36results about How to "Increase the rate of acid production" patented technology

The invention discloses a method for producing table vinegar by adopting a two-step acetic acid fermentation method and belongs to the technical field of brewing of the table vinegar. According to the method, the problems of low alcohol intolerance, long fermentation period and low acetification rate for acetic acid fermentation in a traditional method are solved. The method comprises the following steps of: uniformly stirring sorghum flour, corn flour, rice meal and water; adding thermostable alpha amylase for liquefying; after liquefying, reducing the temperature, adding Daqu, saccharifyingkoji, red starter, saccharomyces cerevisiae and aroma-producing yeast and carrying out alcoholic fermentation to obtain liquor; adopting the two-step acetic acid fermentation method: firstly, mixing the liquor; adding acetic acid bacteria subjected to liquid amplification culture for carrying out primary acetic acid fermentation; when the alcoholic strength of brewing mass is reduced to 6-5DEG C,adding wheat bran and rice husk as accessories for mixing; inoculating fire fermented liquor for carrying out secondary acetic acid fermentation; sealing the liquor; smoking the liquor; spraying vinegar; filtering; and packaging to obtain a finished product. According to the method disclosed by the invention, the high-content alcohol can be effectively converted; the acid yield and the acetification rate in unit time are improved; and the flavor of the table vinegar produced by adopting a traditional solid acetic acid fermentation method is ensured.

The invention discloses a fermenting type apple vinegar beverage and a preparation method thereof. The fermenting type apple vinegar beverage is characterized by being prepared by preparing, filtering, sterilizing and filling the following raw materials by weight proportion: 50-70 parts of apple vinegar, 80-120 parts of concentrated apple juice, 2-8 parts of honey, 1-3 parts of aspartame and 799-867 parts of water. The fermenting type apple vinegar beverage has the advantages of well retaining the original nutrient substances and flavor of apple, having high acid production rate, being obviously shortened in fermenting time, further being capable of separating cured acetic bacteria cells and products easily and being used repeatedly, enabling strains to be efficient, greatly improving production efficiency, saving production cost and reducing pollution discharging.

The invention belongs to the technical field of environmental protection, and relates to a method for improving short-chain fatty acid produced by excess sludge through hot alkali combined pretreatment. According to the method, the excess sludge in a sewage treatment plant serves as raw material, through hot pretreatment, controlling of initial alkaline power of hydrogen (PH) value conditions and the functions of microorganism is conducted, not only are non-aqueous resolvable organic matters in the sludge promoted to be dissolved and are the non-aqueous resolvable organic matters hydrolyzed to be converted to resolvable organic matters, but also the resolvable organic matters such as protein and polysaccharide are fully converted into the short-chain fatty acid, and pollution of the sludge to the environment is reduced. The temperature of the hot pretreatment is 60-120 DEG C, the time of the hot pretreatment is 15-60min, the initial PH value range of the functions of the microorganism is 8-12, the standing time of the sludge in a reactor is 1-12 days, and the temperature is 30-40 DEG C.

This invention relates to a construction method of tropic Candida gene engineering strain. Initial strain is product strain for industry, blocking one copy gene of carnitine acetyl CoA CAT and constructing a gene engineering recombination strain. At the same conditions, growth state of initial and recombination strain are same. CAT enzyme activity of recombination strain drops to half of that of initial strain. Speed rate of producing acid is improved. Crop of dibasic acid and percent conversion increases obviously.

The invention discloses a method of optimizing an acid production structure in an anaerobic fermentation acid production process of excess sludge and increasing a short chain fatty acid yield throughpretreatment of iron anode and electricity combined activation of persulfate. The method comprises the steps of: taking sludge as a fermentation substrate, a single-chamber electrolytic tank as a reaction device, an iron sheet as an anode and a graphite rod as a cathode, adding the sludge into an electrochemical reaction tank, adding persulfate into the fermentation substrate at the same time, electrifying for pretreatment, adjusting an initial pH (potential of hydrogen) to 10 for anaerobic fermentation, and obtaining structure optimized short chain volatile fatty acid. The method takes the sludge of a sewage treatment plant as a raw material; an acetic acid yield can be increased to the maximum extent by controlling an iron concentration, a current magnitude, electrifying time and a persulfate concentration in a pretreatment system; and the method contributes to achieving carbon source internal circulation of the sewage treatment plant and promoting sludge reclamation utilization. Atthe same time, the method also has the advantages of simplicity in operation, convenient management and high cost performance.

