39results about How to "Low requirements for detection conditions" patented technology

The invention discloses a detecting method for tobacco package and its setting. The method adopts the X ray linear scan technology to detecting the defects of packing, rolling and producing in the tobacco packaging process, which mainly is :appending a long narrow aperture in front of the X-ray generator so as to make the X ray forming a thin fan-shaped planar beam after transmitting it, adopting this fan-shaped planar beam to linearly scan the tobacco package, and using a detector which can receive X ray to receive the transmitted X-ray directly, the clairvoyance digital image can be receive by computer after processing, so as to recognize the short of bars or packs in a whole box, of packs or number in a whole bar, of number in a whole pack and other quality problems produced in the packing and rolling process so as to remove the unqualified products with eliminating equipment. The principle and structure of the invention is more rational and simple, high sensitive and capable of detecting dynamically compared to current techniques.

The invention provides a kit for detecting enterocytozoonhepatopenaei and a method for detecting whether aquatic foods contain enterocytozoonhepatopenaei. Besides a primer pair, the kit further comprises nucleic acid extracting liquid. The nucleic acid extracting liquid is formed by 4% of sodium dodecyl sulfate (SDS) and 1.5M Tris / HCl (at the pH of 8.8). An established TaqMan real-time real-time fluorescence quantification polymerase chain reaction (PCR) method has a detection reaction sensitivity as high as 5.20*102 copies per microliter. Compared with ordinary PCR, the sensitivity of the method is 10 times higher than that of the conventional PCR.

The invention discloses an antibody matrix detection method (MEGA method) and a multiplex detection card for pathogen antigens. The invention provides an antibody multi-linked detection card for detecting or assisting in detecting pathogen antigens of a detected sample. The antibody multi-linked detection card is formed by connecting n detection test strips for detecting different antibodies in parallel, each detection test strip comprises a sample pad, a combination pad sprayed with a detection antibody, an NC membrane containing m detection lines and a quality control line, and absorbent paper along the flowing direction of the detected sample; m is greater than or equal to 1. The MEGA method and the multiplex detection card thereof can be used for simultaneously detecting different types and subtypes of antibodies generated by a subject aiming at various antigens of pathogens, and the obtained data can more accurately reflect the conditions of infection and protection. The MEGA method and the multiplex detection card thereof can be used for serum detection, and can also be used for noninvasive detection of other body fluids, especially mucous membrane fluids.

The invention discloses a full-film structure electromagnetic wire thin-film adhesion detection method for a motor coil. The full-film structure electromagnetic wire thin-film adhesion detection method is divided into two parts: a twisting test and a soaking test, which are performed in turn. The full-film structure electromagnetic wire thin-film adhesion of the motor coil is judged through the twisting test and soaking test. The twisting test comprises the following steps: intercepting two sections of a finished product electromagnetic wire with the length between 100 and 200mm; clamping and fixing one end of the electromagnetic wire respectively, and twisting 360 degrees for five times after clamping the other end of the electromagnetic wire; and clockwise twisting a test piece, anticlockwise twisting another test piece, and observing whether the electromagnetic wire thin-film is in glue failure. The soaking test comprises the following steps: soaking the twisted part in water for about 4 to 10 minutes; and using 1,000V insulated resistance tester to measure that the insulated resistance between a copper conductor and external insulation is not less than 10 megohms. The full-film structure electromagnetic wire thin-film adhesion detection method has the characteristics that: the electromagnetic wire thin-film adhesion detection method is performed through the twisting test and soaking test respectively, so the requirement on the detection condition of the detection is reduced.

The invention discloses a method for specifically and semiquantitatively detecting benzopyrene, which comprises the steps of antigen synthesis, antigen fixation, benzopyrene detection and detection signal acquisition. The key points are that a chemiluminescence substrate added with horseradish peroxidase reacts in a darkroom, a signal is developed and exposed by using a photosensitive film and the detection signal is analyzed. In addition, the invention also gropes a condition suitable for detection. Compared with the general method for detecting the benzopyrene, the method has the advantages of simple and convenient process, rapidness, practicability, no need of any instrument, cost saving and accurate result and provides a new path for detecting the benzopyrene in the environment.

The invention discloses an artificial-intelligence-based abnormal eyelid movement evaluation system. The system comprises an examined object obtaining module, an eye and specific part positioning module, a TSN model, a probability output module and an evaluation module; the examined object obtaining module is used for obtaining a facial video which is obtained from a facial movement video of the input examined object and only contains the examined object, the eye and specific part positioning module is used for positioning the eye and specific part of the facial video and obtaining an eye movement video and a specific part linkage video which only contain the examined object, the TSN model is used for processing the eye movement video and the specific part linkage video and outputting movement signals of eyes and specific parts, the probability output module is used for outputting probability signals appearing when a computer judges that eyelid movement is abnormal in each frame, and the evaluation module is used for obtaining the abnormal level according to the probability signals appearing in abnormal eyelid movement and an abnormal eyelid movement probability judging mechanism.Accordingly, universal acceptability, convenience, accuracy, objectivity and repeatability are achieved, and the clinical applicability is high.

The invention discloses a kit and method for detecting anaplasmosis and rickettsioses based on LAMP. The invention relates to a molecular biological detection technology for anaplasmosis and rickettsioses, provides specific primers which are designed to be directed to ompB gene of spotted fever group rickettsia (SFGR) and msp2 gene of anaplasma phagocytophilum, and provides a detection kit for SFGR and anaplasma phagocytophilum by using LAMP. The kit has the characteristics of rapidness, sensitivity, high specificity, no experimental condition restrictions, etc., and is applicable to clinicaldetection for spotted fever and granulocytic anaplasmosis, especially in rural areas where medical conditions are limited. The invention also discloses a method for detecting rickettsioses with the kit.

The invention discloses an isolation pad detecting device and method. The method includes: producing the X ray penetrating a substrate with an isolation pad, receiving the X ray, and forming a grayscale image; using the grayscale image to measure the height of the isolation pad. The method has the advantages that the X ray imaging principle is used to detect the isolation pad on the color film substrate, an X ray fluorescent light converting device is used to produce visible light and generate the grayscale image, the grayscale image is identified and processes to accurately measure the height of the isolation pad, and the internal and connecting states of the isolation pad can also be detected.

The invention discloses a detection tool for measuring position accuracy of a shaft outer wall oblique hole. The detection tool comprises a base, a linear rail, a tail base, a pin base, a sliding plate, a right bearing, an edge cutting pin, a gauge frame, a clock gauge and a left bearing, wherein the sliding plate is matched with the oblique pin base to move left and right along the linear rail, so that an oblique hole in a conical shaft section of a workpiece can be aligned; the edge cutting pin is fixedly connected with the left end surface of the sliding plate; the vertical edge cutting pinis mounted in a manner of enabling the cut edge to be arranged outwards; the gauge frame is arranged on one side of the linear rail; the upper end of the gauge frame is used for clamping the clock gauge with an upward gauge disc; a gauge head end of the clock gauge faces rightwards and is in contact with the plane of the edge cutting pin to form a positioning and measuring mechanism. The detection tool is simple in structure and high in detection accuracy, is capable of efficiently detecting the position accuracy of the shaft outer wall oblique hole, and is especially applied to on-line detection of production site.

The invention provides a leakage point detecting device for a clean liner container, which belongs to the technical field of detecting devices for detecting leakage points of the clean liner containers. The clean liner container comprises a cylinder body, a coating and a manhole, wherein the coating is coated at the inner wall of the cylinder body; the manhole is formed on the cylinder body and used for an operator to pass in and out of a cylinder cavity; the leakage point detecting device for the clean liner container is characterized by comprising a resistance meter, a first lead, a second lead and a wet tissue, wherein one pole of the resistance meter is connected with the outer wall of the cylinder body by the first lead; the other pole of the resistance meter is connected with the wet tissue by the second lead; and the wet tissue is in contact with the surface of the coating. The leakage point detecting device for the clean liner container, provided by the invention, has the advantages of simple structure, low requirement on detection conditions, low utilization cost, simplicity in operation, high leakage point identification speed, high accurate rate, no requirement on the surface area of the inner wall of the cylinder body, wide application range and incapability of causing damage to the coating in the operation process, and can be operated only by a common person; and within a short time, all possible leakage points can be found in the liner container.

The invention provides polypeptide capable of being specifically bound with tuberculosis-positive serum. The polypeptide is one or a combination of A and B, wherein the amino acid sequence of the A is shown as SEQ ID NO: 1; the amino acid sequence of the B is shown as SEQ ID NO: 2. The invention further provides a serological tuberculosis diagnosing kit. The kit contains the polypeptide specifically bound with the tuberculosis-positive serum. The invention further provides a tuberculosis diagnosing kit based on phage display polypeptide. The kit contains a core phage P1 and a core phage P4, wherein the core phage P1 is phage of display polypeptide A; the core phage P4 is phage of display polypeptide B. The polypeptide and the corresponding core phages provided by the invention are high in sensitivity and specificity, and have a good clinical diagnostic value.

The invention discloses a tool for detecting the linear distance between an inner hole annular groove and an outer end face of a gear. The tool comprises a pipe plug, an inner axle sleeve, springs, a measuring sleeve, a cylindrical pin, an outer sleeve, a push rod, a gauge stand and a dial indicator. An end, provided with external thread, of the inner axle sleeve is connected with the outer sleeve and the gauge stand. An indicator rod of the dial indicator is inserted into an inner hole of the gauge stand. An inner hole of the measuring sleeve is in clearance fit with the inner axle sleeve. Annular bosses are arranged on the outer walls of the two ends of the measuring sleeve correspondingly. The annular boss stretching into an inner hole of a workpiece is a measuring end. The annular boss at the other end is connected with two handles stretching out radially. The cylindrical pin is stuffed radially into the tube wall in the middle section of the measuring sleeve. The push rod is placed and inserted into one inner hole of the inner axle sleeve. One spring is axially arranged between the push rod and the gauge stand. One spring is also axially arranged in an inner hole in the other end of the inner axle sleeve and is adjusted and locked in position through the pipe plug. The to-be-measured workpiece is stuffed into the detecting tool for measurement, direct measuring is good in promptness and high in precision, and the detecting tool is suitable for being used in fields of production.

The invention discloses a testing tool for measuring the tooth thickness of a heavy-duty spiral bevel gear on line, which comprises a ball head, an indicator clamp, a dial indicator, a movable clamp,a vertical rod, a slide rod, and a base. Both the vertical rod and the slide rod are cylindrical straight rods, and the vertical rod is articulated with the slide rod through the movable clamp on oneside. The movable clamp, which is a tightness-adjustable clamp, is used for changing the locking position and bending angle of the clamped slide rod. The extended end of the slide rod is directly articulated with the indicator clamp, and the dial indicator, the meter of which is downward, is inserted in the horizontal indicator clamp. The meter of the dial indicator can be freely extended and retracted, and the ball head is stuck at the end of the meter. The bottom end of the vertical rod is fixedly connected to the angled base. The testing tool is a rod type testing tool, the structure is simple and light, moreover, the requirement on detection conditions is not high, above all, a workpiece does not need to be moved during measurement, and therefore the testing tool is suitable for on-line detection on the production site. Because only one person is needed to use the testing tool for detection, the testing tool can save manpower in comparison with the prior art, and thereby the production cost can be remarkably reduced.

The invention discloses a tobacco root-knot nematode disease pathogen detection kit based on a loop-mediated isothermal amplification technology and an application method of the tobacco root-knot nematode disease pathogen detection kit. The tobacco root-knot nematode disease pathogen detection kit based on the loop-mediated isothermal amplification technology comprises a reaction solution I for detecting meloidogyne incognita, a reaction solution A for detecting meloidogyne arenaria, a reaction solution J for detecting meloidogyne javanica, a BstDNA polymerase klenow fragment and a 100*SYBR Green I staining solution. The tobacco root-knot nematode disease pathogen detection kit is based on the loop-mediated isothermal amplification technology, has low requirements on detection conditions,can implement the detection only with a water bath pot or a thermostat and facilitates the popularization and application. Since the kit and the method do not need gel electrophoresis, a detection result can be directly observed by naked eyes, so that the consumed time is short, and the detection can be completed in 1.5h; and the kit comprises an LAMP detection primer for detecting the meloidogyneincognita, the meloidogyne arenaria and the meloidogyne javanica, so that the three common pathogen root-knot nematode of tobacco root-knot nematode disease can be synchronously detected.

The invention discloses a method for detecting the concentration of zinc ions. The method comprises the following steps: preparing a glassy carbon electrode, of which the surface is modified with an amino-graphene and beta-cyclodextrin composite material; adding a to-be-detected solution into a buffer solution to obtain a to-be-detected mixed solution; and detecting the to-be-detected mixed solution with a three-electrode system, and obtaining the concentration of zinc ions in the to-be-detected mixed solution according to a square wave stripping voltammetry curve by reference to a zinc ion standard linear equation, wherein a working electrode in the three-electrode system is the glassy carbon electrode, of which the surface is modified with amino-graphene and beta-cyclodextrin. The method disclosed by the invention is simple, is easy to operate, has low requirements on detection conditions, greatly increases the sensitivity of zinc ions, and is up to the zinc ion detection limit of 2*10<-7> mol / L.

The invention discloses a method for detecting the concentration of cadmium ions. The method comprises the following steps: preparing a glass carbon electrode of which the surface is provided with modified aminated graphene and a beta-cyclodextrin composite material; adding a to-be-detected solution into a buffer solution to obtain a to-be-detected mixed solution; detecting the to-be-detected mixed solution by using a three-electrode system; obtaining a voltage-current curve according to square wave of the cadmium ions; acquiring the concentration of the cadmium ions in the to-be-detected mixed solution according to a cadmium ion standard linear equation, wherein each working electrode in the three-electrode system is the glass carbon electrode of which the surface is provided with modified aminated graphene and beta-cyclodextrin. The method disclosed by the invention is simple, easy to operate and low in requirements for detection conditions and has the effect of greatly improving the sensitivity of the cadmium ions. The detection limit of the method on the cadmium ions is as high as 1.1x10<-7>mol / L.

The invention relates to a detection method for detecting hydrargyrum contained in removing-freckle cream, which is characterized in that a CuI reagent is prepared; a small amount of removing-freckle cream is plastered on filter paper and the CuI test solution dropped on the paper and the phenomenon is observed; if the colour of henna or salmon pink appears, the ammoniated mercuric chloride in the removing-freckle cream severely exceeds the standard. The method of the invention has simple operation and quick detection speed and has low detection condition requirement; the quality legal operation department can complete the detection of the hydrargyrum contained in the removing-freckle cream in site; furthermore, the test result has strong specificity and high exactness.

The invention provides a gait detection device based on plantar pressure. The gait detection device comprises a sole plate, a sole air bag and a signal processing unit, wherein a magic tape is arranged on the foot bottom plate and is used for fixing the foot of the target object on the foot bottom plate; a plurality of plantar air bags are arranged, and the plurality of plantar air bags are not communicated with one another and are arranged on the bottom surface of the plantar plate in a matrix manner; an air pressure detection hole is formed in each plantar air bag, and an air pressure sensoris arranged at each air pressure detection hole so as to detect the air pressure in the corresponding plantar air bag when the plantar air bag is pressed to deform in the walking process of the target object; a signal processing unit is located on the sole plate, electrically connected with the air pressure sensor and used for processing a pressure signal, detected by the air pressure sensor, ofthe sole air bag. The deformation of the plantar air bag of the device does not drive the stress deformation of the air pressure sensor and is not influenced by the ectropion or ectropion of the anklejoint of the target object, the requirement on the detection condition is reduced, and the service life is prolonged.

