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Antibody matrix detection method (MEGA method) and multiplex detection card for pathogen antigens

A pathogen and detection card technology, which is applied in the field of antibody matrix detection method and multiple detection cards, can solve the problems of inability to indicate pathogens and lag, and achieve the effect of reducing psychological barriers and infection risks, lowering requirements, and facilitating self-testing

Pending Publication Date: 2020-06-30
陈韬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the indirect method is that it takes several days to produce specific antibodies after the pathogen is infected, and there will be a certain lag compared with the direct method
Since the antibody can still maintain a high level for a period of time after the pathogen is cleared, the positive result obtained by the indirect method can only indicate that the subject has been infected by the pathogen, but it cannot indicate whether the pathogen still exists in the subject during the test. in vivo

Method used

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  • Antibody matrix detection method (MEGA method) and multiplex detection card for pathogen antigens
  • Antibody matrix detection method (MEGA method) and multiplex detection card for pathogen antigens
  • Antibody matrix detection method (MEGA method) and multiplex detection card for pathogen antigens

Examples

Experimental program
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Effect test

Embodiment 1

[0079] Example 1. Preparation of antibody rapid detection matrix method (MEGA method) against pathogen antigen and its special multi-link detection card

[0080] 1. The structure of the multi-link test card

[0081] The multi-link test card of the rapid antibody detection matrix method (MEGA method) for pathogen antigens is composed of n test strips of different detection antibodies in parallel (e.g. figure 2 (Shown); each test strip includes a sample pad (with a sample hole), a binding pad sprayed with a detection antibody, m (greater than or equal to 1) test lines, and NC film and absorbent paper of the quality control line;

[0082] The detection antibody on the binding pad in each test strip in the multi-link test card is selected from any one of n (blood test n is greater than or equal to 3, mucosal fluid test n is greater than or equal to 1), and is not repeated.

[0083] The n kinds of antibodies are any one of the following: n kinds of human immunoglobulins or subclasses the...

Embodiment 2

[0141] Example 2. Preparation and application of multi-link test card for detecting new coronavirus COVID-19

[0142] 1. Preparation of multiple test cards for the detection of COVID-19

[0143] Prepare according to the second method of Example 1:

[0144] 1. Preparation of test strips

[0145] 1), sample pad

[0146] Same as the second method of Example 1, using glass fiber;

[0147] 2) Prepare binding pad sprayed with detection antibody

[0148] (1) Colloidal gold-labeled detection antibody

[0149] The detection antibodies are IgG monoclonal antibody (KT131, Beijing Aibosheng Biotechnology Co., Ltd.), IgA monoclonal antibody (KT13, Beijing Aibosheng Biotechnology Co., Ltd.), IgM monoclonal antibody (KT194, Beijing Aibosheng Biotechnology Co., Ltd.) Technology Co., Ltd).

[0150] The preparation method of the colloidal gold-labeled detection antibody is the same as that of Example 1, using glass fiber to obtain a binding pad sprayed with IgG detection antibody, a binding pad sprayed with...

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Abstract

The invention discloses an antibody matrix detection method (MEGA method) and a multiplex detection card for pathogen antigens. The invention provides an antibody multi-linked detection card for detecting or assisting in detecting pathogen antigens of a detected sample. The antibody multi-linked detection card is formed by connecting n detection test strips for detecting different antibodies in parallel, each detection test strip comprises a sample pad, a combination pad sprayed with a detection antibody, an NC membrane containing m detection lines and a quality control line, and absorbent paper along the flowing direction of the detected sample; m is greater than or equal to 1. The MEGA method and the multiplex detection card thereof can be used for simultaneously detecting different types and subtypes of antibodies generated by a subject aiming at various antigens of pathogens, and the obtained data can more accurately reflect the conditions of infection and protection. The MEGA method and the multiplex detection card thereof can be used for serum detection, and can also be used for noninvasive detection of other body fluids, especially mucous membrane fluids.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to an antibody matrix detection method (MEGA method) and a multi-link detection card for pathogen antigens. Background technique [0002] During the outbreak of infectious diseases, effective rapid detection is extremely important for patient isolation and disease control. Among various methods of rapid detection, lateral chromatography based on immunological principles is a commonly used method. [0003] Among various diseases, respiratory tract infection is a particularly important public health problem. Respiratory tract infections are mainly caused by viruses, bacteria, mycoplasma, and chlamydia, which infect upper respiratory tract organs such as nasal cavity, pharynx, larynx, and lower respiratory tract organs such as trachea, bronchus, and lungs. The main pathogens include rhinovirus, coronavirus, influenza virus, adenovirus, Streptococcus pneumoniae, Haemophilus influenzae, Chlamydia p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/569
CPCG01N33/558G01N33/56983G01N2333/165
Inventor 陈韬
Owner 陈韬
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