The invention provides a biomembrane-electrodialysis coupling continuous production technology of L-lactic acid. The production technology comprises the following steps: a clear liquid biomembrane column reactor is used to perform continuous anaerobic fermentation, the lactic acid producing strains are adsorbed by combined perforated ring filler, an ultrafiltration membrane is used to filter the fermentation liquor, thalli are blocked by the ultrafiltration membrane, the blocked thalli and liquid are sent back to the biomembrane column reactor, and L-lactic acid is separated through electrodialysis. The method provided by the invention uses the biomembrane column reactor to adsorb lactic acid producing strains on the membrane and uses the ultrafiltration membrane to block lactic acid bacteria, thus the thalli concentration and sugar utilization rate can be effectively increased, and the lactic acid concentration, the acid producing rate and the conversation rate from glucose to lacticacid can be increased greatly.

The invention discloses steamed buns with lactobacillus sanfrancisco and a preparation method thereof, and belongs to the technical field of food processing. The steamed buns with the lactobacillus sanfrancisco is prepared from the following raw materials in proportion by weight: 380-420 grams of wheat flour, 3-4 grams of Angel high-active dry yeast and 40-160 mL of lactobacillus sanfrancisco Ls-1001, wherein colony forming units of the lactobacillus sanfrancisco Ls-1001 is 3.80x10<13> to 4.00x10<13> CFU / mL. According to embodiments of the steamed buns with the lactobacillus sanfrancisco and the preparation method thereof, the steamed buns are loose and soft in taste, as well as appropriate in sour-sweet flavor, by adding the lactobacillus sanfrancisco Ls-1001 at different bacterium amount; moreover, the steamed buns have aroma of old dough fermented steamed buns in terms of sensory quality. According to the embodiments of the steamed buns with the lactobacillus sanfrancisco and the preparation method thereof, fermentation of the lactobacillus sanfrancisco Ls-1001 in the steamed buns is beneficially promoted by adding beer; and thus, the prepared steamed buns are loose and soft intaste, uniform in texture and mellow in old dough aroma.

The invention discloses a method for producing table vinegar by adopting a two-step acetic acid fermentation method and belongs to the technical field of brewing of the table vinegar. According to the method, the problems of low alcohol intolerance, long fermentation period and low acetification rate for acetic acid fermentation in a traditional method are solved. The method comprises the following steps of: uniformly stirring sorghum flour, corn flour, rice meal and water; adding thermostable alpha amylase for liquefying; after liquefying, reducing the temperature, adding Daqu, saccharifying koji, red starter, saccharomyces cerevisiae and aroma-producing yeast and carrying out alcoholic fermentation to obtain liquor; adopting the two-step acetic acid fermentation method: firstly, mixing the liquor; adding acetic acid bacteria subjected to liquid amplification culture for carrying out primary acetic acid fermentation; when the alcoholic strength of brewing mass is reduced to 6-5DEG C, adding wheat bran and rice husk as accessories for mixing; inoculating fire fermented liquor for carrying out secondary acetic acid fermentation; sealing the liquor; smoking the liquor; spraying vinegar; filtering; and packaging to obtain a finished product. According to the method disclosed by the invention, the high-content alcohol can be effectively converted; the acid yield and the acetification rate in unit time are improved; and the flavor of the table vinegar produced by adopting a traditional solid acetic acid fermentation method is ensured.

The invention discloses a vinegar processing method, which relates to the technical field of vinegar processing, comprising the following steps: (1) preparation of auxiliary fermentation liquid; (2) liquefaction of raw materials; (3) alcohol fermentation; (4) acetic acid fermentation; The vinegar processing method can effectively convert alcohol, improve the amount and rate of acid production per unit time, and ensure the flavor of traditional vinegar.

The invention discloses a fermenting type apple vinegar beverage and a preparation method thereof. The fermenting type apple vinegar beverage is characterized by being prepared by preparing, filtering, sterilizing and filling the following raw materials by weight proportion: 50-70 parts of apple vinegar, 80-120 parts of concentrated apple juice, 2-8 parts of honey, 1-3 parts of aspartame and 799-867 parts of water. The fermenting type apple vinegar beverage has the advantages of well retaining the original nutrient substances and flavor of apple, having high acid production rate, being obviously shortened in fermenting time, further being capable of separating cured acetic bacteria cells and products easily and being used repeatedly, enabling strains to be efficient, greatly improving production efficiency, saving production cost and reducing pollution discharging.