The invention discloses a mechanical part hole inner ring groove width dimension quantitative measurement device, which comprises a screw plug, a spring, an inner pipe, a slide block, a measuring rod, an outer pipe, a meter seat and a dial indicator, wherein an annular radial groove is formed in the outer wall of one end, connected with the meter seat, of the inner pipe; the other end of the inner pipe is provided with two movable measuring heads arranged at intervals; a measuring rod arranged in the inner pipe axially penetrates through the slide block and is tightly locked; the spring arranged on the slide block is axially regulated by the screw plug. The outer pipe sleeves the outer wall of the inner pipe; one end positioned at the meter seat is matched with the radial groove formed in the outer all of the inner pipe through a stop head screw to form a position limiting rotating structure; a static measuring head opposite to the position of the stop head screw is arranged on the outer wall of the other end of the outer pipe; the slide block extending out from the rectangular radial through hole pre-installed in the outer wall of the inner pipe is axially linked with the outer pipe, so that the movable measuring head of the measuring rod moves relative to the static measuring head of the outer pipe. Therefore the measuring structure of directly measuring the workpiece hole inner ring groove width by the dial indicator is formed. The measurement device provided by the invention has the advantages that the structure is simple; the measurement is convenient and fast; the device is directly used for online measurement.

The invention relates to a detection method for detecting overproof hydrargyrum contained in removing-freckle cream, which is characterized in that a small amount of DLZ-1 powder is dissolved in ethanol so as to prepare a DLZ-1 test solution; the removing-freckle cream is plastered on filter paper and the DLZ-1 test solution dropped on the paper; the filter paper is irradiated under an ultraviolet lamp and the phenomenon is observed; if fluorescence exists, the ammonium chloride based hydrargyrum exceeds the standard. The method of the invention has simple operation and quick detection speed and has low detection condition requirement; the quality legal operation department can complete the detection of the hydrargyrum contained in the removing-freckle cream in site; furthermore, the test result has strong specificity and high exactness.

The invention belongs to the technical field of rapid detection of heavy metals, and discloses an ion imprinted material for rapid color development and detection of copper ions and a preparation method and application thereof. The preparation method comprises the following steps: dissolving diphenyl semicarbazide and CuCl2 in a proper amount of an ethanol solution according to a molar ratio of 2:1, carrying out ultrasonic treatment until the diphenyl semicarbazide and CuCl2 are completely dissolved, and stirring and pre-polymerizing at room temperature for 3 hours to obtain a solution 1; adding azodiisobutyronitrile and ethylene glycol dimethacrylate into the toluene solution, and mixing and dissolving to obtain a solution 2; mixing the two solutions, and adding a hydroxyethyl cellulose solution; oscillating for 6 hours at the temperature of 70 DEG C under the oxygen-free condition of introducing N2; and washing, eluting and drying to obtain the target material. According to the invention, an ion imprinting technology and a chemical developing technology are organically combined to obtain the developing imprinting material capable of rapidly and specifically detecting trace copper ions. The preparation method is simple, good in selectivity and reusable; and the method is high in color development speed, can complete detection within one minute, is reliable in result, and can be used for quickly detecting copper ions in an actual water sample.

The invention relates to the technical field of electrolyte component determination, in particular to a method for detecting the concentration content of lithium hexafluorophosphate in an electrolyteby using ICP. The method comprises the following steps: taking 100mL of 37% concentrated hydrochloric acid, and adding 100mL of deionized water to dilute for later use to prepare a hydrochloric acid solution; accurately taking 20mL of a solvent to be detected into a 125mL separating funnel by adopting a pipette or a pipette gun; adding 30 mL of a hydrochloric acid solution, oscillating, standing for 10 min to obtain a water phase, namely a lithium salt solution obtained by extraction, carrying out back extraction on the organic phase twice by using hydrochloric acid, mixing and collecting thewater phase lithium salt solution obtained by three times of extraction, and meanwhile, fixing the volume to 100 mL by using deionized water to obtain an electrolyte to be detected; performing plasmaemission spectrum analysis on the to-be-detected electrolyte by using a plasma emission spectrum analyzer to obtain absorbance values A of the to-be-detected electrolyte, solving the lithium salt concentration C0 of the diluted to-be-detected electrolyte according to the absorbance values A and the standard curve, and calculating the lithium salt concentration C of the to-be-detected electrolyte according to a formula. The method is simple to operate, and the efficiency and precision are improved.

