at a scribing speed of 0.80 μL / cm, dry at 35°C for 5 hours, and seal and store at 4°C for later use;
[0087] (2) The binding pad...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap