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Method for improving acid production rate and conversion rate of butyric acid fermentation

A conversion rate and butyric acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of acid production rate and product conversion rate to be improved, and the control is not fine enough, so as to increase the content of butyric acid and conversion rate, cycle shortening, and the effect of increasing acid production rate

Inactive Publication Date: 2020-07-03
ZHUMADIAN HUAZHONG CHIA TAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing fermentation ORP control range is relatively wide, but the control is not fine enough, and the acid production rate and product conversion rate need to be improved

Method used

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  • Method for improving acid production rate and conversion rate of butyric acid fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The first-level seed culture process: temperature 37℃, anaerobic, rotation speed 200rpm, natural pH, culture time 20-24h.

[0024] The ORP value is changed by passing sterile air into the seed tank (the amount of sterile air is 5% of the volume of the material), and the fermentation tank is not allowed to pass sterile air for normal culture, that is, the ORP is -400~- for secondary seed culture. 200, temperature 37℃, rotation speed 200rpm, culture period 18h. During fermentation, the ORP is -600~-300, the temperature is 37℃, the speed is 200rpm, the culture period is 48-51h, and the pH is controlled at 5.90. After the fermentation, the butyric acid content was determined.

[0025] For primary seed culture and secondary seed culture, the seed growth medium used is: glucose 20g / L, yeast extract 2g / L, peptone 4g / L, (NH 4 ) 2 SO 4 2g / L, K 2 HPO 4 1g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.1g / L, FeSO 4 ·7H 2 O 0.015g / L, CaCl 2 ·2H 2 O 0.015g / L, MnSO4·H 2 O 0.01g / L, CoCl 2 ·6H 2 O...

Embodiment 2

[0029] The seed tank is cultured normally, and the sterile air is passed into the fermentor to change the ORP value (the amount of sterile air is 8% of the material volume), that is, the ORP of the secondary seed culture is -500~-300, the temperature is 37°C, and the rotation speed 200rpm, 18h culture period, pH control at 5.90. The ORP of fermentation culture is -450~-350, the temperature is 37℃, the rotation speed is 200rpm, the culture period is 49h, and the pH is controlled at 5.90. After the fermentation, the butyric acid content was determined.

Embodiment 3

[0031] Sterile air is introduced separately (the amount of sterile air in the seed tank is 5% of the volume of the material, and the amount of sterile air in the fermenter is 8% of the volume of the material), that is, the ORP of secondary seed culture is- 400~-200, speed 200rpm, culture period 18h, pH control at 5.90. The ORP of fermentation culture is -450~-350, the temperature is 37℃, the rotation speed is 200rpm, the culture period is 49h, and the pH is controlled at 5.90. After the fermentation, the butyric acid content was determined.

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Abstract

The invention provides a method for improving the acid production rate and conversion rate of butyric acid fermentation, and relates to the technical field of butyric acid fermentation. According to the method disclosed by the invention, a new ORP control standard is utilized; the ORP range standard of secondary seed culture is minus 400 to minus 200; and the ORP range standard of fermentation culture is minus 450 to minus 350. By utilization of the method disclosed by the invention, the period is shortened; the butyric acid content and conversion rate are improved; and compared with the priorart, the method improves the acid production rate by 9.9%.

Description

Technical field [0001] The invention belongs to the technical field of butyric acid fermentation, and specifically relates to a method for improving the acid production rate and conversion rate of butyric acid fermentation. Background technique [0002] Butyric acid fermentation is an anaerobic fermentation system (using nitrogen). During the fermentation process, pH, ORP (oxidation reduction potential), and pH are controlled to control the fermentation. Existing fully anaerobic fermentation system, first-level seed culture process: temperature 37℃, anaerobic, rotation speed 200rpm, natural pH, culture time 20-24h; second-level seed culture: temperature 37℃, rotation speed 200rpm, culture period 10-24h , ORP control -300 to -500, pH control at 5.90; Fermentation culture: temperature 37℃, rotation speed 150-200rpm, culture period 40-55h, ORP control -300 to -600, pH control at 5.90. The existing fermentation ORP control range is wide and the control is not precise enough, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/52C12R1/145
CPCC12N1/20C12P7/52
Inventor 娄百勇吕向云李冰曹华伟李书至杨永杨守锋李学成
Owner ZHUMADIAN HUAZHONG CHIA TAI
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