The present invention provides a kind of Lactobacillus plantarum with fast acid production and high acid production and application thereof, which is Lactobacillus plantarum ( Lactobacillus plantarum ) WG03, the preservation unit is China General Microorganism Culture Collection Management Center, the preservation number is CGMCC 20876, and the preservation date is October 12, 2020. The present invention provides a microbial inoculum comprising the above-mentioned Lactobacillus plantarum. The above-mentioned Lactobacillus plantarum or bacterial agent can be applied to the fermentation of silage. In the present invention, a strain of Lactobacillus plantarum with fast acid production and high acid production is obtained by protoplast fusion technology combined with traditional mutagenesis. Using this strain as a silage additive can well inhibit the growth of spoilage bacteria and quickly start Anaerobic fermentation process to improve the quality of silage.

The invention relates to a method for producing a short-chain fatty acid employing combined pretreatment of activated sludge. The method comprises the following steps: (1) carrying out gamma-ray irradiation on activated sludge, so as to prepare gamma-ray irradiated sludge; (2) carrying out ultrasonic treatment on the gamma-ray irradiated sludge, so as to prepare ultrasonic-treated sludge; and (3) adjusting the pH value of ultrasonic-treated sludge, carrying out anaerobic fermentation, so as to prepare the combined pretreated activated sludge. After the activated sludge is subjected to gamma-ray irradiation and ultrasonic treatment, organic matters such as protein and polysaccharide in the sludge dissolved into water can be significantly improved, so that an abundant organic matrix which can be dissolved into water is provided for fermentation and acidogenesis of sludge; the acid production rate of the sludge is improved; the anaerobic fermentation time of the sludge is shortened; and the method has important significance in improvement and optimization of an existing sludge treatment system, energy conservation and consumption reduction, and reduction of the operation cost.

The invention relates to a genetically engineered bacterium for producing alpha-ketobutyric acid as well as a construction method and application of the genetically engineered bacterium, belonging to the technical field of biology. The strain is relatively high in production efficiency when used for producing alpha-ketobutyric acid through fermentation. The genetically engineered bacterium is obtained through carrying out genetic engineering modification on Escherichia coli MG1655; the genetic engineering modification is realized by knocking out a large-subunit encoding gene ilvB of acetohydroxyacid synthase I, a large-subunit encoding gene ilvI of acetohydroxyacid synthase III and a threonine operon leading peptide encoding gene thrL in an over-expressive threonine dehydratase encoding gene ilvA. When the bacterium is used for producing alpha-ketobutyric acid by using a fermentation method, defects such as complex reaction conditions, high energy consumption, serious pollution or high production cost, serious pollution and the like existing in a chemical synthesis method, an enzyme method or a microbial conversion method can be overcome; after the bacterium is fermented for 20-24h, the yield of alpha-ketobutyric acid is up to 8.5-15.7g / L; aerobic fermentation is adopted in a fermentation process; the bacterium is rapid in growth, short in fermentation period and high in acid production rate; any reports for producing alpha-ketobutyric acid by using a direct fermentation method are not found at present.

The invention discloses a method for continuous cultivation of Aspergillus niger seeds. The method comprises the following steps: (1) in the start-up stage, inoculating the Aspergillus niger spores into the seed medium to obtain a seed liquid; (2) in the continuous cultivation stage of the seeds, The seed solution obtained in step (1) is subjected to continuous dispersion treatment, and the seed solution obtained by dispersing is continuously cultivated, and fresh seed feed medium is supplemented at the same time; (3) stop stage, stop supplementing fresh seed feed medium and disperse treatment, Continue culturing to obtain seed liquid, which is transferred into the fermentation medium for fermentation and cultivation. The method of the present invention solves the problem of slow growth of multicellular filamentous bacteria and easy loss of mycelium balls in continuous culture, completely realizes the continuous cultivation of seeds, keeps the bacteria in the most suitable growth environment, and avoids the Seed degradation, so that the seed liquid is in a continuous and stable high-activity state, corresponding to the improvement of the performance of citric acid production by fermentation.

The invention discloses a method for promoting production of fermentation acid from remaining sludge by utilizing redox mediator. The method comprises the steps of stirring the remaining sludge by an underflow stirrer for 1-6h at the stirring speed of 30-100rpm under anaerobic conditions, adding redox mediator stock solution to enable the concentration of redox mediator in a fermentation system to be 0.5-200mg / L and performing production of fermentation acid in a fermentation tank or an anaerobic reactor; the fermentation conditions are as follows: the fermentation temperature is controlled to be 15-35 DEG C, the pH value of the fermentation system is regulated to 6.0-12.0, the redox potential is controlled to be below -100mV, the underflow stirrer is adopted for stirring, the stirring speed is set to be 50-200rpm and the fermentation time is 3-30d. The remaining sludge can be directly mixed with redox mediator without alkali, hot alkali or ultrasonic pretreatment and volatile fatty acids can be fast and efficiently produced by controlling the operation conditions disclosed by the invention.