The present invention provides a polypeptide that can specifically bind to Mycoplasma pneumoniae positive serum, and the amino acid sequence of the polypeptide is shown in SEQ ID NO: 1: T-V-N-F-K-L-Y. The present invention also provides a serological diagnostic kit for Mycoplasma pneumoniae infection, which contains the above-mentioned polypeptide specifically binding to Mycoplasma pneumoniae positive serum. In addition, the present invention also provides a phage-displayed polypeptide-based diagnostic kit for Mycoplasma pneumoniae, the kit includes a core phage, wherein the core phage is a phage displaying the above-mentioned polypeptide. The polypeptide and its corresponding core phage provided by the present invention have high sensitivity and specificity, and have good clinical diagnostic value.

The invention relates to a method for detecting drugs by combining liquid-phase extraction and fluorescence sensing technologies. The method comprises the steps of firstly, treating a test sample in aliquid-phase extraction mode to ensure that the drugs can be enriched, purified and extracted, and the chemical structure of the drugs in the sample can be prevented from being damaged; and therefore, when the drugs are further detected on the basis of a fluorescence sensing technology, outputting an accurate detection result. Moreover, the fluorescence sensing technology has the advantages of high sensitivity, good selectivity, rich response signals, simplicity in operation, simple detection conditions, lower requirements on instruments and the like, and compared with a traditional detectionmethod, the fluorescence sensing technology is easier to miniaturize devices and can be used for quickly detecting the drugs in real time.

The invention relates to a thyroid hormone disrupter detection method and a detection kit, and the detection method comprises the following steps: fixing a yeast on the surface of a culture medium, the yeast containing a thyroid hormone receptor gene, a co-activator gene and a reporter gene; adding a sample to be detected to the surface of the culture medium to react with the yeast; breaking cell walls of the yeast acted on the to-be-detected sample; and detecting the expression level of the reporter gene in the yeast. According to the method for detecting the interference effect of the environmental thyroid hormone of the immobilized yeast, the yeast cells with relatively high density can be directly fixed and covered on the surface of the culture medium, sample testing can be carried out only by directly adding an environmental sample within the storage period of 90 days, water sample pretreatment and temporary yeast cell pre-culture are not needed, and the detection cost is reduced. The detection condition requirements are greatly reduced, and the method can be suitable for field on-site detection.

The invention provides a recombinant antigen protein rP44-60 for detecting granulocytoplasmosis and a kit containing the antigen, and belongs to the technical field of immunology, and the recombinant antigen protein comprises an amino acid sequence as shown in SEQ ID NO.1 in a sequence table. The kit comprises a recombinant antigen protein rP44-60, a sample pad, a combination pad, an absorption pad, a nitrocellulose membrane, a colloidal gold probe, mouse IgG and goat anti-mouse IgG. The recombinant antigen protein disclosed by the invention is high in sensitivity and strong in specificity, and can be used for rapidly detecting an antibody in anaplasma phagocytophilum infected serum, so that the problem that the antibody is difficult to capture due to antigen variation of HGA is solved, the defect of existing antigen missing detection is made up, and the missed diagnosis rate is effectively reduced; according to the preparation method, cross reactivity caused by irrelevant sequences is reduced, and the expression quantity and stability of recombinant antigen protein are high; the kit has the characteristics of rapidness, sensitivity, high specificity, no limitation of experimental conditions and the like, and has low requirements on detection conditions.