Mycoplasma pneumoniae and Chlamydia pneumoniae combined detection reagent card, and Mycoplasma pneumoniae and Chlamydia pneumoniae combined detection kit and detection method thereof

Abstract

The present invention provides a Mycoplasma pneumoniae and Chlamydia pneumoniae combined detection reagent card, and a Mycoplasma pneumoniae and Chlamydia pneumoniae combined detection kit and a detection method thereof. The Mycoplasma pneumoniae and Chlamydia pneumoniae combined detection reagent card comprises a shell and a test paper strip, wherein the shell is sequentially provided with a dilution liquid adding hole, a sampling adding hole and a result observation window, the test paper strip comprises a bottom plate, a sample pad, a bonding pad, a nitrocellulose film and a water absorption paper, the sample pad, the bonding pad, the nitrocellulose film and the water absorption paper are sequentially overlapped on the bottom plate, the dilution liquid adding hole and the sample adding hole are corresponding to the sample pad of the test paper strip, the result observation window is corresponding to the nitrocellulose film, and at least a detection line and a quality control line are coated on the nitrocellulose film. According to the present invention, with the reagent card, the kit and the detection method, the one-time combined detection on Mycoplasma pneumoniae and Chlamydia pneumoniae can be achieved, the complete detection is easily achieved, the miss of the detection can be avoided, the detection result can well guide the clinical diagnosis and the correct medication, and the clinical diagnosis efficiency can be improved; and with the combined detection, the sample can be easily saved, and the invasive pain of the detection on the patient can be reduced.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a combined detection reagent card, kit and detection method for Mycoplasma pneumoniae and Chlamydia pneumoniae. Background technique [0002] Mycoplasma pneumoniae (Mycopplasma pneumonia, MP) belongs to the genus Mycoplasma of the Mycoplasma family of the Mollicula class, and is a kind of prokaryotic microorganism without cell walls that can grow and reproduce by itself on a non-living medium, and is between viruses and bacteria. Mycoplasma pneumoniae grows slowly, the culture period is long, and it is difficult to isolate and culture in vitro. P1 protein is a pair of trypsin-sensitive transmembrane proteins in the top structure of MP, and is also the main adhesin of Mycoplasma pneumoniae. Its relative molecular weight (Mr) is about 170x103. In the absence of P1 or P1 can not be dense in the appropriate position on the surface of the apical organ or non-resident apic...

AI Technical Summary

Problems solved by technology

[0010] Most of the existing detection and diagnosis technologies are single detection for Mycoplasma pneumoniae or Chlamydia pneumoniae, but no joint detection products for both

Based on the fact that the clinical symptoms of Mycoplasma pneumoniae and Chlamydia pneumoniae infection are very similar, it is easy to miss the detection of Mycoplasma pneumoniae or Chlamydia pneumoniae antigen alone, or it needs to be tested twice for Chlamydia pneumoniae and Mycoplasma pneumoniae respectively, which is extremely inconvenient and the efficiency of clinical diagnosis is low. It is urgent to provide a detection equipment that can realize the joint detection of Chlamydia pneumoniae and Mycoplasma pneumoniae

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