48results about How to "Mature" patented technology

The invention discloses a young pig islet cell culture medium and an application method thereof. The culture medium is added into a basic culture medium (M199): 1-50 mM of glutathione, 1-50 mM of glutamine, ITS 0.01-1 % of ITS, 1-50 mM of nicotinamide, 0.1-20 micro-M of water-soluble vitamin E, 1-100 U / L of heparin, 0.01-0.5 mM of 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride and 0.01-0.1 mM of exenatide. Compared with the common media (1640, F10 and EGM2), the viability and maturation rate of islet cells in young pigs can be significantly improved.

The invention discloses a refraction-reflection type imaging spectrometer optical system based on a prism-grating-prism decomposition, which is characterized by comprising a field stop, a collimate mirror, a plane mirror, a decomposition assembly, a convergent mirror group and a optical filter which are arranged sequentially from an object to an image. The optical system is in refraction-reflection type, the collimate mirror is a concave spherical reflector, the convergent mirror group is a spherical lens group, the decomposition assembly is of a prism-grating-prism form, a prism and a grating are combined for correcting spectrum curvature of the decomposition assembly, the grating is a reflection type plane grating, and an angle is formed between the convergent mirror group and the decomposition assembly in the optical axis direction and used for adjusting residual spectrum curvature of the optical system. The refraction-reflection type imaging spectrometer optical system based on the prism-grating-prism decomposition has the advantages of low spectrum curvature, high optical efficiency, compact structure, simply processing and installing and adjusting procedures and flexibility in selecting spectrum segments.

The invention discloses a device and method for producing ammonium sulfate. The device comprises a neutralization and crystallization reactor, a thickener, a decanter, a centrifugal machine and a fluidized bed dryer which are connected through a pipeline, wherein the thicker comprises a settling section, a thickening section and an elutriating section which are sequentially connected from top to bottom; the decanter is horizontal and is divided into a light-phase area and a heavy-phase area by a baffle plate; the centrifugal machine is a horizontal double-stage piston pushing type centrifugal machine; the fluidized bed dryer is a horizontal multi-stage fluidized bed dryer, and the interior of the dryer is divided into a feeder drying area and a product cooling area; the neutralization and crystallization reactor comprises a cylinder body in which an inner circulation passage formed by a flow guiding cylinder, a backflow baffle plate and a stirrer is arranged, and an outer circulation passage formed by a crystallizer circulating pump is arranged outside the cylinder body. According to the method for producing the ammonium sulfate, the ammonium sulfate is produced by a neutralization and crystallization one-step method, so that the energy can be saved and the power consumption can be reduced; in addition, the flow can be shortened, and the investment is reduced.

The invention provides a cultivation method for 'Jinying' novel grape species. Hard branches of dozens of Kyoho grapes are selected as scions; tens of summer black grape trees are selected as stock and pollinizers; in May, the hard branches of the Kyoho grapes and the summer black grape trees are grafted, after survival, only one Kyoho grafting branch can be reserved on each tree, and the other branches are sheared off; in the current year, after to-be-grafted Kyoho grapes bloom, the Kyoho grapes are mated with blooming summer black grapes, and isolation pollination is performed; in the current year, the grafted Kyoho grapes on the summer black grape trees are fruited, seeds are taken and embedded into sandy soil, in the next March, seedling growing is performed, and in the current July, green branch transplanting is performed. The average fruit weight of the species is about 12 grams, and the sugar content is about 16-18%. The mature period is about 20 days ahead of the schedule of the Kyoho grapes and about 10 days later than the summer black grapes, and the problems that the Kyoho grapes are seriously different in size and hard in fruit bearing in the flowering phase are effectively solved. The species has the only defect that the species has no immunity to gray mold and the gray mold needs to be prevented one more time compared with other species.

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

The invention relates to the field of cell culture, in particular to a method for preparing high-purity induced pluripotent stem cell-derived human brain dopaminergic neurons. The invention provides a human dopaminergic neuron induction method and a related culture solution formula. The culture medium includes serum-free basal medium, cell additives, neurodevelopmental factors and inhibitors. Among them, the use of serum-free basal medium can avoid the introduction of exogenous substances and reduce the risk of contamination. At the same time, a variety of nutrients, such as cell additives and neurodevelopmental factors, can stimulate iPSC cells to activate iPSC cells and promote differentiation. The cell culture method provided by the invention is simple, efficient, low in cost, and can induce high-quality human dopaminergic neuron cells in a short period.