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Chlamydia pneumoniae detection reagent card and kit and detection method

A technology for Chlamydia pneumoniae and detection reagents, which is applied in measuring devices, instruments, and disease diagnosis. It can solve the problems of large sample size, inconvenient detection for users, and inconvenient operation. It achieves small sample size, easy judgment, and easy operation. Effect

Inactive Publication Date: 2017-03-29
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the prior art, the detection of Chlamydia pneumoniae is mostly done by taking blood samples, diluting the blood samples, and then adding the diluted samples to the test strips for detection. This method usually requires a large amount of samples and requires Professionals perform operations such as blood drawing and dilution, which is not convenient for users to perform self-testing at home and other places, and the operation is inconvenient

Method used

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  • Chlamydia pneumoniae detection reagent card and kit and detection method
  • Chlamydia pneumoniae detection reagent card and kit and detection method
  • Chlamydia pneumoniae detection reagent card and kit and detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Chlamydia pneumoniae detection reagent card

[0030] This embodiment provides a Chlamydia pneumoniae detection reagent card and a kit including the reagent card. As shown in the figure, the reagent card 1 includes a housing 11 and a test strip 2. The housing 11 is sequentially provided with a diluent filling hole 12, a sample filling hole 13, and a result observation window 14; the test strip 2 includes a bottom plate 23 And the sample pad 21, the binding pad 22, the nitrocellulose membrane 24, the absorbent paper 26 lapped successively on the bottom plate 23; the diluent liquid filling hole 12 and the sample sample filling hole 13 correspond to the test strip sample pad 21, and the result The observation window 14 corresponds to the nitrocellulose membrane 24 . The chlamydia pneumoniae detection reagent card is coated with chlamydia pneumoniae recombinant antigen detection line 25 and anti-mouse IgM antibody quality control line 26 on the nitrocellulose...

Embodiment 2

[0032] Example 2 Chlamydia pneumoniae detection kit

[0033] This embodiment provides a Chlamydia pneumoniae detection kit, including the detection reagent card 1 of Embodiment 1, a sample pipette 3 and a sample diluent 4 .

[0034] When using the kit of the present embodiment to detect, the operation steps are as follows:

[0035] (1) Take out reagent card 1 from the kit and place it horizontally;

[0036] (2) Take the serum or plasma sample with the straw 3, and add 10ul vertically to the sample hole 13;

[0037] (3) Add 2-3 drops of diluent to the diluent filling hole 12;

[0038] (4) Wait for 10 minutes and observe the result.

[0039] If the detection line 25 and the quality control line 26 develop color at the same time, it means that there is Chlamydia pneumoniae antigen in the sample, and the patient is infected by Chlamydia pneumoniae;

[0040] If the test line 25 does not develop color and the quality control line 26 develops color, it means that there is no...

Embodiment 3

[0042] Example 3 Chlamydia pneumoniae detection reagent card

[0043] The difference between this embodiment and embodiment 1 is that the distance between the detection line 25 and the quality control line 26 is 5 mm, and the quality control line 26 is coated with anti-rabbit IGM antibody.

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Abstract

The invention discloses a chlamydia pneumoniae detection reagent card. The chlamydia pneumoniae detection reagent card is characterized in that the reagent card comprises a shell and a test paper strip. The shell is orderly provided with a dilution liquid feeding hole, a sample feeding hole and a result observation window. The test paper strip comprises a baseplate, and a sample pad, a bonding pad, a nitrocellulose film and a water absorbent paper orderly arranged on the baseplate in an overlap joint way. The dilution liquid feeding hole and the sample feeding hole are corresponding to the sample pad of the test paper strip. The result observation window is corresponding to the nitrocellulose film. The reagent card is provided with the sample feeding hole and the dilution liquid feeding hole. A developer needs to drop 1-2 drops of a blood sample in the sample feeding hole and then drop a dilution liquid in the dilution liquid feeding hole to complete dilution and sample extraction and dilution are avoided so that the operation is very convenient, the required sample amount is very small and the cost is saved.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a chlamydia pneumoniae, a chlamydia pneumoniae joint detection reagent card, a kit and a detection method thereof. Background technique [0002] Chlamydia pneumoniae (Chlamydia pneumoniae, Cpn) is a new species named in 1989, it has only one serotype, MOMP protein is its specific antigen, and the representative strain is TWAR. At present, more than 10 strains of Chlamydia pneumoniae have been newly isolated, which is an important respiratory pathogen. [0003] Chlamydia pneumoniae respiratory infection is a common disease widespread in the world, causing endemic and epidemic pneumonia, no significant gender and regional differences, can occur all year round, almost everyone has been infected in his life, and often repeated infections; The distribution of infections is bimodal, with the first peak at 8-9 years of age and the second at 70 years of age. The incubation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56927G01N2800/12
Inventor 王继华
Owner GUANGZHOU WONDFO BIOTECH
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